Abstract:
:G protein-coupled receptors (GPCRs) constitute the largest family of cell surface receptors. They can exist and act as dimers, but the requirement of dimers for agonist-induced signal initiation and structural dynamics remains largely unknown. Frizzled 6 (FZD6) is a member of Class F GPCRs, which bind WNT proteins to initiate signaling. Here, we show that FZD6 dimerizes and that the dimer interface of FZD6 is formed by the transmembrane α-helices four and five. Most importantly, we present the agonist-induced dissociation/re-association of a GPCR dimer through the use of live cell imaging techniques. Further analysis of a dimerization-impaired FZD6 mutant indicates that dimer dissociation is an integral part of FZD6 signaling to extracellular signal-regulated kinases1/2. The discovery of agonist-dependent dynamics of dimers as an intrinsic process of receptor activation extends our understanding of Class F and other dimerizing GPCRs, offering novel targets for dimer-interfering small molecules.Frizzled 6 (FZD6) is a G protein-coupled receptor (GPCR) involved in several cellular processes. Here, the authors use live cell imaging and spectroscopy to show that FZD6 forms dimers, whose association is regulated by WNT proteins and that dimer dissociation is crucial for FZD6 signaling.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Petersen J,Wright SC,Rodríguez D,Matricon P,Lahav N,Vromen A,Friedler A,Strömqvist J,Wennmalm S,Carlsson J,Schulte Gdoi
10.1038/s41467-017-00253-9subject
Has Abstractpub_date
2017-08-09 00:00:00pages
226issue
1issn
2041-1723pii
10.1038/s41467-017-00253-9journal_volume
8pub_type
杂志文章abstract::Carbon forms one of nature's strongest chemical bonds; its allotropes having provided some of the most exciting scientific discoveries in recent times. The possibility of inter-allotropic transformations/hybridization of carbon is hence a topic of immense fundamental and technological interest. Such modifications usua...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5941
更新日期:2014-09-15 00:00:00
abstract::Surface charge density is the key factor for developing high performance triboelectric nanogenerators (TENG). The previously invented charge excitation TENG provides a most efficient way to achieve maximum charge output of a TENG device. Herein, criteria to quantitatively evaluate the contact efficiency and air breakd...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15368-9
更新日期:2020-03-27 00:00:00
abstract::Cytidine base editors (CBEs) and adenine base editors (ABEs), composed of a cytidine deaminase or an evolved adenine deaminase fused to Cas9 nickase, enable the conversion of C·G to T·A or A·T to G·C base pair in organisms, respectively. Here, we show that BE3 and ABE7.10 systems can achieve a targeted mutation effici...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05232-2
更新日期:2018-07-13 00:00:00
abstract::Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorog...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10855-0
更新日期:2019-06-27 00:00:00
abstract::Thrombocytosis is present in more than 30% of patients with solid malignancies and correlates with worsened patient survival. Tumor cell interaction with various cellular components of the tumor microenvironment including platelets is crucial for tumor growth and metastasis. Although it is known that platelets can inf...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-00411-z
更新日期:2017-08-21 00:00:00
abstract::Plants can produce organs throughout their entire life from pluripotent stem cells located at their growing tip, the shoot apical meristem (SAM). At the time of flowering, the SAM of Arabidopsis thaliana switches fate and starts producing flowers instead of leaves. Correct timing of flowering in part determines reprod...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15120
更新日期:2017-05-17 00:00:00
abstract::Mast cells are critical effectors of allergic inflammation and protection against parasitic infections. We previously demonstrated that transcription factors GATA2 and MITF are the mast cell lineage-determining factors. However, it is unclear whether these lineage-determining factors regulate chromatin accessibility a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20766-0
更新日期:2021-01-21 00:00:00
abstract::The non-deterministic nature of photon sources is a key limitation for single-photon quantum processors. Spatial multiplexing overcomes this by enhancing the heralded single-photon yield without enhancing the output noise. Here the intrinsic statistical limit of an individual source is surpassed by spatially multiplex...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3582
更新日期:2013-01-01 00:00:00
abstract::Natriuretic peptides (NPs) are important hormones that regulate multiple cellular functions including cardiovascular physiology. In the heart, two natriuretic peptide receptors NPR1 and NPR2 act as membrane guanylyl cyclases to produce 3',5'-cyclic guanosine monophosphate (cGMP). Although both receptors protect from c...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04891-5
更新日期:2018-06-22 00:00:00
abstract::The temperature-jump technique, in which the sample is rapidly heated by a powerful laser pulse, has been widely used to probe the fast dynamics of folding of proteins and nucleic acids. However, the existing temperature-jump setups tend to involve sophisticated and expensive instrumentation, while providing only mode...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6737
更新日期:2014-12-17 00:00:00
abstract::Biological tissues exhibit complex spatial heterogeneity that directs the functions of multicellular organisms. Quantifying protein expression is essential for elucidating processes within complex biological assemblies. Imaging mass spectrometry (IMS) is a powerful emerging tool for mapping the spatial distribution of...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13858-z
更新日期:2020-01-07 00:00:00
abstract::Despite highly promising characteristics of three-dimensionally (3D) nanostructured catalysts for the oxygen evolution reaction (OER) in polymer electrolyte membrane water electrolyzers (PEMWEs), universal design rules for maximizing their performance have not been explored. Here we show that woodpile (WP)-structured ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18686-0
更新日期:2020-10-01 00:00:00
abstract::Polymers bearing dynamic covalent bonds may exhibit dynamic properties, such as self-healing, shape memory and environmental adaptation. However, most dynamic covalent chemistries developed so far require either catalyst or change of environmental conditions to facilitate bond reversion and dynamic property change in ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4218
更新日期:2014-01-01 00:00:00
abstract::RNA-binding proteins (RBPs) integrate the processing of RNAs into post-transcriptional gene regulation, but the direct contribution of them to myeloid cell specification is poorly understood. Here, we report the first global RBP transcriptomic analysis of myeloid differentiation by combining RNA-seq analysis with myel...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01425-3
更新日期:2017-11-10 00:00:00
abstract::A common analysis of single-cell sequencing data includes clustering of cells and identifying differentially expressed genes (DEGs). How cell clusters are defined has important consequences for downstream analyses and the interpretation of results, but is often not straightforward. To address this difficulty, we prese...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17900-3
更新日期:2020-08-28 00:00:00
abstract::The time-dependent response of structural materials dominates our aging infrastructure's life expectancy and has important resilience implications. For calcium-silicate-hydrates, the glue of cement, nanoscale mechanisms underlying time-dependent phenomena are complex and remain poorly understood. This complexity origi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04174-z
更新日期:2018-05-03 00:00:00
abstract::The accuracy of state-of-the-art atomic clocks is derived from the insensitivity of narrow optical atomic resonances to environmental perturbations. Two such resonances in singly ionized lutetium have been identified with potentially lower sensitivities compared to other clock candidates. Here we report measurement of...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04079-x
更新日期:2018-04-25 00:00:00
abstract::Superchiral light, generated by the interference of two counter-propagating circularly polarized light (CPL) with same frequency, opposite handedness and different intensity, exhibits enhanced dissymmetry in its interaction with chiral molecules, and has the potential for ultrasensitive detection and characterization ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07533-y
更新日期:2018-11-30 00:00:00
abstract::Is there some kind of historical memory and folk wisdom that ensures that a community remembers about very extreme phenomena, such as catastrophic floods, and learns to establish new settlements in safer locations? We tested a unique set of empirical data on 1293 settlements founded in the course of nine centuries, du...
journal_title:Nature communications
pub_type: 历史文章,杂志文章
doi:10.1038/s41467-019-09102-3
更新日期:2019-03-07 00:00:00
abstract::Exploitation of the oxidation behaviour in an environmentally sensitive semiconductor is significant to modulate its electronic properties and develop unique applications. Here, we demonstrate a native oxidation-inspired InSe field-effect transistor as an artificial synapse in device level that benefits from the boost...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16766-9
更新日期:2020-06-12 00:00:00
abstract::The spindle assembly checkpoint (SAC) arrests cells in mitosis by sensing unattached kinetochores, until all chromosomes are bi-oriented by spindle microtubules. Kinetochore accumulation of the SAC component Mad1-Mad2 is crucial for SAC activation. However, the mechanism by which Mad1-Mad2 accumulation at kinetochores...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10151
更新日期:2016-01-08 00:00:00
abstract::Vertical transmission accounts for most human immunodeficiency virus (HIV) infection in children, and treatments for newborns are needed to abrogate infection or limit disease progression. We showed previously that short-term broadly neutralizing antibody (bNAb) therapy given 24 h after oral exposure cleared simian-hu...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13972-y
更新日期:2020-01-07 00:00:00
abstract::A non-classical light source emitting pairs of identical photons represents a versatile resource of interdisciplinary importance with applications in quantum optics and quantum biology. To date, photon twins have mostly been generated using parametric downconversion sources, relying on Poissonian number distributions,...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14870
更新日期:2017-04-03 00:00:00
abstract::Respiration by soil bacteria and fungi is one of the largest fluxes of carbon (C) from the land surface. Although this flux is a direct product of microbial metabolism, controls over metabolism and their responses to global change are a major uncertainty in the global C cycle. Here, we explore an in silico approach to...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11488-z
更新日期:2019-08-08 00:00:00
abstract::Coalescence is an essential phenomenon that governs the equilibrium behaviour in a variety of systems from intercellular transport to planetary formation. In this report, we study coalescence pathways of circularly shaped two-dimensional colloidal membranes, which are one rod-length-thick liquid-like monolayers of ali...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4063
更新日期:2014-01-01 00:00:00
abstract::Closed timelike curves are striking predictions of general relativity allowing for time-travel. They are afflicted by notorious causality issues (e.g. grandfather's paradox). Quantum models where a qubit travels back in time solve these problems, at the cost of violating quantum theory's linearity-leading e.g. to univ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-08100-1
更新日期:2019-01-14 00:00:00
abstract::Modern beam shaping techniques have enabled the generation of optical fields displaying a wealth of structural features, which include three-dimensional topologies such as Möbius, ribbon strips and knots. However, unlike simpler types of structured light, the topological properties of these optical fields have hithert...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18792-z
更新日期:2020-10-09 00:00:00
abstract::Amidoxime-functionalized polymeric adsorbents are the current state-of-the-art materials for collecting uranium (U) from seawater. However, marine tests show that vanadium (V) is preferentially extracted over U and many other cations. Herein, we report a complementary and comprehensive investigation integrating ab ini...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01443-1
更新日期:2017-11-16 00:00:00
abstract::Deep subwavelength integration of high-definition plasmonic nanostructures is of key importance in the development of future optical nanocircuitry for high-speed communication, quantum computation and lab-on-a-chip applications. To date, the experimental realization of proposed extended plasmonic networks consisting o...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1143
更新日期:2010-01-01 00:00:00
abstract::Two waves of DNA methylation reprogramming occur during mammalian embryogenesis; during preimplantation development and during primordial germ cell (PGC) formation. However, it is currently unclear how evolutionarily conserved these processes are. Here we characterise the DNA methylomes of zebrafish PGCs at four devel...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10895-6
更新日期:2019-07-11 00:00:00