Abstract:
OBJECTIVE:To investigate the feasibility of transcript profiling in diagnostic formalin-fixed and paraffin-embedded (FFPE) biopsies for prostate cancer. MATERIALS AND METHODS:Laser-capture microdissection (LCM) was used to microdissect glandular epithelium as well as stromal tissue in archival prostate needle biopsies. Optimized RNA extraction, reverse transcription and real-time PCR (QPCR) protocols were used to detect transcript expression. RNA degradation effects were assessed using hydrolysed cell line RNA and matched xenograft FFPE and frozen tumours. RESULTS:LCM and RNA extraction was achieved in all biopsies from a pilot cohort of five patients. cDNA produced was successfully used to detect expression of glyceraldehyde-3-phosphate dehydrogenase, RPL13, prostate-specific antigen, vimentin, inhibitor of differentiation/DNA binding 1 (Id-1) and polycomb group protein enhancer of zeste homolog 2 (EZH2) transcripts. In the cell line and xenograft models, we investigated the effect of RNA degradation on transcript quantification by QPCR. In both models normalization of transcript quantity with a housekeeping gene resulted in restored expression in all degraded samples to within a 50% difference of control samples. Using an extended cohort of 29 biopsies, we tested application in detecting differences in EZH2 and Id-1 expression between malignant and benign epithelium. The results confirmed that our technique was capable of quantifying significant differences in expression between malignant and benign epithelium consistent with the reported trends. CONCLUSION:This study reports the use of standard FFPE needle biopsies for transcript profiling and supports the concept of molecular prognostic studies in tissue acquired at diagnosis in prostate cancer.
journal_name
BJU Intjournal_title
BJU internationalauthors
Rogerson L,Darby S,Jabbar T,Mathers ME,Leung HY,Robson CN,Sahadevan K,O'Toole K,Gnanapragasam VJdoi
10.1111/j.1464-410X.2008.07627.xsubject
Has Abstractpub_date
2008-08-01 00:00:00pages
364-70issue
3eissn
1464-4096issn
1464-410Xpii
BJU7627journal_volume
102pub_type
杂志文章abstract:UNLABELLED:OBJECTIVE • To determine how the presence of MP on T1 biopsy specimens affects the outcome of patients undergoing RC as compared to when no MP is identified in the TURBT specimen. PATIENTS AND METHODS:• Patients were retrospectively identified from the Columbia University Urologic Oncology Database. • From ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09893.x
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVE:To evaluate whether Raman molecular imaging (RMI, which combines digital imaging and analytical spectroscopy to evaluate the biochemical composition of interrogated material) can be used to identify biochemical differences in patients with Gleason 7 prostate cancer who progress to metastatic disease and die f...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09185.x
更新日期:2010-08-01 00:00:00
abstract:OBJECTIVE:To assess the safety and efficacy of the tension-free vaginal tape procedure in the treatment of primary genuine stress incontinence. PATIENTS AND METHODS:A two-centre follow-up study was conducted on 40 women with urodynamically confirmed primary genuine stress incontinence who had a tension-free vaginal ta...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1464-410x.2000.00731.x
更新日期:2000-07-01 00:00:00
abstract:OBJECTIVES:To examine patterns of care among women with urogenital fistula treated in the English National Health Service (NHS) between 2000 and 2009. To assess whether failure of repair was associated with hospital or surgeon workload. PATIENTS AND METHODS:We conducted a retrospective cohort study using data from Hos...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2012.11483.x
更新日期:2013-04-01 00:00:00
abstract:UNLABELLED:WHAT'S KNOWN ON THE SUBJECT? AND WHAT DOES THE STUDY ADD?: The EndoSew(®) prototype was first tested in a porcine model several years ago. The investigators found it both simple to master and reliable, its greatest advantage being a 2.4-fold time saving compared with straight laparoscopic suturing. In additi...
journal_title:BJU international
pub_type: 临床试验,杂志文章
doi:10.1111/j.1464-410X.2012.11599.x
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:To ascertain the prevalence of lower urinary tract symptoms (LUTS) and urinary incontinence (UI) in Canada, using a cross-Canada telephone survey, as there is a wide discrepancy in the reported prevalence of these conditions. SUBJECTS AND METHODS:A random survey with a standardized questionnaire was conducte...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2007.07198.x
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVE:To study guideline recommendation (GR)-concordance rates of treatment in elderly patients with urothelial carcinoma of the bladder (UCB) and to identify predictors of survival. PATIENTS AND METHODS:The records of 206 consecutive patients aged ≥ 75 years (median age 79 years; range 75-95) were reviewed. All p...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09417.x
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVE:To examine the urinary cytological changes caused by flexible cystoscopy and provide clinical guidelines for a reliable time interval for urinary cytological examination after flexible cystoscopy. PATIENTS AND METHODS:Forty-eight patients attending for flexible cystoscopy were recruited into the study. Each ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2004.04998.x
更新日期:2004-09-01 00:00:00
abstract:INTRODUCTION:To prospectively evaluate the accuracy of transvesical contrast-enhanced ultrasound (CEUS) as an alternative method for the detection of anastomotic leakage after radical retropubic prostatectomy (RRP) in comparison with the current standard method of conventional retrograde cystography (CG). PATIENTS AND...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09497.x
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVES:To validate a flexible cystoscopy simulator by determining if it could differentiate between expert and novice cystoscopists. SUBJECTS AND METHODS:Seventeen subjects (10 novices with no previous endoscopic experience and seven urologists who had all carried out > 1000 flexible cystoscopies each) were asked ...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2002.03090.x
更新日期:2002-12-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.13448
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.12333
更新日期:2014-03-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.1999.00268.x
更新日期:1999-11-01 00:00:00
abstract:OBJECTIVE:To report our experience with laparoscopic robotic-assisted management of pelvi-ureteric junction obstruction (PUJO) in patients with horseshoe kidneys. PATIENTS AND METHODS:Between January 2002 and May 2003, two men and a woman with horseshoe kidneys (mean age 44.6 years) had laparoscopic dismembered pyelop...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.05967.x
更新日期:2006-03-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2008.08337.x
更新日期:2009-06-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2002.02981.x
更新日期:2002-10-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09890.x
更新日期:2011-07-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2004.05115.x
更新日期:2004-11-01 00:00:00
abstract::Study Type - Therapy (case series) Level of Evidence 4 What's known on the subject? and What does the study add? The resection of the distal ureter and its orifice is an oncological principle during radical nephroureterectomy which is based on the fact that it represents a part of the urinary tract exposed to a consid...
journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/j.1464-410X.2010.09835.x
更新日期:2011-07-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.12075
更新日期:2013-06-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1111/bju.15105
更新日期:2020-08-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2003.04222.x
更新日期:2003-06-01 00:00:00
abstract::Renal cell carcinoma (RCC) is among the most resistant tumours to chemotherapy, radiotherapy and hormonal therapy. Cytokine therapy is effective in a small subset of patients, but it is associated with substantial toxicity and rarely benefits patients with extensive tumour burdens or adverse prognostic factors. Since ...
journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/j.1464-410X.2009.08987.x
更新日期:2009-12-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2007.07129.x
更新日期:2007-10-01 00:00:00
abstract:OBJECTIVES:To assess the activity of baclofen, a potent gamma-aminobutyric acid-ergic agonist, against dysfunctional voiding (DV) and lower urinary tract symptoms (LUTS) in a clinical trial, as DV is a leading cause of LUTS in women, but there is no effective treatment. PATIENTS AND METHODS:We conducted a randomized d...
journal_title:BJU international
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1464-410X.2007.06987.x
更新日期:2007-09-01 00:00:00
abstract:OBJECTIVES:To determine the most frequent urodynamic observations associated with bladder calculi, and to assess whether the presence of calculi alters these observations. PATIENTS AND METHODS:Fifty patients with bladder stones were included in a prospective study in which two urodynamic tests were used, one at inclus...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2004.04815.x
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the association of patient age with pathological and long-term oncological outcomes after radical cystectomy (RC) for bladder carcinoma, as this disease, like many others, increases in incidence with age. PATIENTS AND METHODS:We retrospectively reviewed 241 consecutive patients with invasive blad...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2008.08033.x
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVE:To determine the long-term results of constructing a sigmoid neobladder after radical cystectomy for transitional cell carcinoma (TCC) of the urinary bladder. PATIENTS AND METHODS:The study included 170 patients with TCC of the bladder and a normal sigmoid colon. After radical cystectomy the neobladder was f...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2006.06541.x
更新日期:2007-02-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,随机对照试验
doi:10.1111/bju.12926
更新日期:2015-05-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2010.09566.x
更新日期:2011-03-01 00:00:00