Abstract:
:Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms. Furthermore, we describe hotspots at which the guideRNA itself is edited and show a way to circumvent this auto-editing without losing editing efficiency at the target. Both findings are important for the advancement of site-directed RNA editing as a tool in basic biology or as a platform for therapeutic editing.
journal_name
Genes (Basel)journal_title
Genesauthors
Heep M,Mach P,Reautschnig P,Wettengel J,Stafforst Tdoi
10.3390/genes8010034subject
Has Abstractpub_date
2017-01-14 00:00:00issue
1issn
2073-4425pii
genes8010034journal_volume
8pub_type
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