Abstract:
:Metabolic network rewiring is the rerouting of metabolism through the use of alternate enzymes to adjust pathway flux and accomplish specific anabolic or catabolic objectives. Here, we report the first characterization of two parallel pathways for the breakdown of the short chain fatty acid propionate in Caenorhabditis elegans. Using genetic interaction mapping, gene co-expression analysis, pathway intermediate quantification and carbon tracing, we uncover a vitamin B12-independent propionate breakdown shunt that is transcriptionally activated on vitamin B12 deficient diets, or under genetic conditions mimicking the human diseases propionic- and methylmalonic acidemia, in which the canonical B12-dependent propionate breakdown pathway is blocked. Our study presents the first example of transcriptional vitamin-directed metabolic network rewiring to promote survival under vitamin deficiency. The ability to reroute propionate breakdown according to B12 availability may provide C. elegans with metabolic plasticity and thus a selective advantage on different diets in the wild.
journal_name
Elifejournal_title
eLifeauthors
Watson E,Olin-Sandoval V,Hoy MJ,Li CH,Louisse T,Yao V,Mori A,Holdorf AD,Troyanskaya OG,Ralser M,Walhout AJdoi
10.7554/eLife.17670subject
Has Abstractpub_date
2016-07-06 00:00:00issn
2050-084Xjournal_volume
5pub_type
杂志文章相关文献
eLife文献大全abstract::The time it takes a sound to travel from source to ear differs between the ears and creates an interaural delay. It varies systematically with spatial direction and is generally modeled as a pure time delay, independent of frequency. In acoustical recordings, we found that interaural delay varies with frequency at a f...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06072
更新日期:2015-04-27 00:00:00
abstract::Most evolutionary thinking is based on the notion of fitness and related ideas such as fitness landscapes and evolutionary optima. Nevertheless, it is often unclear what fitness actually is, and its meaning often depends on the context. Here we argue that fitness should not be a basal ingredient in verbal or mathemati...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23804
更新日期:2017-02-15 00:00:00
abstract::Although it is clear that trisomy 21 causes Down syndrome, the molecular events acting downstream of the trisomy remain ill defined. Using complementary genomics analyses, we identified the interferon pathway as the major signaling cascade consistently activated by trisomy 21 in human cells. Transcriptome analysis rev...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16220
更新日期:2016-07-29 00:00:00
abstract::How circuits assemble starting from stem cells is a fundamental question in developmental neurobiology. We test the hypothesis that, in neuronal stem cells, temporal transcription factors predictably control neuronal terminal features and circuit assembly. Using the Drosophila motor system, we manipulate expression of...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46089
更新日期:2019-09-10 00:00:00
abstract::The emergence of subpopulations that perform distinct metabolic roles has been observed in populations of genetically identical bacteria. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.38578
更新日期:2018-06-29 00:00:00
abstract::Squamous cell carcinomas occurring at transition zones are highly malignant tumors with poor prognosis. The identity of the cell population and the signaling pathways involved in the progression of transition zone squamous cell carcinoma are poorly understood, hence representing limited options for targeted therapies....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22914
更新日期:2017-02-21 00:00:00
abstract::Decisions are accompanied by a degree of confidence that a selected option is correct. A sequential sampling framework explains the speed and accuracy of decisions and extends naturally to the confidence that the decision rendered is likely to be correct. However, discrepancies between confidence and accuracy suggest ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12192
更新日期:2016-02-01 00:00:00
abstract::Regulation of food intake is fundamental to energy homeostasis in animals. The contribution of non-nutritive and metabolic signals in regulating feeding is unclear. Here we show that enteric neurons play a major role in regulating feeding through specialized mechanosensory ion channels in Drosophila. Modulating activi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04402
更新日期:2014-10-06 00:00:00
abstract::Periodontal disease is an age-associated disorder clinically defined by periodontal bone loss, inflammation of the specialized tissues that surround and support the tooth, and microbiome dysbiosis. Currently, there is no therapy for reversing periodontal disease, and treatment is generally restricted to preventive mea...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54318
更新日期:2020-04-28 00:00:00
abstract::The basolateral amygdala (BLA) plays a vital role in associating sensory stimuli with salient valence information. Excitatory principal neurons (PNs) undergo plastic changes to encode this association; however, local BLA inhibitory interneurons (INs) gate PN plasticity via feedforward inhibition (FFI). Despite literat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50601
更新日期:2020-01-09 00:00:00
abstract::Increasing evidence highlights the important roles of microRNAs in mediating p53's tumor suppression functions. Here, we report miR-139-5p as another new p53 microRNA target. p53 induced the transcription of miR-139-5p, which in turn suppressed the protein levels of phosphodiesterase 4D (PDE4D), an oncogenic protein i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15978
更新日期:2016-07-07 00:00:00
abstract::RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid RNA/protein phase. Which model describes RNP granules in living cells is still unclear. In this study, we analyze P bodies in budding yeast and find that they have liquid-like pro...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06807
更新日期:2015-08-04 00:00:00
abstract::Feedback inhibitory motifs are thought to be important for pattern separation across species. How feedback circuits may implement pattern separation of biologically plausible, temporally structured input in mammals is, however, poorly understood. We have quantitatively determined key properties of netfeedback inhibiti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53148
更新日期:2020-02-20 00:00:00
abstract::Drosophila melanogaster males perform a series of courtship behaviors that, when successful, result in copulation with a female. For over a century, mutations in the yellow gene, named for its effects on pigmentation, have been known to reduce male mating success. Prior work has suggested that yellow influences mating...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49388
更新日期:2019-10-15 00:00:00
abstract::bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Fu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17537
更新日期:2016-10-28 00:00:00
abstract::Over 80% of multiple-tested siRNAs and shRNAs targeting CD95 or CD95 ligand (CD95L) induce a form of cell death characterized by simultaneous activation of multiple cell death pathways preferentially killing transformed and cancer stem cells. We now show these si/shRNAs kill cancer cells through canonical RNAi by targ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29702
更新日期:2017-10-24 00:00:00
abstract::Using a common analysis pipeline to compare data from three major lineages of complex eukaryotes reveals that transcription seems to evolve at a common rate. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.14150
更新日期:2016-02-11 00:00:00
abstract::During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome. We show here that deep sequencing of ribosome-protected mRNA fragments reveals ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01257
更新日期:2014-05-09 00:00:00
abstract::Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remai...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.35621
更新日期:2018-06-25 00:00:00
abstract::Secondary metabolites have a great potential as pharmaceuticals, but there are only a few examples where regulation of gene cluster expression has been correlated with ecological and physiological relevance for the producer. Here, signals, mediators, and biological effects of terrein production were studied in the fun...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07861
更新日期:2015-07-14 00:00:00
abstract::As part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Blum et al., 2015), that described how we intended to replicate selected experiments from the paper 'Transcriptional amplification in tumor cells with elevated c-Myc' (Lin et al., 2012). Here we report the results. We found overe...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30274
更新日期:2018-01-09 00:00:00
abstract::White adipose tissue (WAT) inflammation contributes to the development of insulin resistance in obesity. While the role of adipose tissue macrophage (ATM) pro-inflammatory signalling in the development of insulin resistance has been established, it is less clear how WAT inflammation is initiated. Here, we show that AT...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47990
更新日期:2019-08-16 00:00:00
abstract::Neurons coding for head-direction are crucial for spatial navigation. Here we explored the cellular basis of head-direction coding in the rat dorsal presubiculum (PreS). We found that layer2 is composed of two principal cell populations (calbindin-positive and calbindin-negative neurons) which targeted the contralater...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14592
更新日期:2016-06-10 00:00:00
abstract::Dengue Virus (DENV), a flavivirus spread by mosquito vectors, can cause vascular leakage and hemorrhaging. However, the processes that underlie increased vascular permeability and pathological plasma leakage during viral hemorrhagic fevers are largely unknown. Mast cells (MCs) are activated in vivo during DENV infecti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00481
更新日期:2013-04-30 00:00:00
abstract::Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concent...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37960
更新日期:2018-10-11 00:00:00
abstract::The choroid, which provides vascular supply to the outer retina, demonstrates progressive degeneration in aging and age-related macular degeneration (AMD). However mechanisms that maintain or compromise choroidal homeostasis are obscure. We discovered that the ablation of choroidal macrophages via CSF1R blockade was a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55564
更新日期:2020-04-01 00:00:00
abstract::We present a reanalysis of the stochastic model of organelle production and show that the equilibrium distributions for the organelle numbers predicted by this model can be readily calculated in three different scenarios. These three distributions can be identified as standard distributions, and the corresponding exac...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10167
更新日期:2015-12-22 00:00:00
abstract::The discovery of genomic imprinting by Davor Solter, Azim Surani and co-workers in the mid-1980s has provided a foundation for the study of epigenetic inheritance and the epigenetic control of gene activity and repression, especially during development. It also has shed light on a range of diseases, including both rar...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42368
更新日期:2018-10-22 00:00:00
abstract::Holliday junctions (HJs) are key DNA intermediates in homologous recombination. They link homologous DNA strands and have to be faithfully removed for proper DNA segregation and genome integrity. Here, we present the crystal structure of human HJ resolvase GEN1 complexed with DNA at 3.0 Å resolution. The GEN1 core is ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12256
更新日期:2015-12-18 00:00:00
abstract::Newly born cells either continue to proliferate or exit the cell division cycle. This decision involves delaying expression of Cyclin E that promotes DNA replication. ORC1, the Origin Recognition Complex (ORC) large subunit, is inherited into newly born cells after it binds to condensing chromosomes during the precedi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12785
更新日期:2016-07-26 00:00:00