当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > VEGF/Flk1 Mechanism is Involved in Roxarsone Promotion of Rat Endothelial Cell Growth and B16F10 Xenograft Tumor Angiogenesis.

VEGF/Flk1 Mechanism is Involved in Roxarsone Promotion of Rat Endothelial Cell Growth and B16F10 Xenograft Tumor Angiogenesis.

Abstract：

:The potential angiogenic effect of roxarsone, a feed additive widely used to promote animal growth worldwide, was demonstrated recently. We explored the mechanism of vascular endothelial growth factor (VEGF) and its receptor (VEGFR) in roxarsone promotion of rat vascular endothelial cells (ECs) and B16F10 mouse xenografts. ECs were treated with 0.1-50 μM roxarsone or with roxarsone plus 10 ng/mL VEGF, VEGFR1 (Flt1), or VEGFR2 (Flk1) antibodies for 12-48 h to examine their role in cell growth promotion. Small interfering RNA (siRNA) targeting Vegf, Flt1, and Flk1 were transfected in the ECs, and we measured the expression level, cell proliferation, migration, and tube formation ability. The siRNA targeting Vegf or Flk1 were injected intratumorally in the B16F10 xenografts of mice that received 25 mg/kg roxarsone orally. Cell viability and VEGF expression following roxarsone treatment were significantly higher than that of the control (P < 0.05), peaking following treatment with 1.0 μM roxarsone. Compared to roxarsone alone, the VEGF antibody decreased cell promotion by roxarsone (P < 0.05), and the Flk1 antibody greatly reduced cell viability compared to the Flt1 antibody (P < 0.01). Roxarsone and Flk1 antibody co-treatment increased supernatant VEGF significantly, while cellular VEGF was obviously decreased (P < 0.01), whereas there was no significant difference following Flt1 antibody blockade. The siRNA against Vegf or Flk1 significantly attenuated the roxarsone promotion effects on EC proliferation, migration, and tube-like formation (P < 0.01), whereas the siRNA against Flt1 effected no obvious differences. Furthermore, the RNA interference significantly weakened the roxarsone-induced increase in xenograft weight and volume, and VEGF and Flk1 expression. Roxarsone promotion of rat EC growth, migration, and tube-like formation in vitro and of B16F10 mouse xenograft model tumor growth and angiogenesis involves a VEGF/Flk1 mechanism.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Chen S,Xu J,Wei Q,Zhao Z,Chen X,Cui H,Zhang Y

doi

10.1038/s41598-019-53870-3

subject

Has Abstract

pub_date

2019-11-22 00:00:00

pages

17417

issue

1

issn

2045-2322

pii

10.1038/s41598-019-53870-3

journal_volume

9

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Preclinical dosimetry models and the prediction of clinical doses of novel positron emission tomography radiotracers.

    abstract：:Dosimetry models using preclinical positron emission tomography (PET) data are commonly employed to predict the clinical radiological safety of novel radiotracers. However, unbiased clinical safety profiling remains difficult during the translational exercise from preclinical research to first-in-human studies for nov...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-72830-w

    authors： Garrow AA,Andrews JPM,Gonzalez ZN,Corral CA,Portal C,Morgan TEF,Walton T,Wilson I,Newby DE,Lucatelli C,Tavares AAS

    更新日期：2020-09-29 00:00:00

  • Fine-tuning of synaptic upscaling at excitatory synapses by endocannabinoid signaling is mediated via the CB1 receptor.

    abstract：:The endocannabinoid 2-arachidonoylglycerol (2-AG) functions as a retrograde signaling molecule mediating synaptic transmission and plasticity at both inhibitory and excitatory synapses. However, little is known about whether 2-AG signaling is involved in homeostatic regulation of miniature synaptic events at excitator...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep16257

    authors： Song Y,Zhang J,Chen C

    更新日期：2015-11-06 00:00:00

  • Comparison of biological H2S removal characteristics between a composite packing material with and without functional microorganisms.

    abstract：:The performances of two identical biofilters, filled with a new composite packing material (named CM-5) embedded with functional microorganisms or sterilized CM-5 without microorganisms, were investigated for H2S treatment. Running parameters in terms of microbial counts, pressure drops, and inlet and outlet H2S conce...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep42241

    authors： Zhu R,Li S,Bao X,Dumont É

    更新日期：2017-02-13 00:00:00

  • Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor.

    abstract：:In this study, we identified a CD105+CD90.1-CD133-CD55- (CD133-CD55-) population in the fetal skeletal element that can generate bone and bone marrow. Besides osteoblasts and chondrocytes, the CD133-CD55- common progenitors can give rise to marrow reticular stromal cells and perivascular mesenchymal progenitors sugges...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38632

    authors： Weng L,Hu X,Kumar B,Garcia M,Todorov I,Jung X,Marcucci G,Forman SJ,Chen CC

    更新日期：2016-12-08 00:00:00

  • Tailoring self-organized nanostructured morphologies in kilometer-long polymer fiber.

    abstract：:While nanowires and nanospheres have been utilized in the design of a diverse array of nanoscale devices, recent schemes frequently require nanoscale architectures of higher complexity. However, conventional techniques are largely unsatisfactory for the production of more intricate nanoscale shapes and patterns, and e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep04864

    authors： Khudiyev T,Tobail O,Bayindir M

    更新日期：2014-05-06 00:00:00

  • RNA sequencing reveals a transcriptomic portrait of human mesenchymal stem cells from bone marrow, adipose tissue, and palatine tonsils.

    abstract：:Human mesenchymal stem cells (MSCs) are adult multipotent cells that have plasticity and inhabit the stroma of diverse tissues. The potential utility of MSCs has been heavily investigated in the fields of regenerative medicine and cell therapy. However, MSCs represent diverse populations that may depend on the tissue ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-16788-2

    authors： Cho KA,Park M,Kim YH,Woo SY,Ryu KH

    更新日期：2017-12-07 00:00:00

  • Lobar microbleeds are associated with cognitive impairment in patients with lacunar infarction.

    abstract：:Associations between cognitive decline and cerebral microbleeds (CMBs) have received increasing attention. An association between CMB distribution (deep or lobar) and cognitive decline has been reported, but these findings are controversial. We investigated the association between magnetic resonance imaging (MRI) find...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-73404-6

    authors： Nakamori M,Hosomi N,Tachiyama K,Kamimura T,Matsushima H,Hayashi Y,Imamura E,Wakabayashi S,Maruyama H

    更新日期：2020-10-02 00:00:00

  • Efficacy of alprostadil for preventing of contrast-induced nephropathy: A meta-analysis.

    abstract：:Contrast-induced nephropathy (CIN) has become the third-leading cause of hospital-acquired acute renal injury. Although alprostadil has been proposed as an effective preventative measure, this conclusion remains inconsistent. Thus, we performed a meta-analysis of the published studies on this topic to evaluate the pre...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析

    doi：10.1038/s41598-017-01160-1

    authors： Zhang JZ,Kang XJ,Gao Y,Zheng YY,Wu TT,Li L,Liu F,Yang YN,Li XM,Ma YT,Xie X

    更新日期：2017-04-21 00:00:00

  • Immobilization of Polyoxometalate in the Metal-Organic Framework rht-MOF-1: Towards a Highly Effective Heterogeneous Catalyst and Dye Scavenger.

    abstract：:A series of three remarkable complexes [PMo12O40]@[Cu6O(TZI)3(H2O)9]4·OH·31H2O (H3TZI = 5-tetrazolylisophthalic acid; denoted as HLJU-1, HLJU = Heilongjiang University), [SiMo12O40]@[Cu6O(TZI)3(H2O)9]4·32H2O (denoted as HLJU-2), and [PW12O40]@[Cu6O(TZI)3(H2O)6]4·OH·31H2O (denoted as HLJU-3) have been isolated by using...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep25595

    authors： Sun JW,Yan PF,An GH,Sha JQ,Li GM,Yang GY

    更新日期：2016-05-09 00:00:00

  • Natural history footage provides new reef fish biodiversity information for a pristine but rarely visited archipelago.

    abstract：:There remain parts of our planet that are seldom visited by humans, let alone scientists. In such locations, crowd-sourced or citizen scientist data can be critical in describing biodiversity and detecting change. Rangitāhua, the Kermadec Islands, are 750 km from the nearest human-habitation. Although our knowledge of...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-60136-w

    authors： Liggins L,Sweatman JA,Trnski T,Duffy CAJ,Eddy TD,Aguirre JD

    更新日期：2020-02-21 00:00:00

  • Fluorescence laser microdissection reveals a distinct pattern of gene activation in the mouse hippocampal region.

    abstract：:A histoanatomical context is imperative in an analysis of gene expression in a cell in a tissue to elucidate physiological function of the cell. In this study, we made technical advances in fluorescence laser microdissection (LMD) in combination with the absolute quantification of small amounts of mRNAs from a region ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep00783

    authors： Yoshioka W,Endo N,Kurashige A,Haijima A,Endo T,Shibata T,Nishiyama R,Kakeyama M,Tohyama C

    更新日期：2012-01-01 00:00:00

  • Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation.

    abstract：:Magnesium has been shown to effectively prevent vascular calcification associated with chronic kidney disease. Magnesium has been hypothesized to prevent the upregulation of osteoblastic genes that potentially drives calcification. However, extracellular effects of magnesium on hydroxyapatite formation are largely neg...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-20241-3

    authors： Ter Braake AD,Tinnemans PT,Shanahan CM,Hoenderop JGJ,de Baaij JHF

    更新日期：2018-02-01 00:00:00

  • Pre-operative sera interleukin-6 in the diagnosis of high-grade serous ovarian cancer.

    abstract：:Pre-operative discrimination of malignant masses is crucial for accurate diagnosis and prompt referral to a gynae oncology centre for optimal surgical intervention. HGSOC progression is correlated with local and systemic inflammation. We hypothesised that inclusion of inflammatory biomarkers in sera may improve diagno...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-59009-z

    authors： Kampan NC,Madondo MT,Reynolds J,Hallo J,McNally OM,Jobling TW,Stephens AN,Quinn MA,Plebanski M

    更新日期：2020-02-10 00:00:00

  • Serum neurofilament light chain predicts long term clinical outcomes in multiple sclerosis.

    abstract：:Serum neurofilament light chain (NfL) is emerging as an important biomarker in multiple sclerosis (MS). Our objective was to evaluate the prognostic value of serum NfL levels obtained close to the time of MS onset with long-term clinical outcomes. In this prospective cohort study, we identified patients with serum col...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-67504-6

    authors： Thebault S,Abdoli M,Fereshtehnejad SM,Tessier D,Tabard-Cossa V,Freedman MS

    更新日期：2020-06-25 00:00:00

  • Optical absorption in array of Ge/Al-shell nanoparticles in an Alumina matrix.

    abstract：:The absorption spectra in array of Ge, Al and Ge/Al-shell nanoparticles immersed in alumina (Al2O3) matrix is calculated in framework of ab initio macroscopic dielectric model. It is demonstrated that absorption is strongly enhanced when germanium nanospheres are encapsulated by Al-shell. Two absorption peaks, appeari...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56673-8

    authors： Despoja V,Basioli L,Parramon JS,Mičetić M

    更新日期：2020-01-09 00:00:00

  • Cholestasis induced liver pathology results in dysfunctional immune responses after arenavirus infection.

    abstract：:Immune responses are critical for defense against pathogens. However, prolonged viral infection can result in defective T cell immunity, leading to chronic viral infection. We studied immune activation in response to arenavirus infection during cholestasis using bile duct ligation (BDL). We monitored T cell responses,...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-30627-y

    authors： Lang E,Pozdeev VI,Shinde PV,Xu HC,Sundaram B,Zhuang Y,Poschmann G,Huang J,Stühler K,Pandyra AA,Keitel V,Häussinger D,Lang KS,Lang PA

    更新日期：2018-08-15 00:00:00

  • A broadband terahertz ultrathin multi-focus lens.

    abstract：:Ultrathin transmission metasurface devices are designed on the basis of the Yang-Gu amplitude-phase retrieval algorithm for focusing the terahertz (THz) radiation into four or nine spots with focal spacing of 2 or 3 mm at a frequency of 0.8 THz. The focal properties are experimentally investigated in detail, and the r...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28800

    authors： He J,Ye J,Wang X,Kan Q,Zhang Y

    更新日期：2016-06-27 00:00:00

  • Molecular and Histopathological Study on the Ameliorative Impacts of Petroselinum Crispum and Apium Graveolens against Experimental Hyperuricemia.

    abstract：:Hyperuricemia is an abnormal metabolic condition characterized by an increase in uric acid levels in the blood. It is the cause of gout, manifested by inflammatory arthritis, pain and disability. This study examined the possible ameliorative impacts of parsley (PAR) and celery (CEL) as hypouricemic agents at biochemic...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66205-4

    authors： Soliman MM,Nassan MA,Aldhahrani A,Althobaiti F,Mohamed WA

    更新日期：2020-06-11 00:00:00

  • Predictors and their prognostic value for no ROSC and mortality after a non-cardiac surgery intraoperative cardiac arrest: a retrospective cohort study.

    abstract：:Data on predictors of intraoperative cardiac arrest (ICA) outcomes are scarce in the literature. This study analysed predictors of poor outcome and their prognostic value after an ICA. Clinical and laboratory data before and 24 hours (h) after ICA were analysed as predictors for no return of spontaneous circulation (R...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-51557-3

    authors： Vane MF,Carmona MJC,Pereira SM,Kern KB,Timerman S,Perez G,Vane LA,Otsuki DA,Auler JOC Jr

    更新日期：2019-10-18 00:00:00

  • Age-dependent motor dysfunction due to neuron-specific disruption of stress-activated protein kinase MKK7.

    abstract：:c-Jun N-terminal kinase (JNK) is a member of the mitogen-activated protein kinase family and controls various physiological processes including apoptosis. A specific upstream activator of JNKs is the mitogen-activated protein kinase kinase 7 (MKK7). It has been reported that MKK7-JNK signaling plays an important regul...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07845-x

    authors： Yamasaki T,Deki-Arima N,Kaneko A,Miyamura N,Iwatsuki M,Matsuoka M,Fujimori-Tonou N,Okamoto-Uchida Y,Hirayama J,Marth JD,Yamanashi Y,Kawasaki H,Yamanaka K,Penninger JM,Shibata S,Nishina H

    更新日期：2017-08-04 00:00:00

  • Assessment of different techniques for 3D superimposition of serial digital maxillary dental casts on palatal structures.

    abstract：:Serial 3-dimensional dental model superimposition provides a risk-free, detailed evaluation of morphological alterations on a patient's mouth. Here, we evaluated accuracy and precision of five palatal areas, used for superimposition of maxillary 3D digital dental casts. Sixteen pre- and post-orthodontic treatment dent...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-06013-5

    authors： Vasilakos G,Schilling R,Halazonetis D,Gkantidis N

    更新日期：2017-07-19 00:00:00

  • Kinetic 15N-isotope effects on algal growth.

    abstract：:Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with current theoretical and e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep44181

    authors： Andriukonis E,Gorokhova E

    更新日期：2017-03-10 00:00:00

  • Backward spoof surface wave in plasmonic metamaterial of ultrathin metallic structure.

    abstract：:Backward wave with anti-parallel phase and group velocities is one of the basic properties associated with negative refraction and sub-diffraction image that have attracted considerable interest in the context of photonic metamaterials. It has been predicted theoretically that some plasmonic structures can also suppor...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20448

    authors： Liu X,Feng Y,Zhu B,Zhao J,Jiang T

    更新日期：2016-02-04 00:00:00

  • HPV16 early gene E5 specifically reduces miRNA-196a in cervical cancer cells.

    abstract：:High-risk human papillomavirus (HPV) type 16, which is responsible for greater than 50% of cervical cancer cases, is the most prevalent and lethal HPV type. However, the molecular mechanisms of cervical carcinogenesis remain elusive, particularly the early steps of HPV infection that may transform normal cervical epit...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07653

    authors： Liu C,Lin J,Li L,Zhang Y,Chen W,Cao Z,Zuo H,Chen C,Kee K

    更新日期：2015-01-07 00:00:00

  • Phylogenetic relationships among tribes of the green lacewing subfamily Chrysopinae recovered based on mitochondrial phylogenomics.

    abstract：:Chrysopidae (green lacewings) is the second largest family in Neuroptera, and it includes medium-size lacewings largely recognized by the presence of golden-colored eyes, bright green bodies and delicate wings with dense venation patterns. The subfamily Chrysopinae includes 97% of the species diversity in the family a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07431-1

    authors： Jiang Y,Garzón-Orduña IJ,Winterton SL,Yang F,Liu X

    更新日期：2017-08-03 00:00:00

  • Author Correction: Relative posture between head and finger determines perceived tactile direction of motion.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-65967-1

    authors： Chen YP,Yeh CI,Lee TC,Huang JJ,Pei YC

    更新日期：2020-06-04 00:00:00

  • The rapid "teabag" method for high-end purification of membrane proteins.

    abstract：:Overproduction and purification of membrane proteins are generally challenging and time-consuming procedures due to low expression levels, misfolding, and low stability once extracted from the membrane. Reducing processing steps and shortening the timespan for purification represent attractive approaches to overcome s...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-73285-9

    authors： Hering J,Missel JW,Zhang L,Gunnarsson A,Castaldo M,Pedersen PA,Ek M,Gourdon P,Snijder HJ

    更新日期：2020-09-30 00:00:00

  • Transcript profile analysis reveals important roles of jasmonic acid signalling pathway in the response of sweet potato to salt stress.

    abstract：:Sweet potato is an important food and bio-energy crop, and investigating the mechanisms underlying salt tolerance will provide information for salt-tolerant breeding of this crop. Here, the root transcriptomes of the salt-sensitive variety Lizixiang and the salt-tolerant line ND98 were compared to identify the genes a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep40819

    authors： Zhang H,Zhang Q,Zhai H,Li Y,Wang X,Liu Q,He S

    更新日期：2017-01-13 00:00:00

  • Deciphering the mode of action of cell wall-inhibiting antibiotics using metabolic labeling of growing peptidoglycan in Streptococcus pyogenes.

    abstract：:Because of the scanty pipeline of antibiotics newly obtained from nature, chemical modification of established drugs is one of the major streams of current antibacterial research. Intuitive and easy-to-use assays are critical for identifying drug candidates with novel modes of action. In this study, we demonstrated th...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-01267-5

    authors： Sugimoto A,Maeda A,Itto K,Arimoto H

    更新日期：2017-04-25 00:00:00

  • Differential Responses of Plasma Adropin Concentrations To Dietary Glucose or Fructose Consumption In Humans.

    abstract：:Adropin is a peptide hormone encoded by the Energy Homeostasis Associated (ENHO) gene whose physiological role in humans remains incompletely defined. Here we investigated the impact of dietary interventions that affect systemic glucose and lipid metabolism on plasma adropin concentrations in humans. Consumption of gl...

    journal_title：Scientific reports

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/srep14691

    authors： Butler AA,St-Onge MP,Siebert EA,Medici V,Stanhope KL,Havel PJ

    更新日期：2015-10-05 00:00:00