Abstract：
:Lipid lateral diffusion in membrane bilayers is a fundamental process exploited by cells to enable complex protein structural and dynamic reorganizations. For its importance, lipid mobility in both cellular and model bilayers has been extensively investigated in recent years, especially through the application of time-resolved, fluorescence-based, optical microscopy techniques. However, one caveat of fluorescence techniques is the need to use dye-labeled variants of the lipid of interest, thus potentially perturbing the structural and dynamic properties of the native species. Generally, the effect of the dye/tracer molecule is implicitly assumed to be negligible. Nevertheless, in view of the widespread use of optically modified lipids for studying lipid bilayer dynamics, it is highly desirable to well assess this point. Here, fluorescence correlation spectroscopy (FCS) and molecular dynamics (MD) simulations have been combined together to uncover subtle structural and dynamic effects in DOPC planar membranes enriched with a standard Rhodamine-labeled lipid. Our findings support a non-neutral role of the dye-labeled lipids in diffusion experiments, quantitatively estimating a decrease in lipid mobility of up to 20% with respect to the unlabeled species. Moreover, results highlight the existing interplay between dye concentration, lipid lateral diffusion and membrane permeability, thus suggesting possible implications for future optical microscopy studies of biophysical processes occurring at the membrane level.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Jan Akhunzada M,D'Autilia F,Chandramouli B,Bhattacharjee N,Catte A,Di Rienzo R,Cardarelli F,Brancato Gdoi
10.1038/s41598-018-37814-xsubject
Has Abstractpub_date
2019-02-06 00:00:00pages
1508issue
1issn
2045-2322pii
10.1038/s41598-018-37814-xjournal_volume
9pub_type
杂志文章abstract：:The lunar cycle is believed to strongly influence the vertical distribution of many oceanic taxa, with implications for the foraging behaviour of nocturnal marine predators. Most studies to date testing lunar effects on foraging have focused on predator activity at-sea, with some birds and marine mammals demonstrating...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01312-3
更新日期：2017-05-03 00:00:00
abstract：:During anaerobic digestion (AD) process, process parameters e.g., VFA, pH, COD removal … and kinetic parameters e.g., hydrolysis rate, lag phase and methane production potential… are the important indicator for illustrating AD process performance, however, the AD process performance based on these parameters remains p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09977-6
更新日期：2017-08-24 00:00:00
abstract：:The aim of this study was to estimate the incidence of T2DM in three population groups: rural, rural-to-urban migrants and urban dwellers. Data from the PERU MIGRANT Study was analysed. The baseline assessment was conducted in 2007-2008 using a single-stage random sample and further follow-up was undertaken in 2015-16...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23812-6
更新日期：2018-04-03 00:00:00
abstract：:Arctic and alpine environments present extreme, but different, challenges to survival. We therefore studied the ecological adaptation of the arctic-alpine fritillary Boloria napaea in northern Sweden and compared these results with the eastern Alps. Using mark-release-recapture, we analysed phenology, mobility, activi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40508-7
更新日期：2019-03-07 00:00:00
abstract：:Here we show that electrical tuning of the sensitivity of chemitransistor sensors, namely field-effect-transistors (FETs) exploiting nano/mesostructured sensing materials, can be used to effectively address two chief problems of state-of-the-art gas sensors, specifically fabrication reliability and degradation by agin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01161
更新日期：2013-01-01 00:00:00
abstract：:Recently, slow earthquakes (slow EQ) have received much attention relative to understanding the mechanisms underlying large earthquakes and to detecting their precursors. Low-frequency earthquakes (LFE) are a specific type of slow EQ. In the present paper, we reveal the relevance of LFEs to the 11 March 2011 Great Toh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45765-0
更新日期：2019-06-27 00:00:00
abstract：:Aging-associated decrease in internal anal sphincter (IAS) tone (AADI) is a major contributor in the rectoanal incontinence (RI). To determine the pathogenesis of AADI, we investigated the effect of aging on GPCR activation and related downstream signaling. We particularly investigated two GPCRs that characterize IAS ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42894-4
更新日期：2019-05-01 00:00:00
abstract：:Resistance to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) has become the main clinical challenge of advanced lung cancer. This research aimed to explore the role of PARP1-mediated autophagy in the progression of TKI therapy. PARP1-mediated autophagy was evaluated in vitro by CCK-8 assay, cl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77908-z
更新日期：2020-12-01 00:00:00
abstract：:Climatic changes can induce geographic expansion and altitudinal shifts in the distribution of invasive species by offering more thermally suitable habitats. At the remote sub-Antarctic Kerguelen Islands, the predatory insect Merizodus soledadinus (Coleoptera: Carabidae), introduced in 1913, rapidly invaded coastal ha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57868-0
更新日期：2020-01-27 00:00:00
abstract：:The performances of two identical biofilters, filled with a new composite packing material (named CM-5) embedded with functional microorganisms or sterilized CM-5 without microorganisms, were investigated for H2S treatment. Running parameters in terms of microbial counts, pressure drops, and inlet and outlet H2S conce...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42241
更新日期：2017-02-13 00:00:00
abstract：:Amorphous silicon oxycarbide (SiOC) is of great technological interest. However, its atomic-level structure is not well understood. Using density functional theory calculations, we show that the clustering tendency of C atoms in SiOC is extremely sensitive to hydrogen (H): without H, the C-C interaction is attractive,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13051
更新日期：2015-08-13 00:00:00
abstract：:Oxidation of squalene (SQ) causes a decline in the nutritional value of SQ in foods, as well as an accumulation of SQ oxidation products in skin lipids which lead to adverse skin conditions. However, mechanistic insights as to how SQ is oxidized by different oxidation mechanisms have been limited, and thus effective m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27455-5
更新日期：2018-06-14 00:00:00
abstract：:Alkaline sulfide-rich hot springs provide a unique environment for microbial community and arsenic (As) biogeochemistry. In this study, a representative alkaline sulfide-rich hot spring, Zimeiquan in the Tengchong geothermal area, was chosen to study arsenic geochemistry and microbial community using Illumina MiSeq se...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25262
更新日期：2016-04-29 00:00:00
abstract：:Neuropathic pain is a complex, chronic pain state that often accompanies tissue damage, inflammation or injury of the nervous system. However the underlying molecular mechanisms still remain unclear. Here, we showed that CXCL12 and CXCR4 were upregulated in the dorsal root ganglion (DRG) after chronic compression of D...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05954-1
更新日期：2017-07-18 00:00:00
abstract：:The development and maturation of cortical circuits relies on the coordinated actions of long and short range axonal guidance cues. In this regard, the class 3 semaphorins and their receptors have been seen to be involved in the development and maturation of the hippocampal connections. However, although the role of m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19794-0
更新日期：2018-01-22 00:00:00
abstract：:The definition of a precise illumination region is essential in many applications where the electromagnetic field should be confined in some specific volume. By using conventional structures, it is difficult to achieve an adequate confinement distance (or volume) with negligible levels of radiation leakage beyond it. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37739
更新日期：2016-11-25 00:00:00
abstract：:Misfolding and aggregation of prion protein (PrP) causes neurodegenerative diseases like Creutzfeldt-Jakob disease (CJD) and scrapie. Besides the consensus that spontaneous conversion of normal cellular PrPC into misfolded and aggregating PrPSc is the central event in prion disease, an alternative hypothesis suggests ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39213-2
更新日期：2019-02-21 00:00:00
abstract：:PC4 is an abundant single-strand DNA binding protein that has been implicated in transcription and DNA repair. Here, we show that PC4 is involved in the cellular DNA damage response. To elucidate the role, we used the DT40 chicken B cell model, which produces clustered DNA lesions at Ig loci via the action of activati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28890
更新日期：2016-07-04 00:00:00
abstract：:The conservation of plant and animal genetic heritage is not a purpose in itself, but it represents the sine qua non condition for practicing a sustainable agriculture and to ensure nutrition and food security on long-term. Our research focused on identifying the areas with the richest genetic diversity of vegetables ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75413-x
更新日期：2020-10-28 00:00:00
abstract：:In this study, to fabricate stable floating photocatalytic spheres, facile alcohol solvothermal reduction was first employed to modify commercial TiO2 (P25) photocatalysts to harvest visible light and improve their performances for photodegrading phenol in seawater exciting by visible light. Floating photocatalytic sp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50457-w
更新日期：2019-09-25 00:00:00
abstract：:Two-dimensional (2D) porous carbon AC-SPN-3 possessing of amazing high micropore volume ratio of 83% and large surface area of about 1069 m(2) g(-1) is high-yield obtained by pyrolysis of natural waste Pistachio nutshells with KOH activation. The AC-SPN-3 has a curved 2D lamellar morphology with the thickness of each ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05545
更新日期：2014-07-02 00:00:00
abstract：:Creating and maintaining precipitates coherent with the host matrix, under service conditions is one of the most effective approaches for successful development of alloys for high temperature applications; prominent examples include Ni- and Co-based superalloys and Al alloys. While ferritic alloys are among the most i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11772
更新日期：2015-07-02 00:00:00
abstract：:Aging is a highly controlled biological process characterized by a progressive deterioration of various cellular activities. One of several hallmarks of aging describes a link to transcriptional alteration, suggesting that it may impact the steady-state mRNA levels. We analyzed the mRNA steady-state levels of polyCAG-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10692
更新日期：2015-06-02 00:00:00
abstract：:Propagation of oscillatory spike firing activity at specific frequencies plays an important role in distributed cortical networks. However, there is limited evidence for how such frequency-specific signals are induced or how the signal spectra of the propagating signals are modulated during across-layer (radial) and i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26054-8
更新日期：2018-05-16 00:00:00
abstract：:The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co2+ and Mn2+. Under these reaction conditions, the enzyme displayed Km and kcat values of 0.2364 ± 0.018 mM and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10932-8
更新日期：2017-09-06 00:00:00
abstract：:Partial thrombosis of the false lumen in uncomplicated type B aortic dissection (uTBAD) is associated with an increased late mortality risk. Whether the serum cortisol level can affect false lumen thrombosis in patients with uTBAD has not been well characterized. This study was performed on 87 patients with uTBAD. A c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19691-6
更新日期：2018-01-19 00:00:00
abstract：:Fitting Ordinary Differential Equation (ODE) models of signal transduction networks (STNs) to experimental data is a challenging problem. Computational parameter fitting algorithms simulate a model many times with different sets of parameter values until the simulated STN behaviour match closely with experimental data...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30118-0
更新日期：2018-08-03 00:00:00
abstract：:Childhood obesity and early rapid growth increase the risk for type 2 diabetes. Such early overnutrition can be modeled in mice by reducing litter size. We investigated the effects of early overnutrition and increased dietary fat intake on β cell function in Swiss Webster mice. On a moderate-fat diet, early overnutrit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39177-3
更新日期：2019-03-06 00:00:00
abstract：:Hydrocarbons fuel our economy. Furthermore, intermediate goods and consumer products are often hydrocarbon-based. Beside all the progress they made possible, hydrogen-containing substances can have severe detrimental effects on materials exposed to them. Hydrogen-assisted failure of iron alloys has been recognised mor...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60370-2
更新日期：2020-02-27 00:00:00
abstract：:Bacterial cells have characteristic spatial and temporal scales. For instance, Escherichia coli, the typical rod-shaped bacteria, always maintains a relatively constant cell width and cell division time. However, whether the external physical perturbation of cell width has an impact on cell division time remains large...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74778-3
更新日期：2020-10-20 00:00:00
