Abstract：
:Recent findings demonstrated that a subset of papillary thyroid cancers (PTCs) is characterized by reduced expression of the von Hippel-Lindau (VHL) tumor suppressor gene, and that lowest levels associated with more aggressive PTCs. In the present study, the levels of the two VHL mRNA splicing variants, VHL-213 (V1) and VHL-172 (V2), were measured in a series of 96 PTC and corresponding normal matched tissues by means of quantitative RT-PCR. Variations in the mRNA levels were correlated with patients' clinicopathological parameters and disease-free interval (DFI). The analysis of VHL mRNA in tumor tissues, compared to normal matched tissues, revealed that its expression was either up- or down-regulated in the majority of PTC. In particular, V1 and V2 mRNA levels were altered, respectively, in 78 (81.3%) and 65 (67.7%) out of the 96 PTCs analyzed. A significant positive correlation between the two mRNA variants was observed (p < 0.001). Univariate analysis documented the lack of association between each variant and clinicopathological parameters such as age, tumor size, histology, TNM stage, lymph node metastases, and BRAF mutational status. However, a strong correlation was found between altered V1 or V2 mRNA levels and DFI. Multivariate regression analysis indicated higher V1 mRNA values, along with lymph node metastases at diagnosis, as independent prognostic factors predicting DFI. In conclusion, the data reported demonstrate that VHL gene expression is deregulated in the majority of PTC tissues. Of particular interest is the apparent protective role exerted by VHL transcripts against PTC recurrences.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Baldini E,Tuccilli C,Arlot-Bonnemains Y,Chesnel F,Sorrenti S,De Vito C,Catania A,D'Armiento E,Antonelli A,Fallahi P,Watutantrige-Fernando S,Tartaglia F,Barollo S,Mian C,Bononi M,Arceri S,Mascagni D,Vergine M,Pironi Ddoi
10.1016/j.mce.2017.01.019subject
Has Abstractpub_date
2017-03-05 00:00:00pages
121-127eissn
0303-7207issn
1872-8057pii
S0303-7207(17)30023-0journal_volume
443pub_type
杂志文章abstract：:Rapid, nongenomic in vitro effects of aldosterone on intracellular electrolytes, cell volume and the sodium-proton antiporter have been found in human mononuclear leukocytes (HML), as have related membrane receptors. In the present study, binding of 125I-labeled aldosterone to plasma membrane preparations from pig kid...
journal_title：Molecular and cellular endocrinology
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journal_title：Molecular and cellular endocrinology
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