Abstract:
INTRODUCTION:The aim of this study was to examine whether Enterococcus faecalis isolates from endodontic patients (from saliva and from a root canal) are able to prevail against salivary bacteria when grown in coculture in a biofilm reactor. METHODS:Saliva that was tested to be free of E. faecalis was used as the inoculum. The fate of E. faecalis was examined by using culture techniques and fluorescence in situ hybridization (FISH). RESULTS:The root canal isolate accounted for 37.4% of the biofilm and 31.9% of the planktonic phase when examined by the culture technique, whereas the proportions examined by FISH showed 15.3% in the biofilm and 11.7% in the planktonic phase. The saliva isolate (as examined by the culture technique) accounted for 32.4% in the biofilm and 27.1% in the planktonic phase, respectively, compared with 14.1% in the biofilm and 9.5% in the planktonic phase when examined by FISH analysis. CONCLUSIONS:These results led to the suggestion that E. faecalis could persist in the biofilm of the human oral cavity. Because of the ubiquitous presence of E. faecalis, root canal infections may arise from different sources.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Al-Ahmad A,Müller N,Wiedmann-Al-Ahmad M,Sava I,Hübner J,Follo M,Schirrmeister J,Hellwig Edoi
10.1016/j.joen.2009.04.013subject
Has Abstractpub_date
2009-07-01 00:00:00pages
986-91issue
7eissn
0099-2399issn
1878-3554pii
S0099-2399(09)00357-4journal_volume
35pub_type
杂志文章abstract:INTRODUCTION:Elucidating the microbial ecology of endodontic infections (EIs) is a necessary step in developing effective intracanal antimicrobials. The aim of the present study was to investigate the bacterial composition of symptomatic and asymptomatic primary and persistent infections in a Greek population using hig...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.03.010
更新日期:2015-08-01 00:00:00
abstract:INTRODUCTION:This study aimed at evaluating the bond strength to root dentin of 2 mineral trioxide aggregate (MTA)-based sealers (Endo-CPM sealer and MTA Fillapex) and of 1 epoxy resin-based sealer (AH Plus sealer). METHODS:Forty-five extracted human teeth with single roots were prepared by using the step-back techniq...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.10.018
更新日期:2012-02-01 00:00:00
abstract::The purpose of this in vitro study was to evaluate the mineral contents of root-canal dentin before and after treatment with two commonly used gutta-percha solvents: chloroform and halothane. Twenty extracted human premolars, whose crowns and apical thirds had been removed, were used. Pulp tissues were removed and the...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200401000-00012
更新日期:2004-01-01 00:00:00
abstract::Biofilm mode of growth is a strategy in microorganisms to survive harsh growth conditions. Although previous studies have established the ability of Enterococcus faecalis to survive postendodontic environmental conditions, the effect of such conditions on the ultrastructural and physiochemical features of E. faecalis ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000164855.98346.fc
更新日期:2005-12-01 00:00:00
abstract:INTRODUCTION:The purpose of the study was to evaluate the effects of intracanal, intraoral, and extraoral cryotherapy on postoperative pain in molar teeth with symptomatic apical periodontitis. METHODS:A total of 100 patients were randomly distributed into 4 groups: control (without cryotherapy application), intracana...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2017.11.002
更新日期:2018-03-01 00:00:00
abstract::Black-pigmented bacteria (BPB) have been associated with endodontic infections. The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80268-4
更新日期:1999-06-01 00:00:00
abstract:INTRODUCTION:The effect of endodontic involvement on tooth loss has not been quantified, so the present study aimed to assess this relationship after controlling for other relevant risk factors for tooth loss. METHODS:We analyzed data from 791 participants (18,798 teeth) in the Veterans Affairs Dental Longitudinal Stu...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.08.051
更新日期:2010-12-01 00:00:00
abstract::The objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. DNA was extracted from 45 canal samples of root-filled teet...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.01.016
更新日期:2008-05-01 00:00:00
abstract:INTRODUCTION:It is important to develop an antimicrobial agent without any damage on dental pulp. In the present study, we examined whether pretreatment of bacterial lipopolysaccharides (LPS) with ozonated water (O(3)aq) improves LPS-induced responses of rat odontoblastic cell line, KN-3. METHODS:After the pretreatmen...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.01.016
更新日期:2009-05-01 00:00:00
abstract:INTRODUCTION:Interferon regulatory factor 8 (IRF8) is a critical transcription factor in innate immune responses that regulates the development and function of myeloid cells. Human periapical lesions are caused by endodontic microbial infections. However, the presence of IRF8 in human periapical lesions remains elusive...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.04.015
更新日期:2018-08-01 00:00:00
abstract:INTRODUCTION:This case report describes the effect of unintentionally extruded mineral trioxide aggregate (MTA) into periradicular lesions and tissues on the prognosis of periradicular healing in maxillary left central incisor with periradicular lesion. METHODS:A 17-year-old girl with a large periradicular lesion from...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.11.026
更新日期:2010-04-01 00:00:00
abstract:INTRODUCTION:The presence of cracks and fractures in teeth can pose difficulties in diagnosis, prognosis assessment, and treatment recommendations. When a tooth has no significant restorations or caries, whereby the pulp is nonvital in the absence of a luxation injury, it is suggested that this necrosis is likely cause...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.12.018
更新日期:2010-03-01 00:00:00
abstract::Removing posts from root canals prior to endodontic retreatment is often a challenge. The Gonon post removing system (Thomas Extracteur De Pivots; FFDM-Pneumat, Bourges, France) is safe and efficient and can be used for anterior, bicuspid, and even molar teeth. The Gonon system is illustrated with one clinical case. ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80200-6
更新日期:1989-11-01 00:00:00
abstract::Plasminogen activator converts plasminogen to plasmin, and plasmin activates the latent matrix metalloproteinases. Tissue plasminogen activator (t-PA) is one of the important proteolysis factors present in human inflamed tissues. However, few studies reported on the mechanisms of tissue destruction via a t-PA proteoly...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200302000-00007
更新日期:2003-02-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the antimicrobial effects of root canal sealers on Enterococcus faecalis biofilms in dentinal tubules by using a novel dentin infection model. METHODS:Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart inf...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.10.042
更新日期:2014-04-01 00:00:00
abstract:INTRODUCTION:Neuropeptides play an important role in inflammation and repair and have been implicated in mediating angiogenesis. Pulp fibroblasts express neuropeptide receptors, and the aim of this research was to investigate whether neuropeptides could regulate angiogenic growth factor expression in vitro METHODS:An ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.03.005
更新日期:2009-06-01 00:00:00
abstract:INTRODUCTION:Dental discoloration with use of materials containing bismuth oxide has been reported. It is postulated that the discoloration is a result of chemical interaction of bismuth oxide with dentin. The aim of the study was to analyze dental color alteration and the chemical interaction of bismuth oxide with the...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.01.044
更新日期:2014-08-01 00:00:00
abstract:INTRODUCTION:This review aimed to find the most effective oral premedication in reducing pain in adults after nonsurgical root canal therapy (NSRCT) using network meta-analysis. METHODS:The review protocol was registered in the PROSPERO database (CRD42017071899). A literature search was performed in the MEDLINE and EB...
journal_title:Journal of endodontics
pub_type: 杂志文章,meta分析
doi:10.1016/j.joen.2018.10.016
更新日期:2019-04-01 00:00:00
abstract::The effect of two different root canal cements and removal of the smear layer on the apical seal in roots obturated with Thermafil obturators was tested. Thirty extracted human mandibular first molars with 60 canals (mesial roots) were instrumented with the use of a step-back technique. Teeth were placed into 6 groups...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80131-3
更新日期:1998-05-01 00:00:00
abstract::The purpose of this study was to evaluate a new periapical index based on cone beam computed tomography (CBCT) for identification of apical periodontitis (AP). The periapical index proposed in this study (CBCTPAI) was developed on the basis of criteria established from measurements corresponding to periapical radioluc...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.08.013
更新日期:2008-11-01 00:00:00
abstract:INTRODUCTION:There are no long-term, prospective clinical studies assessing outcomes of endodontically treated cracked teeth with radicular extensions. The purpose of this prospective study was to examine the 2- to 4-year success and survival rates of endodontically treated, coronally restored, cracked teeth, specifica...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.015
更新日期:2019-07-01 00:00:00
abstract::The purpose of this study was to compare the in vitro sealing capacity of five materials, each used as a temporary sealing agent for the walking bleach technique. All teeth received traditional biomechanical root canal instrumentation, after which the walking bleach agent was placed in the pulp chamber space. The occl...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200012000-00011
更新日期:2000-12-01 00:00:00
abstract::There is an increasing interest in the utility of dental pulp stem cells (DPSCs) for dentin regeneration. The mechanisms involved in DPSC differentiation remain poorly understood. The purpose of the study was to investigate the mineralization capacity of human dental pulp cells (DPCs) and identify potential markers fo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.02.009
更新日期:2007-06-01 00:00:00
abstract::This study assessed in vitro the diagnostic ability of cone beam computed tomography (CBCT) scans with different voxel resolutions in the detection of simulated external root resorption (ERR). For that purpose, 59 teeth were viewed through iCAT tomography (Imaging Sciences International, Inc, Hatfield, PA) following t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.11.005
更新日期:2009-02-01 00:00:00
abstract::Propolis, a flavonoid-rich product of honey comb, exhibits antibacterial and anti-inflammatory properties. In this study, we examined the tolerance of fibroblasts of the periodontal ligament (PDL) and dental pulp to propolis and compared with that of calcium hydroxide in vitro. Cells from human dental pulp and PDL wer...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200405000-00012
更新日期:2004-05-01 00:00:00
abstract::Intracoronal bleaching of pulpless teeth with 30% hydrogen peroxide may result in external cervical root resorption. One of several suggested etiological factors associated with this complication is the pH of the bleaching materials. Because the available data on the pH values of bleaching materials were conflicting, ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81988-6
更新日期:1991-08-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to compare the efficacy of conventional syringe, ultrasonic, EndoVac (Discus Dental, Culver City, CA), and Self-Adjusting File (SAF) (Re-Dent-Nova, Ra'nana, Israel) irrigation systems in removing calcium hydroxide (Ca[OH]2) from simulated root canal irregularities. METHODS:The ro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.10.019
更新日期:2014-03-01 00:00:00
abstract::This study compared the apical seal produced by four obturation techniques. Sixty-four extracted human teeth were prepared and obturated using lateral condensation of gutta-percha that was either unmodified or was dipped in chloroform, eucalyptol, or eucapercha paste. After storage in normal saline and 0.02% azide sol...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80158-X
更新日期:1989-12-01 00:00:00
abstract:INTRODUCTION:Pulp capping materials allow healing of injured pulp with a layer of reparative dentin. Glucose is needed to cure the injured area. Glucose is transported by glucose transporter (Glut) 2 and Glut4, which are transmembrane proteins that act as gatekeepers. We hypothesized that the transport of glucose via G...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.10.003
更新日期:2020-01-01 00:00:00
abstract::This in vitro study was carried out to determine (1) the hydrophobicity of selected oral bacteria, (2) the influence of growth media (saliva and serum) and mode of growth (planktonic or biofilm) on the hydrophobicity of Enterococcus faecalis, and (3) the influence of growth media and conditioning fluids on the adheren...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.07.035
更新日期:2007-12-01 00:00:00