Abstract:
INTRODUCTION:Enterococcus faecalis is a frequently isolated microorganism in persistent periapical lesion or secondary infection. However, no evidence has demonstrated that E. faecalis induced inflammation directly in the apical area. This study aimed to explore the mechanism of the inflammatory responses of human periodontal ligament fibroblasts (PDLs) to E. faecalis. METHODS:PDLs were stimulated with heat-killed E. faecalis (HKEF) or lipoteichoic acid from E. faecalis (LTA) with or without silencing of tumor necrosis factor receptor-associated factor 6 (TRAF6). The expressions of toll-like receptor 2/4, nucleotide-binding oligomerization domain 1/2, and TRAF6 were detected by using quantitative real-time polymerase chain reaction and Western blot. The secretions of proinflammatory cytokines, including interleukin-1β, interleukin-6, interleukin-8, and tumor necrosis factor-α, were determined in the cell supernatants with enzyme-linked immunosorbent assay. RESULTS:Both HKEF and LTA stimulated the expression of toll-like receptor 2 and TRAF6 in a time-dependent manner. The secretions of proinflammatory cytokines were also increased. After silencing TRAF6, the upregulations of proinflammatory cytokines induced by HKEF or LTA were attenuated. CONCLUSIONS:TRAF6 plays a pivotal role in inflammation induced by E. faecalis or its LTA in PDLs.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Zhang L,Wang T,Lu Y,Zheng Q,Gao Y,Zhou X,Huang Ddoi
10.1016/j.joen.2015.08.028subject
Has Abstractpub_date
2015-12-01 00:00:00pages
1997-2001issue
12eissn
0099-2399issn
1878-3554pii
S0099-2399(15)00793-1journal_volume
41pub_type
杂志文章abstract::The purpose of this study was to compare the reinforcement and strengthening ability of Resilon, gutta-percha, and a self-curing composite resin (Bisfil 2B) in endodontically treated roots of immature teeth. Sixty single rooted teeth were divided into five groups of 12 teeth each. Teeth in all groups except the negati...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.08.001
更新日期:2006-04-01 00:00:00
abstract::The purpose of this study was to evaluate the effect of occlusal load on the integrity of root canal obturation. Maxillary anterior teeth were cleaned, shaped, and obturated with either gutta-percha (GP)/AH Plus or Resilon/Epiphany. Afterwards, teeth were placed under 100 N force at 2 Hz for 22 hours in a 37 degrees C...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.01.022
更新日期:2008-04-01 00:00:00
abstract:INTRODUCTION:Electrically heated pluggers are the most commonly used instruments during warm obturation techniques. This study aimed to evaluate the effect of sterilization and operating temperature settings on the heat generation of pluggers of various taper sizes. METHODS:Fifty pluggers were sterilized at 132°C for ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.09.008
更新日期:2019-12-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to evaluate the effects of different curing units and 2 luting cements on the push-out bond strength of a translucent fiber post. METHODS:Thirty maxillary incisor roots were endodontically treated. Post spaces were prepared, and the smear layers were removed. Posts (FRC Postec Pl...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.04.026
更新日期:2010-09-01 00:00:00
abstract::This study sought to evaluate the influence of apical enlargement on the sealing ability of vertical compaction. Sixty anterior teeth were used. Half of these teeth were prepared to a size 25 file and the other half to a size 40 file. Then vertical compaction was performed. Apical microleakage was determined using pre...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)80091-9
更新日期:1994-07-01 00:00:00
abstract::The water sorption and solubility characteristics of three contemporary methacrylate resin-based endodontic sealers, EndoREZ, Epiphany, and InnoEndo, were compared with those obtained from Kerr EWT, Ketac-Endo (positive control), GuttaFlow, and AH Plus (both negative controls). Ten disks of each material were dehydrat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.03.021
更新日期:2007-08-01 00:00:00
abstract::Inflammation of pulp tissue appears as a consequence of caries progression. Its main characteristic is inflamed infiltrate whose cells contain lymphocytes. CD44 is a widely expressed adhesion molecule present in several body cells such as leukocytes and parenchymatous cells, including endothelial cells, epithelial cel...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.05.022
更新日期:2007-10-01 00:00:00
abstract::Porphyromonas endodontalis (formerly Bacteroides endodontalis) is a black-pigmented anaerobic Gram-negative rod which is associated with endodontal infections. It has been isolated from infected dental root canals and submucous abscesses of endodontal origin. The presence of P. endodontalis in infected dental root can...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80843-5
更新日期:1992-09-01 00:00:00
abstract::Osteoradionecrosis (ORN) of the jaw is considered the most severe long-term adverse effect that can occur in radiation therapy patients. Teeth extractions and dental diseases are the main risk factors for ORN in irradiated patients. The aim of this case series was to evaluate the outcome of primary root canal treatmen...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.12.005
更新日期:2020-04-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to compare the accuracy in vivo of 2 electronic apex locators (EALs), the Raypex 5 and the Mini Apex Locator. METHODS:The working length (WL) was determined electronically for 40 human root canals by using a K-file and 1 of the 2 EALs. The files were fixed at the WL, and the ...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2011.06.021
更新日期:2011-10-01 00:00:00
abstract:INTRODUCTION:An ideal orthograde or retrograde filling material should seal the pathways of communication between the root canal system and its surrounding tissues. It should also be nontoxic, noncarcinogenic, nongenotoxic, biocompatible, insoluble in tissue fluids, and dimensionally stable. Mineral trioxide aggregate ...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2009.09.006
更新日期:2010-01-01 00:00:00
abstract::The putative hazardous nature of chloroform and xylene implies that safer substitutes should be considered. Standard cylindrical gutta-percha samples were immersed in Hemo-De, a xylene substitute, for 60 s at 37 degrees C. Weight loss of a sample, after drying, was used as a measure of its solubility. Master and acces...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200007000-00001
更新日期:2000-07-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the antimicrobial effects of root canal sealers on Enterococcus faecalis biofilms in dentinal tubules by using a novel dentin infection model. METHODS:Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart inf...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.10.042
更新日期:2014-04-01 00:00:00
abstract::A molar-incisor malformation (MIM) is a newly reported type of dental anomaly that involves a characteristic root malformation in permanent first molars and deciduous second molars and a crown defect in permanent central incisors. This case report describes a nonsurgical root canal treatment of a MIM-affected molar by...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.11.004
更新日期:2016-04-01 00:00:00
abstract:INTRODUCTION:The exact mechanisms of periapical bone resorption have not been fully elucidated. This study aimed to analyze the expression of Notch signaling molecules (Notch2, Jagged1, and Hey1) and proinflammatory cytokines (tumor necrosis factor alpha [TNF-α], interleukin [IL]-1β, and IL-6) in human apical periodont...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.10.015
更新日期:2019-02-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate and compare the volume of removed dentin, transportation, and centering ability of ProTaper Next (PTN) system with and without glide path preparation by using cone-beam computed tomography (CBCT) imaging. METHODS:Sixty mesiobuccal canals of mandibular first molars...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.09.001
更新日期:2014-12-01 00:00:00
abstract:INTRODUCTION:This study examined the effect of a new bioactive dentin substitute material (Biodentine) on the viability of human gingival fibroblasts. METHODS:Biodentine, White ProRoot mineral trioxide aggregate (MTA), and glass ionomer cement were evaluated. Human gingival fibroblasts were incubated for 1, 3, and 7 d...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.11.026
更新日期:2013-04-01 00:00:00
abstract::Amalgam, IRM, and a mineral trioxide aggregate were tested for repair of experimentally created root perforations. Fifty sound, extracted mandibular and maxillary molars were used in this study. A perforation was created on the mesial root surface at about a 45-degree angle to the long axis of each tooth. The tooth wa...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81282-3
更新日期:1993-11-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to examine whether Enterococcus faecalis isolates from endodontic patients (from saliva and from a root canal) are able to prevail against salivary bacteria when grown in coculture in a biofilm reactor. METHODS:Saliva that was tested to be free of E. faecalis was used as the inoc...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.04.013
更新日期:2009-07-01 00:00:00
abstract:INTRODUCTION:Dental pulp has limited capability to regenerate, which happens in the early stage of pulpitis. An ambiguous relationship exists; inflammation may impair or support pulp regeneration. Epigenetics, which is involved in cell proliferation and inflammation, could regulate human dental pulp cell (HDPCs) regene...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.01.031
更新日期:2014-08-01 00:00:00
abstract::The purpose of this study was to evaluate the effect of different irrigating solutions on smear layer removal and dentinal tubule opening on root canal surfaces after post space preparation and to study whether additional ultrasonic irrigation has any effect on smear layer removal. Forty-eight anterior teeth were trea...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.01.006
更新日期:2009-04-01 00:00:00
abstract::The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 da...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80460-7
更新日期:1993-06-01 00:00:00
abstract:INTRODUCTION:The authors conducted a prospective, randomized, single-blind study to determine the degree of pulpal anesthesia obtained with a primary infiltration of 1 cartridge of articaine in the incisive/mental nerve region of the mandibular second premolar and to determine the anesthetic efficacy of a repeat artica...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2012.11.039
更新日期:2013-03-01 00:00:00
abstract::A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81411-1
更新日期:1992-04-01 00:00:00
abstract::This study investigated the retreatment effectiveness of .04 Taper nickel-titanium rotary ProFiles. Thirty extracted single-rooted anterior teeth were instrumented and obturated with gutta-percha/Roth's Sealer using lateral condensation. They were distributed into three groups of 10 each. Retreatment for group A was d...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200002000-00010
更新日期:2000-02-01 00:00:00
abstract::Studies of endodontic outcomes suggest that fracture of the tooth after endodontic therapy may be a greater problem than endodontic reinfection. Immediate full coverage with or without a post and core is the best way to prevent fracture. Unfortunately, in many population areas, due predominantly to cost, this restorat...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2019.12.012
更新日期:2020-04-01 00:00:00
abstract:INTRODUCTION:Surgical interventions such as tooth extraction increase the chances of developing osteonecrosis of the jaw in patients receiving bisphosphonates (BPs) for the treatment of bone-related diseases. Tooth extraction is often performed to eliminate preexisting pathological inflammatory conditions that make the...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.07.020
更新日期:2016-11-01 00:00:00
abstract::The purpose of this in vitro study was to evaluate quantitatively the ability of four different filling materials to seal the orifices of root canals as a secondary seal after root canal therapy. Forty extracted human molar teeth were used. The top of pulp chambers and distal halves of the roots were removed using an ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200108000-00006
更新日期:2001-08-01 00:00:00
abstract::One hundred single-rooted teeth were instrumented with Lightspeed, Profile .04 Taper Series 29, and NT McXIM instruments according to the recommendations of the manufacturers, and Flex-R files using the balanced force technique. Groups were comparable with respect to average curvature, canal length, and major and mino...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80086-1
更新日期:1998-02-01 00:00:00
abstract:INTRODUCTION:This retrospective study aimed to analyze the effect of repairing endodontic access cavities with composite on the survival of single crowns and retainer restorations of fixed or removable dental prostheses. METHODS:Dental records of patients attending a university dental clinic were retrospectively scree...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.11.012
更新日期:2020-03-01 00:00:00