Abstract:
OBJECTIVE:To validate a hypothesis that prostate cancer can be detected non-invasively by a simple and reliable assay by targeting genomic VPAC receptors expressed on malignant prostate cancer cells shed in voided urine. PATIENTS/SUBJECTS AND METHODS:VPAC receptors were targeted with a specific biomolecule, TP4303, developed in our laboratory. With an Institutional Review Board exempt approval of use of de-identified discarded samples, an aliquot of urine collected as a standard of care, from patients presenting to the urology clinic (207 patients, 176 men and 31 women, aged ≥21 years) was cytospun. The cells were fixed and treated with TP4303 and 4,6-diamidino-2-phenylindole (DAPI). The cells were then observed under a microscope and cells with TP4303 orange fluorescence around the blue (DAPI) nucleus were considered 'malignant' and those only with a blue nucleus were regarded as 'normal'. VPAC presence was validated using receptor blocking assay and cell malignancy was confirmed by prostate cancer gene profile examination. RESULTS:The urine specimens were labelled only with gender and presenting diagnosis, with no personal health identifiers or other clinical data. The assay detected VPAC positive cells in 98.6% of the men with a prostate cancer diagnosis (141), and none of the 10 men with benign prostatic hyperplasia. Of the 56 'normal' patients, 62.5% (35 patients, 10 men and 25 women) were negative for VPAC cells; 19.6% (11, 11 men and no women) had VPAC positive cells; and 17.8% (10, four men and six women) were uninterpretable due to excessive crystals in the urine. Although data are limited, the sensitivity of the assay was 99.3% with a confidence interval (CI) of 96.1-100% and the specificity was 100% with a CI of 69.2-100%. Receptor blocking assay and fluorescence-activated cell sorting (FACS) analyses demonstrated the presence of VPAC receptors and gene profiling examinations confirmed that the cells expressing VPAC receptors were malignant prostate cancer cells. CONCLUSION:These preliminary data are highly encouraging and warrant further evaluation of the assay to serve as a simple and reliable tool to detect prostate cancer non-invasively.
journal_name
BJU Intjournal_title
BJU internationalauthors
Trabulsi EJ,Tripathi SK,Gomella L,Solomides C,Wickstrom E,Thakur MLdoi
10.1111/bju.13775subject
Has Abstractpub_date
2017-06-01 00:00:00pages
885-895issue
6eissn
1464-4096issn
1464-410Xjournal_volume
119pub_type
杂志文章abstract:OBJECTIVE:To evaluate the long-term follow-up (primary and secondary patency) of metal stent placement in benign ureteroileal anastomotic strictures after Bricker urinary diversion and to compare the failed treatment group with the group of successfully treated patients to search for predisposing factors of stent failu...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.12729
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abstract:OBJECTIVE:To study scrotal microvascularization and apply the findings to the design of reliable skin flaps for reconstructive surgery of complex urethral or panurethral stenoses. MATERIALS AND METHODS:In 15 cryopreserved male cadavers, scrotal skin vascularization was explored using macro- and microdissections, and t...
journal_title:BJU international
pub_type: 杂志文章
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abstract:OBJECTIVES:To describe the surgical technique of transcorporal artificial urinary sphincter (AUS) implantation. To assess the efficacy of the AUS on continence and erectile function. PATIENTS AND METHODS:A prospective evaluation was conducted between December 2007 and October 2012 at a tertiary referral centre of all ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.12386
更新日期:2013-12-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.06569.x
更新日期:2007-02-01 00:00:00
abstract:OBJECTIVE:• To evaluate the safety and efficacy of once-daily trospium chloride extended release (ER) in overactive bladder syndrome (OAB) in subjects aged ≥ 75 years. SUBJECTS AND METHODS:• The analysis included subjects ≥ 75 years of age with OAB. • A subgroup analysis of pooled data was performed for subjects aged ...
journal_title:BJU international
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1464-410X.2010.09519.x
更新日期:2011-02-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.12151
更新日期:2013-08-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2008.07474.x
更新日期:2008-06-01 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:2010-03-01 00:00:00
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journal_title:BJU international
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doi:10.1046/j.1464-410x.1999.00895.x
更新日期:1999-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1464-410X.2005.05522.x
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doi:10.1046/j.1464-410x.2000.00668.x
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pub_type: 临床试验,杂志文章,多中心研究
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doi:10.1046/j.1464-410x.2003.04355.x
更新日期:2003-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00
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更新日期:2011-11-01 00:00:00
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更新日期:2008-03-01 00:00:00
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doi:10.1111/j.1464-410X.2008.07627.x
更新日期:2008-08-01 00:00:00
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更新日期:2014-12-01 00:00:00
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更新日期:2011-10-01 00:00:00
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更新日期:2005-12-01 00:00:00
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更新日期:2008-11-01 00:00:00
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journal_title:BJU international
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更新日期:2006-02-01 00:00:00
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更新日期:2011-08-01 00:00:00