当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Characterization of Conserved and Novel microRNAs in Lilium lancifolium Thunb. by High-Throughput Sequencing.

Characterization of Conserved and Novel microRNAs in Lilium lancifolium Thunb. by High-Throughput Sequencing.

Abstract：

:MicroRNAs (miRNAs) are among the class of noncoding small RNA molecules and play a crucial role in post-transcriptional regulation in plants. Although Lilium is one of the most popular ornamental flowers worldwide, however, there is no report on miRNAs identification. In the present study, therefore, miRNAs and their targets were identified from flower, leaf, bulblet and bulb of Lilium lancifolium Thunb. by high-throughput sequencing and bioinformatics analysis. In this study, a total of 38 conserved miRNAs belonging to 17 miRNA families and 44 novel miRNAs were identified. In total, 366 target genes for conserved miRNAs and 415 target genes for novel miRNAs were predicted. The majority of the target genes for conserved miRNAs were transcriptional factors and novel miRNAs targeted mainly protein coding genes. A total of 53 cleavage sites belonging to 6 conserved miRNAs families and 14 novel miRNAs were identified using degradome sequencing. Twenty-three miRNAs were randomly selected, then, their credibility was confirmed using northern blot or stem-loop qRT-PCR. The results from qRT-PCR analysis showed the expression pattern of 4 LL-miRNAs was opposite to their targets. Therefore, our finding provides an important basis to understand the biological functions of miRNAs in Lilium.

journal_name

Sci Rep

journal_title

Scientific reports

authors

He X,Shenkute AG,Wang W,Xu S

doi

10.1038/s41598-018-21193-4

subject

Has Abstract

pub_date

2018-02-13 00:00:00

pages

2880

issue

1

issn

2045-2322

pii

10.1038/s41598-018-21193-4

journal_volume

8

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions.

    abstract：:Karrikins (KAR) are a class of signal compounds, discovered in wildfire smoke, which affect seed germination. Currently, numerous studies have focused on the model plant Arabidopsis in the KAR research field, rather than on crops. Thus the regulatory mechanisms underlying KAR regulation of crop seed germination are la...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep22073

    authors： Meng Y,Chen F,Shuai H,Luo X,Ding J,Tang S,Xu S,Liu J,Liu W,Du J,Liu J,Yang F,Sun X,Yong T,Wang X,Feng Y,Shu K,Yang W

    更新日期：2016-02-23 00:00:00

  • Gender differences in the influence of obstructive sleep apnea on optic nerve head circulation.

    abstract：:We investigated gender differences in the optic nerve head (ONH) microcirculation status in association with obstructive sleep apnea (OSA) by using laser speckle flowgraphy (LSFG). We evaluated 150 men (60.5 ± 11.0 yrs) and 45 women (63.0 ± 10.6 yrs) who underwent overnight polysomnography. The mean blur rate (MBR), m...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-55470-7

    authors： Shiba T,Takahashi M,Matsumoto T,Hori Y

    更新日期：2019-12-11 00:00:00

  • microRNA Expression in Ethnic Specific Early Stage Breast Cancer: an Integration and Comparative Analysis.

    abstract：:Breast cancer (BC) has a higher incidence in young Lebanese woman as compared to the West. We assessed the microRNA (miRNA) microarray profile of tissues derived from Lebanese patients with early BC and performed mRNA-miRNA integration analysis. 173 miRNAs were significantly dysregulated in 45 BC versus 17 normal adja...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-16978-y

    authors： Nassar FJ,Talhouk R,Zgheib NK,Tfayli A,El Sabban M,El Saghir NS,Boulos F,Jabbour MN,Chalala C,Boustany RM,Kadara H,Zhang Z,Zheng Y,Joyce B,Hou L,Bazarbachi A,Calin G,Nasr R

    更新日期：2017-12-04 00:00:00

  • Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution 31P NMR Coupled with Enzymatic Hydrolysis.

    abstract：:Solution Phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy coupled with enzymatic hydrolysis (EH) with commercially available phosphatases was used to characterize phosphorus (P) compounds in extracts of the dominant aquatic macrophytes and algae in a eutrophic lake. Total extractable organic P (Po) conc...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep37164

    authors： Feng W,Zhu Y,Wu F,He Z,Zhang C,Giesy JP

    更新日期：2016-11-16 00:00:00

  • Solvent-free bulk polymerization of lignin-polycaprolactone (PCL) copolymer and its thermoplastic characteristics.

    abstract：:The pristine lignin molecules contain multiple reactive hydroxyl [OH] groups, some of which undergo limited polymerization depending on their configuration (aromatic or aliphatic) or conformation. The key issue in lignin-polymerization is to quantify the number of hydroxyl groups in the pristine molecules for subseque...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43296-2

    authors： Park IK,Sun H,Kim SH,Kim Y,Kim GE,Lee Y,Kim T,Choi HR,Suhr J,Nam JD

    更新日期：2019-05-07 00:00:00

  • Piwi-like 1 and -2 protein expression levels are prognostic factors for muscle invasive urothelial bladder cancer patients.

    abstract：:Piwi-like proteins are essential for stem-cell maintenance and self-renewal in multicellular organisms. We analyzed the expression of Piwi-like 1 and Piwi-like 2 by immunohistochemistry (IHC) in 95 muscle invasive bladder cancer (MIBC) samples using tissue microarray. Application of an immunoreactive score (IRS) revea...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-35637-4

    authors： Eckstein M,Jung R,Weigelt K,Sikic D,Stöhr R,Geppert C,Agaimy A,Lieb V,Hartmann A,Wullich B,Wach S,Taubert H

    更新日期：2018-12-06 00:00:00

  • Chromatin Compaction by Small RNAs and the Nuclear RNAi Machinery in C. elegans.

    abstract：:DNA is organized and compacted into higher-order structures in order to fit within nuclei and to facilitate gene regulation. Mechanisms by which higher order chromatin structures are established and maintained are poorly understood. In C. elegans, nuclear-localized small RNAs engage the nuclear RNAi machinery to regul...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45052-y

    authors： Fields BD,Kennedy S

    更新日期：2019-06-21 00:00:00

  • Sporadic colon cancer murine models demonstrate the value of autoantibody detection for preclinical cancer diagnosis.

    abstract：:Although autoantibody detection has been proposed for diagnosis of colorectal cancer, little is known about their initial production and development correlation with cancer progression. Azoxymethane/dextran sodium sulfate (AOM/DSS)-treated mice developed colon adenocarcinoma in the distal colon similar to human sporad...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep02938

    authors： Barderas R,Villar-Vázquez R,Fernández-Aceñero MJ,Babel I,Peláez-García A,Torres S,Casal JI

    更新日期：2013-10-15 00:00:00

  • Hyphal network whole field imaging allows for accurate estimation of anastomosis rates and branching dynamics of the filamentous fungus Podospora anserina.

    abstract：:The success of filamentous fungi in colonizing most natural environments can be largely attributed to their ability to form an expanding interconnected network, the mycelium, or thallus, constituted by a collection of hyphal apexes in motion producing hyphae and subject to branching and fusion. In this work, we charac...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-57808-y

    authors： Dikec J,Olivier A,Bobée C,D'Angelo Y,Catellier R,David P,Filaine F,Herbert S,Lalanne C,Lalucque H,Monasse L,Rieu M,Ruprich-Robert G,Véber A,Chapeland-Leclerc F,Herbert E

    更新日期：2020-02-21 00:00:00

  • REAC technology modifies pathological neuroinflammation and motor behaviour in an Alzheimer's disease mouse model.

    abstract：:The search for new therapeutic approaches to Alzheimer disease (AD) is a major goal in medicine and society, also due to the impressive economic and social costs of this disease. In this scenario, biotechnologies play an important role. Here, it is demonstrated that the Radio Electric Asymmetric Conveyer (REAC), an in...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep35719

    authors： Lorenzini L,Giuliani A,Sivilia S,Baldassarro VA,Fernandez M,Lotti Margotti M,Giardino L,Fontani V,Rinaldi S,Calzà L

    更新日期：2016-10-24 00:00:00

  • Cholecystectomy is associated with higher risk of early recurrence and poorer survival after curative resection for early stage hepatocellular carcinoma.

    abstract：:Although cholecystectomy has been reported to be associated with increased risk of developing hepatocellular carcinoma (HCC), the association between cholecystectomy and prognosis of HCC patients underwent curative resection has never been examined. Through retrospective analysis of the data of 3933 patients underwent...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28229

    authors： Li T,Wang SK,Zhi XT,Zhou J,Dong ZR,Zhang ZL,Sun HC,Ye QH,Fan J

    更新日期：2016-06-20 00:00:00

  • Exploration of the relationship between biogas production and microbial community under high salinity conditions.

    abstract：:High salinity frequently causes inhibition and even failure in anaerobic digestion. To explore the impact of increasing NaCl concentrations on biogas production, and reveal the microbial community variations in response to high salinity stress, the Illumina high-throughput sequencing technology was employed. The resul...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-01298-y

    authors： Wang S,Hou X,Su H

    更新日期：2017-04-25 00:00:00

  • Crystal structure of the BoNT/A2 receptor-binding domain in complex with the luminal domain of its neuronal receptor SV2C.

    abstract：:A detailed molecular understanding of botulinum neurotoxin (BoNT)/host-cell-receptor interactions is fundamental both for developing strategies against botulism and for generating improved BoNT variants for medical applications. The X-ray crystal structure of the receptor-binding domain (HC) of BoNT/A1 in complex with...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep43588

    authors： Benoit RM,Schärer MA,Wieser MM,Li X,Frey D,Kammerer RA

    更新日期：2017-03-02 00:00:00

  • Multiple genotypes of Echovirus 11 circulated in mainland China between 1994 and 2017.

    abstract：:Echovirus 11 (E-11) is one of the most frequently isolated enteroviruses causing meningitis and other diseases such as hand, foot, and mouth disease (HFMD) and acute flaccid paralysis (AFP). Fifty-nine newly determined E-11 VP1 sequences from the China AFP and HFMD surveillance network and 500 E-11 VP1 sequences obtai...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46870-w

    authors： Li J,Yan D,Chen L,Zhang Y,Song Y,Zhu S,Ji T,Zhou W,Gan F,Wang X,Hong M,Guan L,Shi Y,Wu G,Xu W

    更新日期：2019-07-22 00:00:00

  • The global view of mRNA-related ceRNA cross-talks across cardiovascular diseases.

    abstract：:Competing endogenous RNA (ceRNA) have received wide attention because they are a novel way to regulate genes through sharing microRNAs (miRNAs) that are crucial for complex processes in many diseases. However, no systematic analysis of ceRNA mechanism in cardiovascular disease (CVD) is known. To gain insights into the...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-10547-z

    authors： Song C,Zhang J,Qi H,Feng C,Chen Y,Cao Y,Ba L,Ai B,Wang Q,Huang W,Li C,Sun H

    更新日期：2017-08-31 00:00:00

  • Genome scans for divergent selection in natural populations of the widespread hardwood species Eucalyptus grandis (Myrtaceae) using microsatellites.

    abstract：:Identification of loci or genes under natural selection is important for both understanding the genetic basis of local adaptation and practical applications, and genome scans provide a powerful means for such identification purposes. In this study, genome-wide simple sequence repeats markers (SSRs) were used to scan f...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep34941

    authors： Song Z,Zhang M,Li F,Weng Q,Zhou C,Li M,Li J,Huang H,Mo X,Gan S

    更新日期：2016-10-17 00:00:00

  • Comparative Mitochondrial Genome Analysis of Eligma narcissus and other Lepidopteran Insects Reveals Conserved Mitochondrial Genome Organization and Phylogenetic Relationships.

    abstract：:In this study, we sequenced the complete mitochondrial genome of Eligma narcissus and compared it with 18 other lepidopteran species. The mitochondrial genome (mitogenome) was a circular molecule of 15,376 bp containing 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and an...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep26387

    authors： Dai LS,Zhu BJ,Zhao Y,Zhang CF,Liu CL

    更新日期：2016-05-25 00:00:00

  • Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis.

    abstract：:Lignosus rhinocerotis (Tiger milk mushroom) is an important folk medicine for indigenous peoples in Southeast Asia. We previously reported its de novo assembled 34.3 Mb genome encoding a repertoire of proteins including a putative bioactive fungal immunomodulatory protein. Here we report the cDNA of this new member (F...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep30010

    authors： Pushparajah V,Fatima A,Chong CH,Gambule TZ,Chan CJ,Ng ST,Tan CS,Fung SY,Lee SS,Tan NH,Lim RL

    更新日期：2016-07-27 00:00:00

  • Severe Fever with Thrombocytopenia Syndrome Phlebovirus causes lethal viral hemorrhagic fever in cats.

    abstract：:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever caused by the SFTS phlebovirus (SFTSV). SFTS patients were first reported in China, followed by Japan and South Korea. In 2017, cats were diagnosed with SFTS for the first time, suggesting that these animals are susceptible to SFTSV. T...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48317-8

    authors： Park ES,Shimojima M,Nagata N,Ami Y,Yoshikawa T,Iwata-Yoshikawa N,Fukushi S,Watanabe S,Kurosu T,Kataoka M,Okutani A,Kimura M,Imaoka K,Hanaki K,Suzuki T,Hasegawa H,Saijo M,Maeda K,Morikawa S

    更新日期：2019-08-19 00:00:00

  • Minimal domain of bacterial phytochrome required for chromophore binding and fluorescence.

    abstract：:Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of near-infrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engineered R. palustris...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18348

    authors： Rumyantsev KA,Shcherbakova DM,Zakharova NI,Emelyanov AV,Turoverov KK,Verkhusha VV

    更新日期：2015-12-18 00:00:00

  • Deciphering host lysosome-mediated elimination of Plasmodium berghei liver stage parasites.

    abstract：:Liver stage Plasmodium parasites reside in a parasitophorous vacuole (PV) that associates with lysosomes. It has previously been shown that these organelles can have beneficial as well as harmful effects on the parasite. Yet it is not clear how the association of lysosomes with the parasite is controlled and how inter...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-44449-z

    authors： Niklaus L,Agop-Nersesian C,Schmuckli-Maurer J,Wacker R,Grünig V,Heussler VT

    更新日期：2019-05-28 00:00:00

  • Inhibition of Influenza A Virus Infection by Fucoidan Targeting Viral Neuraminidase and Cellular EGFR Pathway.

    abstract：:Development of novel anti-influenza A virus (IAV) drugs with high efficiency and low toxicity is critical for preparedness against influenza outbreaks. Herein, we investigated the anti-IAV activities and mechanisms of fucoidan in vitro and in vivo. The results showed that a fucoidan KW derived from brown algae Kjellma...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep40760

    authors： Wang W,Wu J,Zhang X,Hao C,Zhao X,Jiao G,Shan X,Tai W,Yu G

    更新日期：2017-01-17 00:00:00

  • Persistent current in 2D topological superconductors.

    abstract：:A junction between two boundaries of a topological superconductor (TSC), mediated by localized edge modes of Majorana fermions, is investigated. The tunneling of fermions across the junction depends on the magnetic flux. It breaks the time-reversal symmetry at the boundary of the sample. The persistent current is dete...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07492-2

    authors： Karnaukhov IN

    更新日期：2017-08-02 00:00:00

  • Decision-making factors for best supportive care alone and prognostic factors after best supportive care in non-small cell lung cancer patients.

    abstract：:Among patients with non-small cell lung cancer (NSCLC), best supportive care (BSC) is well-known to improve patient's quality of life and prolong survival. This study aimed to clarify (1) the decision-making factors of BSC alone and (2) the prognostic factors after selection of no further anticancer therapies. We retr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56431-w

    authors： Kitazawa H,Takeda Y,Naka G,Sugiyama H

    更新日期：2019-12-27 00:00:00

  • Inducible orthogonal aminoacylation demonstrates that charging is required for mitochondrial tRNA import in Trypanosoma brucei.

    abstract：:Orthogonal aminoacyl-tRNA synthetase/tRNA pairs have emerged as powerful means of site-specifically introducing non-standard amino acids into proteins in vivo. Using amino acids with crosslinking moieties this method allows the identification of transient protein-protein interactions. Here we have introduced a previou...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-47268-4

    authors： Huot JL,Shikha S,Schneider A

    更新日期：2019-07-25 00:00:00

  • Thymoquinone Alleviates the Experimental Diabetic Peripheral Neuropathy by Modulation of Inflammation.

    abstract：:Thymoquinone has been reported to exhibit antioxidant and anti-inflammatory effects. Inflammation plays an important role in pathogenesis of diabetic peripheral neuropathy. This study investigated the effects of TQ on proliferation and apoptosis of Schwann cells exposed to high glucose conditions and electrophysiologi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31656

    authors： Chen L,Li B,Chen B,Shao Y,Luo Q,Shi X,Chen Y

    更新日期：2016-08-22 00:00:00

  • G6Pase location in the endoplasmic reticulum: Implications on compartmental analysis of FDG uptake in cancer cells.

    abstract：:The favourable kinetics of 18F-fluoro-2-deoxyglucose (FDG) permits to depict cancer glucose consumption by a single evaluation of late tracer uptake. This standard procedure relies on the slow radioactivity loss, usually attributed to the limited tumour expression of G6P-phosphatase (G6Pase). However, this classical i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-38973-1

    authors： Scussolini M,Bauckneht M,Cossu V,Bruno S,Orengo AM,Piccioli P,Capitanio S,Yosifov N,Ravera S,Morbelli S,Piana M,Sambuceti G,Caviglia G,Marini C

    更新日期：2019-02-26 00:00:00

  • All framing effects are not created equal: Low convergent validity between two classic measurements of framing.

    abstract：:Human risk-taking attitudes can be influenced by two logically equivalent but descriptively different frames, termed the framing effect. The classic hypothetical vignette-based task (Asian disease problem) and a recently developed reward-based gambling task have been widely used to assess individual differences in the...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep30071

    authors： Zhen S,Yu R

    更新日期：2016-07-20 00:00:00

  • Mediating role of attenuated physiological arousal on the association between psychopathic traits and fairness norm violation.

    abstract：:The low-fear model of primary psychopathy has been supported by empirical findings such as attenuated physiological arousal in anticipation of threatening stimuli. The somatic marker hypothesis proposes that salient changes in the bodily state are processed as signals of whether a situation is good or bad and guide an...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-54676-z

    authors： Osumi T

    更新日期：2019-12-02 00:00:00

  • Three-dimensional Fe2O3 nanocubes/nitrogen-doped graphene aerogels: nucleation mechanism and lithium storage properties.

    abstract：:We developed a solvothermal-induced self-assembly approach to construct three dimensional (3D) macroscopic Fe2O3 nanocubes/nitrogen-doped graphene (Fe2O3-NC/GN) aerogel as anode materials for lithium-ion batteries (LIBs). The Fe2O3 nanocubes with length of ~50 nm are homogeneously anchored on 3D GN frameworks and as s...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07171

    authors： Wang R,Xu C,Sun J,Gao L

    更新日期：2014-11-25 00:00:00