Abstract:
:The resistance-nodulation-cell division (RND)-type xenobiotic efflux system plays a major role in the multidrug resistance of gram-negative bacteria. The only constitutively expressed RND system of Escherichia coli consists of the inner membrane transporter AcrB, the membrane fusion protein AcrA, and the outer membrane channel TolC. The latter two components are shared with another RND-type transporter AcrD, whose expression is induced by environmental stimuli. Here, we demonstrate how RND-type ternary complexes, which span two membranes and the cell wall, form in vivo. Total internal reflection fluorescence (TIRF) microscopy revealed that most fluorescent foci formed by AcrB fused to green fluorescent protein (GFP) were stationary in the presence of TolC but showed lateral displacements when tolC was deleted. The fraction of stationary AcrB-GFP foci decreased with increasing levels of AcrD. We propose that the AcrB-containing complex becomes unstable upon the induction of AcrD, which presumably replaces AcrB, a process we call "transporter exchange." This instability is suppressed by AcrB-specific substrates, suggesting that the ternary complex is stabilised when it is in action. These results suggest that the assembly of the RND-type efflux system is dynamically regulated in response to external stimuli, shedding new light on the adaptive antibiotic resistance of bacteria.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yamamoto K,Tamai R,Yamazaki M,Inaba T,Sowa Y,Kawagishi Idoi
10.1038/srep21909subject
Has Abstractpub_date
2016-02-26 00:00:00pages
21909issn
2045-2322pii
srep21909journal_volume
6pub_type
杂志文章abstract::This paper reports on 3D phase field simulations of IMC growth in Co/Sn and Cu/Sn solder systems. In agreement with experimental micrographs, we obtain uniform growth of the CoSn3 phase in Co/Sn solder joints and a non-uniform wavy morphology for the Cu6Sn5 phase in Cu/Sn solder joints. Furthermore, simulations were p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51179-9
更新日期:2019-10-16 00:00:00
abstract::Sludge bulking is a major problem that restricts the development of the activated sludge process. The microbial community responsible for sludge bulking varies depending on water quality and operational conditions. This study analysed the microbial community of bulking sludge in oxidation ditch with ultra-low sludge l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50086-3
更新日期:2019-09-23 00:00:00
abstract::Increased fibroblast growth factor 21 (FGF21) levels have been found in patients with metabolic syndrome (MetS). MetS is also associated with cognitive decline. However, the correlation between FGF21 and cognitive decline in elderly and nonelderly MetS patients has not been investigated. 116 non-elderly patients (age ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23550-9
更新日期:2018-03-26 00:00:00
abstract::Thomas Kent was an Irish rebel who was executed by British forces in the aftermath of the Easter Rising armed insurrection of 1916 and buried in a shallow grave on Cork prison's grounds. In 2015, ninety-nine years after his death, a state funeral was offered to his living family to honor his role in the struggle for I...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/srep41529
更新日期:2017-01-30 00:00:00
abstract::Elevated prostaglandin E2 (PGE2) levels are observed in colorectal cancer (CRC) patients, and this increase is associated with poor prognosis. Increased synthesis of PGE2 in CRC has been shown to occur through COX-2-dependent mechanisms; however, loss of the PGE2-catabolizing enzyme, 15-hydroxyprostaglandin dehydrogen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41862-2
更新日期:2019-04-01 00:00:00
abstract::The discovery of the insertion of IbT-DNA1 and IbT-DNA2 into the cultivated (hexaploid) sweetpotato [Ipomoea batatas (L.) Lam.] genome constitutes a clear example of an ancient event of Horizontal Gene Transfer (HGT). However, it remains unknown whether the acquisition of both IbT-DNAs by the cultivated sweetpotato oc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48691-3
更新日期:2019-08-29 00:00:00
abstract::A wide range of human diseases is associated with mutations that, destabilizing proteins native state, promote their aggregation. However, the mechanisms leading from folded to aggregated states are still incompletely understood. To investigate these mechanisms, we used a combination of NMR spectroscopy and molecular ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25559
更新日期:2016-05-06 00:00:00
abstract::The Longwangzhuang pluton is a typical example of Paleoproterozoic A-type granite intrusions at the southern margin of the North China Craton. This pluton is composed of arfvedsonite granite and minor aegirine-augite granites. Samples from both granite types display similar zircon U-Pb ages with 207U-206Pb ages of 161...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56820-1
更新日期:2020-01-09 00:00:00
abstract::Subduction zones facilitate chemical exchanges between Earth's deep interior and volcanism that affects habitability of the surface environment. Lavas erupted at subduction zones are oxidized and release volatile species. These features may reflect a modification of the oxidation state of the sub-arc mantle by hydrous...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09626-y
更新日期:2017-09-04 00:00:00
abstract::Balancing stomata-dependent CO2 assimilation and transpiration is a key challenge for increasing crop productivity and water use efficiency under drought stress for sustainable crop production worldwide. Here, we show that cucumber and luffa plants with luffa as rootstock have intrinsically increased water use efficie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20212
更新日期:2016-02-02 00:00:00
abstract::Cells operate in noisy molecular environments via complex regulatory networks. It is possible to understand how molecular counts are related to noise in specific networks, but it is not generally clear how noise relates to network complexity, because different levels of complexity also imply different overall number o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20214
更新日期:2016-02-08 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-44364-3
更新日期:2019-06-06 00:00:00
abstract::Mutations at positions 70 and/or 91 in the core protein of genotype-1b, hepatitis C virus (HCV) are associated with hepatocellular carcinoma (HCC) risk in Asian patients. To evaluate this in a US population, the relationship between the percentage of 70 and/or 91 mutant HCV quasispecies in baseline serum samples of ch...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/srep27025
更新日期:2016-06-01 00:00:00
abstract::Lipid lateral diffusion in membrane bilayers is a fundamental process exploited by cells to enable complex protein structural and dynamic reorganizations. For its importance, lipid mobility in both cellular and model bilayers has been extensively investigated in recent years, especially through the application of time...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37814-x
更新日期:2019-02-06 00:00:00
abstract::Bony defects are a common problem in musculoskeletal surgery. Replacement with autologous bone grafts is limited by availability of transplant material. Sterilized cancellous bone, while being osteoconductive, has limited osteoinductivity. Nanofiber scaffolds are currently used for several purposes due to their capabi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66281-6
更新日期:2020-06-12 00:00:00
abstract::Unnatural chiral α-amino acids are widely used in fields of organic chemistry, biochemistry and medicinal chemistry, and their synthesis has attracted extensive attention. Although the asymmetric synthesis provides some efficient protocols, noble and elaborate catalysts, ligands and additives are usually required whic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26161
更新日期:2016-05-17 00:00:00
abstract::This study was aimed to explore the bidirectional association between depression and peptic ulcers. The ≥20-year-old participants of the Korean National Health Insurance Service-National Sample Cohort from 2002 to 2013 were included in the study. In study I, 30,306 depression patients were 1:4 matched with 121,224 con...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58783-0
更新日期:2020-02-04 00:00:00
abstract::The accurate prediction of the impact of an amino acid substitution on the thermal stability of a protein is a central issue in protein science, and is of key relevance for the rational optimization of various bioprocesses that use enzymes in unusual conditions. Here we present one of the first computational tools to ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23257
更新日期:2016-03-18 00:00:00
abstract::Heart failure is the most common cause of morbidity and hospitalization in the western civilization. Protein phosphatases play a key role in the basal cardiac contractility and in the responses to β-adrenergic stimulation with type-1 phosphatase (PP-1) being major contributor. We propose here that formation of transie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36267-6
更新日期:2018-12-07 00:00:00
abstract::The identification of the most influential individuals in structured populations is an important research question, with many applications across the social and natural sciences. Here, we study this problem in evolutionary populations on static networks, where invading cheaters can lead to the collapse of cooperation....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59020-4
更新日期:2020-02-10 00:00:00
abstract::Many human introns carry out a function, in the sense that they are critical to maintain normal cellular activity. Their identification is fundamental to understanding cellular processes and disease. However, being noncoding elements, such functional introns are poorly predicted based on traditional approaches of sequ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04476-0
更新日期:2017-06-23 00:00:00
abstract::Proteins are of interest in nano-bio electronic devices due to their versatile structures, exquisite functionality and specificity. However, quantum transport measurements produce conflicting results due to technical limitations whereby it is difficult to precisely determine molecular orientation, the nature of the mo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39792
更新日期:2017-01-04 00:00:00
abstract::OVGP1 is the major non-serum glycoprotein in the oviduct fluid at the time of fertilization and early embryo development. Its activity differs among species. Here, we show that the C-terminal region of recombinant OVGP1 regulates its binding to the extracellular zona pellucida and affects its activity during fertiliza...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32556
更新日期:2016-09-07 00:00:00
abstract::The novel neuropeptide spexin (SPX) was discovered to activate galanin receptor 2 (GALR2) and 3 (GALR3) but not galanin receptor 1 (GALR1). Although GALR2 is known to display a function, particularly in anxiety, depression, and appetite regulation, the further determination of its function would benefit from a more st...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21453
更新日期:2016-02-24 00:00:00
abstract::Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1. However, a systematic comparison of the binding specificities of anti-MUC1 antibodies was not previously conducted. Her...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53052-1
更新日期:2019-11-12 00:00:00
abstract::A new method combining aqueous solution printing with UV Laser crystallization (UVLC) and post annealing is developed to deposit highly transparent and conductive Aluminum doped Zinc Oxide (AZO) films. This technique is able to rapidly produce large area AZO films with better structural and optoelectronic properties t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15517
更新日期:2015-10-30 00:00:00
abstract::According to the Source Monitoring Framework, the origin of a memory is remembered through the retrieval of specific features (e.g. perceptive, sensitive, affective signals). In two source discrimination tasks, we studied the ability of cuttlefish to remember the modality in which an item had been presented several ho...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62335-x
更新日期:2020-03-25 00:00:00
abstract::The three-dimensional construction of arrays of functional molecules on an electrode surface, such as organic semiconductors and redox-active molecules, is a considerable challenge in the fabrication of sophisticated junctions for molecular devices. In particular, well-defined organic layers with precise molecular gra...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11122
更新日期:2015-06-09 00:00:00
abstract::We demonstrated an abnormal double-peak (annular shaped) energy deposition through dual-wavelength synthesis of the fundamental frequency (ω) and the second-harmonic frequency (2ω) of a femtosecond (fs) Ti:sapphire laser. The annular shaped distribution of the dual-wavelength fs laser was confirmed through real beam s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16374-6
更新日期:2017-12-11 00:00:00
abstract::Mycobacterium marinum (M. marinum) is a slowly growing nontuberculous mycobacterium. The incidence of M. marinum infections in Denmark is unknown. We conducted a retrospective nationwide study including all culture confirmed cases of M. marinum from 2004 to 2017 in Denmark. All available medical records were reviewed....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24702-7
更新日期:2018-04-30 00:00:00