Abstract：
:A major goal of biology is to develop a quantitative ligand-binding assay that does not involve the use of radioactivity. Existing fluorescence-based assays have a serious drawback due to fluorescence quenching that accompanies the binding of fluorescently-labeled ligands to their receptors. This limitation of existing fluorescence-based assays prevents the number of cellular receptors under investigation from being accurately measured. We have developed a method where FITC-labeled proteins bound to a cell surface are proteolyzed extensively to eliminate fluorescence quenching and then the fluorescence of the resulting sample is compared to that of a known concentration of the proteolyzed FITC-protein employed. This step enables the number of cellular receptors to be measured quantitatively. We expect that this method will provide researchers with a viable alternative to the use of radioactivity in ligand binding assays.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Breen CJ,Raverdeau M,Voorheis HPdoi
10.1038/srep25769subject
Has Abstractpub_date
2016-05-10 00:00:00pages
25769issn
2045-2322pii
srep25769journal_volume
6pub_type
杂志文章abstract：:The Yiyuan hominin fossil site is one of the few localities in China where a partial skullcap and several loose teeth of Homo erectus have been discovered. Yiyuan was previously assigned broadly to the Middle Pleistocene by biostratigraphical correlation and ESR/U-series dating. Here, we report the first application o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43401-5
更新日期：2019-05-06 00:00:00
abstract：:Temporal fluctuation of neural activity in the brain has an important function in optimal information processing. Spontaneous activity is a source of such fluctuation. The distribution of excitatory postsynaptic potentials (EPSPs) between cortical pyramidal neurons can follow a log-normal distribution. Recent studies ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49286-8
更新日期：2019-09-04 00:00:00
abstract：:Society's increasing interactions with technology are creating extensive "digital traces" of our collective human behavior. These new data sources are fuelling the rapid development of the new field of computational social science. To investigate user attention to the Hurricane Sandy disaster in 2012, we analyze data ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03141
更新日期：2013-11-05 00:00:00
abstract：:Burkholderia terrae BS001 has previously been found to be able to disperse along with growing fungal hyphae in soil, with the type-3 secretion system having a supportive role in this movement. In this study, we focus on the role of two motility- and adherence-associated appendages, i.e. type-4 pili (T4P) and flagella....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02959-8
更新日期：2017-06-07 00:00:00
abstract：ABSTARCT:The relationship between chronic kidney disease (CKD), which is defined by declined estimated glomerular filtration rate (eGFR) and/or proteinuria, and asymptomatic intracranial arterial stenosis (ICAS) is largely unknown. We conducted a population-based, cross-sectional study by recruiting 5209 participants f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04549-0
更新日期：2017-07-04 00:00:00
abstract：:Caveolins are essential structural proteins driving the formation of caveolae, specialized invaginations of the plasma membrane. Loss of Caveolin-1 (Cav1) function in mice causes distinct neurological phenotypes leading to impaired motor control, however, the underlying developmental mechanisms are largely unknown. In...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73429-x
更新日期：2020-10-05 00:00:00
abstract：:Dopamine is an important neurotransmitter that plays a key role in a wide range of both locomotive and cognitive functions in humans. Disturbances on the dopaminergic system cause, among others, psychosis, Parkinson's disease and Huntington's disease. Antipsychotics are drugs that interact primarily with the dopamine ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78446-4
更新日期：2020-12-09 00:00:00
abstract：:Here we present a set of measurements using Differential Scanning Fluorimetry (DSF) as an inexpensive, high throughput screening method to investigate the folding of silk protein molecules as they abandon their first native melt conformation, dehydrate and denature into their final solid filament conformation. Our fir...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05625
更新日期：2014-07-09 00:00:00
abstract：:The ability to avoid predators is crucial to wild prey animals' survival. Potential danger is signalled, among others, by the presence of predator scents. These odors are used in research both to trigger and to study fear reactions in laboratory animals; they are also employed as repellents against pest rodent species...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27054-4
更新日期：2018-06-21 00:00:00
abstract：:SHH Medulloblastoma (SHH-MB) is a pediatric brain tumor characterized by an inappropriate activation of the developmental Hedgehog (Hh) signaling. SHH-MB patients treated with the FDA-approved vismodegib, an Hh inhibitor that targets the transmembrane activator Smoothened (Smo), have shown the rapid development of dru...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44079
更新日期：2017-03-09 00:00:00
abstract：:The programmed induction of meiotic DNA double-strand breaks (DSBs) by the evolutionarily conserved SPO-11 protein, which is structurally related to archaeal Topo VIA topoisomerases, triggers meiotic recombination. Identification of several meiosis-specific factors that are required for SPO-11-mediated DSB formation r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02641-z
更新日期：2017-05-24 00:00:00
abstract：:High-throughput single-stranded DNA sequencing (ssDNA-seq) of cell-free DNA from plasma and other bodily fluids is a powerful method for non-invasive prenatal testing, and diagnosis of cancers and other diseases. Here, we developed a facile ssDNA-seq method, which exploits a novel template-switching activity of thermo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09064-w
更新日期：2017-08-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62599-3
更新日期：2020-04-14 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-63289-w
更新日期：2020-04-07 00:00:00
abstract：:Insects are ectothermal animals that are constrained in their survival and reproduction by external temperature fluctuations which require either active avoidance of or movement towards a given heat source. In Drosophila, different thermoreceptors and neurons have been identified that mediate temperature sensation to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80103-9
更新日期：2021-01-08 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13754-w
更新日期：2017-10-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39768-0
更新日期：2019-02-27 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22027-z
更新日期：2018-02-23 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42302
更新日期：2017-02-08 00:00:00
abstract：:The intention of this observational study is to show the significant impact of comorbidities and smoking on the outcome in aneurysmal subarachnoid hemorrhage (SAH). During this observational study 203 cases of treatment of ruptured intracranial aneurysms were analyzed. We examined and classified prospectively the 12 m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30878-9
更新日期：2018-08-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14518-2
更新日期：2017-10-26 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17914-w
更新日期：2017-12-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08811-3
更新日期：2017-08-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80453-4
更新日期：2021-01-18 00:00:00
abstract：:We found a novel spontaneous mouse mutant with depigmentation in the ventral body, which we called White Spotting (WS) mouse. Genetic investigation revealed deletion of a > 1.2-Mb genomic region containing nine genes (Kit, Kdr, Srd5a3, Tmeme165, Clock, Pdcl2, Nmu, Exoc1, and Cep135). We designated this mutant allele K...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13632
更新日期：2015-09-08 00:00:00
abstract：:This study aimed to define the width and length of the dental arch in 12-year-old Vietnamese children, and to elucidate differences between genders and among ethnic groups. A cross-sectional study was conducted in 4565 12 years-old children from the 4 major ethnic groups in Vietnam (Kinh, Muong, Thai, and Tay), with a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39710-4
更新日期：2019-02-28 00:00:00
abstract：:Although low socioeconomic status (SES) is related to poor glycemic control, the underlying mechanisms remain unclear. We examined potentially modifiable factors involved in the association between low SES and poor glycemic control using data from the baseline survey of a multicenter, prospective cohort study. Five hu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63253-8
更新日期：2020-04-21 00:00:00
abstract：:Traumatic brain injury (TBI) is a risk factor for Alzheimer's disease (AD), although the mechanisms contributing to this increased risk are unknown. Insulin resistance is an additional risk factor for AD whereby decreased insulin signaling increases synaptic sensitivity to amyloid beta (Aβ) and tau. Considering this, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44635-z
更新日期：2019-06-03 00:00:00
abstract：:We propose a quantum secure group communication protocol for the purpose of sharing the same message among multiple authorized users. Our protocol can remove the need for key management that is needed for the quantum network built on quantum key distribution. Comparing with the secure quantum network based on BB84, we...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21743-w
更新日期：2018-03-01 00:00:00
abstract：:It is highly important to understand the heterogeneous wettability properties of porous media for enhanced oil recovery (EOR). However, wettability measurements are still challenging in directly investigating the wettability of porous media. In this paper, we propose a multidimensional nuclear magnetic resonance (NMR)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31803-w
更新日期：2018-09-07 00:00:00
