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A continuous sirtuin activity assay without any coupling to enzymatic or chemical reactions.

Abstract：

:Sirtuins are NAD(+) dependent lysine deacylases involved in many regulatory processes such as control of metabolic pathways, DNA repair and stress response. Modulators of sirtuin activity are required as tools for uncovering the biological function of these enzymes and as potential therapeutic agents. Systematic discovery of such modulators is hampered by the lack of direct and continuous activity assays. The present study describes a novel continuous assay based on the increase of a fluorescence signal subsequent to sirtuin mediated removal of a fluorescent acyl chain from a modified TNFα-derived peptide. This substrate is well recognized by human sirtuins 1-6 and represents the best sirtuin 2 substrate described so far with a kcat/KM-value of 176 000 M(-1)s(-1). These extraordinary substrate properties allow the first determination of Ki-values for the specific Sirt2 inhibitory peptide S2iL5 (600 nM) and for the quasi-universal sirtuin inhibitor peptide thioxo myristoyl TNFα (80 nM).

journal_name

Sci Rep

journal_title

Scientific reports

authors

Schuster S,Roessler C,Meleshin M,Zimmermann P,Simic Z,Kambach C,Schiene-Fischer C,Steegborn C,Hottiger MO,Schutkowski M

doi

10.1038/srep22643

subject

Has Abstract

pub_date

2016-03-04 00:00:00

pages

22643

issn

2045-2322

pii

srep22643

journal_volume

6

pub_type

杂志文章

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