Abstract:
:Peripheral blood and/or bone marrow lymphoblasts from 25 patients with acute lymphoblastic leukemia (ALL) were tested with a panel of monoclonal antibodies (MoAbs) and conventional hematopoietic markers by three different laboratories. The results were analysed to evaluate the reproducibility of ALL phenotype determinations. Specimens were transported between laboratories by 24-h courier service and were classified on the basis of indirect immunofluorescence MoAb reactivities as follows: B-lineage ALL (BA-1+T-MCS-2-); T-lineage ALL (T+BA-1-MCS-2-); myeloid antigen ALL (MCS-2+BA-1-CALLA-T-) and unclassified ALL (BA-1-MCS-2-CALLA-T-). Conventional marker studies for surface immunoglobulin (sIg), cytoplasmic immunoglobulin (cIg), sheep erythrocyte rosette formation (E) and nuclear terminal deoxynucleotidyl transferase (TdT) were also performed. In the cases with sufficient marker data to permit classification, 90% (18/20) were identically classified by different laboratories and this concordance was statistically significant (p less than 0.05). The agreement between laboratories for individual MoAb and conventional marker analyses was statistically significant (p less than 0.05) for all markers with the exception of BA-2, cIg and TdT determinations. Six of 7 discordant BA-2 cases represented BA-2+ evaluations which had subsequent BA-2- results following specimen transportation. These findings suggest instability of the BA-2 antigen to transport conditions. A similar pattern of positive to negative evaluations following transportation was observed in 5/5 discordant results involving other MoAbs. Disagreement between laboratories for cIg and TdT determinations implies that the detection of cytoplasmic or nuclear antigens may be more prone to subjective interpretation than cell surface antigen marker analyses. Our findings suggest that immunofluorescence marker studies employing MoAbs to cell surface antigens are in general highly reproducible. Our results also indicate that specimen storage conditions and the cellular location of target antigens are important variables which may affect the reproducibility of ALL phenotype determinations.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Sobol RE,Mick R,LeBien TW,Ozer H,Minowada J,Anderson K,Ellison RR,Cuttner J,Morrison A,Richards F 2nddoi
10.1016/0145-2126(86)90083-4subject
Has Abstract,Author List Incompletepub_date
1986-01-01 00:00:00pages
481-5issue
5eissn
0145-2126issn
1873-5835journal_volume
10pub_type
杂志文章abstract::A primary mitochondrial defect may be pivotal in the pathogenesis of acquired idiopathic sideroblastic anemia (AISA). The mitochondrial respiratory chain is involved in mitochondrial iron uptake and supply of ferrous iron (Fe2+) for heme synthesis. Mitochondrial DNA (mtDNA) comes into play because several subunits of ...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/s0145-2126(99)00160-5
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abstract::MicroRNAs (miRNAs) are small endogenous RNA molecules that regulate gene expression at the post-transcriptional level through its sequence complementation with target mRNAs. An individual miRNA species can simultaneously influence the expression of multiple genes and conversely, several miRNAs can synchronously contro...
journal_title:Leukemia research
pub_type: 杂志文章,评审
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journal_title:Leukemia research
pub_type: 总体
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更新日期:2008-09-01 00:00:00
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journal_title:Leukemia research
pub_type: 信件
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2004.10.004
更新日期:2005-04-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.05.001
更新日期:2007-12-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.05.024
更新日期:2010-11-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.leukres.2014.02.003
更新日期:2014-05-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.05.016
更新日期:2006-01-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90181-0
更新日期:1987-01-01 00:00:00
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journal_title:Leukemia research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.leukres.2006.10.014
更新日期:2007-04-01 00:00:00
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journal_title:Leukemia research
pub_type: 历史文章,杂志文章
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更新日期:2016-12-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00137-9
更新日期:1996-02-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.11.008
更新日期:2010-08-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2019.02.009
更新日期:2019-04-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.01.004
更新日期:2006-06-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90108-1
更新日期:1987-01-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(99)00033-8
更新日期:1999-07-01 00:00:00
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pub_type: 临床试验,杂志文章
doi:10.1016/j.leukres.2008.05.015
更新日期:2008-12-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2003.10.022
更新日期:2004-06-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.08.009
更新日期:2009-04-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2016.09.008
更新日期:2016-10-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.07.026
更新日期:2009-01-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(02)00087-5
更新日期:2003-02-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(82)90010-8
更新日期:1982-01-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(88)90097-5
更新日期:1988-01-01 00:00:00
abstract::Aberrant promoter methylation may contribute to the hematopoietic disturbances in myelodysplastic syndromes (MDS). To explore a possible mechanism, we therefore analyzed expression of DNA methyltransferase (DNMT) subtypes kinetics and aberrant promoter methylation of key regulatory genes during MDS hematopoiesis. An i...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.08.014
更新日期:2009-03-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.07.022
更新日期:2010-04-01 00:00:00