Abstract:
OBJECTIVES:To compare the performance of two established androgen receptor splice variant 7 (AR-V7) mRNA detection systems, as paradoxical responses to next-generation androgen-deprivation therapy in AR-V7 mRNA-positive circulating tumour cells (CTC) of patients with castration-resistant prostate cancer (CRPC) could be related to false-positive classification using detection systems with different sensitivities. MATERIALS AND METHODS:We compared the performance of two established mRNA-based AR-V7 detection technologies using either SYBR Green or TaqMan chemistries. We assessed in vitro performance using eight genitourinary cancer cell lines and serial dilutions in three AR-V7-positive prostate cancer cell lines, as well as in 32 blood samples from patients with CRPC. RESULTS:Both assays performed identically in the cell lines and serial dilutions showed identical diagnostic thresholds. Performance comparison in 32 clinical patient samples showed perfect concordance between the assays. In particular, both assays determined AR-V7 mRNA-positive CTCs in three patients with unexpected responses to next-generation anti-androgen therapy. Thus, technical differences between the assays can be excluded as the underlying reason for the unexpected responses to next-generation anti-androgen therapy in a subset of AR-V7 patients. CONCLUSIONS:Irrespective of the method used, patients with AR-V7 mRNA-positive CRPC should not be systematically precluded from an otherwise safe treatment option.
journal_name
BJU Intjournal_title
BJU internationalauthors
Bernemann C,Steinestel J,Humberg V,Bögemann M,Schrader AJ,Lennerz JKdoi
10.1111/bju.14146subject
Has Abstractpub_date
2018-08-01 00:00:00pages
219-226issue
2eissn
1464-4096issn
1464-410Xjournal_volume
122pub_type
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pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2004-10-01 00:00:00
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更新日期:2008-11-01 00:00:00
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更新日期:2006-01-01 00:00:00
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更新日期:2000-10-01 00:00:00