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Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.

Abstract：

:The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Macedo TJS,Santos JMS,Bezerra MÉS,Menezes VG,Gouveia BB,Barbosa LMR,Lins TLBG,Monte APO,Barberino RS,Batista AM,Barros VRP,Wischral A,Queiroz MAA,Araújo GGL,Matos MHT

doi

10.1016/j.mce.2019.110506

subject

Has Abstract

pub_date

2019-09-15 00:00:00

pages

110506

eissn

0303-7207

issn

1872-8057

pii

S0303-7207(19)30208-4

journal_volume

495

pub_type

杂志文章

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