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Anti-angiogenic effect of furanodiene on HUVECs in vitro and on zebrafish in vivo.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:Furanodiene is an active ingredient of the traditional Chinese medicine, Rhizoma Curcumae, commonly used for the treatment of cancer in China. AIM OF THE STUDY:To investigate the anti-cancer property of Rhizoma Curcumae, this study describes the anti-angiogenic activities of furanodiene in human umbilical vein endothelial cells (HUVECs) in vitro and in zebrafish in vivo. MATERIALS AND METHODS:HUVECs were treated with different doses of furanodiene in the presence or absence of vascular endothelial growth factor (VEGF). The anti-proliferative effect of furanodiene was measured using the XTT assay. The anti-migration and anti-invasion activities of this compound were investigated with a wound-healing migration model and a three-dimensional cell invasion model, respectively. The effects of furanodiene on HUVEC differentiation were assessed by in vitro tube formation in Matrigel™. The expression of related proteins was detected by Western blot. Morphological observations of zebrafish were evaluated in transgenic Tg (fli1: EGFP) zebrafish embryos. RESULTS:Our results showed that furanodiene exposure could significantly inhibit the proliferation of HUVECs in a dose-dependent manner and inhibit VEGF-induced proliferation at a low dose. Relative to the VEGF-induced control, the number of invading and migrating cells was significantly reduced in the furanodiene-treated groups. Furanodiene also dramatically suppressed tube formation and p-Akt (Ser473), p-Erk 1/2 (Thr202/Tyr204), ICAM-1, p-p85 (Ser428) as well as p85 protein expression. Furthermore, exposure to furanodiene inhibited angiogenesis in the zebrafish model. CONCLUSIONS:This study demonstrated that furanodiene exposure exhibits a potential anti-angiogenic effect through suppression of endothelial cell growth, invasion, migration and tube formation via regulation of the PI3K pathway. This potential anti-angiogenic effect of furanodiene may play an important role in the anti-tumor activity of the traditional Chinese medicine, Rhizoma Curcumae.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Zhong ZF,Hoi PM,Wu GS,Xu ZT,Tan W,Chen XP,Cui L,Wu T,Wang YT

doi

10.1016/j.jep.2011.08.052

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

721-7

issue

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(11)00625-8

journal_volume

141

pub_type

杂志文章

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Anti-angiogenic effect of furanodiene on HUVECs in vitro and on zebrafish in vivo.