Abstract:
AIM:To investigate the effect of epigallocatechin gallate (EGCG) on structural changes of gut microbiota in colorectal carcinogenesis. METHODS:An azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis mouse model was established. Forty-two female FVB/N mice were randomly divided into the following three groups: group 1 (10 mice, negative control) was treated with vehicle, group 2 (16 mice, positive control) was treated with AOM plus vehicle, and group 3 (16 mice, EG) was treated with AOM plus EGCG. For aberrant crypt foci (ACF) evaluation, the colons were rapidly took out after sacrifice, rinsed with saline, opened longitudinally, laid flat on a polystyrene board, and fixed with 10% buffered formaldehyde solution before being stained with 0.2% methylene blue in saline. For tumor evaluation, the colon was macroscopically inspected and photographed, then the total number of tumors was enumerated and tumor size measured. For histological examination, the fixed tissues were paraffin-embedded and sectioned at 5 mm thickness. Microbial genomic DNA was extracted from fecal and intestinal content samples using a commercial kit. The V4 hypervariable regions of 16S rRNA were PCR-amplified with the barcoded fusion primers. Using the best hit classification option, the sequences from each sample were aligned to the RDP 16S rRNA training set to classify the taxonomic abundance in QIIME. Statistical analyses were then performed. RESULTS:Treatment of mice with 1% EGCG caused a significant decrease in the mean number of ACF per mouse, when compared with the model mice treated with AOM/DSS (5.38 ± 4.24 vs 13.13 ± 3.02, P < 0.01). Compared with the positive control group, 1% EGCG treatment dependently decreased tumor load per mouse by 85% (33.96 ± 6.10 vs 2.96 ± 2.86, respectively, P < 0.01). All revealed that EGCG could inhibit colon carcinogenesis by decreasing the number of precancerous lesions as well as solid tumors, with reduced tumor load and delayed histological progression of CRC. During the cancerization, the diversity of gut microbiota increased, potential carcinogenic bacteria such as Bacteroides were enriched, and the abundance of butyrate-producing bacteria (Clostridiaceae, Ruminococcus, etc.) decreased continuously. In contrast, the structure of gut microbiota was relatively stable during the intervention of EGCG on colon carcinogenesis. Enrichment of probiotics (Bifidobacterium, Lactobacillu, etc.) might be a potential mechanism for EGCG's effects on tumor suppression. Via bioinformatics analysis, principal coordinate analysis and cluster analysis of the tumor formation process, we found that the diversity of gut microbiota increased in the tumor model group while that in the EGCG interfered group (EG) remained relatively stable. CONCLUSION:Gut microbiota imbalance might be a potential mechanism for the prevention of malignant transformation by EGCG, which is significant for diagnosis, treatment, prognosis evaluation, and prevention of colorectal cancer.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Wang X,Ye T,Chen WJ,Lv Y,Hao Z,Chen J,Zhao JY,Wang HP,Cai YKdoi
10.3748/wjg.v23.i46.8128subject
Has Abstractpub_date
2017-12-14 00:00:00pages
8128-8139issue
46eissn
1007-9327issn
2219-2840journal_volume
23pub_type
杂志文章abstract::Irritable bowel syndrome (IBS) is a highly prevalent functional disorder that reduces patients' quality of life. It is a chronic disorder characterized by abdominal pain or discomfort associated with disordered defecation in the absence of identifiable structural or biochemical abnormalities. IBS imposes a significant...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i27.8867
更新日期:2014-07-21 00:00:00
abstract:AIM:To compare the number of regulatory T-cells (Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR (qPCR) method in patients suffering from inflammatory bowel disease (IBD). METHODS:Tregs percentages obtained by both flow cytometry and qPCR methods in 35 adult IBD patients, 18 ou...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i11.3325
更新日期:2015-03-21 00:00:00
abstract:AIM:To investigate the effects of osteopontin (OPN) gene expression knockdown on colon cancer Lovo cells in vitro. METHODS:Four candidate small interfering RNA (siRNA) constructs targeting the OPN gene and a scrambled control sequence (NC-siRNA) were synthesized and inserted into a pGPU6/GFP/Neo expression vector. Aft...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i30.10440
更新日期:2014-08-14 00:00:00
abstract:AIM:To identify predictors for synchronous liver metastasis from resectable pancreatic ductal adenocarcinoma (PDAC) and assess unresectability of synchronous liver metastasis. METHODS:Retrospective records of PDAC patients with synchronous liver metastasis who underwent simultaneous resections of primary PDAC and sync...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v22.i45.10024
更新日期:2016-12-07 00:00:00
abstract:BACKGROUND:Liver resection is an effective treatment for benign and malignant liver tumors. However, a method for preoperative evaluation of hepatic reserve has not yet been established. Previously reported assessments of preoperative hepatic reserve focused only on liver failure in the early postoperative period and d...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i7.725
更新日期:2020-02-21 00:00:00
abstract::Recently, the use of confocal laser endomicroscopy (CLE) in the diagnosis of chronic ulcerative colitis (CUC) was reported. In this brief report we aimed to assess the application of probe-based CLE to characterize colonic mucosa and dysplasia in CUC. The study involved a patient presenting long-standing CUC. Confocal...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v17.i5.677
更新日期:2011-02-07 00:00:00
abstract::Repeated surveys from Europe, the United States, Australia, and New Zealand have shown that adherence to an evidence-based perioperative care protocol, such as Enhanced Recovery After Surgery (ERAS), has been generally low. It is of great importance to support the implementation of the ERAS protocol as it has been sho...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i44.16615
更新日期:2014-11-28 00:00:00
abstract:AIM:To evaluate the influence of multiple samplings during esophagogastroduodenoscopy (EGD) on the accuracy of the rapid urease test, and the validity of newly developed rapid urease tests, HelicotecUT plus test and HelicotecUT test, CLO test and ProntoDry test. METHODS:A total of 355 patients undergoing EGD for dyspe...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i23.2926
更新日期:2010-06-21 00:00:00
abstract:AIM:To detect the expression of mitochondrial uncoupling protein 2 (UCP2) in colon cancer and analyze the relation between UCP2 expression and clinical pathological features of colon cancer. METHODS:Fifteen colon tissue samples and 15 its adjacent tissue samples were obtained from colon cancer patients during surgical...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i45.5773
更新日期:2010-12-07 00:00:00
abstract::Hyperbilirubinemia has been presumed to prevent the process of atherogenesis and cancerogenesis mainly by decreasing oxidative stress. Dubin-Johnson syndrome is a rare, autosomal recessive, inherited disorder characterized by biphasic, predominantly conjugated hyperbilirubinemia with no progression to end-stage liver ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i6.946
更新日期:2013-02-14 00:00:00
abstract:AIM:To determine whether local antibiotic resistance involves P-glycoprotein (Pgp)-mediated active drug out-pumping during Helicobacter pylori (H pylori) infection treatment with classic antibiotic therapy. METHODS:Pgp activity was determined in gastric mucosa biopsy specimens obtained from 53 patients with pathohisto...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i18.2720
更新日期:2005-05-14 00:00:00
abstract:AIM:To assess the role of IgM and IgG immunohistochemistry (IHC) in the evaluation of autoimmune liver conditions--autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), and primary sclerosing cholangitis (PSC). METHODS:Forty one biopsies from untreated patients diagnosed with autoimmune liver disease (AIH, n = ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i4.453
更新日期:2010-01-28 00:00:00
abstract:AIM:To investigate the prognostic value of vascular endothelial growth factor messenger RNA (VEGF mRNA) in the peripheral blood (PB) of patients with hepatocellular carcinoma (HCC) undergoing curative resection. METHODS:Using a reverse-transcription polymerase chain reaction (RT-PCR)-based assay, VEGF mRNA in the PB w...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v10.i5.643
更新日期:2004-03-01 00:00:00
abstract:AIM:To test the Genval recommendations and the usefulness of a short trial of proton pump inhibitor (PPI) in the initial management and maintenance treatment of gastroesophageal reflux disease (GERD) patients. METHODS:Five hundred and seventy seven patients with heartburn were recruited. After completing a psychometri...
journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3748/wjg.v17.i9.1160
更新日期:2011-03-07 00:00:00
abstract:AIM:To determine the role of NOB1, a regulator of cell survival in yeast, in human colorectal cancer cells. METHODS:Lentivirus-mediated small interfering RNA (siRNA) was used to inhibit NOB1 expression in RKO human colorectal cancer cells in vitro and in vivo in a mouse xenograft model. The in vitro and in vivo knockd...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i3.868
更新日期:2015-01-21 00:00:00
abstract:AIM:To construct pcDNA3.1-Egr.1p-p16 recombinant plasmid and investigate the expression of p16 in pancreatic cancer JF305 cells induced by radiation and the feasibility of gene radiotherapy for pancreatic carcinoma. METHODS:Human p16 cDNA was ligated to the downstream of Egr-1 promotor to construct pcDNA3.1-Egr.1p-p16...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i31.4214
更新日期:2007-08-21 00:00:00
abstract:AIM:To establish the prognosis and feasibility of en-bloc vascular resection of stage II pancreatic adenocarcinoma of the head and uncinate process. METHODS:We retrospectively analyzed 87 patients with stage II pancreatic adenocarcinoma, who were subjected to pancreaticoduodenectomy (PD) and pylorus-preserving PD (PPP...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i8.997
更新日期:2010-02-28 00:00:00
abstract::Enterolithiasis or formation of gastrointestinal concretions is an uncommon medical condition that develops in the setting of intestinal stasis in the presence of the intestinal diverticula, surgical enteroanastomoses, blind pouches, afferent loops, incarcerated hernias, small intestinal tumors, intestinal kinking fro...
journal_title:World journal of gastroenterology
pub_type: 历史文章,杂志文章,评审
doi:10.3748/wjg.v20.i47.17819
更新日期:2014-12-21 00:00:00
abstract:AIM:To investigate the risk factors of colon cancer and rectal cancer. METHODS:A nested case-control study was conducted in a cohort of 64 693 subjects who participated in a colorectal cancer screening program from 1989 to 1998 in Jiashan county, Zhejiang, China. 196 cases of colorectal cancer were detected from 1990 ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i1.99
更新日期:2003-01-01 00:00:00
abstract:AIM:To evaluate the diagnostic value and safety mainly regarding incidents of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) for pancreatic cystic lesions (PCLs). METHODS:A total of 150 consecutive patients with suspected PCLs were prospectively enrolled from April 2015 to November 2016. We finally enro...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v23.i30.5610
更新日期:2017-08-14 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common primary cancers in the world. Surgery is the gold standard for treatment of patients with HCC. Recurrence and metastasis are the major obstacles to further improve the prognosis of HCC. Most recurrences are intrahepatic. However, 30% of the recurrences are extra...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i40.5413
更新日期:2007-10-28 00:00:00
abstract:AIM:To make a retrospective analysis of endoscopy findings and clinicopathologic characteristics of colonic schistosomiasis in order to further improve our understanding of the disease and decrease its misdiagnosis. METHODS:Endoscopy findings and clinicopathologic characteristics of 46 intestinal schistosomiasis patie...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i6.723
更新日期:2010-02-14 00:00:00
abstract::Enzymatic metabolism of the 20C polyunsaturated fatty acid (PUFA) arachidonic acid (AA) occurs via the cyclooxygenase (COX) and lipoxygenase (LOX) pathways, and leads to the production of various bioactive lipids termed eicosanoids. These eicosanoids have a variety of functions, including stimulation of homeostatic re...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v21.i41.11748
更新日期:2015-11-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章
doi:10.3748/wjg.v10.i15.2272
更新日期:2004-08-01 00:00:00
abstract:AIM:To evaluate tumor necrosis factor-α converting enzyme (TACE) methylation status in patients with chronic hepatitis B (CHB). METHODS:Eighty patients with hepatitis B e antigen (HBeAg)-positive CHB, 80 with HBeAg-negative CHB, and 40 healthy controls (HCs) were randomly enrolled in this study. Genomic DNA was extrac...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i27.8382
更新日期:2015-07-21 00:00:00
abstract:AIM:To explore whether the presence of a sliding hiatus hernia influences gastroesophageal reflux. METHODS:Endoscopy and 24 h pH monitoring were performed for 197 outpatients with gastroesophageal reflux symptoms. RESULTS:Of the 197 patients with symptoms of gastroesophageal reflux, patients with hiatus hernia accoun...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v3.i1.27
更新日期:1997-03-15 00:00:00
abstract:AIM:To investigate the effect of side-stream smoking on gut microflora composition, intestinal inflammation and expression of tight junction proteins. METHODS:C57BL/6 mice were exposed to side-stream cigarette smoking for one hour daily over eight weeks. Cecal contents were collected for microbial composition analysis...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i18.2180
更新日期:2012-05-14 00:00:00
abstract:AIM:To investigate the relationship between 579 G>T polymorphisms in the DNMT3B gene, which is involved in de novo methylation and associated with the risk of esophagus cancer (EC) in Chinese. METHODS:DNMT3B 579 G>T genotypes were determined by PCR-RFLP in 194 EC patients and 210 healthy controls matched for age and s...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.2230
更新日期:2008-04-14 00:00:00
abstract:AIM:gamma-glutamyl transpeptidase (GGT) has been reported as a virulence and colonizing factor of Helicobacter pylori (H pylori). This study examined the effect of GGT on the growth of H pylori. METHODS:Standard H pylori strain NCTC 11637 and 4 clinical isolates with different levels of GGT activity as measured by an ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v10.i20.2994
更新日期:2004-10-15 00:00:00
abstract::Oncological treatment is currently directed toward a tailored therapy concept. Squamous cell carcinoma of the anal canal could be considered a suitable platform to test new therapeutic strategies to minimize treatment morbidity. Standard of care for patients with anal canal cancer consists of a combination of radiothe...
journal_title:World journal of gastroenterology
pub_type: 社论,评审
doi:10.3748/wjg.v18.i44.6349
更新日期:2012-11-28 00:00:00