A fluorescence-quenching assay for measuring permeability of reconstituted lens MIP26.

abstract：:The hydration of actin was studied by differential scanning calorimetry between -30 degrees C and 30 degrees C and by thermogravimetric analysis. The differential scanning calorimetry provided the freezable water content of G- and F-actin as a function of concentration, and the thermogravimetric analysis measured the ...

journal_title：Investigative ophthalmology & visual science

authors： Kakar S,Bettelheim FA

更新日期：1991-03-01 00:00:00

abstract：PURPOSE:To develop an imaging technique that maps the distribution of free cystine in the rat lens at subcellular resolution and enables cystine accumulation to be compared with the expression of the cystine-glutamate exchanger (Xc-). METHODS:Whole lenses were fixed, cryoprotected, and then cryosectioned in either an ...

journal_title：Investigative ophthalmology & visual science

authors： Li L,Lim J,Jacobs MD,Kistler J,Donaldson PJ

更新日期：2007-03-01 00:00:00

abstract：PURPOSE:This study aims to determine the in vivo effectiveness of low-frequency ultrasound in mediating the transport of macromolecules to the posterior segment of the eye via transscleral route. It investigates if damage is caused by ultrasound at the tested operation parameters on the posterior ocular tissues and vis...

journal_title：Investigative ophthalmology & visual science

authors： Suen WL,Wong HS,Yu Y,Lau LC,Lo AC,Chau Y

更新日期：2013-06-26 00:00:00

abstract：PURPOSE:To examine the influence of experimentally reduced cerebrospinal fluid pressure (CSFP) on retinal nerve fiber layer (RNFL) thickness and neuroretinal rim area of the optic nerve head. METHODS:This experimental study included nine monkeys that underwent implantation of a lumbar-peritoneal cerebrospinal fluid (C...

journal_title：Investigative ophthalmology & visual science

authors： Yang D,Fu J,Hou R,Liu K,Jonas JB,Wang H,Chen W,Li Z,Sang J,Zhang Z,Liu S,Cao Y,Xie X,Ren R,Lu Q,Weinreb RN,Wang N

更新日期：2014-04-15 00:00:00

abstract：PURPOSE:To determine the mechanism of reattachment of harvested human retinal pigment epithelium (RPE) to RPE-derived extracellular matrix and Bruch's membrane. METHODS:Confluent first-to third-passage human RPE were harvested from tissue culture and plated onto RPE-derived extracellular matrix or human Bruch's membra...

journal_title：Investigative ophthalmology & visual science

authors： Ho TC,Del Priore LV

更新日期：1997-05-01 00:00:00

abstract：Purpose:This study is to investigate the corneal anomaly caused by excess transforming growth factor-α (TGF-α) during mouse development. Methods:Bitransgenic KeraRT/TGF-α mice, generated via cross-mating tetO-TGF-α and KeraRT mice, were induced to overexpress TGF-α by doxycycline commencing at embryonic day 0 or postn...

journal_title：Investigative ophthalmology & visual science

authors： Zhang L,Yuan Y,Yeh LK,Dong F,Zhang J,Okada Y,Kao WWY,Liu CY,Zhang Y

更新日期：2020-07-01 00:00:00

abstract：PURPOSE:Age-related nuclear cataracts involve denaturation and aggregation of intracellular proteins. We have documented age-dependent changes in membrane transport in the mouse lens to see what might initiate changes in the intracellular milieu. METHODS:Microelectrode-based intracellular impedance studies of intact l...

journal_title：Investigative ophthalmology & visual science

authors： Gao J,Wang H,Sun X,Varadaraj K,Li L,White TW,Mathias RT

更新日期：2013-11-01 00:00:00

abstract：PURPOSE:To examine retinal function using the full-field electroretinogram (ERG) during and after acute intraocular pressure (IOP) elevation in wild-type mice. METHODS:IOP was elevated by anterior chamber cannulation in wild-type C57/BL6 mice. The pressure-function relationship was determined by IOP elevation in steps...

journal_title：Investigative ophthalmology & visual science

authors： Kong YX,Crowston JG,Vingrys AJ,Trounce IA,Bui VB

更新日期：2009-12-01 00:00:00

abstract：PURPOSE:To investigate the interobserver agreement for the detection of optical coherence tomography (OCT) features of disease activity in patients with neovascular age-related macular degeneration (nAMD). METHODS:This was a cross-sectional agreement study in which grading of OCT line scans from patients with nAMD was...

journal_title：Investigative ophthalmology & visual science

authors： Patel PJ,Browning AC,Chen FK,Da Cruz L,Tufail A

更新日期：2009-11-01 00:00:00

abstract：Purpose:Rose bengal (RB)-photosensitized protein crosslinking has been proposed for several applications in the eye. This study identifies oxygen-dependent and oxygen-independent mechanistic pathways in cornea for RB-photosensitized crosslinking to enhance its efficiency for ocular treatments. Methods:Rabbit corneas e...

journal_title：Investigative ophthalmology & visual science

authors： Wertheimer CM,Elhardt C,Kaminsky SM,Pham L,Pei Q,Mendes B,Afshar S,Kochevar IE

更新日期：2019-05-01 00:00:00

abstract：Purpose:Rapid identification of pathogens causing endophthalmitis may improve treatment outcomes through early administration of species-specific medication. The current study reports a new molecular application of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with Staphylococcus-specific molecular...

journal_title：Investigative ophthalmology & visual science

authors： Patel N,Miller D,Relhan N,Flynn HW Jr

更新日期：2017-08-01 00:00:00

abstract：:Adaptation of saccadic magnitude and vestibulo-ocular reflex gain (VOR) was examined in six monkeys that had undergone a tenectomy of the medial and lateral recti of one eye. After the tenectomy, when the normal eye was patched, a conjugate increase in saccadic magnitude and VOR was observed in both eyes. Subsequent u...

journal_title：Investigative ophthalmology & visual science

authors： Snow R,Hore J,Vilis T

更新日期：1985-07-01 00:00:00

abstract：PURPOSE:To determine whether complement-derived SC5b-9, the soluble nonlytic-fluid phase of the membrane attack complex, can be generated in normal human corneas when they are injured with lipopolysaccharide (LPS), ribitol teichoic acid (RTA) immune complexes, acid, or alkali. METHODS:The experimental cornea of each d...

journal_title：Investigative ophthalmology & visual science

authors： Mondino BJ,Chou HJ,Sumner HL

更新日期：1996-07-01 00:00:00

abstract：PURPOSE:To investigate whether oxygen stresses experienced in retinopathy of prematurity (ROP) trigger signaling through reactive oxygen species (ROS) and whether the Janus kinase-signal transducer and activator of transcription (JAK/STAT) pathway lead to intravitreous neovascularization (IVNV) in an oxygen-induced ret...

journal_title：Investigative ophthalmology & visual science

authors： Byfield G,Budd S,Hartnett ME

更新日期：2009-07-01 00:00:00

abstract：Purpose:To assess interocular delays in amblyopes with stereopsis and to evaluate the relationship between interocular delays and the clinical characteristics. Methods:Twenty amblyopes with stereopsis (median, 400 arcseconds) and 20 controls with normal or corrected to normal visual acuity (≤0 logMAR) and normal stere...

journal_title：Investigative ophthalmology & visual science

authors： Wu Y,Reynaud A,Tao C,Mao Y,He Z,Zhou J,Hess RF

更新日期：2020-03-09 00:00:00

abstract：PURPOSE:To investigate the relationship between ornithine-delta-aminotransferase (OAT) deficiency and ornithine accumulation and the specific degeneration of retinal pigment epithelial (RPE) cells in gyrate atrophy. METHODS:Human RPE cells, human hepatoma cells, and human fibroblast cells were treated with 5-fluoromet...

journal_title：Investigative ophthalmology & visual science

authors： Ueda M,Masu Y,Ando A,Maeda H,Del Monte MA,Uyama M,Ito S

更新日期：1998-04-01 00:00:00

abstract：PURPOSE:Many retinal imaging instruments use infrared wavelengths to reduce the risk of light damage. However, we have discovered that exposure to infrared illumination causes a long-lasting reduction in infrared autofluorescence (IRAF). We have characterized the dependence of this effect on radiant exposure and invest...

journal_title：Investigative ophthalmology & visual science

authors： Masella BD,Williams DR,Fischer WS,Rossi EA,Hunter JJ

更新日期：2014-05-20 00:00:00

abstract：PURPOSE:To explore changes in corneal sensitivity that develop in patients with dry eye and the relationship between sensibility and severity of the dry eye disease. METHODS:Experiments were performed in 44 patients with dry eye and 42 healthy individuals. Corneal sensitivity was measured with the Belmonte noncontact ...

journal_title：Investigative ophthalmology & visual science

authors： Bourcier T,Acosta MC,Borderie V,Borrás F,Gallar J,Bury T,Laroche L,Belmonte C

更新日期：2005-07-01 00:00:00

abstract：:The microvascular permeability response of the guinea pig conjunctiva to sulfidopeptide leukotrienes (LTs) was quantified as extravasation of radiolabeled bovine serum albumin. The LTs were potent inducers of increased microvascular permeability, with relative potencies LTE4 greater than or equal to LTD4 greater than ...

journal_title：Investigative ophthalmology & visual science

authors： Gary RK Jr,Woodward DF,Nieves AL,Williams LS,Gleason JG,Wasserman MA

更新日期：1988-01-01 00:00:00

abstract：PURPOSE:Human adenovirus species D type 19 (HAdV-D19) has been associated with epidemic keratoconjunctivitis (EKC), a highly inflammatory infection of the ocular surface. Confusion exists regarding the origins of HAdV-D19. The prototype virus (HAdV-D19p) does not cause EKC, while a virus identified later with the ident...

journal_title：Investigative ophthalmology & visual science

authors： Zhou X,Robinson CM,Rajaiya J,Dehghan S,Seto D,Jones MS,Dyer DW,Chodosh J

更新日期：2012-05-14 00:00:00

abstract：PURPOSE:To assess dynamic interactions of eye and head movements during return-sweep saccades (RSS) when reading with single-vision (SVL) versus progressive-addition (PAL) lenses in a simulated computer-based business environment. METHODS:Horizontal eye and head movements were recorded objectively and simultaneously a...

journal_title：Investigative ophthalmology & visual science

authors： Han Y,Ciuffreda KJ,Selenow A,Ali SR

更新日期：2003-04-01 00:00:00

abstract：:We tested the state of retinal correspondence at different positions in the visual field of ten observers with strabismic and/or anisometropic amblyopia, four strabismic subjects with alternating fixation and three normal controls. Correspondence was evaluated by the subjective displacement of dichoptic stimuli; to es...

journal_title：Investigative ophthalmology & visual science

authors： Sireteanu R,Fronius M

更新日期：1989-09-01 00:00:00

abstract：:Hypothesis-driven science is expected to result in a continuum of studies and findings along a discrete path. By comparison, serendipity can lead to new directions that branch into different paths. Herein, I describe a diverse series of findings that were motivated by hypotheses, but driven by serendipity. I summarize...

journal_title：Investigative ophthalmology & visual science

authors： Fischer AJ

更新日期：2011-09-29 00:00:00

abstract：PURPOSE:To investigate the effects of lomerizine, a Ca2+ antagonist, on the ocular tissue circulation in rabbits and on the circulation in the optic nerve head (ONH) and choroid in healthy volunteers. METHODS:Lomerizine (0.1 [n=10] or 0.3 [n=11] mg/kg) or vehicle solution (n=11) was injected intravenously in urethane-...

journal_title：Investigative ophthalmology & visual science

authors： Tamaki Y,Araie M,Fukaya Y,Nagahara M,Imamura A,Honda M,Obata R,Tomita K

更新日期：2003-11-01 00:00:00

abstract：PURPOSE:Excitotoxicity has been proposed to play a pivotal role in retinal damage, but the mechanisms that underlie retinal damage are not clearly understood. In this study, the role of matrix metalloproteinases in excitotoxin-mediated retinal damage was investigated. METHODS:KA, CNQX (6-cyano-7-nitroquinoxaline-2,3,-...

journal_title：Investigative ophthalmology & visual science

authors： Zhang X,Cheng M,Chintala SK

更新日期：2004-07-01 00:00:00

abstract：PURPOSE:Diabetic retinopathy is shown to share many similarities with chronic inflammatory disease, and in diabetes, accelerated apoptosis of retinal capillary cells is evident before histopathology can be seen. The purpose of this study was to examine the effect of interleukin (IL)-1beta on capillary cell apoptosis in...

journal_title：Investigative ophthalmology & visual science

authors： Kowluru RA,Odenbach S

更新日期：2004-11-01 00:00:00

abstract：PURPOSE:To assess the comparative accuracy of potential screening tests for open angle glaucoma (OAG). METHODS:Medline, Embase, Biosis (to November 2005), Science Citation Index (to December 2005), and The Cochrane Library (Issue 4, 2005) were searched. Studies assessing candidate screening tests for detecting OAG in ...

journal_title：Investigative ophthalmology & visual science

authors： Mowatt G,Burr JM,Cook JA,Siddiqui MA,Ramsay C,Fraser C,Azuara-Blanco A,Deeks JJ,OAG Screening Project.

更新日期：2008-12-01 00:00:00

abstract：:Wound healing in the eye may lead to undesirable sequelae such as proliferative vitreoretinopathy and scarring of glaucoma filtering fistulas. We therefore sought to manipulate in vitro two key wound healing processes--cell proliferation and contraction of extracellular matrices--using vitamin A (VA), retinoic acid (R...

journal_title：Investigative ophthalmology & visual science

authors： Kim RY,Stern WH

更新日期：1990-06-01 00:00:00

abstract：PURPOSE:Recent evidence suggests that platelets play a major role in ischemia-reperfusion injury, not only through thrombus formation but also through participation in inflammatory reactions with leukocytes. This study was designed to investigate the contribution of platelets in leukocyte recruitment to inflamed region...

journal_title：Investigative ophthalmology & visual science

authors： Nishijima K,Kiryu J,Tsujikawa A,Miyamoto K,Honjo M,Tanihara H,Nonaka A,Yamashiro K,Katsuta H,Miyahara S,Honda Y,Ogura Y

更新日期：2004-03-01 00:00:00

abstract：PURPOSE:To gain better understanding of the relationship between abnormalities detected by the multifocal VEP (mfVEP) compared with those detected by static achromatic, automated perimetry in patients with glaucoma. METHODS:Fifty patients were studied who had open-angle glaucoma that met the following criteria: (1) a ...

journal_title：Investigative ophthalmology & visual science

authors： Hood DC,Thienprasiddhi P,Greenstein VC,Winn BJ,Ohri N,Liebmann JM,Ritch R

更新日期：2004-02-01 00:00:00