Prominent role of gamma-glutamyl-transpeptidase on the growth of Helicobacter pylori.

Abstract:

AIM:gamma-glutamyl transpeptidase (GGT) has been reported as a virulence and colonizing factor of Helicobacter pylori (H pylori). This study examined the effect of GGT on the growth of H pylori. METHODS:Standard H pylori strain NCTC 11637 and 4 clinical isolates with different levels of GGT activity as measured by an enzymatic assay were used in this study. Growth inhibition and stimulation studies were carried out by culturing H pylori in brain heart infusion broth supplemented with specific GGT inhibitor (L-serine sodium borate complex, SBC) or enhancer (glutathione together with glycyl-glycine), respectively. The growth profiles of H pylori were determined based on viable bacterial count at time interval. RESULTS:Growth was more profuse for H pylori isolates with higher GGT activity than those present with lower GGT activity. However, in the presence of SBC, growth of H pylori was retarded in a dose dependent manner (P = 0.034). In contrast, higher growth rate was observed when GGT activity was enhanced in the presence of glutathione and glycyl-glycine. CONCLUSION:Higher GGT activity provides an advantage to the growth of H pylori in vitro. Inhibition of GGT activity by SBC resulted in growth retardation. The study shows that GGT plays an important role on the growth of H pylori.

journal_name

World J Gastroenterol

authors

Gong M,Ho B

doi

10.3748/wjg.v10.i20.2994

keywords:

subject

Has Abstract

pub_date

2004-10-15 00:00:00

pages

2994-6

issue

20

eissn

1007-9327

issn

2219-2840

journal_volume

10

pub_type

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