Membrane potential (Vmem) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation.

Abstract:

Background:Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or Vmem. Vmem plays a key role in numerous inter- and intracellular functions that regulate cell behaviors like proliferation, differentiation and migration, all playing a critical role in embryonic development, healing, and regeneration. Methods:With the goal of analyzing the changes in Vmem during cell proliferation and differentiation, here we used direct current electrical stimulation (EStim) to promote cell proliferation and differentiation and simultaneously tracked the corresponding changes in Vmem in adipose derived mesenchymal stem cells (AT-MSC). Results:We found that EStim caused increased AT-MSC proliferation that corresponded to Vmem depolarization and increased osteogenic differentiation that corresponded to Vmem hyperpolarization. Taken together, this shows that Vmem changes associated with EStim induced cell proliferation and differentiation can be accurately tracked during these important cell functions. Using this tool to monitor Vmem changes associated with these important cell behaviors we hope to learn more about how these electrochemical cues regulate cell function with the ultimate goal of developing new EStim based treatments capable of controlling healing and regeneration.

journal_name

PeerJ

journal_title

PeerJ

authors

Bhavsar MB,Cato G,Hauschild A,Leppik L,Costa Oliveira KM,Eischen-Loges MJ,Barker JH

doi

10.7717/peerj.6341

subject

Has Abstract

pub_date

2019-02-08 00:00:00

pages

e6341

issn

2167-8359

pii

6341

journal_volume

7

pub_type

杂志文章

相关文献

PeerJ文献大全
  • In vitro performance in cotton plants with different genetic backgrounds: the case of Gossypium hirsutum in Mexico, and its implications for germplasm conservation.

    abstract::One of the best ex situ conservation strategies for wild germplasm is in vitro conservation of genetic banks. The success of in vitro conservation relies heavily on the micropropagation or performance of the species of interest. In the context of global change, crop production challenges and climate change, we face a ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7017

    authors: Hernández-Terán A,Wegier A,Benítez M,Lira R,Sosa Fuentes TG,Escalante AE

    更新日期:2019-06-10 00:00:00

  • Monitoring body condition score of reintroduced banteng (Bos javanicus D'Alton, 1923) into Salakphra Wildlife Sanctuary, Thailand.

    abstract:Background:Banteng (Bos javanicus d'Alton 1823) are an endangered species, highly sensitive to habitat structure and quality. In many areas, banteng were extinct and needed to be reintroduced to restore their population. Thus, understanding the responses of body condition of reintroduced banteng to their habitat was im...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9041

    authors: Kongsurakan P,Chaiyarat R,Nakbun S,Thongthip N,Anuracpreeda P

    更新日期:2020-04-23 00:00:00

  • NeisseriaBase: a specialised Neisseria genomic resource and analysis platform.

    abstract::Background. The gram-negative Neisseria is associated with two of the most potent human epidemic diseases: meningococcal meningitis and gonorrhoea. In both cases, disease is caused by bacteria colonizing human mucosal membrane surfaces. Overall, the genus shows great diversity and genetic variation mainly due to its a...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1698

    authors: Zheng W,Mutha NV,Heydari H,Dutta A,Siow CC,Jakubovics NS,Wee WY,Tan SY,Ang MY,Wong GJ,Choo SW

    更新日期:2016-03-17 00:00:00

  • A taxonomic review of the Late Jurassic eucryptodiran turtles from the Jura Mountains (Switzerland and France).

    abstract::Background. Eucryptodiran turtles from the Late Jurassic (mainly Kimmeridgian) deposits of the Jura Mountains (Switzerland and France) are among the earliest named species traditionally referred to the Plesiochelyidae, Thalassemydidae, and Eurysternidae. As such, they are a reference for the study of Late Jurassic euc...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.369

    authors: Anquetin J,Püntener C,Billon-Bruyat JP

    更新日期:2014-05-13 00:00:00

  • First insight into the viral community of the cnidarian model metaorganism Aiptasia using RNA-Seq data.

    abstract::Current research posits that all multicellular organisms live in symbioses with associated microorganisms and form so-called metaorganisms or holobionts. Cnidarian metaorganisms are of specific interest given that stony corals provide the foundation of the globally threatened coral reef ecosystems. To gain first insig...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4449

    authors: Brüwer JD,Voolstra CR

    更新日期:2018-03-01 00:00:00

  • Identification of key genes in non-small cell lung cancer by bioinformatics analysis.

    abstract:Background:Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors in the world, and it has become the leading cause of death of malignant tumors. However, its mechanisms are not fully clear. The aim of this study is to investigate the key genes and explore their potential mechanisms involving in ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8215

    authors: Zhang L,Peng R,Sun Y,Wang J,Chong X,Zhang Z

    更新日期:2019-12-12 00:00:00

  • Integrative analysis reveals pathways associated with sex reversal in Cynoglossus semilaevis.

    abstract::Sex reversal is a complex biological phenomenon exhibited by Cynoglossus semilaevis. Some genetic females may irreversibly convert to pseudomales, thus increasing aquaculture costs because males grow much more slowly than females. In this study, an integrative analysis of transcriptome and proteome was performed to co...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8801

    authors: Ye Z,Wang W,Zhang Y,Wang L,Cui Y,Li H

    更新日期:2020-03-19 00:00:00

  • Historical reconstruction of climatic and elevation preferences and the evolution of cloud forest-adapted tree ferns in Mesoamerica.

    abstract:BACKGROUND:Cloud forests, characterized by a persistent, frequent or seasonal low-level cloud cover and fragmented distribution, are one of the most threatened habitats, especially in the Neotropics. Tree ferns are among the most conspicuous elements in these forests, and ferns are restricted to regions in which minimu...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2696

    authors: Sosa V,Ornelas JF,Ramírez-Barahona S,Gándara E

    更新日期:2016-11-16 00:00:00

  • Fishing for vaccines against Vibrio cholerae using in silico pan-proteomic reverse vaccinology approach.

    abstract:Background:Cholera, an acute enteric infection, is a serious health challenge in both the underdeveloped and the developing world. It is caused by Vibrio cholerae after ingestion of fecal contaminated food or water. Cholera outbreaks have recently been observed in regions facing natural calamities (i.e., earthquake in ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6223

    authors: Rashid MI,Rehman S,Ali A,Andleeb S

    更新日期:2019-06-19 00:00:00

  • Application of dynamic expansion tree for finding large network motifs in biological networks.

    abstract::Network motifs play an important role in the structural analysis of biological networks. Identification of such network motifs leads to many important applications such as understanding the modularity and the large-scale structure of biological networks, classification of networks into super-families, and protein func...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6917

    authors: Patra S,Mohapatra A

    更新日期:2019-05-17 00:00:00

  • Is the future already here? The impact of climate change on the distribution of the eastern coral snake (Micrurus fulvius).

    abstract::Anthropogenic climate change is a significant global driver of species distribution change. Although many species have undergone range expansion at their poleward limits, data on several taxonomic groups are still lacking. A common method for studying range shifts is using species distribution models to evaluate curre...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4647

    authors: Archis JN,Akcali C,Stuart BL,Kikuchi D,Chunco AJ

    更新日期:2018-05-01 00:00:00

  • Halocarbon emissions by selected tropical seaweeds: species-specific and compound-specific responses under changing pH.

    abstract::Five tropical seaweeds, Kappaphycus alvarezii (Doty) Doty ex P.C. Silva, Padina australis Hauck, Sargassum binderi Sonder ex J. Agardh (syn. S. aquifolium (Turner) C. Agardh), Sargassum siliquosum J. Agardh and Turbinaria conoides (J. Agardh) Kützing, were incubated in seawater of pH 8.0, 7.8 (ambient), 7.6, 7.4 and 7...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2918

    authors: Mithoo-Singh PK,Keng FS,Phang SM,Leedham Elvidge EC,Sturges WT,Malin G,Abd Rahman N

    更新日期:2017-01-25 00:00:00

  • Future sea-level rise drives rocky intertidal habitat loss and benthic community change.

    abstract::The impacts of sea-level rise (SLR) are likely to be the greatest for ecosystems that exist at the land-sea interface, where small changes in sea-level could result in drastic changes in habitat availability. Rocky intertidal ecosystems possess a number of characteristics which make them highly vulnerable to changes i...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9186

    authors: Kaplanis NJ,Edwards CB,Eynaud Y,Smith JE

    更新日期:2020-05-29 00:00:00

  • A second HD mating type sublocus of Flammulina velutipes is at least di-allelic and active: new primers for identification of HD-a and HD-b subloci.

    abstract:Background:Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6292

    authors: Wang W,Mukhtar I,Chou T,Jiang S,Liu X,van Peer AF,Xie B

    更新日期:2019-02-22 00:00:00

  • Combined effect of 17β-estradiol and resveratrol against apoptosis induced by interleukin-1β in rat nucleus pulposus cells via PI3K/Akt/caspase-3 pathway.

    abstract:BACKGROUND:In previous studies, both 17β-estradiol (E2) and resveratrol (RES) were reported to protect intervertebral disc cells against aberrant apoptosis. Given that E2 has a better anti-apoptotic effect with more cancer risk and RES has an anti-apoptotic effect with less cancer risk, the combined use of E2 with RES ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1640

    authors: Yang SD,Ma L,Yang DL,Ding WY

    更新日期:2016-01-26 00:00:00

  • A new genus Vittaliana belonging to the tribe Opsiini (Hemiptera: Cicadellidae) from India and its molecular phylogeny.

    abstract::The new leafhopper genus Vittalianareticulata gen. nov., sp. nov., is described from India, and placed in the tribe Opsiini based on ocelli close to eyes, without carina on anterior margin of the face and bifurcate aedeagus with two gonopores. Phylogenetic analysis with maximum likelihood (ML) using IQtree v1.4.1 of c...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9515

    authors: Sunil,Meshram NM,Hashmi TR,Shashank PR

    更新日期:2020-08-27 00:00:00

  • Serum lipid level is not associated with symptomatic intracerebral hemorrhage after intravenous thrombolysis for acute ischemic stroke.

    abstract:Background:This study assessed whether serum lipid levels are associated with the risk of symptomatic intracerebral hemorrhage (sICH) and functional outcomes in patients with acute ischemic stroke after receiving intravenous thrombolysis. Methods:We retrospectively analyzed consecutive ischemic stroke patients who wer...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6021

    authors: Lin TC,Lin YK,Chen CI,Chan L,Chi NF,Yuan RY,Sheu JJ,Wei CR,Tsai JP,Yeh TH

    更新日期:2018-11-23 00:00:00

  • Does the sex difference in competitiveness decrease in selective sub-populations? A test with intercollegiate distance runners.

    abstract::Sex differences in some preferences and motivations are well established, but it is unclear whether they persist in selective sub-populations, such as expert financial decision makers, top scientists, or elite athletes. We addressed this issue by studying competitiveness in 1,147 varsity intercollegiate distance runne...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.884

    authors: Deaner RO,Lowen A,Rogers W,Saksa E

    更新日期:2015-04-21 00:00:00

  • Blood markers of endothelial dysfunction and their correlation to cerebrovascular reactivity in patients with chronic hepatitis C infection.

    abstract::Although liver cirrhosis and hepatocellular carcinoma are major consequences of hepatitis C (HCV), there has been an increasing number of studies examining extrahepatic manifestations, especially those caused by systemic chronic inflammation and metabolic complications that might predispose HCV patients to atheroscler...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10723

    authors: Pavicic Ivelja M,Dolic K,Tandara L,Perkovic N,Mestrovic A,Ivic I

    更新日期:2021-01-14 00:00:00

  • The use of bat houses as day roosts in macadamia orchards, South Africa.

    abstract::The loss of roost sites is one of the major drivers of the worldwide decline in bat populations and roost site preferences, either natural or artificially provided, are not well known for African bat species specifically. In this study we focus on the preference for different artificial roost sites by insectivorous ba...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6954

    authors: Weier SM,Linden VMG,Grass I,Tscharntke T,Taylor PJ

    更新日期:2019-05-22 00:00:00

  • The incidence rates of endometrial hyperplasia and endometrial cancer: a four-year population-based study.

    abstract:INTRODUCTION:The aim of this study was to determine the incidence rates of endometrial hyperplasia (EH) and endometrial cancer (EC) in the Republic of Korea using national insurance claim data generated from 2009 to 2012. MATERIALS AND METHODS:Data that were generated from 2009 to 2012 were sourced from the Korean Hea...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2374

    authors: Yuk JS

    更新日期:2016-08-24 00:00:00

  • Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs.

    abstract::Meniscal tears are a common cause of stifle lameness in dogs. Use of autologous synoviocytes from the affected stifle is an attractive cell source for tissue engineering replacement fibrocartilage. However, the diseased state of these cells may impede in vitro fibrocartilage formation. Synoviocytes from 12 osteoarthri...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.581

    authors: Warnock JJ,Bobe G,Duesterdieck-Zellmer KF

    更新日期:2014-09-30 00:00:00

  • Aberrant expression of two miRNAs promotes proliferation, hepatitis B virus amplification, migration and invasion of hepatocellular carcinoma cells: evidence from bioinformatic analysis and experimental validation.

    abstract:Background:As key negative regulators of gene expression, microRNAs (miRNAs) play an important role in the onset and progression of hepatocellular carcinoma (HCC). This study aimed to identify the miRNAs involved in HCC carcinogenesis and their regulated genes. Methods:The Gene Expression Omnibus (GEO) dataset (GSE108...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9100

    authors: Liu Y,Cao Y,Cai W,Wu L,Zhao P,Liu XG

    更新日期:2020-04-29 00:00:00

  • HAHap: a read-based haplotyping method using hierarchical assembly.

    abstract:Background:The need for read-based phasing arises with advances in sequencing technologies. The minimum error correction (MEC) approach is the primary trend to resolve haplotypes by reducing conflicts in a single nucleotide polymorphism-fragment matrix. However, it is frequently observed that the solution with the opti...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5852

    authors: Lin YY,Wu PC,Chen PL,Oyang YJ,Chen CY

    更新日期:2018-10-30 00:00:00

  • Allergic rhinitis, rather than asthma, might be associated with dental caries, periodontitis, and other oral diseases in adults.

    abstract:Background:The association between asthma (AS), allergic rhinitis (AR) and oral diseases remains inconclusive in adults. AS and AR often coexist. However, studies that investigate AS, AR together and their association with oral diseases are scarce. Methods:Data from 22,898 men and 28,541 women, aged 21 to 25 years, we...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7643

    authors: Ho SW,Lue KH,Ku MS

    更新日期:2019-09-10 00:00:00

  • VSEARCH: a versatile open source tool for metagenomics.

    abstract:BACKGROUND:VSEARCH is an open source and free of charge multithreaded 64-bit tool for processing and preparing metagenomics, genomics and population genomics nucleotide sequence data. It is designed as an alternative to the widely used USEARCH tool (Edgar, 2010) for which the source code is not publicly available, algo...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2584

    authors: Rognes T,Flouri T,Nichols B,Quince C,Mahé F

    更新日期:2016-10-18 00:00:00

  • Extraordinary centromeres: differences in the meiotic chromosomes of two rock lizards species Darevskia portschinskii and Darevskia raddei.

    abstract::According to the synthesis of 30 years of multidisciplinary studies, parthenogenetic species of rock lizards of genus Darevskia were formed as a result of different combination patterns of interspecific hybridization of the four bisexual parental species: Darevskia raddei, D. mixta, D. valentini, and D. portschinskii....

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6360

    authors: Spangenberg V,Arakelyan M,Galoyan E,Pankin M,Petrosyan R,Stepanyan I,Grishaeva T,Danielyan F,Kolomiets O

    更新日期:2019-01-30 00:00:00

  • Quantitative heterodonty in Crocodylia: assessing size and shape across modern and extinct taxa.

    abstract::Heterodonty in Crocodylia and closely related taxa has not been defined quantitatively, as the teeth rarely have been measured. This has resulted in a range of qualitative descriptors, with little consensus on the condition of dental morphology in the clade. The purpose of this study is to present a method for the qua...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6485

    authors: D'Amore DC,Harmon M,Drumheller SK,Testin JJ

    更新日期:2019-02-28 00:00:00

  • Repetitive low intensity magnetic field stimulation in a neuronal cell line: a metabolomics study.

    abstract::Low intensity repetitive magnetic stimulation of neural tissue modulates neuronal excitability and has promising therapeutic potential in the treatment of neurological disorders. However, the underpinning cellular and biochemical mechanisms remain poorly understood. This study investigates the behavioural effects of l...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4501

    authors: Hong I,Garrett A,Maker G,Mullaney I,Rodger J,Etherington SJ

    更新日期:2018-03-12 00:00:00

  • Next-generation sequencing of mixed genomic DNA allows efficient assembly of rearranged mitochondrial genomes in Amolops chunganensis and Quasipaa boulengeri.

    abstract::Recent improvements in next-generation sequencing (NGS) technologies can facilitate the obtainment of mitochondrial genomes. However, it is not clear whether NGS could be effectively used to reconstruct the mitogenome with high gene rearrangement. These high rearrangements would cause amplification failure, and/or ass...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2786

    authors: Yuan S,Xia Y,Zheng Y,Zeng X

    更新日期:2016-12-15 00:00:00