VSEARCH: a versatile open source tool for metagenomics.

Abstract:

BACKGROUND:VSEARCH is an open source and free of charge multithreaded 64-bit tool for processing and preparing metagenomics, genomics and population genomics nucleotide sequence data. It is designed as an alternative to the widely used USEARCH tool (Edgar, 2010) for which the source code is not publicly available, algorithm details are only rudimentarily described, and only a memory-confined 32-bit version is freely available for academic use. METHODS:When searching nucleotide sequences, VSEARCH uses a fast heuristic based on words shared by the query and target sequences in order to quickly identify similar sequences, a similar strategy is probably used in USEARCH. VSEARCH then performs optimal global sequence alignment of the query against potential target sequences, using full dynamic programming instead of the seed-and-extend heuristic used by USEARCH. Pairwise alignments are computed in parallel using vectorisation and multiple threads. RESULTS:VSEARCH includes most commands for analysing nucleotide sequences available in USEARCH version 7 and several of those available in USEARCH version 8, including searching (exact or based on global alignment), clustering by similarity (using length pre-sorting, abundance pre-sorting or a user-defined order), chimera detection (reference-based or de novo), dereplication (full length or prefix), pairwise alignment, reverse complementation, sorting, and subsampling. VSEARCH also includes commands for FASTQ file processing, i.e., format detection, filtering, read quality statistics, and merging of paired reads. Furthermore, VSEARCH extends functionality with several new commands and improvements, including shuffling, rereplication, masking of low-complexity sequences with the well-known DUST algorithm, a choice among different similarity definitions, and FASTQ file format conversion. VSEARCH is here shown to be more accurate than USEARCH when performing searching, clustering, chimera detection and subsampling, while on a par with USEARCH for paired-ends read merging. VSEARCH is slower than USEARCH when performing clustering and chimera detection, but significantly faster when performing paired-end reads merging and dereplication. VSEARCH is available at https://github.com/torognes/vsearch under either the BSD 2-clause license or the GNU General Public License version 3.0. DISCUSSION:VSEARCH has been shown to be a fast, accurate and full-fledged alternative to USEARCH. A free and open-source versatile tool for sequence analysis is now available to the metagenomics community.

journal_name

PeerJ

journal_title

PeerJ

authors

Rognes T,Flouri T,Nichols B,Quince C,Mahé F

doi

10.7717/peerj.2584

subject

Has Abstract

pub_date

2016-10-18 00:00:00

pages

e2584

issn

2167-8359

pii

2584

journal_volume

4

pub_type

杂志文章

相关文献

PeerJ文献大全
  • High cytoplasmic YAP1 expression predicts a poor prognosis in patients with colorectal cancer.

    abstract:Purpose:Yes associated protein 1 (YAP1), which is a standout amongst the most essential effectors of the Hippo pathway, assumes a vital part in a few kinds of cancer. However, whether YAP1 is an oncogene in CRC (colorectal cancer) remains controversial, and the association between the subcellular localization of YAP1 a...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10397

    authors: Dong T,Yuan Y,Xiang X,Sang S,Shen H,Wang L,Yang C,Li F,Li H,Zheng S

    更新日期:2020-11-19 00:00:00

  • Behavioural variability among captive African elephants in the use of the trunk while feeding.

    abstract::The Proboscideans, an order of mammals including elephants, are the largest of the Earth lands animals. One probable consequence of the rapid increase of their body size is the development of the trunk, a multitask highly sensitive organ used in a large repertoire of behaviours. The absence of bones in the trunk allow...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9678

    authors: Lefeuvre M,Gouat P,Mulot B,Cornette R,Pouydebat E

    更新日期:2020-08-18 00:00:00

  • Regional drivers of clutch loss reveal important trade-offs for beach-nesting birds.

    abstract::Coastal birds are critical ecosystem constituents on sandy shores, yet are threatened by depressed reproductive success resulting from direct and indirect anthropogenic and natural pressures. Few studies examine clutch fate across the wide range of environments experienced by birds; instead, most focus at the small si...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2460

    authors: Maslo B,Schlacher TA,Weston MA,Huijbers CM,Anderson C,Gilby BL,Olds AD,Connolly RM,Schoeman DS

    更新日期:2016-09-13 00:00:00

  • Clonal diversity and genetic variation of the sedge Carex nigra in an alpine fen depend on soil nutrients.

    abstract::In this study we analysed the impact of water regime and soil nutrients on the clonal diversity and genetic variation of the sedge Carex nigra in a central alpine fen. For our analysis, we established 16 study plots randomly distributed over the fen. We determined the exact elevation of each plot as an indicator for t...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8887

    authors: Reisch C,Meier S,Schmid C,Bartelheimer M

    更新日期:2020-06-03 00:00:00

  • Antimicrobial activity of Tachyplesin 1 against Burkholderia pseudomallei: an in vitro and in silico approach.

    abstract::Burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many conventional antibiotics. Therefore, alternative antimicrobial agents such as antimicrobial peptides (AMPs) are extensively studied to combat this issue. Our study aims to identify and understand the mode of action of the...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2468

    authors: Lee LF,Mariappan V,Vellasamy KM,Lee VS,Vadivelu J

    更新日期:2016-10-25 00:00:00

  • Identification of priority shorebird conservation areas in the Caribbean.

    abstract::Despite being geographically central to the Atlantic Americas Flyway for migratory birds, the Caribbean is often overlooked or underappreciated when addressing the conservation of North American shorebirds. To our knowledge, this is the first Caribbean-wide assessment of shorebird use in the region. We analyzed 211,01...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9831

    authors: Cañizares JR,Reed JM

    更新日期:2020-09-08 00:00:00

  • Two new species of Pseudopimelodus Bleeker, 1858 (Siluriformes: Pseudopimelodidae) from the Magdalena Basin, Colombia.

    abstract::The family Pseudopimelodidae is widely distributed in South America and includes 51 described species organized in seven genera. Only two of four species of the genus Pseudopimelodus have been recorded for the trans-Andean basins of Colombia, Pseudopimelodus bufonius and P. schultzi, whose similarity in external morph...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9723

    authors: Restrepo-Gómez AM,Rangel-Medrano JD,Márquez EJ,Ortega-Lara A

    更新日期:2020-09-03 00:00:00

  • Morphological analysis of dendrites and spines by hybridization of ridge detection with twin support vector machine.

    abstract::Dendritic spines are described as neuronal protrusions. The morphology of dendritic spines and dendrites has a strong relationship to its function, as well as playing an important role in understanding brain function. Quantitative analysis of dendrites and dendritic spines is essential to an understanding of the forma...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2207

    authors: Wang S,Chen M,Li Y,Shao Y,Zhang Y,Du S,Wu J

    更新日期:2016-07-20 00:00:00

  • Diverse responses of Symbiodinium types to menthol and DCMU treatment.

    abstract::To understand the mechanism of photosynthetic inhibition and generation of reactive oxygen species (ROS) in Symbiodinium types under stress, chemicals such as dichlorophenyl dimethylurea (DCMU) are widely used. Moreover, DCMU and recently menthol were used to generate aposymbiotic cnidarian hosts. While the effects of...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3843

    authors: Wang JT,Keshavmurthy S,Chu TY,Chen CA

    更新日期:2017-10-02 00:00:00

  • Protein signatures using electrostatic molecular surfaces in harmonic space.

    abstract::We developed a novel method based on the Fourier analysis of protein molecular surfaces to speed up the analysis of the vast structural data generated in the post-genomic era. This method computes the power spectrum of surfaces of the molecular electrostatic potential, whose three-dimensional coordinates have been eit...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.185

    authors: Carvalho CS,Vlachakis D,Tsiliki G,Megalooikonomou V,Kossida S

    更新日期:2013-10-22 00:00:00

  • Patterns of triclosan resistance in Vibrionaceae.

    abstract::The antimicrobial additive triclosan has been used in personal care products widely across the globe for decades. Triclosan resistance has been noted among Vibrio spp., but reports have been anecdotal and the extent of phenotypic triclosan resistance across the Vibrionaceae family has not been established. Here, tricl...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5170

    authors: Lydon KA,Robertson MJ,Lipp EK

    更新日期:2018-07-12 00:00:00

  • Comments and corrections on 3D modeling studies of locomotor muscle moment arms in archosaurs.

    abstract::In a number of recent studies we used computer modeling to investigate the evolution of muscle leverage (moment arms) and function in extant and extinct archosaur lineages (crocodilians, dinosaurs including birds and pterosaurs). These studies sought to quantify the level of disparity and convergence in muscle moment ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1272

    authors: Bates K,Maidment SC,Schachner ER,Barrett PM

    更新日期:2015-10-08 00:00:00

  • Changes in understory species occurrence of a secondary broadleaved forest after mass mortality of oak trees under deer foraging pressure.

    abstract::The epidemic of mass mortality of oak trees by Japanese oak wilt has affected secondary deciduous broadleaved forests that have been used as coppices in Japan. The dieback of oak trees formed gaps in the crown that would be expected to enhance the regeneration of shade-intolerant pioneer species. However, foraging by ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2816

    authors: Itô H

    更新日期:2016-12-22 00:00:00

  • The European and Japanese outbreaks of H5N8 derive from a single source population providing evidence for the dispersal along the long distance bird migratory flyways.

    abstract::The origin of recent parallel outbreaks of the high pathogenicity H5N8 avian flu virus in Europe and in Japan can be traced to a single source population, which has most likely been spread by migratory birds. By using Bayesian coalescent methods to analyze the DNA sequences of the virus to find the times for divergenc...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.934

    authors: Dalby AR,Iqbal M

    更新日期:2015-04-30 00:00:00

  • Parameter estimation in tree graph metabolic networks.

    abstract::We study the glycosylation processes that convert initially toxic substrates to nutritionally valuable metabolites in the flavonoid biosynthesis pathway of tomato (Solanum lycopersicum) seedlings. To estimate the reaction rates we use ordinary differential equations (ODEs) to model the enzyme kinetics. A popular choic...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2417

    authors: Astola L,Stigter H,Gomez Roldan MV,van Eeuwijk F,Hall RD,Groenenboom M,Molenaar JJ

    更新日期:2016-09-20 00:00:00

  • Pre- and post-experimental manipulation assessments confirm the increase in number of birds due to the addition of nest boxes.

    abstract::Secondary cavity nesting (SCN) birds breed in holes that they do not excavate themselves. This is possible where there are large trees whose size and age permit the digging of holes by primary excavators and only rarely happens in forest plantations, where we expected a deficit of both breeding holes and SCN species. ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1806

    authors: Cuatianquiz Lima C,Macías Garcia C

    更新日期:2016-03-15 00:00:00

  • Influence of hydropower stations on the water microbiota in the downstream of Jinsha River, China.

    abstract::Construction of hydropower stations has been an important approach to meet China's increasing power demand, but the impact of construction of hydropower stations on river microbiota is not fully understood. To evaluate this, the microbial composition from 18 sampling sites in the downstream of Jinsha River of China, u...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9500

    authors: Chen X,He D,Zhou L,Cao Y,Li Z

    更新日期:2020-07-16 00:00:00

  • Balancing plant conservation and agricultural production in the Ecuadorian Dry Inter-Andean Valleys.

    abstract:Background:Conserving both biodiversity and ecosystem services is a major goal of the Convention on Biological Diversity. Hotspots for biodiversity in the Andes significantly overlap with areas with dense human populations that sustain their economy through agricultural production. Therefore, developing management form...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6207

    authors: Quintana C,Girardello M,Balslev H

    更新日期:2019-02-13 00:00:00

  • Application of a high-quality, high-volume trap-neuter-return model of community cats in Seoul, Korea.

    abstract:Background:This study was performed to determine the characteristics of community cats that were admitted to trap-neuter-return(TNR) programs and the feasibility of the high-quality, high-volume spay/neuter (HQHVSN) model in Seoul, Korea. Methods:TNR programs were performed eight times from 2017 to 2018, and a total o...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8711

    authors: Cho Y,Kim K,Kim MS,Lee I

    更新日期:2020-03-05 00:00:00

  • Cellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit bunches.

    abstract:BACKGROUND:Aspergillus niger, along with many other lignocellulolytic fungi, has been widely used as a commercial workhorse for cellulase production. A fungal cellulase system generally includes three major classes of enzymes i.e., β-glucosidases, endoglucanases and cellobiohydrolases. Cellobiohydrolases (CBH) are vita...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3909

    authors: Woon JS,Mackeen MM,Illias RM,Mahadi NM,Broughton WJ,Murad AMA,Abu Bakar FD

    更新日期:2017-10-12 00:00:00

  • A six-microRNA signature can better predict overall survival of patients with esophagus adenocarcinoma.

    abstract:Background:The microRNAs (miRNAs) have been validated as prognostic markers in many cancers. Here, we aimed at developing a miRNA-based signature for predicting the prognosis of esophagus adenocarcinoma (EAC). Methods:The RNA-sequencing data set of EAC was downloaded from The Cancer Genome Atlas (TCGA). Eighty-four pa...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7353

    authors: Lan T,Lu Y,Xiao Z,Xu H,He J,Hu Z,Mao W

    更新日期:2019-07-25 00:00:00

  • Alu elements in primates are preferentially lost from areas of high GC content.

    abstract::The currently-accepted dogma when analysing human Alu transposable elements is that 'young' Alu elements are found in low GC regions and 'old' Alus in high GC regions. The correlation between high GC regions and high gene frequency regions make this observation particularly difficult to explain. Although a number of s...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.78

    authors: Hellen EH,Brookfield JF

    更新日期:2013-05-21 00:00:00

  • Redescription of Phymolepis  cuifengshanensis (Antiarcha: Yunnanolepididae) using high-resolution computed tomography and new insights into anatomical details of the endocranium in antiarchs.

    abstract:Background:Yunnanolepidoids constitute either the most basal consecutive segments or the most primitive clade of antiarchs, a highly diversified jawed vertebrate group from the Silurian and Early Devonian periods. Although the general morphology of yunnanolepidoids is well established, their endocranial features remain...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4808

    authors: Wang Y,Zhu M

    更新日期:2018-05-28 00:00:00

  • Necessity of electrically conductive pili for methanogenesis with magnetite stimulation.

    abstract:Background:Magnetite-mediated direct interspecies electron transfer (DIET) between Geobacter and Methanosarcina species is increasingly being invoked to explain magnetite stimulation of methane production in anaerobic soils and sediments. Although magnetite-mediated DIET has been documented in defined co-cultures reduc...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4541

    authors: Wang O,Zheng S,Wang B,Wang W,Liu F

    更新日期:2018-03-21 00:00:00

  • Study of marsh wetland landscape pattern evolution on the Zoigê Plateau due to natural/human dual-effects.

    abstract::Zoigê Plateau, China's largest plateau marsh wetland, has experienced large-scale degradation of the marsh wetland and evolution of the wetland landscape pattern over the past 40 years due to climate warming and human activities. How exactly do the wetland landscape pattern characteristics change? How do climatic chan...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9904

    authors: Dong L,Yang W,Zhang K,Zhen S,Cheng X,Wu L

    更新日期:2020-09-29 00:00:00

  • Trajectories of change after a health-education program in Japan: decay of impact in anxiety, depression, and patient-physician communication.

    abstract:Background:Health education can benefit people with chronic diseases. However, in previous research those benefits were small, and reinforcement to maintain them was not effective. A possible explanation is that the benefits appeared to be small and reinforcement appeared to be ineffective because those analyses mixed ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7229

    authors: Park MJ,Green J,Jung HS,Park YS

    更新日期:2019-07-15 00:00:00

  • Human feeding biomechanics: performance, variation, and functional constraints.

    abstract::The evolution of the modern human (Homo sapiens) cranium is characterized by a reduction in the size of the feeding system, including reductions in the size of the facial skeleton, postcanine teeth, and the muscles involved in biting and chewing. The conventional view hypothesizes that gracilization of the human feedi...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2242

    authors: Ledogar JA,Dechow PC,Wang Q,Gharpure PH,Gordon AD,Baab KL,Smith AL,Weber GW,Grosse IR,Ross CF,Richmond BG,Wright BW,Byron C,Wroe S,Strait DS

    更新日期:2016-07-26 00:00:00

  • Effectiveness of adaptive silverware on range of motion of the hand.

    abstract::Background. Hand function is essential to a person's self-efficacy and greatly affects quality of life. Adapted utensils with handles of increased diameters have historically been used to assist individuals with arthritis or other hand disabilities for feeding, and other related activities of daily living. To date, mi...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1667

    authors: McDonald SS,Levine D,Richards J,Aguilar L

    更新日期:2016-02-15 00:00:00

  • Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum L. plants cultured in RITA® bioreactor.

    abstract:Background:Leucojum aestivum L. is an important medicinal plant which produces Amaryllidaceae alkaloids, especially galanthamine and lycorine. Research is currently exploring the possibility of producing these alkaloids using biotechnological methods, including in vitro cultures. The biosynthesis of alkaloids may be af...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8688

    authors: Ptak A,Morańska E,Skrzypek E,Warchoł M,Spina R,Laurain-Mattar D,Simlat M

    更新日期:2020-03-16 00:00:00

  • Identification of viruses infecting six plum cultivars in Korea by RNA-sequencing.

    abstract:Background:Plums are a kind of stone fruit, a category that includes peaches, cherries, apricots, and almonds. In Korea, Japanese plum trees are usually cultivated as they best suit the climate. To date, there have been few studies in Korea on viruses infecting plum trees compared to those infecting peach trees. Metho...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9588

    authors: Jo Y,Choi H,Lian S,Cho JK,Chu H,Cho WK

    更新日期:2020-07-29 00:00:00