Abstract:
:Glutaredoxins (GRXs), small redox proteins that use reduced glutathione as an electron donor, are key components of the cellular antioxidant system. In this study, we used Saccharomyces cerevisiae as a model system to investigate the effects of GRX deletion on yeast chronological life span (CLS). Deletion of either Grx1 or Grx2 shortened yeast CLS. Quantitative proteomics revealed that GRX deletion decreased the expression of stress-response proteins, leading to increased cellular reactive oxygen species accumulation and, subsequently, intracellular acidification. This activated the Ras/protein kinase A (PKA) signaling pathway. Genetic and biochemical analyses demonstrated that Ras/PKA activation decreased stress resistance and increased biosynthesis, requiring yeast cells to grow under unfavorable conditions and resulting in a shortened CLS. Our results provided new insights into mechanisms underlying exacerbation of the aging process by oxidative stress.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Liu Y,Yang F,Li S,Dai J,Deng Hdoi
10.1021/acs.jproteome.8b00012subject
Has Abstractpub_date
2018-07-06 00:00:00pages
2318-2327issue
7eissn
1535-3893issn
1535-3907journal_volume
17pub_type
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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doi:10.1021/pr500202e
更新日期:2014-08-01 00:00:00
abstract::Clostridioides difficile-associated infection (CDI) is a health-care-associated infection caused, as the name suggests, by obligate anaerobic pathogen C. difficile and thus mainly transmitted via highly resistant endospores from one person to the other. In vivo, the spores need to germinate into cells prior to establi...
journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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doi:10.1021/pr049886m
更新日期:2004-11-01 00:00:00
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更新日期:2009-11-01 00:00:00
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journal_title:Journal of proteome research
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doi:10.1021/pr401181y
更新日期:2014-06-06 00:00:00
abstract::Diabetes mellitus is a complex polygenic disease caused by gene-environment interactions with multiple complications, and metabonomic analysis is crucial for pathogenesis, early diagnosis, and timely interventions. Here, we comprehensively analyzed the dynamic metabolic changes in rat urine and plasma, which were indu...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr300280t
更新日期:2012-06-01 00:00:00
abstract::One-hundred twenty-four proteins have been identified in the soluble nuclear protein mixture from MCF-7 human breast cancer cells, of which more than 90% are classically categorized as nuclear proteins. Proteins were also studied from three drug resistant MDF-7 lines, selected previously from the same parent line by e...
journal_title:Journal of proteome research
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abstract::Oxidative stress and inflammation play a role in the physiopathology of insulin resistance, diabetes and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC) meal induces an increase in inflammatory and oxidative stress markers in peripheral blood mononuclear cells (PBMC). Previous studies have shown th...
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100789v
更新日期:2010-12-03 00:00:00
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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