Abstract:
PURPOSE:Conventional laboratory detection methods for gastrointestinal parasites are time consuming, require considerable technical expertise and may suffer from poor analytical sensitivity. This study sought to evaluate the automated BD MAX Enteric Parasite Panel (EPP) for the detection of Cryptosporidium parvum/hominis, Entamoeba histolytica and Giardia duodenalis.Methodolgy. A total of 104 known positive samples (43 Cryptosporidium parvum/hominis and 61 G. duodenalis), 15 simulated samples (E. histolytica and other Entamoeba species) and 745 patient stool samples, submitted for enteric pathogen culture and microscopy, were inoculated into BD MAX EPP sample buffer tubes (SBTs). All specimens were blinded and tested within 7 days of SBT inoculation using the BD MAX EPP assay with results compared to those generated by microscopy.Results/Key findings. Combining the results from the known positive samples and anonymously tested patient samples, the sensitivity of the BD MAX EPP assay was 100 % for both Cryptosporidium spp. and G. duodenalis. Specificities of 99.7 and 98.9 % were calculated for the detection of Cryptosporidium spp. and G. duodenalis respectively. Insufficient clinical specimen data was available to determine the performance of the assay for E. histolytica detection. CONCLUSIONS:The findings of this study indicate that the BD MAX EPP is suitable for the detection of Cryptosporidium parvum/hominis and G. duodenalis from clinical specimens with reduced hands-on time and complexity compared to microscopy. Results for the detection of E. histolytica were promising although further work is required to evaluate the assay for the detection of this pathogen.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Perry MD,Corden SA,Lewis White Pdoi
10.1099/jmm.0.000558subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1118-1123issue
8eissn
0022-2615issn
1473-5644journal_volume
66pub_type
杂志文章abstract::Campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. Despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of C. fetus subsp. fetus on the male factor. Our objective was to examine the influence o...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.2008/001057-0
更新日期:2008-11-01 00:00:00
abstract::Sixty-one isolates of Candida albicans were tested for their phospholipase activity and this parameter was correlated with pathogenicity in albino mice. Of the isolates tested, 57 (93%) showed appreciable phospholipase activity and of these, 55 (90%) were pathogenic to mice. A significant correlation was found between...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-2-99
更新日期:1998-02-01 00:00:00
abstract::A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-4-336
更新日期:2002-04-01 00:00:00
abstract::Fifty clinical strains of Chlamydia trachomatis were studied by denaturing gradient gel electrophoresis (DGGE) of bacterial DNA amplified by the polymerase chain reaction (PCR). The strains belonged to the three most commonly encountered serovars in developed countries--D, E and F. Six reference strains, including the...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-43-1-14
更新日期:1995-07-01 00:00:00
abstract::Aminoglycoside-resistant variants of Pseudomonas aeruginosa strain PAO1 were readily selected by culturing the organism in medium containing increasing concentrations of gentamicin, tobramycin or amikacin until the strains were growing in a concentration of drug 128-fold greater than the minimal inhibitory concentrati...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-29-1-41
更新日期:1989-05-01 00:00:00
abstract::Two carbapenem-non-susceptible Klebsiella pneumoniae isolates, Z2554 and Z2110, were collected from a hospital in China and analysed by PFGE. K. pneumoniae Z2554 and Z2110 were genetically unrelated and showed resistance to ertapenem, and reduced susceptibility to imipenem and meropenem. Analysis of their beta-lactama...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.008094-0
更新日期:2009-09-01 00:00:00
abstract::Recent studies have shown that Providencia alcalifaciens is a diarrhoeal pathogen. It may cause diarrhoea by an invasive mechanism, as it invades cultured mammalian cells in vitro and intestinal epithelial cells of experimentally inoculated rabbits in vivo. To locate the gene(s) involved in invasion, TnphoA mutants of...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-8-682
更新日期:2002-08-01 00:00:00
abstract::A collection of 198 clinical isolates of strains belonging to the tribe Proteeae was examined for haemolytic activity on blood agar and in Brain Heart Infusion Broth. The strains were of diverse bacteriocin and O-antigenic types and from a wide variety of sources. They included representatives of all species of Morgan...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-25-1-17
更新日期:1988-01-01 00:00:00
abstract::A collection of 820 isolates of streptococci and aerococci from human blood or internal organs was classified by means of a set of cultural and biochemical tests. Of these, 719 (712 streptococci and 7 aerococci) were from patients believed to be suffering from a systemic infection: endocarditis 317; a purulent lesion ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-9-3-275
更新日期:1976-08-01 00:00:00
abstract::Transfer of an Hly plasmid determining production of alpha haemolysin to a non-haemolytic strain of Escherichia coli increased the virulence of the strain for mice. Injections of non-toxic amounts of alpha haemolysin, phenylhydrazine, haemoglobin, iron or manganese salts simulated the effect of the Hly plasmid by stim...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-15-1-23
更新日期:1982-02-01 00:00:00
abstract::In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000352
更新日期:2016-11-01 00:00:00
abstract::In this study, we evaluated the Sofia Streptococcus pneumoniae FIA test (Quidel Corporation, San Diego, CA, USA), a new immunofluorescence-based lateral flow test for the qualitative detection of S. pneumoniae antigen in urine or cerebrospinal fluid specimens. The analyses of 100 non-concentrated urine samples (includ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000877
更新日期:2018-12-01 00:00:00
abstract::A serial study of rotavirus infection in gnotobiotic piglets is described. They were infected when 7 days old and the course of infection was followed for 21 days, by immunofluorescence and histological examinations of the intestinal epithelium and by titration of the virus content of the gut lumen. There appeared to ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-11-3-325
更新日期:1978-08-01 00:00:00
abstract::The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000547
更新日期:2017-08-01 00:00:00
abstract::Serum opacity factor of Streptococcus suis (OFS) has recently been identified as a virulence determinant of an S. suis strain. In this study, we investigated the prevalence and variations of the ofs gene among 108 S. suis isolates from diseased and healthy pigs, and human patients. PCR screening and sequencing analysi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47755-0
更新日期:2008-04-01 00:00:00
abstract::The move towards pathology networks and hub-and-spoke models of medical laboratory service provision has significantly changed the flow of samples, and the impact of results on patients, over recent years. At the same time advances in technology, including rapid, simple to use molecular platforms, are changing the way...
journal_title:Journal of medical microbiology
pub_type: 信件
doi:10.1099/jmm.0.000919
更新日期:2019-03-01 00:00:00
abstract::SDS-PAGE and iso-enzyme analysis of 11 human isolates of Blastocystis hominis revealed at least two variants with different polypeptide patterns and two zymodemes, respectively. This is the first iso-enzyme and the second protein analysis to indicate strain differences in B. hominis. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-42-4-304
更新日期:1995-04-01 00:00:00
abstract::Resistance against vaginal colonisation by Mycoplasma pulmonis in strain TO mice after exposure to the mycoplasma was investigated. Eighteen mice from which M. pulmonis had been eliminated from the vagina, either naturally or by antibiotic therapy, were resistant to vaginal recolonisation. Specific antibody was measur...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-40-3-197
更新日期:1994-03-01 00:00:00
abstract::The susceptibilities of Actinobacillus actinomycetemcomitans cultures, grown as 1- or 3-day-old biofilms or as planktonic suspensions, to concentrations of chlorhexidine digluconate, cetylpyridinium chloride or triclosan used in commercial mouthwashes were compared. Three-day biofilms were the most resistant form of t...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-5-425
更新日期:1997-05-01 00:00:00
abstract::Acute hypercytokinaemia represents an imbalance of pro-inflammatory and anti-inflammatory cytokines, and is believed to be responsible for the development of acute respiratory distress syndrome and multiple organ failure in severe cases of avian (H5N1) influenza. Although neuraminidase inhibitors are effective in trea...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.47124-0
更新日期:2007-07-01 00:00:00
abstract::Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.04991-0
更新日期:2003-01-01 00:00:00
abstract::Different aspects of the terms strain, clone and species are discussed. The term strain is commonly used to denote a pure culture - here called 'the strain in the taxonomic sense' - but does also refer to a natural concept closely related to the clone. The term clone on the other hand is used both in a general and in ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/0022-1317-49-5-397
更新日期:2000-05-01 00:00:00
abstract::A large molecular-weight fraction of Pseudomonas aeruginosa culture filtrate protected rats and mice against a lethal infection with a heterologous serotype, and to some extent against Escherichia coli and Listeria monocytogenes. The active components were obtained from cultures grown for several days in a simple synt...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-13-2-201
更新日期:1980-05-01 00:00:00
abstract::Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic Escherichia coli O157 : H7 infection in humans. Shiga toxins are classical AB(5)-type exotoxins, consisting of a globotriaosylceramide (Gb(3))-binding B subunit p...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47282-0
更新日期:2007-11-01 00:00:00
abstract::Competition, competitive exclusion and displacement of eight strains of Escherichia coli and Salmonella spp. by Lactobacillus rhamnosus GG and Lactobacillus casei Shirota from adhesion on human intestinal mucus glycoproteins and Caco-2 cell surfaces were studied. Lactobacilli were able to compete with, exclude and dis...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05009-0
更新日期:2003-10-01 00:00:00
abstract::The ability of cefotaxime, ciprofloxacin, piperacillin and tobramycin to cause release of endotoxin was examined in vitro with cultures of Enterobacter cloacae and Escherichia coli. Endotoxin was measured by a quantitative limulus amoebocyte lysate assay and its presence was confirmed by silver staining of the lipopol...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-40-1-23
更新日期:1994-01-01 00:00:00
abstract::A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000256
更新日期:2016-06-01 00:00:00
abstract::Meticillin-resistant Staphylococcus aureus (MRSA) was unexpectedly isolated from a pig used for streptozotocin-induced diabetes research. To investigate the possible source of the MRSA isolate, nasal swabs were obtained from the animal herd, and from animal holding rooms, and veterinary and research staff involved in ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47283-0
更新日期:2007-08-01 00:00:00
abstract::In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000120
更新日期:2015-09-01 00:00:00
abstract::Limited digestion of staphylococcal enterotoxin B (SEB) with trypsin resulted in the generation of a 12-Kda amino-terminal fragment and a 17-Kda carboxy-terminal fragment which were isolated by preparative iso-electric focusing. The carboxy-terminal fragment exhibited significant mitogenic activity for murine splenocy...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-3-155
更新日期:1992-03-01 00:00:00