Abstract：
:A protocol for high frequency production of somatic embryos was worked out in pigeonpea, Cajanus cajan (L.) Millsp. The protocol involved sequential employment of embryogenic callus cultures, low density cell suspension cultures and a novel microdroplet cell culture system. The microdroplet cell cultures involved culture of a single cell in 10 μI of Murashige and Skoog's medium supplemented with phytohormones, growth factors and phospholipid precursors. By employing the microdroplet cell cultures, single cells in isolation were grown into cell clones which developed somatic embryos. Further, 2,4-dichlorophenoxyacetic acid, kinetin, polyethylene glycol, putrescine, spermine, spermidine, choline chloride, ethanolamine and LiCl were supplemented to the low density cell suspension cultures and microdroplet cell cultures to screen for their cell division and somatic embryogenesis activity. Incubation of callus or the inoculum employed for low density cell suspension cultures and microdroplet cell cultures with polyethylene glycol was found critical for induction of somatic embryogenesis. Somatic embryogenesis at a frequency of 1.19, 3.16 and 6.51 per 10(6) cells was achieved in the callus, low density cell suspension cultures and microdroplet cell cultures, respectively. Advantages of employing microdroplet cell cultures for high frequency production of somatic embryos and its application in genetic transformation protocols are discussed.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Kumar NU,Gnanaraj M,Sindhujaa V,Viji M,Manoharan Ksubject
Has Abstractpub_date
2015-09-01 00:00:00pages
600-10issue
9eissn
0019-5189issn
0975-1009journal_volume
53pub_type
杂志文章abstract：:Methanolic extract of P. amarus was found to have potential anti-oxidant activity as it could inhibit lipid peroxidation, and scavenge hydroxyl and superoxide radicals in vitro. The amount required for 50% inhibition of lipid peroxide formation was 104 microg/ml and the concentrations needed to scavenge hydroxyl and s...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-08-01 00:00:00
abstract：:Mouse liver was exposed in vivo to a continuous and unfocussed ultrasound of 875 KHz at 5, 10 or 15 W/cm2 for 300 sec spread over five days and excised on day 0, 1, 5 and 10 for studying the changes in Na+ and K+ reserves and Na-K-ATPase activity. At 5 and 10 W intensities the change in ionic concentrations and Na-K-A...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Supplementation of cyanate in rats caused a significant decrease in serum GSH and increase in calcium and phosphate level both in serum and lens. Consequently, these changes led to induce acidosis uremia in serum and hypercalcemia and hyperphosphatemia in lens which may be possible causing factor for cataract. ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Present investigation was undertaken to evaluate antihyperglycemic, antihyperlipidemic and antioxidant activities of Dihar, a polyherbal formulation containing drugs from eight different herbs viz., Syzygium cumini, Momordica charantia, Emblica officinalis, Gymnema sylvestre, Enicostemma littorale, Azadirachta indica,...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
abstract：:A simple dot-immunobinding assay (Dot-Iba) in nitrocellulose paper was developed for the detection of specific IgG antibody to Mycobacterium tuberculosis antigen 5 and mycobacterial antigen in cerebrospinal fluid of patients with tuberculous meningitis (TBM). The assay gave 77.1% sensitivity for the detection of IgG a...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:The present study is planned to assess the diagnostic relevance of silver stained nucleolar organizer regions (NORs) in discriminating benign from malignant lesions of the breast and to ascertain the interobserver variability in the enumeration of AgNOR counts. Forty four specimens of benign and malignant breast tissu...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:Leydig cells are the primary source of androgens in the mammalian testis. It is established that the luteinizing hormone (LH) produced by the anterior pituitary is required to maintain the structure and function of the Leydig cells in the postnatal testis. Until recent years, a role by the thyroid hormones on Leydig c...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2005-11-01 00:00:00
abstract：:Cognitive disorders in mankind are not uncommon. Apart from neurodegenerative diseases such as Alzheimer's (AD), various stresses also affect cognitive functions. Plants are known to be potential source of compounds that ameliorate several diseases including cognitive impairment. Here, we evaluated effect of aqueous e...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2016-02-01 00:00:00
abstract：:Nine halophilic archaea viz., Halobacterium salinarum, Halobacterium sp.1, Halobacterium sp.2, Halobaculum sp., Halococcus saccharolyticus, Halorubrum saccharovorum, Haloterrigena turkmenica, Halogeometricum sp. and Natrialba sp. isolated from marine salterns around Bhavnagar coast were screened for siderophore produc...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
abstract：:Mouse melanoma cells were treated with plumbagin, a naphthoquinone, from the plant Plumbago rosea at 0.5 microgram/ml (PI) for 60 min either alone or followed by 2 Gy gamma radiation (RT). Response to the different treatments was assessed by following the cell growth up to 5 days post treatment. PI alone produced a si...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:The effects of dopamine (DA) agonists and antagonists were investigated on indomethacin--and restraint stress (6 hr at RT)--induced gastric ulcer formation in rats. The DA-agonists, apomorphine and bromocryptine (both at 5 mg/kg) significantly attenuated the frequency and severity of gastric mucosal lesions in both ex...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:The permeability of the cornea to drugs is clinically important because it is the major factor determining the efficacy of topically applied ophthalmic preparations. With this perspective, the present article gives a brief update and overview of corneal structure and proposed mechanisms of permeation. Physiological, p...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:A study was conducted of the effect of intravaginal instillation of 3 nonsteroidal oran antifertility agents in pregnant rats. The 3 compounds used were 2-phenyl-3-rho-beta-pyrrolidinoethoxyphenyl-6-methox y benzofuran hydrochoride (DBF), 20 mg/kg; 2-phenyl-3-rho-beta-pyrrolidi noethoxyphenyl-naptha (2:1,b furan (NF...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1974-07-01 00:00:00
abstract：:Breast cancer susceptibility gene (BRCA1) is known to be responsible for hereditary breast and ovarian cancer. This gene is highly penetrant conferring a risk for 0.92 by the age of 70. Germline mutation in this gene leads to susceptibility to breast and ovarian cancer, with a genotype phenotype correlation. Frequency...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2001-05-01 00:00:00
abstract：:Preventive role of lipistat against doxorubicin induced myocardial toxicity in rats has been reported. Cardiotoxicity was produced by doxorubicin administration (15 mg/kg for 2 weeks). Lipistat (350 mg/kg, orally) was administered as pretreatment for 2 weeks and then for 2 weeks alternated with doxorubicin. The genera...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Effect of chloride and diamide on testicular and epididymal angiotensin converting enzyme (ACE) activity was investigated using Hip-His-Leu as substrate in sheep. The chloride ions functioned as ACE activators, however, there was no linear correlation between the two. The optimum chloride concentrations were 500 mM fo...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Treatment of full-thickness wounds with A. vera, on rats resulted in increased biosynthesis of collagen and its degradation. A corresponding increase in the urinary excretion of hydroxyproline was also observed. Elevated levels of lysyl oxidase also indicated increased crosslinking of newly synthesised collagen. The r...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:The frequency and types of acrocentric chromosome association were quantitatively analysed in a Down syndrome child with unusual karyotype, 46, XX, -14, -22, t dic (14p;22p), +21, 21S+. Father and 4 sibs were heterozygous carriers for t dic (14p;22p). The variant 21S+ was inherited from the mother. The occurrence of t...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:In denervation, there was loss of protein in gastrocnemius muscles and this loss of was more in prednisolone treated animals. There was significant change of protein loss between tenotomy and tenotomy with prednisolone treatment. The reduction of protein in denervation and denervation with prednisolone treatment were ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
abstract：:There was a significant increase in fucose (52%), total hexoses (16%) and hexosamine (56%) except sialic acid, which was reduced (77%) in the microvillus membrane of infants born to rat mothers made diabetic by injecting alloxan on day 3 of gestation. Expressed on the protein basis there were a significant increase in...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Germ cell death and their removal from the seminiferous epithelium are common in the affected testis in conditions of unilateral ischemia or cryptorchidism; the similarities and differences, however, have not been studied between these two conditions. The present study was designed to examine the severity of the effec...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:Considering the lipophilicity of aspirin (log P = -1.15), a significant contributor to its action mechanism, interaction of the drug with the whole lipids of goat blood have been investigated using phospholipid binding and lipid peroxidation phenomena as the parameters under investigation. The lipid content change alo...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:The effect of the administration of three different concentrations (2.25, 1.13 and 0.56 mg/100 g body weight) of dimethoate on the urinary excretion of total, non dialysable and free hydroxyproline were studied in female albino rats. Compared to controls, the urine contents were significantly lower in higher concentra...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:The role of magnesium (Mg2+) in the metabolism of Staphylococcus aureus was studied using a chemically defined medium. At least 0.01 mM Mg2+ was required for any growth of S. aureus. Increasing concentration of Mg2+ up to 1.6mM steadily increased the bacterial growth. Glucose utilisation and acid production were also ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Mouse B16LuF1 melanoma cells of lower metastatic potential to lung were treated in vitro with same concentration (50 microM) of gangliosides prepared from plasma of mice bearing lung metastasis of B16LuF5, B16LuF9 or B16LuF10 melanoma cell lines of increasing metastatic potential to lung (LuF1 < LuF5 < LuF9 < LuF10) a...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
abstract：:Convolvulus pluricaulis is an indigenous plant commonly mentioned in Ayurveda, an ancient system of Indian medicine, as a rasayana which is mainly advocated for use in rejuvenation therapy. The present study was conducted to evaluate the potential anti-ulcerogenic effect of juice of fresh whole plants of C. pluricauli...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:The monogenean ectoparasite, Dactylogyrus sp. is a major pathogen in freshwater aquaculture. The immune responses in parasitized fish were analyzed by quantitation of innate immune factors (natural agglutinin level, haemolysin titre, antiprotease, lysozyme and myeloperoxidase activities) in serum and immune-relevant g...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：:Therapeutic efficacy of EGTA (ethylene glycol bis (2-aminoethyl ether) tetraacetic acid) against cerium intoxicated mice was studied. Administration of cerium showed significant decrease in haemoglobin percentage, RBC counts and blood glucose level with an increase in the activity of serum transaminases and WBC counts...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：:Since aging is the most important risk factor for variety of diseases, the discovery of a wide range of chemical modulators of aging in model organisms encourages new strategies for targeting age associated diseases. Simple genetic manipulation leads to long-lived and healthy animals, so any compound which could have ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：:Intestinal uptake of lysine in rats progressively decreased with an increase in pH from 5.2 to 8.5, both in the presence and absence of Na+ ions. At pH 5.2 lysine uptake was 30-35% more than that at neutral pH. Na+ activated lysine uptake by 40-50% at pH 5.2 and it was increased to 110-120% at neutral pH. The observed...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
