Self-assembly of nucleopeptides to interact with DNAs.

Abstract:

:As a novel class of biomaterials, nucleopeptides, via the conjugation of nucleobases and peptides, usually self-assemble to form nanofibres driven mainly by hydrogen bonds. Containing nucleobase(s), nucleopeptides have a unique property-interacting with nucleic acids. Here we report the design and characterization of nucleopeptides that self-assemble in water and are able to interact with single-stranded DNAs (ssDNAs). Containing nucleobases on their side chains, these nucleopeptides bind with the ssDNAs, and the ssDNAs reciprocally affect the self-assembly of nucleopeptides. In addition, the interactions between nucleopeptides and ssDNAs also decrease their proteolytic resistance against proteinase K, which further demonstrates the binding with ssDNAs. The nucleopeptides also interact with plasmid DNA and deliver hairpin DNA into cells. This work illustrates a new and rational approach to create soft biomaterials by the integration of nucleobases and peptides to bind with DNA, which may lead to the development of nucleopeptides for controlling DNA in cells.

journal_name

Interface Focus

journal_title

Interface focus

authors

Du X,Zhou J,Li X,Xu B

doi

10.1098/rsfs.2016.0116

subject

Has Abstract

pub_date

2017-12-06 00:00:00

pages

20160116

issue

6

eissn

2042-8898

issn

2042-8901

pii

rsfs20160116

journal_volume

7

pub_type

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