Abstract：
UNLABELLED:Sepsis is the major cause of morbidity and mortality in children, especially in immunocompromised patients, and a rapid identification of causative pathogen is strongly related with a better outcome. This prospective study analyzes the role of a multiplex real-time polymerase chain reaction in sepsis' etiological diagnosis. Magicplex(TM) Sepsis Real-Time tests were performed in tertiary Regina Margherita Children's Hospital (Turin, Italy), and the medical records of children who underwent a Magicplex test were prospectively evaluated. Results of the Magicplex test were compared with those of blood culture collected at a close time point. One hundred fifty Magicplex tests were collected from 89 patients (54 males and 35 females, age interquartile range: 2.6-12.1 years). Etiological definition was achieved in 60 bloodstream infection cases (40 %). In 32 episodes, Magicplex test alone gave a positive result, and blood culture alone permitted the etiological diagnosis in 5 septic episodes. Magicplex test allowed a 143 % increase in the diagnostic value of blood cultures. CONCLUSION:These results suggest that molecular biology can be useful for rapid pathogen's identification also in children. WHAT IS KNOWN:• Sepsis represents a major cause of morbidity and mortality in children. • Sepsis outcome is strongly related to rapid microbiological identification and prompt initiation of an appropriate chemotherapy. What is New: • This manuscript is the first that describes the use of Magicplex (TM) Sepsis Real-Time test in children. • The results suggest that molecular biology can be useful for rapid pathogen's identification also in children.
journal_name
Eur J Pediatrjournal_title
European journal of pediatricsauthors
Denina M,Scolfaro C,Colombo S,Calitri C,Garazzino S,Barbui Anna A,Brossa S,Regina Margherita Children’s Hospital Bloodstream Infections Study Group participants.,Tovo PAdoi
10.1007/s00431-016-2745-3subject
Has Abstractpub_date
2016-08-01 00:00:00pages
1107-11issue
8eissn
0340-6199issn
1432-1076pii
10.1007/s00431-016-2745-3journal_volume
175pub_type
杂志文章abstract：INTRODUCTION:Impaired vitamin K status in cystic fibrosis (CF) has been considered as a newly emerged pathogenetic factor for reduced bone mineral density (BMD). OBJECTIVES:Our aim was to evaluate the effectiveness of vitamin K supplementation in managing bone formation abnormalities in children and adolescents with C...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-006-0132-1
更新日期：2006-08-01 00:00:00
abstract：:Clinical and EEG family data of 140 cases with early childhood epilepsy with absences are presented. The aim of the study was to evaluate, whether the occurrence of generalized tonic clonic seizures (GTCS) as a presenting symptom might correlate with family data, i.e. whether there are indications of heterogeneity. On...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF01956423
更新日期：1994-05-01 00:00:00
abstract：:Ethnic classification does not correlate well with skin tone. As there are no neonatal skin color scales, we aimed to create and validate one of our own. After creating the scale and briefly training our staff, we conducted a prospective, observational study to assess reproducibility and correlation of each scale colo...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-020-03623-6
更新日期：2020-09-01 00:00:00
abstract：:Renal tubular dysgenesis is a critical disorder characterized by the Potter phenotype and severe hypotension in the early neonatal period. We herein report a 3-year-old female with renal tubular dysgenesis. Endocrinological studies showed a high plasma renin activity (over 49.2 ng/ml/h; normal range 2.0-15.2), high ac...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-008-0743-9
更新日期：2009-02-01 00:00:00
abstract：:We report the case of 5-week-old male infant who presented as a 'near miss cot death'. He had the immunodeficient syndrome of defective neutrophil mobility and delayed umbilical cord separation. He was shown to have staphylococcal endocarditis with a large vegetation on the mitral valve, and acute obstruction of the m...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF02429063
更新日期：1986-12-01 00:00:00
abstract：:A five-year-old-girl with a history of recurrent hypoglycemia presented with acidosis, intractable vomiting, and abdominal tenderness; the diagnosis of acute pancreatitis was made by abdominal ultrasonography and supportive biochemical studies. Urinary organic acid analysis revealed metabolites suggestive of HMG-CoA l...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF00540255
更新日期：1984-09-01 00:00:00
abstract：:A new method for the detection of hyperammonemia, using a kit based on the principle of microdiffusion of ammonia, is described. The method requires only one drop of blood and takes only 15 min to complete. Experiments for recovery and reproducibility were satisfactory, and good correlation was obtained when compared ...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF00442347
更新日期：1979-02-08 00:00:00
abstract：:Our prior studies of the molecular epidemiology of group A streptococcus (GAS) pharyngitis indicated that the most common emm types associated with pediatric pharyngitis in North America were 12, 1, 28, and 4. We previously reported that the proportions of pediatric pharyngitis due to emm types 12 and 4 decreased with...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-008-0819-6
更新日期：2009-06-01 00:00:00
abstract：:The Kabuki (Niikawa-Kuroki) syndrome was reported in 1981 by Niikawa et al. and Kuroki et al. in a total of ten unrelated Japanese children with a characteristic array of multiple congenital anomalies and mental retardation. The syndrome is characterized by a distinct face, mild to moderate mental retardation, postnat...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF01983409
更新日期：1994-06-01 00:00:00
abstract：UNLABELLED:A total of 22 Japanese patients with hypophosphatasia were included in a study analysing the relationship between mutations in the tissue-nonspecific alkaline phosphatase (TNSALP) gene and the severity of the phenotype in Japanese patients with hypophosphatasia. The enzymatic activity of some of the identifi...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-004-1612-9
更新日期：2005-05-01 00:00:00
abstract：:Severe hand, foot, and mouth disease (HFMD) is likely to develop critical complications such as brainstem encephalitis, acute pulmonary edema, and circulatory failure, which cause child mortality during outbreaks. This study aims to investigate factors that predict the severity of HFMD. One hundred sixteen in-patient ...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-013-1939-1
更新日期：2013-05-01 00:00:00
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journal_title：European journal of pediatrics
pub_type： 临床试验,杂志文章
doi：10.1007/s00431-013-2073-9
更新日期：2013-11-01 00:00:00
abstract：:Surfactant-associated protein (SP-A) was measured in tracheal aspirates of ventilated infants with (n = 51) and without (n = 21) respiratory distress syndrome (RDS). SP-A concentrations in samples collected after birth were significantly lower in RDS than in infants ventilated for other reasons than RDS (median 0.03 v...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF01957730
更新日期：1992-08-01 00:00:00
abstract：:The concentration of mRNA of insulin-like growth factor-II is (IGF-II) much elevated in some embryonic tumours such as Wilms tumour (nephroblastoma). In order to prove whether or not IGF-II is produced by the tumour tissue, IGF-II was extracted from freshly frozen tissue of Wilms tumour and hepatoblastoma. Normal adja...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF02072483
更新日期：1993-02-01 00:00:00
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journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF01959396
更新日期：1990-04-01 00:00:00
abstract：UNLABELLED:Complementary/alternative medicine (CAM) has become an important topic, not least because of its popularity with patients. This systematic review is aimed at summarising the data known about CAM use in paediatric populations. A thorough search strategy was designed to identify all surveys on the prevalence o...
journal_title：European journal of pediatrics
pub_type： 杂志文章,评审
doi：10.1007/s004310051000
更新日期：1999-01-01 00:00:00
abstract：:Metabolic liver diseases (MLD) are an important group of disorders presenting with neonatal cholestasis (NC). The spectrum of liver involvement is wide and the presumptive diagnosis is traditionally based on clinical and laboratory findings. Recently, next-generation sequencing (NGS) panels have emerged as an appealin...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-019-03328-5
更新日期：2019-04-01 00:00:00
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journal_title：European journal of pediatrics
pub_type： 杂志文章
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abstract：:Chronic granulomatous disease (CGD) is a rare primary immunodeficiency caused by mutations of one of the subunits of phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase leading to decreased or complete absence of neutrophil oxidative burst. We report the clinical and laboratory findings in tw...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-006-0211-3
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journal_title：European journal of pediatrics
pub_type： 杂志文章,评审
doi：10.1007/BF01972883
更新日期：1994-11-01 00:00:00
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journal_title：European journal of pediatrics
pub_type： 杂志文章,评审
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更新日期：1998-05-01 00:00:00
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journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF00441860
更新日期：1986-04-01 00:00:00
abstract：:A 22 years old female with the late-onset of argininosuccinic aciduria was successfully treated for 5 months with a mixture of essential amino acids and their keto-analogues. There was a marked change in plasma ammonia, plasma amino acids and argininosuccinic acid excretion. A long term anabolic response was reflected...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF00445607
更新日期：1978-07-19 00:00:00
abstract：UNLABELLED:It has been suggested that chronic treatment with L-thyroxine (L-T4) could be implicated in reducing bone mineral density (BMD). The purpose of this longitudinal study was to determine whether appendicular and axial BMD is decreased by L-T4 treatment in adolescent girls. Thirteen adolescent girls with subcli...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF01955180
更新日期：1996-06-01 00:00:00
abstract：:Diagnostic tools for the management of acute osteomyelitis (OM) and septic arthritis (SA) have improved over the last decade. To investigate the influence and availability of magnetic resonance imaging (MRI) and nucleic acid testing (NAT), a retrospective cohort study was done. Patients admitted with acute OM or SA be...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/s00431-018-3157-3
更新日期：2018-07-01 00:00:00
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journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF00451907
更新日期：1985-07-01 00:00:00
abstract：:We report what we believe to be the first case of simultaneous infection by Toxoplasma gondii and cytomegalovirus in a newborn. ...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF00496426
更新日期：1988-05-01 00:00:00
abstract：:A case of painful bilateral hip effusions resolving spontaneously in the diuretic phase of steroid responsive nephrotic syndrome in a 6-year-old girl is reported. ...
journal_title：European journal of pediatrics
pub_type： 杂志文章
doi：10.1007/BF02072499
更新日期：1993-02-01 00:00:00
