HPTLC-aptastaining - Innovative protein detection system for high-performance thin-layer chromatography.

Abstract:

:Protein analysis using high-performance thin-layer chromatography (HPTLC) is not commonly used but can complement traditional electrophoretic and mass spectrometric approaches in a unique way. Due to various detection protocols and possibilities for hyphenation, HPTLC protein analysis is a promising alternative for e.g., investigating posttranslational modifications. This study exemplarily focused on the investigation of lysozyme, an enzyme which is occurring in eggs and technologically added to foods and beverages such as wine. The detection of lysozyme is mandatory, as it might trigger allergenic reactions in sensitive individuals. To underline the advantages of HPTLC in protein analysis, the development of innovative, highly specific staining protocols leads to improved sensitivity for protein detection on HPTLC plates in comparison to universal protein derivatization reagents. This study aimed at developing a detection methodology for HPTLC separated proteins using aptamers. Due to their affinity and specificity towards a wide range of targets, an aptamer based staining procedure on HPTLC (HPTLC-aptastaining) will enable manifold analytical possibilities. Besides the proof of its applicability for the very first time, (i) aptamer-based staining of proteins is applicable on different stationary phase materials and (ii) furthermore, it can be used as an approach for a semi-quantitative estimation of protein concentrations.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Morschheuser L,Wessels H,Pille C,Fischer J,Hünniger T,Fischer M,Paschke-Kratzin A,Rohn S

doi

10.1038/srep26665

subject

Has Abstract

pub_date

2016-05-25 00:00:00

pages

26665

issn

2045-2322

pii

srep26665

journal_volume

6

pub_type

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