Abstract：
:Connective tissues such as tendon, ligament and cartilage are mostly composed of extracellular matrix (ECM). These tissues are insoluble, mainly due to the highly cross-linked ECM proteins such as collagens. Difficulties obtaining suitable samples for mass spectrometric analysis render the application of modern proteomic technologies difficult. Complete solubilization of them would not only elucidate protein composition of normal tissues but also reveal pathophysiology of pathological tissues. Here we report complete solubilization of human Achilles tendon and yellow ligament, which is achieved by chemical digestion combined with successive protease treatment including elastase. The digestion mixture was subjected to liquid chromatography-mass spectrometry. The low specificity of elastase was overcome by accurate mass analysis achieved using FT-ICR-MS. In addition to the detailed proteome of both tissues, we also quantitatively determine the major protein composition of samples, by measuring peak area of some characteristic peptides detected in tissue samples and in purified proteins. As a result, differences between human Achilles tendon and yellow ligament were elucidated at molecular level.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Sato N,Taniguchi T,Goda Y,Kosaka H,Higashino K,Sakai T,Katoh S,Yasui N,Sairyo K,Taniguchi Hdoi
10.1021/acs.jproteome.6b00806subject
Has Abstractpub_date
2016-12-02 00:00:00pages
4709-4721issue
12eissn
1535-3893issn
1535-3907journal_volume
15pub_type
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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doi：10.1021/acs.jproteome.0c00486
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2009-05-01 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.6b00584
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2011-07-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2011-12-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr200527z
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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