Abstract：
:After injury to the mature central nervous system (CNS), myelin-derived inhibitory ligands bind to the Nogo-66 tripartite receptor complex expressed on axonal growth cones, comprised of LINGO-1 and p75NTR/TROY and induce growth cone collapse through the RhoA pathway. We have also shown that amphoterin-induced gene and open reading frame-3 (AMIGO3) substitutes for LINGO-1 and can signal axon growth cone collapse. Here, we investigated the regeneration of dorsal root ganglion neuron (DRGN) axons/neurites after treatment with a short hairpin RNA (sh) AMIGO3 plasmid delivered with a non-viral in vivo-jetPEI vector, and the pro-survival/axogenic neurotrophin (NT) 3 in vitro and in vivo. A bicistronic plasmid, containing both shAMIGO3 and NT3 knocked down >75% of AMIGO3 mRNA in cultured DRGN and significantly overexpressed NT3 production. In vivo, intra-DRG injection of in vivo-jetPEI plasmids containing shAMIGO3/gfp and shAMIGO3/nt3 both knocked down AMIGO3 expression in DRGN and, in combination with NT3 overexpression, promoted DC axon regeneration, recovery of conduction of compound action potentials across the lesion site and improvements in sensory and locomotor function. These findings demonstrate that in vivo-jetPEI is a potential non-viral, translatable DRGN delivery vehicle in vivo and that suppression of AMIGO3 disinhibits the growth of axotomised DRGN enabling NT3 to stimulate the regeneration of their DC axons and enhances functional recovery.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Almutiri S,Berry M,Logan A,Ahmed Zdoi
10.1038/s41598-018-29124-zsubject
Has Abstractpub_date
2018-07-16 00:00:00pages
10707issue
1issn
2045-2322pii
10.1038/s41598-018-29124-zjournal_volume
8pub_type
杂志文章abstract：:The natural bacterial diversity is regarded as a treasure trove for natural products. However, accessing complex cell mixtures derived from environmental samples in standardized high-throughput screenings is challenging. Here, we present a droplet-based microfluidic platform for ultrahigh-throughput screenings able to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31263-2
更新日期：2018-08-30 00:00:00
abstract：:Algicidal microbes could effectively remove the harmful algae from the waters. In this study, we were concerned with the ecological influence of an algicide extracted from Streptomyces alboflavus RPS, which could completely lyse the Phaeocystis globosa cells within two days. In microcosms, 4 μg/mL of the microbial alg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20081
更新日期：2016-02-05 00:00:00
abstract：:Relative permeability and capillary pressure are the governing parameters that characterize multiphase fluid flow in porous media for diverse natural and industrial applications, including surface water infiltration into the ground, CO2 sequestration, and hydrocarbon enhanced recovery. Although the drastic effects of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51263-0
更新日期：2019-10-18 00:00:00
abstract：:Accumulating evidence suggests that the gut microbiota dysbiosis and their host metabolic phenotype alteration is an important factor in human disease development. A traditional Chinese herbal formula, Chaihu-Shu-Gan-San (CSGS), has been effectively used in the treatment of various gastrointestinal (GI) disorders. The...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46551
更新日期：2017-04-20 00:00:00
abstract：:Traditionally, dynamic atomic force microscopy (AFM) techniques are based on the analysis of the quasi-steady state response of the cantilever deflection in terms of Fourier analysis. Here we describe a technique that instead exploits the often disregarded transient response of the cantilever through a relatively mode...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49104-1
更新日期：2019-09-03 00:00:00
abstract：:Posttraumatic growth (PTG) is defined as positive psychological change in the wake of highly challenging circumstances. Healthcare workers in particular are more vulnerable to stressors and trauma than the general population. The current study examined the use and effectiveness of a novel positive psychological interv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39189
更新日期：2016-12-20 00:00:00
abstract：:Glutamate (Glu), the key excitatory neurotransmitter in the central nervous system, is considered essential for brain functioning and has a vital role in learning and memory formation. Earlier it was considered as a harmful agent but later found to be useful for many body functions. However, studies regarding the effe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68041-y
更新日期：2020-07-08 00:00:00
abstract：:Our mind's eye and the role of internal attention in mental life and suffering has intrigued scholars for centuries. Yet, experimental study of internal attention has been elusive due to our limited capacity to control the timing and content of internal stimuli. We thus developed the Simulated Thoughts Paradigm (STP) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81756-w
更新日期：2021-01-26 00:00:00
abstract：:Metasurfaces, owing to their attractive features, provide a wide range of potential applications. Electromagnetic absorbers based on metasurfaces have significantly improved responses compared to the earlier absorbers made from composite materials. Active metasurfaces, in contrast to the passive designs, can exhibit m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28574-9
更新日期：2018-07-05 00:00:00
abstract：:HAUSP (herpes virus-associated ubiquitin specific protease, known as ubiquitin specific protease 7), one of DUBs, regulates the dynamics of the p53 and Mdm2 network in response to DNA damage by deubiquitinating both p53 and its E3 ubiquitin ligase, Mdm2. Its concerted action increases the level of functional p53 by pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12793
更新日期：2015-08-04 00:00:00
abstract：:Histone acetylation has been linked to cardiac hypertrophy and heart failure. However, the pathological implications of changes in histone methylation and the effects of interventions with histone methyltransferase inhibitors for heart failure have not been fully clarified. Here, we focused on H3K9me3 status in the he...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39752
更新日期：2017-01-04 00:00:00
abstract：:Si holds great promise as an alloying anode material for Li-ion batteries with improved energy density because of its high theoretical specific capacity and favorable operation voltage range. However, the large volume expansion of Si during electrochemical reaction with Li and the associated adverse effects strongly l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13261-y
更新日期：2017-10-12 00:00:00
abstract：:Despite recent advances, the myocardial microstructure remains imperfectly understood. In particular, bundles of cardiomyocytes have been observed but their three-dimensional organisation remains debated and the associated mechanical consequences unknown. One of the major challenges remains to perform multiscale obser...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76820-w
更新日期：2020-11-25 00:00:00
abstract：:For lung and many other cancers, prognosis is essentially important, and extensive modeling has been carried out. Cancer is a genetic disease. In the past 2 decades, diverse molecular data (such as gene expressions and DNA mutations) have been analyzed in prognosis modeling. More recently, histopathological imaging da...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72201-5
更新日期：2020-09-14 00:00:00
abstract：:Systemic antioxidative status has been implicated in glaucoma pathogenesis. Additionally, corneal hysteresis (CH) may contribute to glaucoma progression. Here, we evaluated the relationship between biological antioxidant potential (BAP) and CH. This study included 103 patients with open-angle glaucoma (OAG). We used a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57520-x
更新日期：2020-01-17 00:00:00
abstract：:A number of studies have shown that visible and near infrared spectroscopy (VIS-NIRS) offers a rapid on-site measurement tool for the determination of total contaminant concentration of petroleum hydrocarbons compounds (PHC), heavy metals and metalloids (HM) in soil. However none of them have yet assessed the feasibil...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41161-w
更新日期：2019-03-14 00:00:00
abstract：:Natural variability in pH in the diffusive boundary layer (DBL), the discrete layer of seawater between bulk seawater and the outer surface of organisms, could be an important factor determining the response of corals and coralline algae to ocean acidification (OA). Here, two corals with different morphologies and one...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49044-w
更新日期：2019-09-06 00:00:00
abstract：:Traumatic brain injury (TBI) increases the risk of Alzheimer's disease (AD). Calpain activation and tau hyperphosphorylation have been implicated in both TBI and AD. However, the link between calpain and tau phosphorylation has not been fully identified. We recently discovered that the two major calpain isoforms in th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12236-3
更新日期：2017-09-18 00:00:00
abstract：:CRISPR/Cas9-based gene knockout in animal cells, particularly in teleosts, has proven to be very efficient with regards to mutation rates, but the precise insertion of exogenous DNA or gene knock-in via the homology-directed repair (HDR) pathway has seldom been achieved outside of the model organisms. Here, we succeed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79409-5
更新日期：2020-12-17 00:00:00
abstract：:The oral cavity contains different types of microbial species that colonize human host via extensive cell-to-cell interactions and biofilm formation. Candida albicans-a yeast-like fungus that inhabits mucosal surfaces-is also a significant colonizer of subgingival sites in patients with chronic periodontitis. It is no...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40771-8
更新日期：2019-03-13 00:00:00
abstract：:In this work, lamellar MnFe2O4 was successfully planted on a wood surface through the association of hydrogen bonds via the one-pot hydrothermal method. Simultaneously, the fluoroalkylsilane (FAS-17) on the surface of the MnFe2O4 layer formed long-chain or network macromolecules through a poly-condensation process and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35549
更新日期：2016-10-17 00:00:00
abstract：:Recent findings suggest an association between obesity, loss of gut barrier function and changes in microbiota profiles. Our primary objective was to examine the effect of caloric restriction and subsequent weight reduction on gut permeability in obese women. The impact on inflammatory markers and fecal microbiota was...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12109-9
更新日期：2017-09-20 00:00:00
abstract：:In HIV-1/HCV-coinfected patients, chronic HCV infection leads to an increased T-lymphocyte immune activation compared to HIV-monoinfected patients, thereby likely contributing to increase HIV-1 reservoir that is the major barrier for its eradication. Our objective was to evaluate the influence of HCV coinfection in HI...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41788-9
更新日期：2019-04-03 00:00:00
abstract：:Global biodiversity hotspots (GBHs) are increasingly vulnerable to human stressors such as anthropogenic climate change, which will alter the ecology of these habitats, even where protected. The longleaf pine (Pinus palustris) ecosystem (LPE) of the North American Coastal Plain is a GBH where disturbances are integral...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65436-9
更新日期：2020-05-21 00:00:00
abstract：:Supplementation of cattle diets with n-3-polyunsaturated fatty acids (PUFA) can improve reproductive efficiency. Conversely, short-term fluctuations in feed supply can impact pregnancy establishment. The objectives of this study were to examine the effects of (1) dietary supplementation with n-3-PUFA and (2) post-inse...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77604-y
更新日期：2020-11-27 00:00:00
abstract：:Systemic Sclerosis (SSc) is a disease with limited therapeutic possibilities. Mesenchymal stem cells (MSCs)-therapy could be a promising therapeutic option, however the ideal MSCs source has not yet been found. To address this problem, we perform comparison between bone marrow (BM)-MSCs and adipose (A)-MSCs, by the mi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43638-0
更新日期：2019-05-09 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-24103-w
更新日期：2018-04-13 00:00:00
abstract：:The interest in nanostructures of silicon and its dopants has significantly increased. We report the creation of an ultimately-shallow junction at the surface of n-type silicon with excellent electrical and optical characteristics made by depositing an atomically thin boron layer at a relatively low temperature where ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13100-0
更新日期：2017-10-16 00:00:00
abstract：:Social context influences social decisions and outcome processing, partially depending on inter-individual differences. The present study investigated social context-dependent modulation of behavior and feedback processing in the ultimatum game (UG) in relation to inter-individual differences in social anxiety. Thirty...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/srep44062
更新日期：2017-03-09 00:00:00
abstract：:Layered Li2RuO3 is an important candidate cathode material in rechargeable lithium ion batteries because of its novel anionic redox process and high reversible capacity. Atomistic scale simulations are used to calculate the intrinsic defect process, favourable dopants and migration energies of lithium ion diffusions t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36865-4
更新日期：2019-01-24 00:00:00
