Fetal cells in the blood of pregnant women: detection and enrichment by fluorescence-activated cell sorting.

abstract：:Mouse tumor C1300 has been established in tissue culture. The cells have a round cell morphology in both the subcutaneous tumor and in suspension culture. However, when given a surface on which to attach, they send out processes up to 3 mm in length and assume the morphology of mature neurons. The attached cells are s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schubert D,Humphreys S,Baroni C,Cohn M

更新日期：1969-09-01 00:00:00

abstract：:Heavy atoms, such as bromine or iodine, perturb the excited-state properties of aromatic chromophores through a spin-orbital coupling mechanism. In the present work the use of specifically directed spin-orbital probes to study subtle structural relationships in biopolymers is described. Heavy atoms are introduced into...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galley WC,Purkey RM

更新日期：1972-08-01 00:00:00

abstract：:Extensive studies have detailed the molecular regulation of individual components of the hemostatic system, including platelets, coagulation factors, and regulatory proteins. Questions remain, however, about how these elements are integrated at the systems level within a rapidly changing physical environment. To answe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tomaiuolo M,Matzko CN,Poventud-Fuentes I,Weisel JW,Brass LF,Stalker TJ

更新日期：2019-02-05 00:00:00

abstract：:This article briefly summarizes the research activities in the field of hydrogen storage in sorbent materials and reports our recent works and future directions for the design of such materials. Distinct features of sorption-based hydrogen storage methods are described compared with metal hydrides and complex chemical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park N,Choi K,Hwang J,Kim DW,Kim DO,Ihm J

更新日期：2012-12-04 00:00:00

abstract：:For 2 < k [abstract: see text] we define lifts of cuspidal Poincaré series in S(k)(Gamma(0)(N)) to weight 2 - k harmonic weak Maass forms. This construction answers a question of Dyson by providing the general framework "explaining" Ramanujan's mock theta functions. As an application, we show that the number of partit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bringmann K,Ono K

更新日期：2007-03-06 00:00:00

abstract：:Cerebellar granule cells grown in the presence of a serum complex differentiate but are resistant to the lethal action of excitatory amino acids. When these cells are grown also in the presence of insulin-like growth factor I (IGF-I) they become fully susceptible to the toxic, lethal action of glutamate. The glutamate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calissano P,Ciotti MT,Battistini L,Zona C,Angelini A,Merlo D,Mercanti D

更新日期：1993-09-15 00:00:00

abstract：:A juvenile male zebra finch, Taeniopygia guttata, kept singly with its father develops a fairly complete imitation of the father's song. The imitation is less complete when other male siblings are present, possibly because as imitation commences, model abundance increases. Here we examine the consequences of allowing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tchernichovski O,Lints T,Mitra PP,Nottebohm F

更新日期：1999-10-26 00:00:00

abstract：:Ectopic activation of the Wnt signaling pathway is highly oncogenic for many human tissues. Here, we show that ectopic Wnt signaling increases the effective stem cell activity in mouse mammary glands in vivo. Furthermore, Wnt effectors induce the accumulation of mouse mammary epithelial progenitors (assayed by Hoechst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu BY,McDermott SP,Khwaja SS,Alexander CM

更新日期：2004-03-23 00:00:00

abstract：:Glutamate transporters clear synaptically released glutamate to maintain precise communication between neurons and limit glutamate neurotoxicity. Although much progress has been made on the topology, structure, and function of these carriers, few studies have addressed large-scale structural motions collectively assoc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang J,Shrivastava IH,Watts SD,Bahar I,Amara SG

更新日期：2011-09-13 00:00:00

abstract：:I have determined the nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase. This gene codes for a protein of 286 amino acid residues. The first 23 amino acids presumably form a signal for secretion, because they do not appear in the matur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sutcliffe JG

更新日期：1978-08-01 00:00:00

abstract：:A new nondefective hybrid virus has been plaque-isolated from the Ad.2-SV40 hybrid population. This virus replicates efficiently with one-hit kinetics in both human embryonic kidney and African green monkey kidney cells, induces an SV40 specific antigen which is detectable by immunofluorescence and complement-fixation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis AM Jr,Levin MJ,Wiese WH,Crumpacker CS,Henry PH

更新日期：1969-08-01 00:00:00

abstract：:Many animals employ sex pheromones to find mating partners during their reproductive seasons. However, most sex pheromones of vertebrates remain to be identified. Over the past 20 years, steroids and prostaglandins have been identified as sex pheromones in several fishes. These pheromones are broadly termed "hormonal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yambe H,Kitamura S,Kamio M,Yamada M,Matsunaga S,Fusetani N,Yamazaki F

更新日期：2006-10-17 00:00:00

abstract：:Feedback loops are ubiquitous features of biological networks and can produce significant phenotypic heterogeneity, including a bimodal distribution of gene expression across an isogenic cell population. In this work, a combination of experiments and computational modeling was used to explore the roles of multiple fee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Venturelli OS,El-Samad H,Murray RM

更新日期：2012-11-27 00:00:00

abstract：:MAC5 is a component of the conserved MOS4-associated complex. It plays critical roles in development and immunity. Here we report that MAC5 is required for microRNA (miRNA) biogenesis. MAC5 interacts with Serrate (SE), which is a core component of the microprocessor that processes primary miRNA transcripts (pri-miRNAs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li S,Li M,Liu K,Zhang H,Zhang S,Zhang C,Yu B

更新日期：2020-09-22 00:00:00

abstract：:Conditional mutations such as temperature-sensitive (ts) mutations are important for the analysis of protein function but are often difficult, or impossible, to obtain. Here we present a simple method for generating conditional mutations based on the use of a protein-destabilizing genetic element in combination with s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park EC,Finley D,Szostak JW

更新日期：1992-02-15 00:00:00

abstract：:L-type Ca(2+) channels play a critical role in regulating Ca(2+)-dependent signaling in cardiac myocytes, including excitation-contraction coupling; however, the subcellular localization of cardiac L-type Ca(2+) channels and their regulation are incompletely understood. Caveolae are specialized microdomains of the pla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balijepalli RC,Foell JD,Hall DD,Hell JW,Kamp TJ

更新日期：2006-05-09 00:00:00

abstract：:Shell shape and shell thickness of the intertidal snail Littorina obtusata changed markedly between 1871 and 1984 in northern New England. Shells collected prior to 1900 were high-spired with thin walls, whereas shells collected in 1982-84 were low-spired with thick walls. An intertidal crab (Carcinus maenas) which pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seeley RH

更新日期：1986-09-01 00:00:00

abstract：:Four overlapping cosmid clones were constructed that contain the complete genome of the attenuated Oka strain of VZV. Transfection of human melanoma cells with the four cosmids resulted in production of infectious VZV. A double-stranded oligonucleotide, encoding a stop codon in all three open reading frames, was inser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cohen JI,Seidel KE

更新日期：1993-08-01 00:00:00

abstract：:Hyperapobetalipoproteinemia is defined as the combination of a normal low density lipoprotein (LDL) cholesterol in the face of an increased LDL apolipoprotein B (apoB) protein. To examine the physical basis for the apparent disproportion between LDL cholesterol and apoB characteristic of this syndrome, we used density...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teng B,Thompson GR,Sniderman AD,Forte TM,Krauss RM,Kwiterovich PO Jr

更新日期：1983-11-01 00:00:00

abstract：:The beta-adrenergic receptor kinase (beta ARK) phosphorylates its membrane-associated receptor substrates, such as the beta-adrenergic receptor, triggering events leading to receptor desensitization. beta ARK activity is markedly stimulated by the isoprenylated beta gamma subunit complex of heterotrimeric guanine nucl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inglese J,Luttrell LM,Iñiguez-Lluhi JA,Touhara K,Koch WJ,Lefkowitz RJ

更新日期：1994-04-26 00:00:00

abstract：:Regulatory mechanisms for tissue repair and regeneration within damaged tissue have been extensively studied. However, the systemic regulation of tissue repair remains poorly understood. To elucidate tissue nonautonomous control of repair process, it is essential to induce local damage, independent of genetic manipula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kashio S,Obata F,Zhang L,Katsuyama T,Chihara T,Miura M

更新日期：2016-02-16 00:00:00

abstract：:Knowledge of how insects are actually affected by sex pheromones deployed throughout a crop so as to disrupt mating has lacked a mechanistic framework sufficient for guiding optimization of this environmentally friendly pest-control tactic. Major hypotheses are competitive attraction, desensitization, and camouflage. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller JR,McGhee PS,Siegert PY,Adams CG,Huang J,Grieshop MJ,Gut LJ

更新日期：2010-01-05 00:00:00

abstract：:Systemic delivery of bioactive molecules in the CNS is hampered by the blood-brain barrier, which has bottlenecked noninvasive physiological study of the brain and the development of CNS drugs. Here we report that irradiation with an ultrashort pulsed laser to the blood vessel wall induces transient leakage of blood p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi M,Ku T,Chong K,Yoon J,Choi C

更新日期：2011-05-31 00:00:00

abstract：:Cerebral arteriovenous malformations (AVMs) are common vascular malformations, which may result in hemorrhagic strokes and neurological deficits. Bone morphogenetic protein (BMP) and Notch signaling are both involved in the development of cerebral AVMs, but the cross-talk between the two signaling pathways is poorly u...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yao Y,Yao J,Radparvar M,Blazquez-Medela AM,Guihard PJ,Jumabay M,Boström KI

更新日期：2013-11-19 00:00:00

abstract：:Art forgeries have existed since antiquity, but with the recent rapidly expanding commercialization of art, the approach to art authentication has demanded increasingly sophisticated detection schemes. So far, the most conclusive criterion in the field of counterfeit detection is the scientific proof of material anach...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hendriks L,Hajdas I,Ferreira ESB,Scherrer NC,Zumbühl S,Smith GD,Welte C,Wacker L,Synal HA,Günther D

更新日期：2019-07-02 00:00:00

abstract：:Aqueous gel sieving chromatography on Sephadex G-10 of the Group IA cations (Li+, Na+, K+, Rb+, Cs+) plus NH4+ as the Cl- salts, in combination with previous results for the halide anions (F-, Cl-, Br-, I-) as the Na+ salts [Washabaugh, M.W. & Collins, K.D. (1986) J. Biol. Chem. 261, 12477-12485], leads to the followi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins KD

更新日期：1995-06-06 00:00:00

abstract：:Macropinocytosis is a highly conserved endocytic process by which extracellular fluid and solutes are internalized into cells. Macropinocytosis starts with the formation of membrane ruffles at the plasma membrane and ends with their closure. The transient and sequential emergence of phosphoinositides PI(3,4,5)P3 and P...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maekawa M,Terasaka S,Mochizuki Y,Kawai K,Ikeda Y,Araki N,Skolnik EY,Taguchi T,Arai H

更新日期：2014-03-18 00:00:00

abstract：:Kss1, a yeast mitogen-activated protein kinase (MAPK), in its unphosphorylated (unactivated) state binds directly to and represses Ste12, a transcription factor necessary for expression of genes whose promoters contain filamentous response elements (FREs) and genes whose promoters contain pheromone response elements (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bardwell L,Cook JG,Zhu-Shimoni JX,Voora D,Thorner J

更新日期：1998-12-22 00:00:00

abstract：:Profiling the dynamic interaction of p300 with proximal promoters of human T cells identified a class of genes that rapidly coassemble p300 and RNA polymerase II (pol II) following mitogen stimulation. Several of these p300 targets are immediate early genes, including FOS, implicating a prominent role for p300 in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Byun JS,Wong MM,Cui W,Idelman G,Li Q,De Siervi A,Bilke S,Haggerty CM,Player A,Wang YH,Thirman MJ,Kaberlein JJ,Petrovas C,Koup RA,Longo D,Ozato K,Gardner K

更新日期：2009-11-17 00:00:00

abstract：:Classical hypotheses regarding the evolutionary origin of paired appendages propose transformation of precursor structures (gill arches and lateral fin folds) into paired fins. During development, gnathostome paired appendages form as outgrowths of body wall somatopleure, a tissue composed of somatic lateral plate mes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tulenko FJ,McCauley DW,Mackenzie EL,Mazan S,Kuratani S,Sugahara F,Kusakabe R,Burke AC

更新日期：2013-07-16 00:00:00