Abstract：
:Hibernation is an effective energy conservation strategy that has been widely adopted by animals to cope with unpredictable environmental conditions. The liver, in particular, plays an important role in adaptive metabolic adjustment during hibernation. Mammalian studies have revealed that many genes involved in metabolism are differentially expressed during the hibernation period. However, the differentiation in global gene expression between active and torpid states in amphibians remains largely unknown. We analyzed gene expression in the liver of active and torpid Asiatic toads (Bufo gargarizans) using RNA-sequencing. In addition, we evaluated the differential expression of genes between females and males. A total of 1399 genes were identified as differentially expressed between active and torpid females. Of these, the expressions of 395 genes were significantly elevated in torpid females and involved genes responding to stresses, as well as contractile proteins. The expression of 1004 genes were significantly down-regulated in torpid females, most which were involved in metabolic depression and shifts in the energy utilization. Of the 715 differentially expressed genes between active and torpid males, 337 were up-regulated and 378 down-regulated. A total of 695 genes were differentially expressed between active females and males, of which 655 genes were significantly down-regulated in males. Similarly, 374 differentially expressed genes were identified between torpid females and males, with the expression of 252 genes (mostly contractile proteins) being significantly down-regulated in males. Our findings suggest that expression of many genes in the liver of B. gargarizans are down-regulated during hibernation. Furthermore, there are marked sex differences in the levels of gene expression, with females showing elevated levels of gene expression as compared to males, as well as more marked down-regulation of gene-expression in torpid males than females.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Jin L,Yu JP,Yang ZJ,Merilä J,Liao WBdoi
10.3390/ijms19082363subject
Has Abstractpub_date
2018-08-10 00:00:00issue
8issn
1422-0067pii
ijms19082363journal_volume
19pub_type
杂志文章abstract：:There is evidence that spaceflight poses acute and late risks to the central nervous system. To explore possible mechanisms, the proteomic changes following spaceflight in mouse brain were characterized. Space Shuttle Atlantis (STS-135) was launched from the Kennedy Space Center (KSC) on a 13-day mission. Within 3⁻5 h...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010007
更新日期：2018-12-20 00:00:00
abstract：:Antibiotic-loaded bone graft substitutes are attractive clinical options and have been used for years either for prophylaxis or therapy for periprosthetic and fracture-related infections. Calcium sulfate and hydroxyapatite can be combined in an injectable and moldable bone graft substitute that provides dead space man...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239250
更新日期：2020-12-04 00:00:00
abstract：:The reaction product of nitric oxide and superoxide, peroxynitrite, is a potent biological oxidant. The most important oxidative protein modifications described for peroxynitrite are cysteine-thiol oxidation and tyrosine nitration. We have previously demonstrated that intrinsic heme-thiolate (P450)-dependent enzymatic...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14047542
更新日期：2013-04-08 00:00:00
abstract：:The last step in the biosynthesis of flavin adenine dinucleotide (FAD) is considered a target for the design of antimicrobial drugs because it is carried out by two non-homologous proteins in eukaryotic and prokaryotic organisms. Monofunctional FMN: adenylyltransferases (FMNAT) in Eukarya and FMNAT modules of bifuncti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103738
更新日期：2020-05-25 00:00:00
abstract：:We show that, under in vitro conditions, the vulnerability of astroglia to hypoxia is reflected by alterations in endothelin (ET)-1 release and capacity of erythropoietin (EPO) to regulate ET-1 levels. Exposure of cells to 24 h hypoxia did not induce changes in ET-1 release, while 48-72 h hypoxia resulted in increase ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15022858
更新日期：2014-02-19 00:00:00
abstract：:Following herbivore attacks, plants modify a blend of volatiles organic compounds (VOCs) released, resulting in the attraction of their antagonists. However, volatiles released constitutively may affect herbivores and natural enemies' fitness too. In tomato there is still a lack of information on the genetic bases res...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092748
更新日期：2018-09-13 00:00:00
abstract：:Lead and lead-derived compounds have been extensively utilized in industry, and their chronic toxicity towards aquatic animals has not been thoroughly addressed at a behavioral level. In this study, we assessed the risk of exposure to lead at a waterborne environmental concentration in adult zebrafish by behavioral an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051844
更新日期：2020-03-07 00:00:00
abstract：:The 12-oxo-phytodienoic acid reductases (OPRs), which belong to the old yellow enzyme (OYE) family, are flavin mononucleotide (FMN)-dependent oxidoreductases with critical functions in plants. Despite the clear characteristics of growth and development, as well as the defense responses in Arabidopsis, tomato, rice, an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081914
更新日期：2019-04-18 00:00:00
abstract：:Zinc finger protein 554 (ZNF554), a member of the Krüppel-associated box domain zinc finger protein subfamily, is predominantly expressed in the brain and placenta in humans. Recently, we unveiled that ZNF554 regulates trophoblast invasion during placentation and its decreased expression leads to the early pathogenesi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165762
更新日期：2020-08-11 00:00:00
abstract：:The silkworm is an oligophagous insect for which mulberry leaves are the sole diet. The nutrients needed for vital activities of the egg, pupal, and adult stages, and the proteins formed in the cocoon, are all derived from the larval stages. The silkworm feeds and grows quickly during the larval stages. In particular,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246113
更新日期：2019-12-04 00:00:00
abstract：:NASH is becoming increasingly common worldwide because of the growing global prevalence of obesity and consequently NAFLD. Unfortunately, the mechanism of progression of NAFLD to NASH and then cirrhosis is not completely understood. Several factors, including insulin resistance, inflammation, oxidative stress, lipotox...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19072034
更新日期：2018-07-13 00:00:00
abstract：:Chronic kidney disease (CKD) is associated with atherogenic dyslipidemia. Our aim was firstly to investigate patterns of fatty acids (FA) composition through various stages of CKD, and secondly, to evaluate the effect of CKD-specific FA disturbances on the expression of genes related to lipid metabolism at a cellular ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102470
更新日期：2019-05-18 00:00:00
abstract：:Broiler breeder hens with efficient feed conversion rate under restricted feed intake (R-hens) or allowed unlimited access to feed (Ad-hens) progressed with cardiac functional failure and suffered early sudden death. A supplement of 69 μg 25-hydroxycholecalciferol (25-OH-D3)/kg feed improved heart health and rescued l...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218379
更新日期：2020-11-08 00:00:00
abstract：:Heterosis has been widely accepted as an effective strategy to increase yields in plant breeding. Notably, the chemical hybridization agent SQ-1 induces male sterility in wheat, representing a critical potential tool in hybrid seed production. However, the mechanisms underlying the male sterility induced by SQ-1 still...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072468
更新日期：2020-04-02 00:00:00
abstract：:Akt acts as a pivotal regulator in the PI3K/Akt signaling pathway and represents a potential drug target for cancer therapy. To search for new inhibitors of Akt kinase, we performed a structure-based virtual screening using the DOCK 4.0 program and the X-ray crystal structure of human Akt kinase. From the virtual scre...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16023202
更新日期：2015-02-02 00:00:00
abstract：:Rett syndrome (RTT) is an early-onset neurodevelopmental disorder that primarily affects females, resulting in severe cognitive and physical disabilities, and is one of the most prevalent causes of intellectual disability in females. More than fifty years after the first publication on Rett syndrome, and almost two de...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20163925
更新日期：2019-08-12 00:00:00
abstract：:Cannabinoids, the biologically active constituents of Cannabis, have potent neuronal and immunological effects. However, the basic and medical research dedicated to medical cannabis and cannabinoids is limited. The influence of these treatments on hematologic reconstitution and on the development of graft versus host ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030668
更新日期：2019-02-04 00:00:00
abstract：:Ischemic stroke is a leading cause of human death in present times. Two phases of pathological impact occur during an ischemic stroke, namely, ischemia and reperfusion. Both periods include individual characteristic effects on cell injury and apoptosis. Moreover, these conditions can cause severe cell defects and harm...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246168
更新日期：2019-12-06 00:00:00
abstract：:The ongoing pandemic of coronavirus disease-2019 (COVID-19) is being caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The disease continues to present significant challenges to the health care systems around the world. This is primarily because of the lack of vaccines to protect against the infe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155224
更新日期：2020-07-23 00:00:00
abstract：:The development of atherosclerosis is a multi-step process, at least in part controlled by the vascular endothelium function. Observations in humans and experimental models of atherosclerosis have identified monocyte recruitment as an early event in atherogenesis. Chronic inflammation is associated with ageing and its...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms140612696
更新日期：2013-06-17 00:00:00
abstract：:Melatonin is an indoleamine produced from the amino acid l-tryptophan, whereas metabolites of melatonin are known as kynuramines. One of the best-known kynuramines is N¹-acetyl-N¹-formyl-5-methoxykynuramine (AFMK). Melatonin has attracted scientific attention as a potent antioxidant and protector of tissue against oxi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18051014
更新日期：2017-05-08 00:00:00
abstract：:Extracellular vesicles (EVs) are membrane-bound particles involved in intercellular communication. They carry proteins, lipids, and nucleotides such as microRNAs (miRNAs) from the secreting cell that can modulate target cells. We and others have previously described the presence of EVs in peripheral blood of multiple ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082448
更新日期：2018-08-19 00:00:00
abstract：:Spontaneous beating of the heart pacemaker, the sinoatrial node, is generated by sinoatrial node cells (SANC) and caused by gradual change of the membrane potential called diastolic depolarization (DD). Submembrane local Ca2+ releases (LCR) from sarcoplasmic reticulum (SR) occur during late DD and activate an inward N...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19082173
更新日期：2018-07-25 00:00:00
abstract：:The reliability of reverse transcription-quantitative PCR (RT-qPCR) results in gene expression studies depends on the approaches used to account for non-biological variations. In order to find a proper normalization strategy for the study of genes related to growth hormone signaling in skeletal muscle of growing mice,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051060
更新日期：2017-05-16 00:00:00
abstract：:Fourier transform infrared spectroscopy (FTIR) can provide crucial information on the molecular structure of organic and inorganic components and has been used extensively for chemical characterization of geological samples in the past few decades. In this paper, recent applications of FTIR in the geological sciences ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161226227
更新日期：2015-12-18 00:00:00
abstract：:The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15034583
更新日期：2014-03-17 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) is often diagnosed at a late stage and may be malignantly transformed from oral leukoplakia (OL). This study aimed to identify potential plasma microRNAs (miRNAs) for the early detection of oral cancer. Plasma from normal, OL, and OSCC patients were evaluated. Small RNA sequencing w...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030758
更新日期：2018-03-07 00:00:00
abstract：:Liquid biopsy-based methods to test biomarkers (e.g., serum proteins and extracellular vesicles) may help to monitor brain tumors. In this proteomics-based study, we aimed to identify a characteristic protein fingerprint associated with central nervous system (CNS) tumors. Overall, 96 human serum samples were obtained...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155359
更新日期：2020-07-28 00:00:00
abstract：:Synthesis and anticonvulsant potential of certain new 6-aryl-9-substituted-6,9-diazaspiro[4.5]decane-8,10-diones (6a-l) and 1-aryl-4-substituted-1,4-diazaspiro[5.5] undecane-3,5-diones (6m-x) are reported. The intermediates 1-[(aryl)(cyanomethyl)amino] cycloalkanecarboxamides (3a-f) were prepared via adopting Strecker...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150916911
更新日期：2014-09-23 00:00:00
abstract：:Fetuin-A is the protein product of the AHSG gene in humans. It is mainly synthesized by the liver in adult humans and is secreted into the blood where its concentration can vary from a low of ~0.2 mg/mL to a high of ~0.8 mg/mL. Presently, it is considered to be a multifunctional protein that plays important roles in d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19082211
更新日期：2018-07-29 00:00:00
