Coxiella burnetii effector CvpB modulates phosphoinositide metabolism for optimal vacuole development.

abstract：:Recombinant immunotoxins (RITs) are chimeric proteins composed of an Fv and a protein toxin being developed for cancer treatment. The Fv brings the toxin to the cancer cell, but most of the RITs do not reach the tumor and are removed by other organs. To identify cells responsible for RIT removal, and the pathway by wh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu XF,Wei J,Zhou Q,Molitoris BA,Sandoval R,Kobayashi H,Okada R,Nagaya T,Karim B,Butcher D,Pastan I

更新日期：2020-03-17 00:00:00

abstract：:Bacteriophage lambda encodes a number of genes involved in the recombinational repair of DNA double-strand breaks. The product of one of these genes, rap, has been purified. Truncated Rap proteins that copurify with the full-length form are derived, at least in part, from a rho-dependent transcription terminator locat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharples GJ,Corbett LM,Graham IR

更新日期：1998-11-10 00:00:00

abstract：:When the human cDNA, isolated on the basis of homology to the murine carbonic anhydrase (CA) "Y" was expressed in COS cells, the human CA was targeted to and processed in mitochondria, as expected for CA-V. However, tissue distribution reported for the corresponding mouse CA Y mRNA was much more limited than that repo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagao Y,Srinivasan M,Platero JS,Svendrowski M,Waheed A,Sly WS

更新日期：1994-10-25 00:00:00

abstract：:Unintended anthropogenic deposition of sulfur (S) to forest ecosystems has a range of negative consequences, identified through decades of research. There has been far less study of purposeful S use in agricultural systems around the world, including the application of elemental sulfur (S(0)) as a quick-reacting fungi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hinckley EL,Matson PA

更新日期：2011-08-23 00:00:00

abstract：:Tobacco mosaic virus (TMV) RNA was encapsulated in large, unilamellar phospholipid vesicles (liposomes), and the encapsulated TMV RNA was shown to be infectious when incubated with tobacco protoplasts under appropriate conditions. Maximal virus production in protoplasts was observed after their incubation with TMV RNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fraley RT,Dellaporta SL,Papahadjopoulos D

更新日期：1982-03-01 00:00:00

abstract：:The fractionation of cells of a parsley suspension culture [Petroselinum crispum (Mill.) A. Hill] by protoplasting and subsequent removal of the vacuoles led to physiologically intact evacuolated protoplasts retaining light inducibility of chalcone synthase expression. Lysis of the evacuolated protoplasts permitted th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harter K,Frohnmeyer H,Kircher S,Kunkel T,Mühlbauer S,Schäfer E

更新日期：1994-05-24 00:00:00

abstract：:Two deletion-substitution mutations of phage lambda, spi-113 and spi-274, are shown to remove about half of the rightward operator OR. Physiological studies show that spi-113 is still repressible. Thus, the 50 or so nucleotides of OR deleted in this mutant are not absolutely essential for repression. prm-116, which in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith GR,Eisen H,Reichardt L,Hedgepeth J

更新日期：1976-03-01 00:00:00

abstract：:The 5-hydroxytryptamine-3 (5-HT3) receptor mediates the fast excitatory neurotransmission of serotonin and is known to mediate the nausea/emesis induced by radio/chemotherapy and anesthetics. A polymorphism encoding the variation Y129S in the 5-HT3B subunit exists in high frequency in the general population and has be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krzywkowski K,Davies PA,Feinberg-Zadek PL,Bräuner-Osborne H,Jensen AA

更新日期：2008-01-15 00:00:00

abstract：:Cytosine methylation at CpG dinucleotides contributes to the epigenetic maintenance of gene silencing. Dynamic reprogramming of DNA methylation patterns is believed to play a key role during development and differentiation in vertebrates. The mechanisms of DNA demethylation remain unclear and controversial. Here, we p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kress C,Thomassin H,Grange T

更新日期：2006-07-25 00:00:00

abstract：:Confluent cultures of human fetal lung fibroblasts degrade approximately 10% of their newly synthesized collagen within the cell prior to secretion. This basal level of intracellular degradation could not be inhibited by colchicine or cytochalasin B, inhibitors of microtubular and microfilament function, respectively,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berg RA,Schwartz ML,Crystal RG

更新日期：1980-08-01 00:00:00

abstract：:Helicobacter pylori VacA, a pore-forming toxin secreted by an autotransporter pathway, causes multiple alterations in human cells, contributes to the pathogenesis of peptic ulcer disease and gastric cancer, and is a candidate antigen for inclusion in an H. pylori vaccine. Here, we present a 2.4-A crystal structure of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gangwer KA,Mushrush DJ,Stauff DL,Spiller B,McClain MS,Cover TL,Lacy DB

更新日期：2007-10-09 00:00:00

abstract：:When various wild strains of Penicillium lanosum and Aspergillus niger were placed in the same mild laboratory environment, their frequencies of new spontaneous mutations were clearly related to whether they had been isolated from a region of high or low microclimatic stress. In the mild environment, the total frequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lamb BC,Mandaokar S,Bahsoun B,Grishkan I,Nevo E

更新日期：2008-04-15 00:00:00

abstract：:There is increasing evidence for an important role of adverse early experience on the development of major psychiatric disorders in adulthood. Corticotropin-releasing factor (CRF), an endogenous neuropeptide, is the primary physiological regulator of the mammalian stress response. Grown nonhuman primates who were expo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coplan JD,Andrews MW,Rosenblum LA,Owens MJ,Friedman S,Gorman JM,Nemeroff CB

更新日期：1996-02-20 00:00:00

abstract：:Assembly of nitrogenase FeMoco is one of the key processes in bioinorganic chemistry. NifB and NifEN are two essential elements immediately adjacent to each other along the biosynthetic pathway of FeMoco. Previously, an 8Fe-precursor of FeMoco was identified on NifEN; however, the identity of the biosynthetic intermed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiig JA,Hu Y,Ribbe MW

更新日期：2011-05-24 00:00:00

abstract：:TAR DNA-binding protein-43 (TDP-43), a DNA/RNA-binding protein involved in RNA transcription and splicing, has been associated with the pathophysiology of neurodegenerative diseases, including ALS. However, the function of TDP-43 in motor neurons remains undefined. Here we use both gain- and loss-of-function approache...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shan X,Chiang PM,Price DL,Wong PC

更新日期：2010-09-14 00:00:00

abstract：:Ubiquitin C-terminal hydrolase L1 (UCH-L1) is one of the most abundant and enigmatic enzymes of the CNS. Based on existing UCH-L1 knockout models, UCH-L1 is thought to be required for the maintenance of axonal integrity, but not for neuronal development despite its high expression in neurons. Several lines of evidence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reinicke AT,Laban K,Sachs M,Kraus V,Walden M,Damme M,Sachs W,Reichelt J,Schweizer M,Janiesch PC,Duncan KE,Saftig P,Rinschen MM,Morellini F,Meyer-Schwesinger C

更新日期：2019-04-16 00:00:00

abstract：:We know a great deal about the cellular response to starvation via AMPK, but less is known about the reaction to nutrient excess. Insulin resistance may be an appropriate response to nutrient excess, but the cellular sensors that link these parameters remain poorly defined. In the present study we provide evidence tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoehn KL,Salmon AB,Hohnen-Behrens C,Turner N,Hoy AJ,Maghzal GJ,Stocker R,Van Remmen H,Kraegen EW,Cooney GJ,Richardson AR,James DE

更新日期：2009-10-20 00:00:00

abstract：:Antimicrobial peptides constitute an important component of the mammalian innate immune response. Several types of antimicrobial peptides, including the beta-defensins, are produced at epithelial surfaces in response to infectious threats. Here we show that a class of small molecules, including l-isoleucine and severa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fehlbaum P,Rao M,Zasloff M,Anderson GM

更新日期：2000-11-07 00:00:00

abstract：:By using harmonic radar, we report the complete flight paths of displaced bees. Test bees forage at a feeder or are recruited by a waggle dance indicating the feeder. The flights are recorded after the bees are captured when leaving the hive or the feeder and are released at an unexpected release site. A sequence of b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Menzel R,Greggers U,Smith A,Berger S,Brandt R,Brunke S,Bundrock G,Hülse S,Plümpe T,Schaupp F,Schüttler E,Stach S,Stindt J,Stollhoff N,Watzl S

更新日期：2005-02-22 00:00:00

abstract：:In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decame...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maita N,Okada K,Hatakeyama K,Hakoshima T

更新日期：2002-02-05 00:00:00

abstract：:In addition to their roles in desensitization and signaling of seven-membrane-spanning receptors, beta-arrestins have been more recently implicated in regulating non-seven-membrane-spanning receptor pathways. By using a yeast two-hybrid screen, we identified the inhibitor of NF-kappaB, IkappaBalpha, as a binding partn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Witherow DS,Garrison TR,Miller WE,Lefkowitz RJ

更新日期：2004-06-08 00:00:00

abstract：:To better understand the roles of Sm proteins in forming the cores of many RNA-processing ribonucleoproteins, we determined the crystal structure of an atypical Sm-like archaeal protein (SmAP3) in which the conserved Sm domain is augmented by a previously uncharacterized, mixed alpha/beta C-terminal domain. The struct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mura C,Phillips M,Kozhukhovsky A,Eisenberg D

更新日期：2003-04-15 00:00:00

abstract：:Mechanotransduction in the mammalian auditory system depends on mechanosensitive channels in the hair bundles that project from the apical surface of the sensory hair cells. Individual stereocilia within each bundle contain a core of tightly packed actin filaments, whose length is dynamically regulated during developm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furness DN,Johnson SL,Manor U,Rüttiger L,Tocchetti A,Offenhauser N,Olt J,Goodyear RJ,Vijayakumar S,Dai Y,Hackney CM,Franz C,Di Fiore PP,Masetto S,Jones SM,Knipper M,Holley MC,Richardson GP,Kachar B,Marcotti W

更新日期：2013-08-20 00:00:00

abstract：:The mechanisms underlying analgesia related to social touch are not clear. While recent research highlights the role of the empathy of the observer to pain relief in the target, the contribution of social interaction to analgesia is unknown. The current study examines brain-to-brain coupling during pain with interpers...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldstein P,Weissman-Fogel I,Dumas G,Shamay-Tsoory SG

更新日期：2018-03-13 00:00:00

abstract：:Conflicts of interest between the community and its members are at the core of human social dilemmas. If observed selfishness has future costs, individuals may hide selfish acts but display altruistic ones, and peers aim at identifying the most selfish persons to avoid them as future social partners. An interaction in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rockenbach B,Milinski M

更新日期：2011-11-08 00:00:00

abstract：:To test for direct and indirect effects of a top predator on three lower trophic levels, we conducted two multiyear predator addition experiments in a tropical wet forest. Periodic additions of a top predator (predatory clerid beetle) to a wet forest understory shrub caused a reduction in the predatory beetle's prey (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dyer LA,Letourneau DK

更新日期：1999-04-27 00:00:00

abstract：:Polyploidy, the presence of more than two complete sets of chromosomes in an organism, has significantly shaped the genomes of angiosperms during evolution. Two forms of polyploidy are often considered: allopolyploidy, which originates from interspecies hybrids, and autopolyploidy, which originates from intraspecies g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Z,Haberer G,Matthes M,Rattei T,Mayer KF,Gierl A,Torres-Ruiz RA

更新日期：2010-10-12 00:00:00

abstract：:Pyochelin, an endogenous growth promoter that solubilizes ferric iron, has been isolated from Pseudomonas aeruginosa, including clinical strains. The structure of pyochelin has been assigned as 2-(2-o-hydroxyphenyl-2-thiazolin-4-yl)-3-methylthiazolidine-4-carboxylic acid and is of a different type from those previousl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cox CD,Rinehart KL Jr,Moore ML,Cook JC Jr

更新日期：1981-07-01 00:00:00

abstract：:We report direct evidence for the existence of an iron(IV)-hydroxide. Resonance Raman measurements on chloroperoxidase compound II (CPO-II) reveal an isotope ((18)O and (2)H)-sensitive band at nu(Fe-O) = 565 cm(-1). Preparation of CPO-II in H(2)O using H(2)(18)O(2) results in a red-shift of 22 cm(-1), while preparatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone KL,Behan RK,Green MT

更新日期：2006-08-15 00:00:00

abstract：:Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Velander WH,Johnson JL,Page RL,Russell CG,Subramanian A,Wilkins TD,Gwazdauskas FC,Pittius C,Drohan WN

更新日期：1992-12-15 00:00:00