Abstract：
:Biopharming for the production of recombinant pharmaceutical proteins in the mammary gland of transgenic animals is an attractive but laborious alternative compared to mammalian cell fermentation. The disadvantage of the lengthy process of genetically modifying an entire animal could be circumvented with somatic transduction of only the mammary epithelium with recombinant, replication-defective viruses. While other viral vectors offer very limited scope for this approach, vectors based on adeno-associated virus (AAV) appear to be ideal candidates because AAV is helper-dependent, does not induce a strong immune response and has no association with disease. Here, we sought to test the suitability of recombinant AAV (rAAV) for biopharming. Using reporter genes, we showed that injected rAAV efficiently transduced mouse mammary cells. When rAAV encoding human myelin basic protein (hMBP) was injected into the mammary glands of mice and rabbits, this resulted in the expression of readily detectable protein levels of up to 0.5 g/L in the milk. Furthermore we demonstrated that production of hMBP persisted over extended periods and that protein expression could be renewed in a subsequent lactation by re-injection of rAAV into a previously injected mouse gland.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wagner S,Thresher R,Bland R,Laible Gdoi
10.1038/srep15115subject
Has Abstractpub_date
2015-10-14 00:00:00pages
15115issn
2045-2322pii
srep15115journal_volume
5pub_type
杂志文章abstract：:The major human pathogen Streptococcus pneumoniae is a leading cause of disease and death worldwide. Pneumococcal biofilm formation within the nasopharynx leads to long-term colonization and persistence within the host. We have previously demonstrated that the capsular surface-associated pneumococcal serine rich repea...
journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章,meta分析
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pub_type： 杂志文章
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更新日期：2015-03-02 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-07-07 00:00:00
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pub_type： 杂志文章
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更新日期：2019-11-22 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2015-03-09 00:00:00
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pub_type： 杂志文章
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更新日期：2019-03-07 00:00:00
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pub_type： 杂志文章
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更新日期：2017-08-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08666
更新日期：2015-03-02 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章,meta分析,评审
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pub_type： 杂志文章,随机对照试验
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更新日期：2019-03-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-03-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08650
更新日期：2015-03-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37552
更新日期：2016-11-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14675
更新日期：2015-09-30 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53286-z
更新日期：2019-11-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-08-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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