Sensitive quantitative assay for point mutations in the rat H-ras gene based on single nucleotide primer extension.

abstract：:The CC chemokine receptor 9 (CCR9) and its natural secreted ligand CC motif chemokine ligand 25 (CCL25) have been implicated in cancer metastasis. However, their metastatic potential in non-small cell lung cancer (NSCLC) remains unclear. In the present study, immunohistochemistry was used to detect the expression and ...

journal_title：Experimental and therapeutic medicine

authors： Niu Y,Tang D,Fan L,Gao W,Lin H

更新日期：2020-06-01 00:00:00

abstract：:microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have aberrant expression in a number of tumor types. miR-192, -194 and -215 have not been comprehensively investigated using a large number of cases in colorectal cancer (CRC). We extracted total RNA from 107 CRC t...

journal_title：Experimental and therapeutic medicine

authors： Chiang Y,Song Y,Wang Z,Liu Z,Gao P,Liang J,Zhu J,Xing C,Xu H

更新日期：2012-03-01 00:00:00

abstract：:Tools to define the active ingredients and flavors of Traditional Chinese Medicines (TCMs) are limited by long analysis times, complex sample preparation and a lack of multiplexed analysis. The aim of the present study was to optimize and validate an electronic tongue (E-tongue) methodology to analyze the bitterness o...

journal_title：Experimental and therapeutic medicine

authors： Li X,Gao X,Liu R,Wang J,Wu Z,Zhang L,Li H,Gui X,Kang B,Shi J

更新日期：2016-11-01 00:00:00

abstract：:It is hypothesized that there may be common characteristics between the genetic regulatory networks of different diseases. To identify these potential similarities, analysis of overlapping differentially expressed genes (DEGs) in several diseases, which are believed to be associated in traditional Chinese medicine (TC...

journal_title：Experimental and therapeutic medicine

authors： Tang H,Zhang Y

更新日期：2018-06-01 00:00:00

abstract：:A severe immune response in patients with coronavirus disease 2019 (COVID-19) can cause a potentially lethal unconstrained inflammatory cytokine storm, known as cytokine release syndrome (CRS). The present study provides an overview of the biology underlying CRS and how targeted inhibition of interleukin (IL)-6 signal...

journal_title：Experimental and therapeutic medicine

authors： Chen JJ,Zhang LN,Hou H,Xu L,Ji K

更新日期：2021-01-01 00:00:00

abstract：:The aim of the present study was to investigate the changes in mitotic reorientation and relative differential gene expression in rat prostate epithelial cells following long-term exposure to testosterone propionate (TP). Sprague-Dawley rats were randomly divided into two groups as follows: TP group, which received 3....

journal_title：Experimental and therapeutic medicine

authors： Liu X,Cheng YI,Pan QI,Hu W,Xu LI,Meng X,Wu J,Xie C,Yan H,Sun Z

更新日期：2016-04-01 00:00:00

abstract：:Malignant melanoma is the most aggressive neoplasm, with severe metastatic potential. microRNAs represent a class of endogenously expressed, small non-coding RNAs that regulate gene expression. As a consequence, the translation of these mRNAs is inhibited or they are destabilized resulting in downregulation of the enc...

journal_title：Experimental and therapeutic medicine

authors： Yamazaki H,Chijiwa T,Inoue Y,Abe Y,Suemizu H,Kawai K,Wakui M,Furukawa D,Mukai M,Kuwao S,Saegusa M,Nakamura M

更新日期：2012-05-01 00:00:00

abstract：:The present study performed a meta-analysis of randomized controlled trials in multiple sclerosis (MS) patients to evaluate the efficacy and safety of anti-B-cell monoclonal antibodies (mAbs). To the best of our knowledge, no previous meta-analysis has evaluated this. Relevant studies published until March 2015 were r...

journal_title：Experimental and therapeutic medicine

authors： Xie Q,Li X,Sun J,Yuan B,Li Y,Wang L,Wang M

更新日期：2017-06-01 00:00:00

abstract：:The US Food and Drug Administration approved thalidomide and its analogues for the treatment of erythema nodosum leprosum, in spite of the notoriety of reports of severe birth defects in the middle of the last century. As immunomodulatory drugs, thalidomide and its analogues have been used to effectively treat various...

journal_title：Experimental and therapeutic medicine

authors： Liu T,Guo F,Zhu X,He X,Xie L

更新日期：2017-12-01 00:00:00

abstract：:Clinical efficacy of montelukast sodium combined with budesonide or combined with loratadine in children with cough variant asthma was investigated. A retrospective analysis of the medical records of 72 children with cough variant asthma who were treated in Xuzhou Children's Hospital, Xuzhou Medical University from Ap...

journal_title：Experimental and therapeutic medicine

authors： Wei H,Li W,Jiang Z,Xi X,Qi G

更新日期：2019-07-01 00:00:00

abstract：:Mevalonate kinase (MVK) mutations were previously identified in disseminated superficial actinic porokeratosis. However, the role of MVK in differentiation, apoptosis and prenylation of keratinocytes requires further investigation. Farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP) of the mevalonate ...

journal_title：Experimental and therapeutic medicine

authors： Li M,Min W,Wang J,Wang L,Li Y,Zhou N,Yang Z,Qian Q

更新日期：2020-04-01 00:00:00

abstract：:The present study aimed to analyze the imaging, clinical and pathological features of fungal infection involvement in primary biliary cirrhosis (PBC) by retrospectively analyzing and reviewing the features of two patients with fungal infection involvement in PBC. Both patients were female. One patient had a confirmed ...

journal_title：Experimental and therapeutic medicine

authors： Wang Y,Zhao Z,Lu H,Zhang J,Huang F

更新日期：2017-02-01 00:00:00

abstract：:Conjunctivitis associated systemic lupus erythematosus (caSLE) is a connective tissue autoimmune disease with a large spectrum of clinical manifestations. The disease may lead to ocular complications and in severe cases may be sight-threatening. This study investigated the expression of vascular adhesion protein-1 (VA...

journal_title：Experimental and therapeutic medicine

authors： Liu H,Zhang J,Zhou P,Sun H,Katsarou M,Drakoulis N

更新日期：2019-12-01 00:00:00

abstract：:17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG) is a water soluble, semisynthetic derivative of endotoxin that has anticancer effects. The aim of the present study was to determine whether 17-DMAG enhances the apoptosis of lymphoma cells in diffuse large B-cell lymphoma. Apoptosis was induced in SU-DHL-4...

journal_title：Experimental and therapeutic medicine

authors： Li JJ,Zhang JJ,Wang X,Sun ZM

更新日期：2017-10-01 00:00:00

abstract：:Adipose-derived stem cells (ADSCs) possess good proliferative and differentiative abilities, making then a promising candidate for the treatment of cartilage defects. However, local ischemia often causes apoptosis in ADSCs. Transforming growth factor-β3 (TGF-β3) is often used as a chondrogenic differentiation cytokine...

journal_title：Experimental and therapeutic medicine

authors： Wu F,Ye H,Lin J,Xu Y,Zhang Z,Xiong H,Laing M,Zhen Y,Chen S

更新日期：2018-05-01 00:00:00

abstract：:Eosinophilia has been implicated in the pathophysiology of acute exacerbation of chronic obstructive pulmonary disease (AECOPD). However, the role of eosinophil activation in the development of AECOPD remains unclear. In the present study, the reliability of plasma levels of eosinophil activation markers, including eo...

journal_title：Experimental and therapeutic medicine

authors： Yang QF,Lu TT,Shu CM,Feng LF,Chang HT,Ji QY

更新日期：2017-10-01 00:00:00

abstract：:A mutation in the IIb sodium phosphate transporter SLC34A2 gene has recently been described in pulmonary alveolar microlithiasis (PAM) patients. Experiments in this study were aimed at confirming the role of the gene product in PAM by comparing phosphorylated products in extracellular fluid of alveolar epithelial cell...

journal_title：Experimental and therapeutic medicine

authors： Ma T,Qu D,Yan B,Zhang Q,Ren J,Hu Y

更新日期：2018-01-01 00:00:00

abstract：:Jiawei Shenqi-wan (JSQW), which comprises Shenqi-wan and two additional medicinal herbs, has been widely used for the treatment of various growth impairments, including cerebral palsy. In the present study, JSQW was administered to hypoxic-ischemic Sprague-Dawley rats that underwent treadmill training from 4-7 weeks o...

journal_title：Experimental and therapeutic medicine

authors： Kim HN,Pak ME,Shin MJ,Kim SY,Shin YB,Yun YJ,Shin HK,Choi BT

更新日期：2017-05-01 00:00:00

abstract：:The aim of the present meta-analysis was to systematically assess the efficacy of the various treatments available for moderate to severe psoriasis. PubMed and Embase databases were systematically searched to select relevant studies up to February 2015. Odds ratios (ORs) and their 95% confidence intervals (CIs) were u...

journal_title：Experimental and therapeutic medicine

authors： Geng W,Zhao J,Fu J,Zhang H,Qiao S

更新日期：2018-12-01 00:00:00

abstract：:Ocimum sanctum L. (OS) leaves have been shown to exert diverse potential benefits in a variety of stress conditions. The present study was conducted to elucidate the effects of the fixed oil extracted from OS leaves on the blood glucose levels and serum lipid profile of streptozotocin-induced diabetic rats. In additio...

journal_title：Experimental and therapeutic medicine

authors： Suanarunsawat T,Anantasomboon G,Piewbang C

更新日期：2016-03-01 00:00:00

abstract：:MicroRNAs (miRs), a class of small non-coding RNAs, function as key regulators in gene expression through binding to the 3'-untranslated region (UTR) of their target mRNA, which further leads to translational repression or mRNA degradation. Recently, miR-138 has been found to have a tumor suppressive role in a variety...

journal_title：Experimental and therapeutic medicine

authors： Yuan Z,Mo H,Mo L,He J,Wu Z,Lin X

更新日期：2017-06-01 00:00:00

abstract：:The present study was conducted to investigate the expression of apoptosis-related factors in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B and their correlation to clinical prognosis. Sixty-two patients that were diagnosed with chronic hepatitis B were admitted to Xuzhou Hospital from...

journal_title：Experimental and therapeutic medicine

authors： Guo L,Liang Y

更新日期：2017-12-01 00:00:00

abstract：:Gastric carcinoma is one of the most common human cancers and has a poor prognosis. Receptor tyrosine kinase-like orphan receptor 2 (ROR2), which is a non-canonical receptor of the Wnt signaling pathway, has been reported to be deregulated in numerous types of human cancers, including gastric carcinoma. However, the e...

journal_title：Experimental and therapeutic medicine

authors： Yan L,Du Q,Yao J,Liu R

更新日期：2016-12-01 00:00:00

abstract：:Lysyl oxidase proteins (LOXs) are amine oxidases, which are mainly located in smooth muscle cells and fibroblasts and serve an important role in the formation of the extracellular matrix (ECM) in a copper-dependent manner. Owing to the ability of LOX proteins to modulate crosslinking between collagens and to promote t...

journal_title：Experimental and therapeutic medicine

authors： Wei S,Gao L,Wu C,Qin F,Yuan J

更新日期：2020-07-01 00:00:00

abstract：:The aim of the present study was to investigate whether the co-injection of hyaluronic acid (HA) and corticosteroids (CS) was superior to HA alone in the treatment of knee OA. A total of 120 participants with symptomatic knee OA were recruited and formed the intention-to-treat population for a 6-month follow-up. In th...

journal_title：Experimental and therapeutic medicine

authors： Wang SZ,Wu DY,Chang Q,Guo YD,Wang C,Fan WM

更新日期：2018-09-01 00:00:00

abstract：:The aim of the present study was to evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia inducible factor-1 (HIF-1), and to investigate the role of the HIF-1/VEGF signaling pathway following spinal cord injury (SCI). A total of 90 12-week-old Sprague Dawley rats were randomly divided into t...

journal_title：Experimental and therapeutic medicine

authors： Chen H,Li J,Liang S,Lin B,Peng Q,Zhao P,Cui J,Rao Y

更新日期：2017-03-01 00:00:00

abstract：:The present study aimed to determine the expression of microRNA (miRNA or miR)-145 in hypertrophic scars at the tissue and cellular levels, and to investigate its biological functions and mechanism of action. A total of 36 patients who were diagnosed with hypertrophic scar were included in the present study. Reverse t...

journal_title：Experimental and therapeutic medicine

authors： Wang S,Li C,Yu Y,Qiao J

更新日期：2019-11-01 00:00:00

abstract：:In this study, we assessed the effects of pre-pregnancy body mass index (BMI) and gestational weight gain (GWG) on the pregnancy outcomes of women of advanced age using a back-propagation (BP) artificial neural network. We conducted a retrospective analysis on postpartum and hospital delivery data from 1,015 women of ...

journal_title：Experimental and therapeutic medicine

authors： Lin J,Fu Y,Han Q,Yan J,Chen R,Zhang H

更新日期：2019-09-01 00:00:00

abstract：:Donor organ rejection remains a significant problem. The present study aimed to assess whether transferring a donor's major histocompatibility complex (MHC) genes to the recipient could mitigate rejection in organ transplantation. Seven loci of MHC genes from donor mice were amplified and ligated into vectors; the vec...

journal_title：Experimental and therapeutic medicine

authors： Li T,Zhang W,Xu Q,Li S,Tong X,Ding J,Li H,Hou S,Xu Z,Jablons DM,You L

更新日期：2018-06-01 00:00:00

abstract：:Recurrent patellar dislocations were treated via knee arthroscopy combined with C-arm fluoroscopy, and reconstruction of the medial patellofemoral ligaments. Between October 2013 and March 2017, 52 cases of recurrent patellar dislocation [27 males and 25 females; age, 16-47 years (mean, 21.90 years)] were treated. Art...

journal_title：Experimental and therapeutic medicine

authors： Li L,Wang H,He Y,Si Y,Zhou H,Wang X

更新日期：2018-06-01 00:00:00