Abstract:
:The phylum Actinobacteria constitutes one of the largest and anciently divergent phyla within the Bacteria domain. Actinobacterial diversity has been thoroughly researched in various environments due to its unique biotechnological potential. Such studies have focused mostly on soil communities, but more recently marine and extreme environments have also been explored, finding rare taxa and demonstrating dispersal limitation and biogeographic patterns for Streptomyces. To test the distribution of Actinobacteria populations on a small scale, we chose the extremely oligotrophic and biodiverse Cuatro Cienegas Basin (CCB), an endangered oasis in the Chihuahuan desert to assess the diversity and uniqueness of Actinobacteria in the Churince System with a culture-dependent approach over a period of three years, using nine selective media. The 16S rDNA of putative Actinobacteria were sequenced using both bacteria universal and phylum-specific primer pairs. Phylogenetic reconstructions were performed to analyze OTUs clustering and taxonomic identification of the isolates in an evolutionary context, using validated type species of Streptomyces from previously phylogenies as a reference. Rarefaction analysis for total Actinobacteria and for Streptomyces isolates were performed to estimate species' richness in the intermediate lagoon (IL) in the oligotrophic Churince system. A total of 350 morphologically and nutritionally diverse isolates were successfully cultured and characterized as members of the Phylum Actinobacteria. A total of 105 from the total isolates were successfully subcultured, processed for DNA extraction and 16S-rDNA sequenced. All strains belong to the order Actinomycetales, encompassing 11 genera of Actinobacteria; the genus Streptomyces was found to be the most abundant taxa in all the media tested throughout the 3-year sampling period. Phylogenetic analysis of our isolates and another 667 reference strains of the family Streptomycetaceae shows that our isolation effort produced 38 unique OTUs in six new monophyletic clades. This high biodiversity and uniqueness of Actinobacteria in an extreme oligotrophic environment, which has previously been reported for its diversity and endemicity, is a suggestive sign of microbial biogeography of Actinobacteria and it also represents an invaluable source of biological material for future ecological and bioprospecting studies.
journal_name
PeerJjournal_title
PeerJauthors
Arocha-Garza HF,Canales-Del Castillo R,Eguiarte LE,Souza V,De la Torre-Zavala Sdoi
10.7717/peerj.3247subject
Has Abstractpub_date
2017-05-02 00:00:00pages
e3247issn
2167-8359pii
3247journal_volume
5pub_type
杂志文章相关文献
PeerJ文献大全abstract:BACKGROUND:Amoebiasis is the third most common parasitic cause of morbidity and mortality, particularly in countries with poor hygienic settings. There exists an ambiguity in the diagnosis of amoebiasis, and hence there arises a necessity for a better diagnostic approach. Serine-rich Entamoeba histolyticaprotein (SREHP...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3160
更新日期:2017-06-28 00:00:00
abstract::Because cancer evolution underlies the therapeutic difficulties of cancer, it is clinically important to understand the evolutionary dynamics of cancer. Thus far, a number of evolutionary processes have been proposed to be working in cancer evolution. However, there exists no simulation model that can describe the dif...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8842
更新日期:2020-04-08 00:00:00
abstract::Small interference RNA has been widely used to suppress gene expression. Three different short hairpin RNAs (shRNAs) against dopamine D1 receptor (Drd1), driven by mouse U6 promoter in self-complementary AAV8 vector (scAAV8), were used to silence mouse striatal Drd1 expression. Transduction of mouse striatum with all ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3905
更新日期:2017-10-31 00:00:00
abstract:OBJECTIVE:The purpose of this study was to assess the effects of age, body condition score (BCS) and muscle condition score (MCS) on indirect radial and coccygeal Doppler systolic arterial blood pressure (SAP) measurements in dogs. METHODS:Sixty-two privately-owned dogs were enrolled between June and July 2016. The BC...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3101
更新日期:2017-03-22 00:00:00
abstract::[This corrects the article DOI: 10.7717/peerj.3954.]. ...
journal_title:PeerJ
pub_type: 杂志文章,已发布勘误
doi:10.7717/peerj.3954/correction-1
更新日期:2017-11-14 00:00:00
abstract:Background:During a preliminary study, effects of 0, 20, 40, and 60 mM NaCl salinity were assessed on germination rate in relation to electrolyte leakage (EL) in sweet pepper. Results explored significant rises in ethylene evolution from seeds having more EL. It was, therefore, hypothesized that excessive ethylene bios...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8475
更新日期:2020-03-27 00:00:00
abstract::A new species of the genus Mesacanthion Filipjev, 1927 was discovered during a survey of natural beaches of Jeju Island in South Korea. The new species Mesacanthion jejuensissp. nov. shares general morphology of the genus such as the outer labial and cephalic setae being situated at the middle of cephalic capsule, wel...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8023
更新日期:2019-11-18 00:00:00
abstract:Background:Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or Vmem. Vmem plays a key role in numerous inter- and intrac...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6341
更新日期:2019-02-08 00:00:00
abstract::Seasonality impacts species distributions through changes of the environmental conditions that affect the presence of individuals at a given place. Although the dynamics of cave microclimates are well known, only a few studies have evaluated the effects of such dynamics on non-strictly cave species. Here we assessed i...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3169
更新日期:2017-05-10 00:00:00
abstract::The presence of fatigue has been shown to modify running biomechanics. Overall in terms of gender, women are at lower risk than men for sustaining running-related injuries, although it depends on the factors taken into account. One possible reason for these differences in the injury rate and location might be the diss...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4489
更新日期:2018-03-20 00:00:00
abstract::In highly biodiverse systems, such as coral reefs, prey species are faced with predatory threats from numerous species. Recognition of predators can be innate, or learned, and can help increase the chance of survival. Research suggests that parental exposure to increased predatory threats can affect the development, b...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9340
更新日期:2020-06-18 00:00:00
abstract::Circular RNA (circRNA) is an endogenous noncoding RNA with a covalently closed cyclic structure. Based on their components, circRNAs are divided into exonic circRNAs, intronic circRNAs, and exon-intron circRNAs. CircRNAs have well-conserved sequences and often have high stability due to their resistance to exonuclease...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5503
更新日期:2018-08-24 00:00:00
abstract::Changes in behavior are often the proximate response of animals to human disturbance, with variability in tolerance levels leading some species to exhibit striking shifts in life history, fitness, and/or survival. Thus, elucidating the effects of disturbance on animal behavior, and how this varies among taxonomically ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6956
更新日期:2019-06-07 00:00:00
abstract::Background. Neonatal hyperbilirubinemia (HB) may cause severe neurological damage, but serious consequences are effectively controlled by phototherapy and blood exchange transfusion. HB is still a serious health problem in economically compromised parts of the world. The long term outcome has been regarded favorable b...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.294
更新日期:2014-03-04 00:00:00
abstract::Triple negative breast cancer (TNBC) is a belligerent carcinoma that is unresponsive to targeted receptor therapies. Development of new treatment strategies would benefit from an expanded repertoire of in vitro cell culture systems, such as those that support tridimensional growth in the presence of hydrogel scaffolds...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4340
更新日期:2018-02-16 00:00:00
abstract::Wild greens can contribute to the human diet as an important source of essential nutrients and drugs. Since environmental factors, including soil properties, may affect the chemical composition of plants, it is necessary among others to assess various habitats in terms of their usefulness for wild plant harvesting and...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.10233
更新日期:2020-11-24 00:00:00
abstract::Callinectes sapidus Rathbun, 1896 is a western Atlantic species with a disjointed natural geographic range from Massachusetts, USA to Venezuela (distribution area 1) and from Alagoas, Brazil to northern Argentina (distribution area 2). It is the only species of portunid crab commercially harvested in the continental U...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7827
更新日期:2019-11-05 00:00:00
abstract::In the Balearic Islands, different trammel net designs have been adopted to promote fisheries sustainability and reduce discards. Here, we compare the catch performance of three trammel net designs targeting the spiny lobster Palinurus elephas in terms of biomass, species composition and revenue from commercial catche...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4707
更新日期:2018-05-17 00:00:00
abstract::Microbial transglutaminase (mTG) was used as a crosslinking agent in the preparation of gelatin sponges. The physical properties of the materials were evaluated by measuring their material porosity, water absorption, and elastic modulus. The stability of the sponges were assessed via hydrolysis and enzymolysis. To stu...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3665
更新日期:2017-08-09 00:00:00
abstract:Background:Forest ecosystems are considered among the largest terrestrial carbon sinks. The dynamics of forest carbon depend on where the carbon is stored and its responses to environmental factors, as well as the physiology of the trees. Thus, threatened forest regions with high biodiversity have great scientific impo...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7085
更新日期:2019-06-11 00:00:00
abstract::Background. Low molecular weight heparin (LMWH) is an effective anti-coagulant for thrombotic events. However, due to its predominant renal clearance, there are concerns that it might be associated with increased bleeding in patients with renal disease. Objectives. We systematically evaluated the efficacy and safety o...
journal_title:PeerJ
pub_type: 杂志文章,评审
doi:10.7717/peerj.835
更新日期:2015-03-10 00:00:00
abstract:BACKGROUND:The scholarly publication landscape is changing rapidly. We investigated whether the introduction of an institutional publications officer might help facilitate better knowledge of publication topics and related resources, and effectively support researchers to publish. METHODS:In September 2015, a purpose-...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3294
更新日期:2017-05-31 00:00:00
abstract::Cold-water corals provide an important habitat for a rich fauna along the continental margins and slopes. Although these azooxanthellate corals are considered particularly sensitive to ocean acidification, their responses to natural variations in pH and aragonite saturation are largely unknown due to the difficulty of...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.194
更新日期:2013-10-29 00:00:00
abstract::Gymnosperms such as ginkgo, conifers, cycads, and gnetophytes are vital components of land ecosystems, and they have significant economic and ecologic value, as well as important roles as forest vegetation. In this study, we investigated the structural variation and evolution of chloroplast transfer RNAs (tRNAs) in gy...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.10312
更新日期:2020-11-25 00:00:00
abstract::We provided chimpanzees (Pan troglodytes) with the ability to improve the quality of food rewards they received in a dyadic test of inequity. We were interested to see if this provision influenced their responses and, if so, whether it was mediated by a social partner's outcomes. We tested eight dyads using an exchang...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.165
更新日期:2013-09-17 00:00:00
abstract::We describe a new iguanodontian ornithopod, Choyrodon barsboldi gen. et sp. nov. from the Albian-aged Khuren Dukh Formation of Mongolia based on several partial skeletons interpreted to represent a subadult growth stage based on osteohistological features. This new taxon is diagnosed by many autapomorphies of the maxi...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5300
更新日期:2018-08-03 00:00:00
abstract::We have previously observed that methane supplied to lake sediment microbial communities as a substrate not only causes a response by bona fide methanotrophic bacteria, but also by non-methane-oxidizing bacteria, especially by members of the family Methylophilaceae. This result suggested that methane oxidation in this...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.801
更新日期:2015-02-24 00:00:00
abstract::The Clarkia lagerstätte (Latah Formation) of Idaho is well known for its beautifully preserved plant fossils as well as a fauna of insects and fish. Here we present the first known tetrapod fossil from these deposits. This specimen, recovered from the lower anoxic zone of the beds, is preserved as a carbonaceous film ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4880
更新日期:2018-06-08 00:00:00
abstract::Background. The ReproQuestionnaire (ReproQ) measures the client's experience with maternity care, following the WHO responsiveness model. In 2015, the ReproQ was appointed as national client experience questionnaire and will be added to the national list of indicators in maternity care. For using the ReproQ in quality...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2092
更新日期:2016-07-13 00:00:00
abstract::Ocean acidification threatens the foundation of tropical coral reefs. This study investigated three aspects of ocean acidification: (i) the rates at which perforate and imperforate coral-colony skeletons passively dissolve when pH is 7.8, which is predicted to occur globally by 2100, (ii) the rates of passive dissolut...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.208
更新日期:2013-11-21 00:00:00