Abstract：
:Human activating signal cointegrator homology (ASCH) domain-containing proteins are widespread and diverse but, at present, the vast majority of those proteins have no function assigned to them. This study demonstrates that the 103-amino acid Escherichia coli protein YqfB, previously identified as hypothetical, is a unique ASCH domain-containing amidohydrolase responsible for the catabolism of N4-acetylcytidine (ac4C). YqfB has several interesting and unique features: i) it is the smallest monomeric amidohydrolase described to date, ii) it is active towards structurally different N4-acylated cytosines/cytidines, and iii) it has a high specificity for these substrates (kcat/Km up to 2.8 × 106 M-1 s-1). Moreover, our results suggest that YqfB contains a unique Thr-Lys-Glu catalytic triad, and Arg acting as an oxyanion hole. The mutant lacking the yqfB gene retains the ability to grow, albeit poorly, on N4-acetylcytosine as a source of uracil, suggesting that an alternative route for the utilization of this compound exists in E. coli. Overall, YqfB ability to hydrolyse various N4-acylated cytosines and cytidines not only sheds light on the long-standing mystery of how ac4C is catabolized in bacteria, but also expands our knowledge of the structural diversity within the active sites of amidohydrolases.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Stanislauskienė R,Laurynėnas A,Rutkienė R,Aučynaitė A,Tauraitė D,Meškienė R,Urbelienė N,Kaupinis A,Valius M,Kaliniene L,Meškys Rdoi
10.1038/s41598-020-57664-wsubject
Has Abstractpub_date
2020-01-21 00:00:00pages
788issue
1issn
2045-2322pii
10.1038/s41598-020-57664-wjournal_volume
10pub_type
杂志文章abstract：:Gold nanoparticles (GNP) have been intensively investigated for applications in cancer imaging and therapy. Most imaging studies focused on microscopic imaging. Their potential as optical imaging probes for whole body small animal imaging has rarely been explored. Taking advantage of their surface plasmon resonance (S...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27624-6
更新日期：2018-06-19 00:00:00
abstract：:Neurite growth is controlled by a complex molecular signaling network that regulates filamentous actin (F-actin) dynamics at the growth cone. The evolutionarily conserved ezrin, radixin, and moesin family of proteins tether F-actin to the cell membrane when phosphorylated at a conserved threonine residue and modulate ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20755-w
更新日期：2018-02-07 00:00:00
abstract：:Carbon coated TiO2 (TiO2@C) is fabricated by a convenient and green one-pot solvothermal method, in which ethylene glycol serve as both the reaction medium and carbon source without the addition of any other carbon additives. During the solvothermal process, ethylene glycol polymerize and coordinate with Ti4+ to form ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43895
更新日期：2017-03-03 00:00:00
abstract：:Climatic characteristics of Hengduan Mountains region were diverse, and Eothenomys miletus was a native species throughout this region. To investigate adaptive strategies of E. miletus to environmental factors in different locations in this region, five locations were selected, including Deqin (DQ), Xianggelila (XGLL)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51493-2
更新日期：2019-10-17 00:00:00
abstract：:We performed an integrated analysis of the transcriptome and metabolome from purple (Pr) and normal cultivated varieties (CK) of Dendrobium officinale to gain insights into the regulatory networks associated with phenylpropanoid metabolism and to identify the key regulatory genes of pigmentation. Metabolite and transc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74789-0
更新日期：2020-10-19 00:00:00
abstract：:Despite recent advances, the myocardial microstructure remains imperfectly understood. In particular, bundles of cardiomyocytes have been observed but their three-dimensional organisation remains debated and the associated mechanical consequences unknown. One of the major challenges remains to perform multiscale obser...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76820-w
更新日期：2020-11-25 00:00:00
abstract：:Desertification due to climate change and increasing drought periods is a worldwide problem for both ecology and economy. Our ability to understand how vegetation manages to survive and propagate through arid and semiarid ecosystems may be useful in the development of future strategies to prevent desertification, pres...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33703
更新日期：2016-09-21 00:00:00
abstract：:Antibiogram profile of 1590 clinical bacterial isolates based on thirteen different antimicrobial compounds showed that 1.6% of the bacterial isolates are multidrug resistant. Distribution pattern based on 16S rRNA sequence analysis showed that Pseudomonas aeruginosa constituted the largest group (83.6%) followed by B...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23156
更新日期：2016-03-18 00:00:00
abstract：:Understanding ethanol-induced stresses and responses in biofuel-producing bacteria at systems level has significant implications in engineering more efficient biofuel producers. We present a computational study of transcriptomic and genomic data of both ethanol-stressed and ethanol-adapted E. coli cells with computati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44150
更新日期：2017-03-16 00:00:00
abstract：:Luminance plays a modulating role in the processes of several visual tasks, which in turn provides significant information for the understanding of visual processing. Here, using a binocular phase combination paradigm, we studied the effect of unilateral changes in mean luminance on binocular combination in both norma...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02012
更新日期：2013-01-01 00:00:00
abstract：:Mumps incidence in mainland China remains at a high level. Genotype F has been the predominant genotype of mumps virus (MuV) in the last 20 years in mainland China. To better understand the genetic characteristics of MuV in China, the sequences of the Small Hydrophobic (SH), Hemagglutinin-Neuraminidase (HN) and Fusion...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17474-z
更新日期：2017-12-07 00:00:00
abstract：:Millions of people are blind worldwide. Sensory substitution (SS) devices (e.g., vOICe) can assist the blind by encoding a video stream into a sound pattern, recruiting visual brain areas for auditory analysis via crossmodal interactions and plasticity. SS devices often require extensive training to attain limited fun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15628
更新日期：2015-10-22 00:00:00
abstract：:Rapid eye movement (REM) sleep is considered to preferentially reprocess emotionally arousing memories. We tested this hypothesis by cueing emotional vs. neutral memories during REM and NREM sleep and wakefulness by presenting associated verbal memory cues after learning. Here we show that cueing during NREM sleep sig...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39229
更新日期：2016-12-16 00:00:00
abstract：:Pesticides are commonly used in food crop production systems to control crop pests and diseases and ensure maximum yield with high market value. However, the accumulation of these chemical inputs in crop fields increases risks to biodiversity and human health. In addition, people are increasingly seeking foods in whic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46688-6
更新日期：2019-07-15 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-24993-w
更新日期：2018-05-04 00:00:00
abstract：:Exploration of macroscopic quantum entanglement is of great interest in both fundamental science and practical application. We investigate a hybrid quantum system that consists of two nitrogen-vacancy centers ensembles (NVE) coupled to a superconducting coplanar waveguide resonator (CPWR). The collective magnetic coup...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21775
更新日期：2016-02-23 00:00:00
abstract：:Mid-infrared quantum cascade lasers operating under external optical feedback and external periodic bias forcing are shown to exhibit a deterministic chaotic pattern composed of frequencies which are linked to the one of the forcing. Results also show that both the amplitude and the frequency of the forcing play a key...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40861-7
更新日期：2019-03-14 00:00:00
abstract：:The expression level of folate receptor alpha (FRα) is located highly rate in ovarian cancer though it is remained absent in normal tissues. This highly tumor restricted expression profile makes FRα a promising target for tumor therapy and diagnosis. In this research we report a FRα binding peptide C7(Met-His-Thr-Ala-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26683-z
更新日期：2018-05-30 00:00:00
abstract：:Helicobacter pylori is presumed to infect gastric tissue via the oral cavity in childhood, whereas risk factors for H. pylori infection in the oral cavity are unknown. In this study, we analysed the effects of Streptococcus mutans, a major cariogenic bacterial species, on H. pylori colonisation in the oral cavity, as ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69368-2
更新日期：2020-07-27 00:00:00
abstract：:Upconversion nanoparticles (UCNPs) are utilized extensively for biomedical imaging, sensing, and therapeutic applications, yet the molecular weight of UCNPs has not previously been reported. Herein, we present a theory based upon the crystal structure of UCNPs to estimate the molecular weight of UCNPs: enabling insigh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19415-w
更新日期：2018-01-18 00:00:00
abstract：:Lung cancer is the foremost cause of cancer related deaths in the U.S. It is a heterogeneous disease composed of genetically and phenotypically distinct tumor cells surrounded by heterotypic cells and extracellular matrix dynamically interacting with the tumor cells. Research in lung cancer is often restricted to pati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41301-2
更新日期：2019-03-20 00:00:00
abstract：:In this study, we sequenced the complete mitochondrial genome of Eligma narcissus and compared it with 18 other lepidopteran species. The mitochondrial genome (mitogenome) was a circular molecule of 15,376 bp containing 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26387
更新日期：2016-05-25 00:00:00
abstract：:The activation and regulation of coagulation Factor XIII (FXIII) protein has been the subject of active research for the past three decades. Although discrete evidence exists on various aspects of FXIII activation and regulation a combinatorial structure/functional view in this regard is lacking. In this study, we pre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30105
更新日期：2016-07-25 00:00:00
abstract：:Epididymal Cysteine Rich Secretory Proteins 1 and 4 (CRISP1 and CRISP4) associate with sperm during maturation and play different roles in fertilization. However, males lacking each of these molecules individually are fertile, suggesting compensatory mechanisms between these homologous proteins. Based on this, in the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35719-3
更新日期：2018-12-03 00:00:00
abstract：:Gastric cancer is one of the most aggressive cancers and is the second leading cause of cancer death worldwide. Approximately 40% of global gastric cancer cases occur in China, with peritoneal metastasis being the prevalent form of recurrence and metastasis in advanced disease. Currently, there are limited clinical ap...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13750
更新日期：2015-09-02 00:00:00
abstract：:Detection of sedimentary cycles is difficult in fine-grained or homogenous sediments but is a prerequisite for the interpretation of depositional environments. Here we use a new autocorrelation analysis to detect cycles in a homogenous sediment core, E602, from the northern shelf of the South China Sea. Autocorrelatio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01653
更新日期：2013-01-01 00:00:00
abstract：:Cover cropping is proposed to enhance soil microbial diversity and activity, with cover crop type affecting microbial groups in different ways. We compared fungal community compositions of bulk soils differing by cover crop treatment, season, and edaphic properties in the third year of an organic, conventionally tille...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63173-7
更新日期：2020-04-10 00:00:00
abstract：:Disseminated tumor cells (DTCs) in the bone marrow (BM) and circulating tumor cells (CTCs) in blood of breast cancer patients (pts) are known to correlate with worse outcome. Here we demonstrate a different prognostic value of DTCs and CTCs and explain these findings by early clodronate intake. CTCs (n = 376 pts) were...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26355
更新日期：2016-05-23 00:00:00
abstract：:Accumulating evidence across species and memory domains shows that when an existing memory is reactivated, it becomes susceptible to modifications. However, the potential role of reward signals in these mechanisms underlying human memory dynamics is unknown. Leaning on a wealth of findings on the role of reward in rei...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28270
更新日期：2016-06-16 00:00:00
abstract：:Bacterial vaginosis (BV) causes genital inflammation and increased HIV acquisition risk. The standard-of-care for BV, antibiotic therapy, is associated with high recurrence rates. Probiotics may improve treatment outcomes, although substantial heterogeneity in efficacy has been observed during clinical trials. To eval...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-018-38253-4
更新日期：2019-02-13 00:00:00
