Abstract:
:Intervertebral disc degeneration (IDD) is considered to be the main cause of many spinal disorders; however, its underlying pathophysiology is not clearly understood. Recent studies indicate that excessive mechanical loading may serve a major role in the initiation of IDD. The aim of the present study was to explore the effect of noninvasive cumulative axial loading on the intervertebral discs of the lumbar spine using a novel rabbit model. Rabbits in the experimental group were placed into individual tubes specifically designed to force maintenance of an upright posture and were loaded with a heavy collar to increase the intradiscal pressure of their lumbar spine. Radiograph imaging and magnetic resonance imaging (MRI) was performed every 4 weeks to provide evidence of disc degeneration. At the end of the experiment, the animals were sacrificed and disc specimens were harvested for quantitative polymerase chain reaction and histological analysis. MRI results revealed significant and progressive reductions in the signal intensities of lumbar discs in the experimental group compared with the control group throughout the 14-week study period. The expression level of type I collagen was significantly increased and the expression levels of type II collagen and aggrecan were significantly decreased in the experimental group compared with the control group (P<0.05). Histological examination revealed marked structural changes in the experimental group, including fibrocartilage-like tissue ingrowth and accelerated fibrotic changes of the nucleus pulposus. The results of the present study indicate that noninvasive cumulative axial load is able to induce accelerated degenerative changes in rabbit lumbar discs, which may provide useful information for the establishment of a novel animal model of IDD for the research of IDD in humans.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Bai X,Wang D,Zhou M,Xu C,Li W,Tao H,He Q,Ruan Ddoi
10.3892/etm.2017.4148subject
Has Abstractpub_date
2017-04-01 00:00:00pages
1438-1446issue
4eissn
1792-0981issn
1792-1015pii
ETM-0-0-4148journal_volume
13pub_type
杂志文章abstract::The aim of this study was to isolate and purify Rhizoma Pinelliae trypsin inhibitor (RPTI), determine its N-terminal amino acid sequence and evaluate its inhibitory effect on the proliferation of poorly differentiated BGC-823 human gastric adenocarcinoma cells. RPTI was separated and purified from a 40% (NH4)2SO4 prec...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2014.1701
更新日期:2014-07-01 00:00:00
abstract::Mutations in keratin 5 (KRT5) or KRT14 genes are responsible for the most severe form of epidermolysis bullosa simplex (EBS), which is EBS generalized severe (EBS-gen sev). To date, only four pathogenic mutations (p.Arg165Ser and p.Lys199Asn in KRT5; p.Arg125Cys and p.Arg125His in KRT14) have been reported to be respo...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2016.3717
更新日期:2016-11-01 00:00:00
abstract::Chemotherapy-induced anorexia (CIA), which may lead to severe nutrition-associated problems, is a common complication associated with anti-cancer therapies. In the present study, the anti-anorexigenic effect of electroacupuncture (EA) was explored through assessing a change in appetite-associated peptides and c-Fos ex...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.7250
更新日期:2019-04-01 00:00:00
abstract::Honokiol, a potent radical scavenger, has been demonstrated to ameliorate cerebral infarction following ischemia/reperfusion (I/R) injury. However, its effects on myocardial I/R injury remain unclear. The present study aimed to examine the effects of honokiol on myocardial I/R injury and to investigate its potential c...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2012.766
更新日期:2013-01-01 00:00:00
abstract::The activation of tunica albuginea myofibroblasts (MFs) serves an essential role in Peyronie's disease (PD). Increasing evidence has reported that adipose tissue-derived stem cells (ADSCs) have been demonstrated to attenuate the symptoms of PD in animal models. However, the mechanisms of the antifibrotic effects of AD...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.5179
更新日期:2017-11-01 00:00:00
abstract::Point mutations in oncogenes and tumor suppressor genes occur at early stages in the carcinogenic process. Point mutations in ras family oncogenes are the most common mutational events in several types of human cancer, and are available as molecular markers for the detection of cancer cells in carcinogenicity bioassay...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm_00000103
更新日期:2010-07-01 00:00:00
abstract::The present study evaluated the association between different Mycoplasma pneumoniae (M. pneumonia) genotypes and clinical features of pediatric patients. Subjects were children diagnosed with community-acquired pneumonia at the Children's Hospital of Soochow University (Suzhou, China) from January 2012 to December 201...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.4721
更新日期:2017-09-01 00:00:00
abstract::Dimethyl carbonate (DMC) is a widely used industrial chemical, which may be increasingly used in the future. However, its toxicity profile remains largely unknown. The present study was designed to investigate the effects of DMC exposure on the ovaries and the effect of autophagy activation on follicular development. ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.5328
更新日期:2017-12-01 00:00:00
abstract::Studies performed previously have indicated that long non-coding RNAs (lncRNAs) may be involved in skeletal muscle regeneration; however, the roles of lncRNAs during the repair of skeletal muscle contusion remain unclear. The present study established a mouse skeletal muscle contusion injury model to identify the role...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.7871
更新日期:2019-10-01 00:00:00
abstract::Giant aneurysm of the posterior circulation is associated with a higher risk of rupture compared with that of the anterior circulation. Furthermore, surgical clipping and interventional embolization for giant aneurysm of the posterior circulation are more difficult and complex to perform. The present study reported on...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2020.9472
更新日期:2021-01-01 00:00:00
abstract::In the present study, the predictive value of glycosylated hemoglobin (HbA1c), microalbuminuria (24 h mAlb) and serum cystatin C (Cys-C) levels on the outcome of pregnancy in patients with gestational diabetes mellitus (GDM) was investigated. Samples of 144 females with GDM and 117 normal pregnant females as controls ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.8336
更新日期:2020-02-01 00:00:00
abstract::Chronic rhinosinusitis (CRS) is a heterogeneous disease, with varying immunological and histopathological features. The CC chemokine ligand 19 (CCL19) can stimulate T cells and antigen-presenting cells into secondary lymphoid node formation, as observed in allergic rhinitis, inflammatory bowel disease and other inflam...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2015.2897
更新日期:2016-01-01 00:00:00
abstract::Impaired glucose tolerance (IGT) is a pre-diabetic metabolic state involving heterogeneous and dynamic changes between the normal and diabetic state. The present study aimed to investigate the endocrine regulation of endothelium-dependent dysfunction in middle-aged patients with IGT and in patients with a normal gluco...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2014.1482
更新日期:2014-03-01 00:00:00
abstract::Corneal astigmatism significantly compromises uncorrected visual acuity (UCVA) after phacoemulsification with implantation of traditional spherical or non-spherical monofocal intraocular lens (IOL). Toric IOL provides an effective way to gain favorable postoperative UCVA for the patients with cataracts with corneal as...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2020.9153
更新日期:2020-11-01 00:00:00
abstract::The present study aimed to investigate the expression of complement component 1, q subcomponent-binding protein (C1QBP) in colon cancer cells, and identify proteins that interact with C1QBP. Total proteins were extracted from both the tumor and normal tissues of 22 patients with colon cancer and analyzed using liquid ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.4249
更新日期:2017-05-01 00:00:00
abstract::The aim of the present study was to investigate the feasibility of using composite scaffolds of poly(lactic-co-glycolic acid) (PLGA) and bioactive glass (BG) to repair bone defects. PLGA/BG composite scaffolds were prepared by thermally-induced phase separation. Scanning electron microscopy (SEM) was used to study the...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.8121
更新日期:2019-12-01 00:00:00
abstract::The aim of the study was to investigate the effects of high-volume continuous renal replacement therapy (HV-CRRT) on procalcitonin (PCT), tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), IL-6, IL-8 and IL-10 in acute pancreatitis complicated by acute renal failure. Eighty-six patients with acute pancreatitis com...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.4843
更新日期:2017-10-01 00:00:00
abstract::Neuregulin-1 (NRG-1) is a cardioactive growth factor released from endothelial cells. However, the effect of NRG-1 on ventricular remodeling in diabetic cardiomyopathy (DCM) remains unclear. The aim of the present study was to investigate the pathophysiological role of NRG-1 in a rat model of DCM. Rat cardiac microvas...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2013.1273
更新日期:2013-11-01 00:00:00
abstract::The aim of the present study was to investigate the application of 3D printing (3DP) rapid prototyping (RP) technique-assisted percutaneous fixation in the treatment of femoral intertrochanteric fracture (ITF) using proximal femoral nail anti-rotation (PFNA). A total of 39 patients with unstable ITF were included in t...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.4991
更新日期:2017-10-01 00:00:00
abstract::The new enzyme-targeting radiosensitization treatment, Kochi Oxydol-Radiation Therapy for Unresectable Carcinomas, type II (KORTUC II), markedly enhances the radiotherapeutic effect of treatment for various types of locally advanced malignant neoplasms. Patients who had declined surgical treatment and systemic chemoth...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2010.123
更新日期:2010-09-01 00:00:00
abstract::Pulmonary arterial hypertension (PAH) is a progressive disease with a high mortality rate. Previous studies have revealed the important function of the β3 adrenergic receptor (β3-AR) in cardiovascular diseases, and the potential beneficial effects of numerous β3-AR agonists on pulmonary vasodilation. Conversely, a num...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.8236
更新日期:2020-01-01 00:00:00
abstract::Engineered nanoparticles (NPs) have been increasingly used in numerous fields over the last decade. In particular, iron oxide NPs (Fe2O3NPs) and silver NPs (AgNPs) have contributed to the current increase in NP usage. However, the possible side effects of increased NP exposure remain not fully elucidated. The present ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.8108
更新日期:2019-12-01 00:00:00
abstract::Pulmonary embolism may escape prompt diagnosis since clinical symptoms and signs are nonspecific. The occurrence of syncope as the sole initial symptom in a previously healthy patient with no predisposing factors to embolism and no hemodynamic instability is extremely rare, which may have been a factor in the delayed ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2013.992
更新日期:2013-05-01 00:00:00
abstract::Nerve growth factor (NGF) and dendritic cells (DCs) have been hypothesized to modulate T cell responses in a mouse model of asthma. However, whether NGF plays a role in regulating the maturation and polarization of lung DCs remains unclear. In the present study, the effect of NGF inhibition on the maturation and pheno...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2014.1967
更新日期:2014-11-01 00:00:00
abstract::Currently available antiepileptic drugs are effective; however, frequently associated with adverse effects that limit their therapeutic value. Compounds that target the molecular events underlying epilepsy, with minor or no adverse effects, would be of clinical value. Matrix metalloproteinase-9 (MMP-9) and the brain-d...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.7282
更新日期:2019-04-01 00:00:00
abstract::Tumor-infiltrating lymphocytes (TILs) are produced by resecting tumor tissue and growing and expanding ex vivo large quantities of autologous T cells. Once the TILs are ready for infusion, the patient undergoes a non-myeloablative lympho-depleting course of chemotherapy and subsequent TIL infusion with high-dose bolus...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2012.498
更新日期:2012-05-01 00:00:00
abstract::High-mobility group box 1 (HMGB1) is released after focal cerebral ischemia/reperfusion (I/R), and aggravates brain tissue damage. Ginsenoside Rb1 (Rb1), isolated from Panax ginseng, has been reported to inhibit I/R-induced cell death in the brain. The present study aimed to investigate the protective ability of GRb1 ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6523
更新日期:2018-10-01 00:00:00
abstract::Endometriosis is a common reproductive disorder in women, with a global prevalence of 10-15%. Long noncoding RNAs (lncRNAs) are critical to gene transcription, cell cycle modulation and immune response. The lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) reportedly mediates autophagy of endometr...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2020.9453
更新日期:2021-01-01 00:00:00
abstract::To suppress the invasion of hepatocellular carcinoma (HCC) cells into surrounding connective tissues during metastasis, we investigated the usefulness of sorafenib. In order to search for model cell lines, cell numbers were counted to reveal cell lines with the potential to proliferate in serum-free media. Cell prolif...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2010.131
更新日期:2010-09-01 00:00:00
abstract::Radiation pneumonitis is a clinical problem with a high incidence. Once the onset of radiation pneumonitis has occurred, the administration of antioxidants and anti-inflammatory agents is the most commonly used method of clinical treatment. Quercetin (Que) is a common flavonoid, with potent anti-inflammatory and anti-...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.5290
更新日期:2017-12-01 00:00:00