Correlating structural and photochemical heterogeneity in cyanobacteriochrome NpR6012g4.

abstract：:The Ser52Pro variant of transthyretin (TTR) produces aggressive, highly penetrant, autosomal-dominant systemic amyloidosis in persons heterozygous for the causative mutation. Together with a minor quantity of full-length wild-type and variant TTR, the main component of the ex vivo fibrils was the residue 49-127 fragme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mangione PP,Porcari R,Gillmore JD,Pucci P,Monti M,Porcari M,Giorgetti S,Marchese L,Raimondi S,Serpell LC,Chen W,Relini A,Marcoux J,Clatworthy IR,Taylor GW,Tennent GA,Robinson CV,Hawkins PN,Stoppini M,Wood SP,Pepys

更新日期：2014-01-28 00:00:00

abstract：:We have developed an efficient method to target lentivirus-mediated gene transduction to a desired cell type. It involves incorporation of antibody and fusogenic protein as two distinct molecules into the lentiviral surface. The fusogen is constructed by modifying viral envelope proteins, so that they lack the ability...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang L,Bailey L,Baltimore D,Wang P

更新日期：2006-08-01 00:00:00

abstract：:Interleukin-12 (IL-12) is critical for induction of protective immunity against intracellular bacterial infection. However, the mechanisms for efficient induction of IL-12 in innate response remain poorly understood. Here we report that the B type of carbonic anhydrase 6 (Car6-b, which encoded CA-VI B) is essential fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu J,Xu X,Wang B,Ma Y,Zhang L,Xu H,Hu Y,Wu J,Cao X

更新日期：2017-08-08 00:00:00

abstract：:The complex prokaryote, Myxococcus xanthus, undergoes a program of multicellular development when starved for nutrients, culminating in sporulation. M. xanthus makes MglA, a 22-kDa, soluble protein that is required for both multicellular development and gliding motility. MglA is similar in sequence to the Saccharomyce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hartzell PL

更新日期：1997-09-02 00:00:00

abstract：:We have shown recently that the temporal order of antioxidant consumption in human blood plasma exposed to a constant flux of aqueous peroxyl radicals is ascorbate = protein thiols greater than bilirubin greater than urate greater than alpha-tocopherol and that detectable lipid peroxidation starts only after ascorbate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frei B,England L,Ames BN

更新日期：1989-08-01 00:00:00

abstract：:A cDNA sequence coding for murine granulocyte colony-stimulating factor (G-CSF) has been isolated from a cDNA library prepared with mRNA derived from murine fibrosarcoma NFSA cells, which produce G-CSF constitutively. Identification of murine G-CSF cDNA was based on the cross-hybridization with human G-CSF cDNA under ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsuchiya M,Asano S,Kaziro Y,Nagata S

更新日期：1986-10-01 00:00:00

abstract：:By double indirect immunofluorescence, using primary rabbit antibodies to tubulin and guinea pig antibodies to vimentin, we have simultaneously labeled microtubules and intermediate filaments in several types of cultured normal fibroblasts. With well-spread interphase cells there was an extensive but not complete corr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ball EH,Singer SJ

更新日期：1981-11-01 00:00:00

abstract：:O(6)-methylguanine (O(6)meG) and related modifications of guanine in double-stranded DNA are functionally severe lesions that can be produced by many alkylating agents, including N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a potent carcinogen. O(6)meG is repaired through its demethylation by the O(6)-alkylguanine-DNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang CS,Shemorry A,Varshavsky A

更新日期：2009-02-17 00:00:00

abstract：:The defining feature of the mycobacterial outer membrane (OM) is the presence of mycolic acids (MAs), which, in part, render the bilayer extremely hydrophobic and impermeable to external insults, including many antibiotics. Although the biosynthetic pathway of MAs is well studied, the mechanism(s) by which these lipid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu Z,Meshcheryakov VA,Poce G,Chng SS

更新日期：2017-07-25 00:00:00

abstract：:Individual diastereomeric phosphoramidites and mixtures of diastereomeric phosphoramidites were evaluated in the iridium-catalyzed amination of allylic carbonates. The original process was conducted with a phosphoramidite ligand containing a resolved 2,2-dihydroxy-1,1-binaphthyl (BINOL) group and a diastereomerically ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leitner A,Shu C,Hartwig JF

更新日期：2004-04-20 00:00:00

abstract：:Associative memory of the mollusc Hermissenda crassicornis, previously correlated with changes of specific K+ currents, protein phosphorylation, and increased synthesis of mRNA and specific proteins, is here shown to be accompanied by macroscopic alteration in the structure of a single identified neuron, the medial ty...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alkon DL,Ikeno H,Dworkin J,McPhie DL,Olds JL,Lederhendler I,Matzel L,Schreurs BG,Kuzirian A,Collin C

更新日期：1990-02-01 00:00:00

abstract：:The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized "late" polyoma-specific RNA appears as "giant" molecules of heterogeneous size, up to several times large...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acheson NH,Buetti E,Scherrer K,Weil R

更新日期：1971-09-01 00:00:00

abstract：:High-throughput biological data, whether generated as sequencing, transcriptional microarrays, proteomic, or other means, continues to require analytic methods that address its high dimensional aspects. Because the computational part of data analysis ultimately identifies shape characteristics in the organization of d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nicolau M,Levine AJ,Carlsson G

更新日期：2011-04-26 00:00:00

abstract：:Erickson [Erickson CL (2000) Nature 408 (6809):190-193] interpreted features in seasonal floodplains in Bolivia's Beni savannas as vestiges of pre-European earthen fish weirs, postulating that they supported a productive, sustainable fishery that warranted cooperation in the construction and maintenance of perennial s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKey DB,Durécu M,Pouilly M,Béarez P,Ovando A,Kalebe M,Huchzermeyer CF

更新日期：2016-12-27 00:00:00

abstract：:The neu gene is distantly related to the erbB gene and encodes a cell surface protein that appears to function as a growth factor receptor. To study the mechanisms that caused the conversion of the normal neu gene to an oncogenic allele, we have isolated molecular clones of the neu oncogene as well as a clone of the c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hung MC,Schechter AL,Chevray PY,Stern DF,Weinberg RA

更新日期：1986-01-01 00:00:00

abstract：:neurogenin2 (ngn2) encodes a basic helix-loop-helix transcription factor and plays an important role in neurogenesis from migratory neural crest cells. Its role in retinal development is poorly understood. We observed that in the developing chick retina, ngn2 was expressed in a subpopulation of proliferating progenito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan RT,Ma WX,Wang SZ

更新日期：2001-12-18 00:00:00

abstract：:Charybdotoxin (ChTX), a K+ channel blocker, depolarizes human peripheral T lymphocytes and renders them insensitive to activation by mitogen. We observed four types of K+ channels in human T cells: one voltage-activated, and three Ca(2+)-activated. To discern the mechanism by which ChTX depolarizes T cells, we examine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leonard RJ,Garcia ML,Slaughter RS,Reuben JP

更新日期：1992-11-01 00:00:00

abstract：:Gene expression has a stochastic component because of the single-molecule nature of the gene and the small number of copies of individual DNA-binding proteins in the cell. We show how the statistics of such systems can be mapped onto quantum many-body problems. The dynamics of a single gene switch resembles the spin-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasai M,Wolynes PG

更新日期：2003-03-04 00:00:00

abstract：:The complexed (ribosome-bearing) membrane fraction of Bacillus subtilis contains several proteins (CM-proteins) that are virtually absent from the ribosome-free fraction and hence might be components of the apparatus of protein secretion. We have determined, by trypsin digestion and by labeling with a nonpenetrating r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horiuchi S,Tai PC,Davis BD

更新日期：1983-06-01 00:00:00

abstract：:3-Methylcholanthrene (MCA) skin painting rapidly induced a 100% incidence of thymic lymphomas in RF/J mice. Tumors developed exclusively in cells of the T-lymphocyte lineage and displayed the surface phenotype: Thy-1+, Lyt-1+, Lyt-2+, Qa-1+, H-2K+, H-2D+, TL-, Ig-, Ia-. This phenotype resembled that of cortisone-resis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodenow MM,Lilly F

更新日期：1984-12-01 00:00:00

abstract：:The six members of the contactin (CNTN) family of neural cell adhesion molecules are involved in the formation and maintenance of the central nervous system (CNS) and have been linked to mental retardation and neuropsychiatric disorders such as autism. Five of the six CNTNs bind to the homologous receptor protein tyro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lamprianou S,Chatzopoulou E,Thomas JL,Bouyain S,Harroch S

更新日期：2011-10-18 00:00:00

abstract：:A pure population of mast cells was obtained after 14 days of culturing mouse bone marrow cells in the presence of medium derived from concanavalin A-stimulated mouse spleen cells. The cells were characterized as mast cells by their morphologic appearance and histologic staining, by their histamine content (450 ng per...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razin E,Cordon-Cardo C,Good RA

更新日期：1981-04-01 00:00:00

abstract：:The guanosine hydrazide 1 yields a stable supramolecular hydrogel based on the formation of a guanine quartet (G-quartet) in presence of metal cations. The effect of various parameters (concentration, nature of metal ion, and temperature) on the properties of this gel has been studied. Proton NMR spectroscopy is shown...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sreenivasachary N,Lehn JM

更新日期：2005-04-26 00:00:00

abstract：:In diabetes, the death of insulin-producing beta-cells by apoptosis leads to insulin deficiency. The lower prevalence of diabetes in females suggests that female sex steroids protect from beta-cell injury. Consistent with this hypothesis, 17beta-estradiol (estradiol) manifests antidiabetic actions in humans and rodent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le May C,Chu K,Hu M,Ortega CS,Simpson ER,Korach KS,Tsai MJ,Mauvais-Jarvis F

更新日期：2006-06-13 00:00:00

abstract：:A mutant of E. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to replicate DNA of various sources was analyzed. Mutant bacteria grow normally, at 41 degrees as well as at 30 degrees . Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirota Y,Gefter M,Mindich L

更新日期：1972-11-01 00:00:00

abstract：:Studies to elucidate the mode of action of aldosterone have been carried out in the amphibian urinary bladder. The following previously reported hypotheses were evaluated: (1) aldosterone stimulates sodium transport by increasing the amount of sodium available to the sodium pump; (2) aldosterone enhances energy produc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodman DB,Allen JE,Rasmussen H

更新日期：1969-09-01 00:00:00

abstract：:Prochlorococcus is an abundant marine cyanobacterium that grows rapidly in the environment and contributes significantly to global primary production. This cyanobacterium coexists with many cyanophages in the oceans, likely aided by resistance to numerous co-occurring phages. Spontaneous resistance occurs frequently i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Avrani S,Lindell D

更新日期：2015-04-28 00:00:00

abstract：:We have developed an efficient, versatile, and user-friendly viral engineering and expression system that is based on in planta assembly of functional viral vectors from separate pro-vector modules. With this new system, instead of supplying a plant cell with a complete viral vector as a mature viral particle, an RNA ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marillonnet S,Giritch A,Gils M,Kandzia R,Klimyuk V,Gleba Y

更新日期：2004-05-04 00:00:00

abstract：:The growth of a small number of cells from each of two chemically induced BALB/c sarcomas was enhanced when x-irradiated (15,000 rads) cells of the same sarcoma were mixed with the tumor inoculum. This enhancement did not occur if the recipients had been given a total body x-irradiation of 450 rads. Tumor neutralizati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hellström KE,Hellström I

更新日期：1978-01-01 00:00:00

abstract：:Histone methyltransferase G9a has critical roles in promoting cancer-cell growth and gene suppression, but whether it is also associated with the DNA damage response is rarely studied. Here, we report that loss of G9a impairs DNA damage repair and enhances the sensitivity of cancer cells to radiation and chemotherapeu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Q,Zhu Q,Lu X,Du Y,Cao L,Shen C,Hou T,Li M,Li Z,Liu C,Wu D,Xu X,Wang L,Wang H,Zhao Y,Yang Y,Zhu WG

更新日期：2017-07-25 00:00:00