Abstract:
:An efficient and sensitive analytical method based on high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was established for the simultaneous separation and determination of glucosamine (GlcN)₁ and chitooligosaccharides (COS) ranging from (GlcN)₂ to (GlcN)₆ without prior derivatization. Detection limits were 0.003 to 0.016 mg/L (corresponding to 0.4-0.6 pmol), and the linear range was 0.2 to 10 mg/L. The optimized analysis was carried out on a CarboPac-PA100 analytical column (4 × 250 mm) using isocratic elution with 0.2 M aqueous sodium hydroxide-water mixture (10:90, v/v) as the mobile phase at a 0.4 mL/min flow rate. Regression equations revealed a good linear relationship (R² = 0.9979-0.9995, n = 7) within the test ranges. Quality parameters, including precision and accuracy, were fully validated and found to be satisfactory. The fully validated HPAEC-PAD method was readily applied for the quantification of (GlcN)1-6 in a commercial COS technical concentrate. The established method was also used to monitor the acid hydrolysis of a COS technical concentrate to ensure optimization of reaction conditions and minimization of (GlcN)₁ degradation.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Cao L,Wu J,Li X,Zheng L,Wu M,Liu P,Huang Qdoi
10.3390/ijms17101699subject
Has Abstractpub_date
2016-10-10 00:00:00issue
10issn
1422-0067pii
ijms17101699journal_volume
17pub_type
杂志文章abstract::In the first trimester of pregnancy, placental development involves a wide range of cellular processes. These include trophoblast proliferation, fusion, and differentiation, which are dependent on tight cell cycle control. The intrauterine environment affects placental development, which also includes the trophoblast ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020468
更新日期:2020-01-11 00:00:00
abstract::Ionic liquids (ILs) are chemical compounds composed of ions with melting points below 100 °C exhibiting a design feature. ILs are commonly used as the so-called green solvents, reagents or highly efficient catalysts in varied chemical processes. The huge application potential of ionic liquids (IL) justifies the growin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21124253
更新日期:2020-06-15 00:00:00
abstract::This work investigated in vitro aggregation and amyloid properties of skeletal myosin binding protein-C (sMyBP-C) interacting in vivo with proteins of thick and thin filaments in the sarcomeric A-disc. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) found a rapid (5-10 min) formation of large...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22020731
更新日期:2021-01-13 00:00:00
abstract::Prodrug activator gene therapy mediated by murine leukemia virus (MLV)-based retroviral replicating vectors (RRV) was previously shown to be highly effective in killing glioma cells both in culture and in vivo. To avoid receptor interference and enable dual vector co-infection with MLV-RRV, we have developed another R...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041433
更新日期:2020-02-20 00:00:00
abstract::Oxidative cellular damage caused by free radicals is known to contribute to the pathogenesis of various diseases such as cancer, diabetes, and neurodegenerative diseases, as well as to aging. The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associated protein1 (Keap1) sign...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19010026
更新日期:2017-12-22 00:00:00
abstract::Multinucleated osteoclasts differentiate from hematopoietic progenitors of the monocyte/macrophage lineage. Because of its pivotal role in bone resorption, regulation of osteoclast differentiation is a potential therapeutic approach to the treatment of erosive bone disease. In this study, we have found that fucoidan, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151018840
更新日期:2014-10-20 00:00:00
abstract::Retinoids exert antitumor effects through the retinoic acid receptor α (RARα). In the present study, we sought to identify the factors involved in the RARα-mediated transcriptional regulation of the tumor suppressor gene and the tissue factor pathway inhibitor 2 (TFPI2) in hepatocellular carcinoma (H...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19051450
更新日期:2018-05-13 00:00:00
abstract::The surface attachment properties of the Creutz-Taube ion, i.e., [(NH(3))(5)Ru(pyrazine)Ru(NH(3))(5)](5+), on both hydrophilic and hydrophobic types of surfaces were investigated using X-ray photoelectron spectroscopy (XPS). The results indicated that the Creutz-Taube ions only bound to hydrophilic surfaces, such as S...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms10020533
更新日期:2009-02-01 00:00:00
abstract::Due to the addressability and programmability, DNA has been applied not merely in constructing static elegant nanostructures such as two dimensional and three dimensional DNA nanostructures but also in designing dynamic nanodevices. Moreover, DNA could combine with hydrophobic organic molecules to be a new amphiphilic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19082283
更新日期:2018-08-03 00:00:00
abstract::Extracellular acidification occurs under physiologic and pathologic conditions, such as exercise, ischemia, and inflammation. It has been shown that acidosis has various adverse effects on bone. In recent years there has been increasing evidence which indicates that ovarian cancer G protein-coupled receptor 1 (OGR1) i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms151222365
更新日期:2014-12-03 00:00:00
abstract::RNA binding protein (RBPs) and microRNAs (miRNAs or miRs) are post-transcriptional regulators of gene expression that are implicated in development of cancers. Although their individual roles have been studied, the crosstalk between RBPs and miRNAs is under intense investigation. Here, we show that in breast cancer ce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16047112
更新日期:2015-03-30 00:00:00
abstract::Melanopsin-containing retinal ganglion cells (mRGCs) represent a third class of retinal photoreceptors involved in regulating the pupillary light reflex and circadian photoentrainment, among other things. The functional integrity of the circadian system and melanopsin cells is an essential component of well-being and ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20133164
更新日期:2019-06-28 00:00:00
abstract::Reduced low-density lipoprotein (LDL) cholesterol level is a characteristic feature of dyslipidemia in chronic hepatitis C virus (HCV) infection. However, abnormality in serum triglyceride (TG) has not been fully investigated. To clarify the impact of HCV genotype 1b (G1b) infection and advanced fibrosis on serum TG p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160920576
更新日期:2015-08-31 00:00:00
abstract::Long non-coding RNAs (lncRNAs) play crucial roles in diverse biological processes and human complex diseases. Distinguishing lncRNAs from protein-coding transcripts is a fundamental step for analyzing the lncRNA functional mechanism. However, the experimental identification of lncRNAs is expensive and time-consuming. ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21155222
更新日期:2020-07-23 00:00:00
abstract::Pigment nephropathy is an acute decline in renal function following the deposition of endogenous haem-containing proteins in the kidneys. Haem pigments such as myoglobin and haemoglobin are filtered by glomeruli and absorbed by the proximal tubules. They cause renal vasoconstriction, tubular obstruction, increased oxi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20081997
更新日期:2019-04-23 00:00:00
abstract::(R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western bl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13032717
更新日期:2012-01-01 00:00:00
abstract::3,3'-Diindolylmethane (DIM) is a naturally derived chemopreventive compound. It comes from glucobrassicin, an indole glucosinolate enriched in cruciferous vegetables, and is formed in the acidic environment of the stomach after ingestion. Mouse double minute 2 homolog (MDM2) is an important, multi-functional oncogenic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134642
更新日期:2020-06-30 00:00:00
abstract::Hibernation is an effective energy conservation strategy that has been widely adopted by animals to cope with unpredictable environmental conditions. The liver, in particular, plays an important role in adaptive metabolic adjustment during hibernation. Mammalian studies have revealed that many genes involved in metabo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19082363
更新日期:2018-08-10 00:00:00
abstract::Despite the low mortality rates, well-differentiated thyroid carcinomas (WDTC) frequently relapse. BRAF and TERT mutations have been extensively related to prognosis in thyroid cancer. In this study, the methylation levels of selected CpGs (5-cytosine-phosphate-guanine-3) comprising a classifier, previously reported b...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20061334
更新日期:2019-03-16 00:00:00
abstract::MicroRNAs (miRNAs) play important roles in the progression of human cancer. Although previous reports have shown that miR-145-5p is down-regulated in esophageal squamous cell carcinoma (ESCC), the roles and mechanisms of down-regulation of miR-145-5p in ESCC are still largely unknown. Using microRNA microarray and Gen...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18091833
更新日期:2017-08-23 00:00:00
abstract::Chlorpyrifos (CPF) is widely used in the prevention and control of crop pests and diseases in agriculture. However, the irrational utilization of pesticides not only causes environmental pollution but also threatens human health. Compared with the conventional techniques for the determination of pesticides in soil, su...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20112817
更新日期:2019-06-10 00:00:00
abstract::The endo-β-1,4-glucanase gene celE from the anaerobic fungus Orpinomyces PC-2 was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPIC9K, and integrated into the genome of a methylotrophic yeast P. pastoris GS115 by electroporation. The strain with highest endo-β-1,4-glucanase activity wa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12053366
更新日期:2011-01-01 00:00:00
abstract::The rapid rise of obesity during the past decades has coincided with a profound shift of our living environment, including unhealthy dietary patterns, a sedentary lifestyle, and physical inactivity. Genetic predisposition to obesity may have interacted with such an obesogenic environment in determining the obesity epi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18040787
更新日期:2017-04-07 00:00:00
abstract::The dysregulation of macrophage lipid metabolism drives atherosclerosis. AMP-activated protein kinase (AMPK) is a master regulator of cellular energetics and plays essential roles regulating macrophage lipid dynamics. Here, we investigated the consequences of atherogenic lipoprotein-induced foam cell formation on down...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21239033
更新日期:2020-11-27 00:00:00
abstract::Zinc oxide nanoparticles (ZnO NPs) have shown adverse health impact on the human male reproductive system, with evidence of inducing apoptosis. However, whether or not ZnO NPs could promote autophagy, and the possible role of autophagy in the progress of apoptosis, remain unclear. In the current study, in vitro and in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20164042
更新日期:2019-08-19 00:00:00
abstract::CYP353D1v2 is a cytochrome P450 related to imidacloprid resistance in Laodelphax striatellus. This work was conducted to examine the ability of CYP353D1v2 to metabolize other insecticides. Carbon monoxide difference spectra analysis indicates that CYP353D1v2 was successfully expressed in insect cell Sf9. The catalytic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18122564
更新日期:2017-11-29 00:00:00
abstract::Members of the mitochondrial carrier (MC) protein family transport various molecules across the mitochondrial inner membrane to interlink steps of metabolic pathways and biochemical processes that take place in different compartments; i.e., are localized partly inside and outside the mitochondrial matrix. MC substrate...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20184456
更新日期:2019-09-10 00:00:00
abstract::Microglia are first-line defense antigen-presenting phagocytes in the central nervous system. Activated microglial cells release pro-inflammatory cytokines and can trigger an oxidative burst. The amino acid glycine exerts anti-inflammatory, immunomodulatory and cytoprotective effects and influences cell volume regulat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030804
更新日期:2020-01-26 00:00:00
abstract::Esophageal squamous cell carcinoma (ESCC), a major histologic type of esophageal cancer, is one of the frequent causes of cancer-related death worldwide. Picropodophyllotoxin (PPT) is the main component of Podophyllum hexandrum root with antitumor activity via apoptosis-mediated mechanisms in several cancer cells. How...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134640
更新日期:2020-06-30 00:00:00
abstract::The aim of this study was to explore the optimal therapeutic dose and time window of picroside II for treating cerebral ischemic injury in rats according to the orthogonal test. The middle cerebral artery occlusion (MCAO) models were established by intraluminally inserting a thread into middle cerebral artery (MCA) fr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13032551
更新日期:2012-01-01 00:00:00