Bioactivity-integrated UPLC/Q-TOF-MS of Danhong injection to identify NF-κB inhibitors and anti-inflammatory targets based on endothelial cell culture and network pharmacology.


ETHNOPHARMACOLOGICAL RELEVANCE:Danhong injection (DHI) is a traditional Chinese medicine injection that has been widely used in therapy for cardiovascular diseases. However, neither its mechanism nor its active constituents are clearly understood. AIM OF THE STUDY:Our research aimed at identifying the anti-inflammatory ingredients and mechanism of DHI by combining high-throughput screening (HTS) with network pharmacology analysis. MATERIALS AND METHODS:The human endothelial cell line EAhy926 was cultured in vitro. Methyl thiazolyltetrazolium (MTT) and lactate dehydrogenase (LDH) assays were performed to detect cell viability. The expression of Bcl-2 and Bax, interleukin-6 (IL-6), inhibitor of nuclear factor kappa-B kinase (IKK), phosphorylated IKK, phosphorylated NF-κB and phosphorylated IκB-α from the supernatant were determined. Then, we constructed an assay system combining ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) with an NF-κB activity luciferase reporter to screen DHI for essential anti-inflammatory components, and the results were verified using network pharmacology. RESULTS:DHI could significantly suppress inflammatory responses, and the mechanism may be via an NF-κB-dependent pathway. We found nine potential anti-inflammatory ingredients: danshensu, protocatechuic acid, protocatechuic aldehyde, caffeic acid, hydroxysafflor yellow A, safflor yellow A, salvianolic acid A salvianolic acid B and salvianolic acid C. Among these compounds, the NF-κB inhibitory activity of SAC is reported here for the first time. CONCLUSIONS:DHI plays an important role in suppressing inflammatory responses through inhibiting the NF-κB signaling pathway. The potential NF-κB inhibitors in DHI contribute to the cross-talk of multiple targets in anti-inflammation.


J Ethnopharmacol


Jiang X,Lv B,Li P,Ma X,Wang T,Zhou Q,Wang X,Gao X




Has Abstract


2015-11-04 00:00:00












  • [General pharmacologic inventory of Rwandese medicinal plants].

    abstract::A pharmacological screening was conducted on 101 methanolic extracts from 31 medicinal plants chosen among the more widely used plants in Rwandese traditional medicine. Twenty four extracts from 13 plants have shown spasmolytic properties, while 35 extracts from 15 plants contain spasmodic active principles. Ten extra...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Chagnon M

    更新日期:1984-12-01 00:00:00

  • Screening for cytotoxic activity of plants used in traditional medicine.

    abstract::A total of 260 extracts have been prepared from 75 medicinal plant species collected in Africa, Panama and Mauritius. Three different concentrations of each extract have been screened in vitro for cytotoxic activity using a colorimetric assay to determine cell survival of human colon carcinoma Co115 cells. Fifteen pla...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Chapuis JC,Sordat B,Hostettmann K

    更新日期:1988-07-01 00:00:00

  • Cytotoxicity of South-African medicinal plants towards sensitive and multidrug-resistant cancer cells.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Traditional medicine plays a major role for primary health care worldwide. Cancer belongs to the leading disease burden in industrialized and developing countries. Successful cancer therapy is hampered by the development of resistance towards established anticancer drugs. AIM:In the pres...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Saeed MEM,Meyer M,Hussein A,Efferth T

    更新日期:2016-06-20 00:00:00

  • Ethnobotanical inventory of medicinal plants used by the Guaymi Indians in western Panama. Part II.

    abstract::A list of newly identified plants is presented to supplement Part I (Joly et al. (1987) Journal of Ethnopharmacology 20, 145-171). A comparative analysis is made between our work and two other recent inventories of plants used by the Guaymi Indians of Panama and Costa Rica. As in Part I, the results of a literature su...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Joly LG,Guerra S,Séptimo R,Solís PN,Correa MD,Gupta MP,Levy S,Sandberg F,Perera P

    更新日期:1990-02-01 00:00:00

  • Comparative in vitro bioactivities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells.

    abstract:AIM OF THE STUDY:Ardisia species, notably A. compressa, are used in some regions of the world as food or in traditional medicine for prevention and treatment of certain health conditions including liver disease. We investigated the chemical composition and relative anticancer potential of six Ardisia species [A. japoni...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Newell AM,Yousef GG,Lila MA,Ramírez-Mares MV,de Mejia EG

    更新日期:2010-08-09 00:00:00

  • In vivo and in vitro toxicological evaluation of the hydroalcoholic leaf extract of Ageratum conyzoides L. (Asteraceae).

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:In African traditional medicine, Ageratum conyzoides has been used as purgative, febrifuge, anti-ulcer and wound dressing. To date there is no safety information about long term use of Ageratum conyzoides which contains pyrrolizidine alkaloids, a class of hepatotoxic and carcinogenic phyt...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Diallo A,Eklu-Gadegbeku K,Amegbor K,Agbonon A,Aklikokou K,Creppy E,Gbeassor M

    更新日期:2014-09-11 00:00:00

  • Dual mechanisms of a Sri Lankan traditional polyherbal mixture in the improvement of pancreatic beta cell functions and restoration of lipoprotein alterations in streptozotocin induced diabetic rats.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Traditional polyherbal preparations have been utilized in Sri Lanka since ancient times and have gained a wide acceptance throughout the country. Although an extensive body of evidence supports the use of traditional herbal mixtures in the treatment of diabetes mellitus, only a few polyhe...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Liyanagamage DSNK,Jayasinghe S,Attanayake AP,Karunaratne V

    更新日期:2021-03-01 00:00:00

  • Ethnobotanical study of indigenous knowledge on medicinal and nutritious plants used to manage opportunistic infections associated with HIV/AIDS in western Uganda.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Traditional medicine plays an important role in the daily lives of the people of Uganda to treat a wide range of health problems. Our study presents results of an ethnobotanical inventory conducted to identify and document medicinal and nutritional plants used in the management of opportu...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Mugisha MK,Asiimwe S,Namutebi A,Borg-Karlson AK,Kakudidi EK

    更新日期:2014-08-08 00:00:00

  • Inhalation of Cedrus atlantica essential oil alleviates pain behavior through activation of descending pain modulation pathways in a mouse model of postoperative pain.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Cedrus atlantica essential oil (CaEO) presents analgesic and anti-inflammatory sedative properties. However, it remains unknown whether CaEO alleviates acute postoperative pain. MATERIALS AND METHODS:Here, we investigated the effect of CaEO on postoperative pain and its mechanisms relate...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Martins DF,Emer AA,Batisti AP,Donatello N,Carlesso MG,Mazzardo-Martins L,Venzke D,Micke GA,Pizzolatti MG,Piovezan AP,dos Santos AR

    更新日期:2015-12-04 00:00:00

  • Pharmacologic effects of an aqueous extract of Rhigiocarya racemifera.

    abstract::Several pharmacologic studies were carried out with an aqueous extract of Rhigiocarya racemifera after phytochemical tests revealed the main constituents to be glycosides, saponins and tannins. Studies on intact mice showed that the extract has an intraperitoneal LD50 of 142 mg/kg and that 50 mg/kg of the extract redu...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Aguwa CN

    更新日期:1986-02-01 00:00:00

  • A review of Algerian medicinal plants used in the treatment of diabetes.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Plants are traditionally used in Algeria to treat many disorders, including diabetes mellitus. Knowledge of the plants that are used may provide insight on their properties, for further exploration. This study reviewed all the available published and unpublished reports concerning the use...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Hamza N,Berke B,Umar A,Cheze C,Gin H,Moore N

    更新日期:2019-06-28 00:00:00

  • Protective effect of a polyphenolic rich extract from Magnolia officinalis bark on influenza virus-induced pneumonia in mice.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Magnolia officinalis bark is used in traditional Chinese medicine for the treatment of cough, colds, fever, chronic bronchitis and stomach ailments. AIM OF THE STUDY:To investigate therapeutic effects of polyphenol rich extract from M. officinalis bark (MPE) in influenza virus A-infected...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Wu XN,Yu CH,Cai W,Hua J,Li SQ,Wang W

    更新日期:2011-03-08 00:00:00

  • Dalspinin isolated from Spermacoce hispida (Linn.) protects H9c2 cardiomyocytes from hypoxic injury by modulating oxidative stress and apoptosis.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Spermacoce hispida (S.hispida), a potential medicinal plant has been traditionally used as an antibacterial, antieczemic, antihypertensive, antidiabetic and antihyperlipidemic agent. Although, this plant has been claimed to protect against oxidative injury and inflammatory conditions in r...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Lakshmi Sundaram R,Vasanthi HR

    更新日期:2019-09-15 00:00:00

  • Baicalein attenuates α-synuclein aggregation, inflammasome activation and autophagy in the MPP+-treated nigrostriatal dopaminergic system in vivo.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Neuroinflammation, oxidative stress, and protein aggregation form a vicious cycle in the pathophysiology of Parkinson's disease (PD); activated microglia is the main location of neuroinflammation. A Chinese medicine book, "Shanghan Lun", known as the "Treatises on Cold damage Diseases" ha...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Hung KC,Huang HJ,Wang YT,Lin AM

    更新日期:2016-12-24 00:00:00

  • 1-Triacontanol cerotate; isolated from Marsilea quadrifolia Linn. ameliorates reactive oxidative damage in the frontal cortex and hippocampus of chronic epileptic rats.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Marsilea quadrifolia Linn. (MQ) has been used for insomnia and epileptic disorders in traditional Indian medicine. The present study is to isolate the active component responsible for antiepileptic property of MQ by evaluating its ability to minimize the reactive oxidative damage in brain...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Snehunsu A,Ghosal C,Kandwal M,Yadav PK,Nayak BS,Rao KR,Kamath SU,Sahoo P,Srinivasan KK,Naduvil Narayanan S,Kumar S,Joseph A

    更新日期:2015-08-22 00:00:00

  • Myorelaxant Activity of essential oil from Origanum majorana L. on rat and rabbit.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Origanum majorana L. (Lamiaceae) was usually used in Moroccan folk medicine to treat infantile colic and abdominal discomfort. MATERIALS AND METHODS:The essential oil from the aerial part of the dry Origanum majorana L. (EOOM) was obtained through hydro distillation and analyzed by gas c...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Makrane H,Aziz M,Berrabah M,Mekhfi H,Ziyyat A,Bnouham M,Legssyer A,Elombo FK,Gressier B,Eto B

    更新日期:2019-01-10 00:00:00

  • Anti-venom potential of butanolic extract of Eclipta prostrata against Malayan pit viper venom.

    abstract::The butanolic and purified butanolic extracts (PBEs) of Eclipta prostrata were evaluated for their anti-venom potential. Inhibition of lethal, hemorrhagic, proteolytic, and phospholipase A2 activities of Calloselasma rhodostoma (Malayan pit viper (MPV)) venom by these extracts were determined. Demethylwedelolactone wa...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Pithayanukul P,Laovachirasuwan S,Bavovada R,Pakmanee N,Suttisri R

    更新日期:2004-02-01 00:00:00

  • In vitro antiplasmodial activity of 18 plants used in Congo Brazzaville traditional medicine.

    abstract::Sixty-six extracts of 18 plants commonly used by traditional healers in Congo Brazzaville for the treatment of malaria have been investigated for in vitro antiplasmodial activity. Ethanolic and dichloromethane extracts of 7 among the 18 studied plants were moderately active (10 microg/ml

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Mbatchi SF,Mbatchi B,Banzouzi JT,Bansimba T,Nsonde Ntandou GF,Ouamba JM,Berry A,Benoit-Vical F

    更新日期:2006-03-08 00:00:00

  • Ethnomedicinal uses of Momordicacharantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activity.

    abstract::Investigation of the traditional uses of Momordica charantia (Cucurbitaceae) in Togo (West Africa) showed that it is one of the most important local medicinal plants both for ritual and ethnomedical practices. There was a high degree of consensus (>50%) for use in the treatment of gastrointestinal and viral disease am...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Beloin N,Gbeassor M,Akpagana K,Hudson J,de Soussa K,Koumaglo K,Arnason JT

    更新日期:2005-01-04 00:00:00

  • Zingansikpoongtang modulates beta-amyloid and IL-1beta-induced cytokine production and cyclooxygenase-2 expression in human astrocytoma cells U373MG.

    abstract::Zingansikpoongtang (ZST) is a Korean herbal prescription, which has been successfully applied for the various neurodegenerative diseases. However, its effect remains unknown in the experimental models. In this study, we examined the effect of ZST on production of interleukin (IL)-6 and IL-8, and expression of cyclooxy...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Kim SJ,Jeong HJ,Lee KM,Ryu HH,Lee KS,Moon BS,Cho KH,Kim HM

    更新日期:2005-01-04 00:00:00

  • Antihyperglycemic, antihyperlipidemic and antioxidant effects of ethanol and aqueous extracts of Cyclocarya paliurus leaves in type 2 diabetic rats.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Cyclocarya paliurus (CP) Batal., the sole species in its genus and native to China, is a herbal tea, which has been traditionally used in the folk medicine for the treatment of diabetes and hyperlipidemia in China. To evaluate the antihyperglycemic, antihyperlipidemic and antioxidant effe...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Wang Q,Jiang C,Fang S,Wang J,Ji Y,Shang X,Ni Y,Yin Z,Zhang J

    更新日期:2013-12-12 00:00:00

  • Soma siddhas and alchemical enlightenment: psychedelic mushrooms in Buddhist tradition.

    abstract::In the legendary biographies of some Buddhist adepts from the 2nd- and 9th-centuries there are some clues which can be interpreted to reveal that the adepts were consuming psychedelic Amanita muscaria, 'fly agaric', mushrooms to achieve enlightenment. This secret ingredient in the alchemical elixir they used to attain...

    journal_title:Journal of ethnopharmacology

    pub_type: 历史文章,杂志文章


    authors: Hajicek-Dobberstein S

    更新日期:1995-10-01 00:00:00

  • Antiosteoporotic chemical constituents from Er-Xian Decoction, a traditional Chinese herbal formula.

    abstract:AIM OF THE STUDY:Er Xian Decoction (EXD), a traditional Chinese medicine formula, has long been used for the treatment of osteoporosis and menopausal syndrome in China. The present study was designed to investigate the antiosteoporotic constituents of EXD, and evaluate their antiosteoporotic effects in ovariectomized r...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Qin L,Han T,Zhang Q,Cao D,Nian H,Rahman K,Zheng H

    更新日期:2008-07-23 00:00:00

  • Anti-inflammatory activity of the bark of Hippocratea excelsa.

    abstract::The ethanol extract of the plant Hippocratea excelsa was examined for its anti-inflammatory effects using several animal models. It produced significant inhibition of carrageenan-induced paw edema and reduced the weight of cotton pellet-induced granuloma at doses of 25-100 mg/kg. The extract was found to exert a prote...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Perez RM,Perez S,Zavala MA,Salazar M

    更新日期:1995-07-07 00:00:00

  • Garcinia kola aqueous suspension prevents cerebellar neurodegeneration in long-term diabetic rat - a type 1 diabetes mellitus model.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:The development of compounds able to improve metabolic syndrome and mitigate complications caused by inappropriate glycemic control in type 1 diabetes mellitus is challenging. The medicinal plant with established hypoglycemic properties Garcinia kola Heckel might have the potential to mit...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Farahna M,Seke Etet PF,Osman SY,Yurt KK,Amir N,Vecchio L,Aydin I,Aldebasi YH,Sheikh A,Chijuka JC,Kaplan S,Adem A

    更新日期:2017-01-04 00:00:00

  • The influence of Sutherlandia frutescens on adrenal steroidogenic cytochrome P450 enzymes.

    abstract:AIM OF THE STUDY:The aim of this study was to investigate whether Sutherlandia frutescens, subsp. microphylla (family: Fabaceae/Leguminosa), which is traditionally used to treat symptoms of chronic stress generally associated with increased circulating glucocorticoids, influences the biosynthesis of these glucocorticoi...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Prevoo D,Swart P,Swart AC

    更新日期:2008-06-19 00:00:00

  • Antiplasmodial activity of Mezoneuron benthamianum leaves and identification of its active constituents.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Decoctions of the leaves of M. benthamianum Baill. are used by traditional healers in Guinea to treat malaria and this use was validated by a preliminary clinical assay. AIM OF THE STUDY:To evaluate the in vitro antiplasmodial activity and to identify active compounds from extracts of M....

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Jansen O,Tchinda AT,Loua J,Esters V,Cieckiewicz E,Ledoux A,Toukam PD,Angenot L,Tits M,Balde AM,Frédérich M

    更新日期:2017-05-05 00:00:00

  • Further studies on the hepatoprotective effects of Cochlospermum tinctorium rhizomes.

    abstract::The hepatoprotective activity of the rhisome of Cochlospermum tinctorium was investigated using carbon tetrachloride toxicity on mouse and tert-butyl hydroperoxide in vitro induction of lipid peroxidation and hepatocyte lysis. Aqueous, hydro-ethanolic and ethanolic extracts showed significant dose-dependent hepatoprot...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Diallo B,Vanhaelen-Fastre R,Vanhaelen M,Fiegel C,Joyeux M,Roland A,Fleurentin J

    更新日期:1992-04-01 00:00:00

  • The effects of oridonin on cell growth, cell cycle, cell migration and differentiation in melanoma cells.

    abstract::Oridonin, a diterpenoid isolated from Rabdosia rubescens (Hemsl.) Hara, is currently one of the most important traditional Chinese herbal medicines. We investigated the anti-proliferation effect of oridonin on the cultured murine melanoma cell line K1735M2. The growth inhibition activity of oridonin for K1735M2 cells ...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Ren KK,Wang HZ,Xie LP,Chen DW,Liu X,Sun J,Nie YC,Zhang RQ

    更新日期:2006-01-16 00:00:00

  • In vivo disposition and metabolism of madecassoside, a major bioactive constituent in Centella asiatica (L.) Urb.

    abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Centella asiatica (L.) Urb. herb is frequently used in traditional Chinese medicine for many indications, such as traumatic injuries, keloid and scar. Madecassoside is the main active ingredient of this herb drug with higher content than other triterpenoid constituents. Understandings of ...

    journal_title:Journal of ethnopharmacology

    pub_type: 杂志文章


    authors: Leng DD,Han WJ,Rui Y,Dai Y,Xia YF

    更新日期:2013-11-25 00:00:00