当前位置： SCI文献检索 > NATURE MEDICINE期刊下所有文献 > In vivo imaging and tracking of host-microbiota interactions via metabolic labeling of gut anaerobic bacteria.

In vivo imaging and tracking of host-microbiota interactions via metabolic labeling of gut anaerobic bacteria.

Abstract：

:The intestine is densely populated by anaerobic commensal bacteria. These microorganisms shape immune system development, but understanding of host-commensal interactions is hampered by a lack of tools for studying the anaerobic intestinal environment. We applied metabolic oligosaccharide engineering and bioorthogonal click chemistry to label various commensal anaerobes, including Bacteroides fragilis, a common and immunologically important commensal. We studied the dissemination of B. fragilis after acute peritonitis and characterized the interactions of the intact microbe and its polysaccharide components in myeloid and B cell lineages. We were able to assess the distribution and colonization of labeled B. fragilis along the intestine, as well as niche competition after coadministration of multiple species of the microbiota. We also fluorescently labeled nine additional commensals (eight anaerobic and one microaerophilic) from three phyla common in the gut--Bacteroidetes, Firmicutes and Proteobacteria--as well as one aerobic pathogen (Staphylococcus aureus). This strategy permits visualization of the anaerobic microbial niche by various methods, including intravital two-photon microscopy and non-invasive whole-body imaging, and can be used to study microbial colonization and host-microbe interactions in real time.

journal_name

Nat Med

journal_title

Nature medicine

authors

Geva-Zatorsky N,Alvarez D,Hudak JE,Reading NC,Erturk-Hasdemir D,Dasgupta S,von Andrian UH,Kasper DL

doi

10.1038/nm.3929

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

1091-100

issue

9

eissn

1078-8956

issn

1546-170X

pii

nm.3929

journal_volume

21

pub_type

杂志文章

相关文献

NATURE MEDICINE文献大全
  • Cleavage of tau by asparagine endopeptidase mediates the neurofibrillary pathology in Alzheimer's disease.

    abstract：:Neurofibrillary tangles (NFTs), composed of truncated and hyperphosphorylated tau, are a common feature of numerous aging-related neurodegenerative diseases, including Alzheimer's disease (AD). However, the molecular mechanisms mediating tau truncation and aggregation during aging remain elusive. Here we show that asp...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.3700

    authors： Zhang Z,Song M,Liu X,Kang SS,Kwon IS,Duong DM,Seyfried NT,Hu WT,Liu Z,Wang JZ,Cheng L,Sun YE,Yu SP,Levey AI,Ye K

    更新日期：2014-11-01 00:00:00

  • HRDetect is a predictor of BRCA1 and BRCA2 deficiency based on mutational signatures.

    abstract：:Approximately 1-5% of breast cancers are attributed to inherited mutations in BRCA1 or BRCA2 and are selectively sensitive to poly(ADP-ribose) polymerase (PARP) inhibitors. In other cancer types, germline and/or somatic mutations in BRCA1 and/or BRCA2 (BRCA1/BRCA2) also confer selective sensitivity to PARP inhibitors....

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.4292

    authors： Davies H,Glodzik D,Morganella S,Yates LR,Staaf J,Zou X,Ramakrishna M,Martin S,Boyault S,Sieuwerts AM,Simpson PT,King TA,Raine K,Eyfjord JE,Kong G,Borg Å,Birney E,Stunnenberg HG,van de Vijver MJ,Børresen-Dale AL,Ma

    更新日期：2017-04-01 00:00:00

  • Airway epithelial gene expression in the diagnostic evaluation of smokers with suspect lung cancer.

    abstract：:Lung cancer is the leading cause of death from cancer in the US and the world. The high mortality rate (80-85% within 5 years) results, in part, from a lack of effective tools to diagnose the disease at an early stage. Given that cigarette smoke creates a field of injury throughout the airway, we sought to determine i...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1556

    authors： Spira A,Beane JE,Shah V,Steiling K,Liu G,Schembri F,Gilman S,Dumas YM,Calner P,Sebastiani P,Sridhar S,Beamis J,Lamb C,Anderson T,Gerry N,Keane J,Lenburg ME,Brody JS

    更新日期：2007-03-01 00:00:00

  • The natural killer T-cell ligand alpha-galactosylceramide prevents autoimmune diabetes in non-obese diabetic mice.

    abstract：:Diabetes in non-obese diabetic (NOD) mice is mediated by pathogenic T-helper type 1 (Th1) cells that arise because of a deficiency in regulatory or suppressor T cells. V alpha 14-J alpha 15 natural killer T (NKT) cells recognize lipid antigens presented by the major histocompatibility complex class I-like protein CD1d...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm0901-1052

    authors： Hong S,Wilson MT,Serizawa I,Wu L,Singh N,Naidenko OV,Miura T,Haba T,Scherer DC,Wei J,Kronenberg M,Koezuka Y,Van Kaer L

    更新日期：2001-09-01 00:00:00

  • Marked amelioration of established collagen-induced arthritis by treatment with antibodies to CD23 in vivo.

    abstract：:CD23 is a low-affinity receptor for immunoglobulin E (IgE) expressed by a variety of haematopoietic cells. Proteolytic cleavage of the transmembrane receptor generates soluble forms, which can be detected in biological fluids. CD23 regulates many functional aspects of immune cells, both in its cell-associated and solu...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm0895-781

    authors： Plater-Zyberk C,Bonnefoy JY

    更新日期：1995-08-01 00:00:00

  • Near real-time intraoperative brain tumor diagnosis using stimulated Raman histology and deep neural networks.

    abstract：:Intraoperative diagnosis is essential for providing safe and effective care during cancer surgery1. The existing workflow for intraoperative diagnosis based on hematoxylin and eosin staining of processed tissue is time, resource and labor intensive2,3. Moreover, interpretation of intraoperative histologic images is de...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/s41591-019-0715-9

    authors： Hollon TC,Pandian B,Adapa AR,Urias E,Save AV,Khalsa SSS,Eichberg DG,D'Amico RS,Farooq ZU,Lewis S,Petridis PD,Marie T,Shah AH,Garton HJL,Maher CO,Heth JA,McKean EL,Sullivan SE,Hervey-Jumper SL,Patil PG,Thompson BG

    更新日期：2020-01-01 00:00:00

  • The pros and cons of drug 'trafficking'.

    abstract：:Internalization of targeted therapeutics is often needed for efficacy, but also alters drug penetration of a tissue. A new model explores the trade-offs of intracellular drug trafficking. ...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1195-1135

    authors： Stokes CL

    更新日期：1995-11-01 00:00:00

  • Natural killer cells migrate into and control simian immunodeficiency virus replication in lymph node follicles in African green monkeys.

    abstract：:Natural killer (NK) cells play an essential role in antiviral immunity, but knowledge of their function in secondary lymphoid organs is incomplete. Lymph node follicles constitute a major viral reservoir during infections with HIV-1 and simian immunodeficiency virus of macaques (SIVmac). In contrast, during nonpathoge...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.4421

    authors： Huot N,Jacquelin B,Garcia-Tellez T,Rascle P,Ploquin MJ,Madec Y,Reeves RK,Derreudre-Bosquet N,Müller-Trutwin M

    更新日期：2017-11-01 00:00:00

  • Cidea is an essential transcriptional coactivator regulating mammary gland secretion of milk lipids.

    abstract：:Adequate lipid secretion by mammary glands during lactation is essential for the survival of mammalian offspring. However, the mechanism governing this process is poorly understood. Here we show that Cidea is expressed at high levels in lactating mammary glands and its deficiency leads to premature pup death as a resu...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.2614

    authors： Wang W,Lv N,Zhang S,Shui G,Qian H,Zhang J,Chen Y,Ye J,Xie Y,Shen Y,Wenk MR,Li P

    更新日期：2012-01-15 00:00:00

  • Therapeutic differentiation and maturation of lymphatic vessels after lymph node dissection and transplantation.

    abstract：:Surgery or radiation therapy of metastatic cancer often damages lymph nodes, leading to secondary lymphedema. Here we show, using a newly established mouse model, that collecting lymphatic vessels can be regenerated and fused to lymph node transplants after lymph node removal. Treatment of lymph node-excised mice with...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1689

    authors： Tammela T,Saaristo A,Holopainen T,Lyytikkä J,Kotronen A,Pitkonen M,Abo-Ramadan U,Ylä-Herttuala S,Petrova TV,Alitalo K

    更新日期：2007-12-01 00:00:00

  • Targeting exogenous genes to tumor angiogenesis by transplantation of genetically modified hematopoietic stem cells.

    abstract：:Angiogenic tumor vessels are promising targets for the activity and the selective delivery of cancer therapeutics. The bone marrow contributes different cell types to the tumor stroma, including hematopoietic cells and, as recently suggested, vascular endothelial cells (ECs). Thus, transplantation of genetically modif...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm871

    authors： De Palma M,Venneri MA,Roca C,Naldini L

    更新日期：2003-06-01 00:00:00

  • Interstitial cells of Cajal generate a rhythmic pacemaker current.

    abstract：:Networks of interstitial cells of Cajal embedded in the musculature of the gastrointestinal tract are involved in the generation of electrical pacemaker activity for gastrointestinal motility. This pacemaker activity manifests itself as rhythmic slow waves in membrane potential, and controls the frequency and propagat...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm0798-848

    authors： Thomsen L,Robinson TL,Lee JC,Farraway LA,Hughes MJ,Andrews DW,Huizinga JD

    更新日期：1998-07-01 00:00:00

  • Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes.

    abstract：:Low oxygen tension influences tumor progression by enhancing angiogenesis; and histone deacetylases (HDAC) are implicated in alteration of chromatin assembly and tumorigenesis. Here we show induction of HDAC under hypoxia and elucidate a role for HDAC in the regulation of hypoxia-induced angiogenesis. Overexpressed wi...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/86507

    authors： Kim MS,Kwon HJ,Lee YM,Baek JH,Jang JE,Lee SW,Moon EJ,Kim HS,Lee SK,Chung HY,Kim CW,Kim KW

    更新日期：2001-04-01 00:00:00

  • Myeloperoxidase acts as a profibrotic mediator of atrial fibrillation.

    abstract：:Observational clinical and ex vivo studies have established a strong association between atrial fibrillation and inflammation. However, whether inflammation is the cause or the consequence of atrial fibrillation and which specific inflammatory mediators may increase the atria's susceptibility to fibrillation remain el...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.2124

    authors： Rudolph V,Andrié RP,Rudolph TK,Friedrichs K,Klinke A,Hirsch-Hoffmann B,Schwoerer AP,Lau D,Fu X,Klingel K,Sydow K,Didié M,Seniuk A,von Leitner EC,Szoecs K,Schrickel JW,Treede H,Wenzel U,Lewalter T,Nickenig G,Zimmer

    更新日期：2010-04-01 00:00:00

  • Endogenous antigen processing drives the primary CD4+ T cell response to influenza.

    abstract：:By convention, CD4+ T lymphocytes recognize foreign and self peptides derived from internalized antigens in combination with major histocompatibility complex class II molecules. Alternative pathways of epitope production have been identified, but their contributions to host defense have not been established. We show h...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.3958

    authors： Miller MA,Ganesan AP,Luckashenak N,Mendonca M,Eisenlohr LC

    更新日期：2015-10-01 00:00:00

  • CCL27-CCR10 interactions regulate T cell-mediated skin inflammation.

    abstract：:The skin-associated chemokine CCL27 (also called CTACK, ALP and ESkine) and its receptor CCR10 (GPR-2) mediate chemotactic responses of skin-homing T cells in vitro. Here we report that most skin-infiltrating lymphocytes in patients suffering from psoriasis, atopic or allergic-contact dermatitis express CCR10. Epiderm...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm0202-157

    authors： Homey B,Alenius H,Müller A,Soto H,Bowman EP,Yuan W,McEvoy L,Lauerma AI,Assmann T,Bünemann E,Lehto M,Wolff H,Yen D,Marxhausen H,To W,Sedgwick J,Ruzicka T,Lehmann P,Zlotnik A

    更新日期：2002-02-01 00:00:00

  • The TAM receptor Mertk protects against neuroinvasive viral infection by maintaining blood-brain barrier integrity.

    abstract：:The TAM receptors Tyro3, Axl and Mertk are receptor tyrosine kinases that dampen host innate immune responses following engagement with their ligands Gas6 and Protein S, which recognize phosphatidylserine on apoptotic cells. In a form of apoptotic mimicry, many enveloped viruses display phosphatidylserine on the outer...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.3974

    authors： Miner JJ,Daniels BP,Shrestha B,Proenca-Modena JL,Lew ED,Lazear HM,Gorman MJ,Lemke G,Klein RS,Diamond MS

    更新日期：2015-12-01 00:00:00

  • Cancer's true breakthroughs.

    abstract：:A new regulatory pathway established last year allows drugs with dramatic early clinical promise to be expedited to the market quicker than ever before. To date, most of these 'breakthrough' designations have gone to cancer agents, raising the prospect of faster access to the latest lifesaving therapies for the estima...

    journal_title：Nature medicine

    pub_type： 新闻

    doi：10.1038/nm.3245

    authors： Dolgin E

    更新日期：2013-06-01 00:00:00

  • Real-time quantitative RT-PCR after laser-assisted cell picking.

    abstract：:The present study describes a technique for quantitation of mRNA in a few isotypic cells obtained from an intact organ structure by combining laser-assisted cell picking and real-time PCR. The microscopically controlled lasering of selected cells in stained tissue sections was applied to lung alveolar macrophages, whi...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/3327

    authors： Fink L,Seeger W,Ermert L,Hänze J,Stahl U,Grimminger F,Kummer W,Bohle RM

    更新日期：1998-11-01 00:00:00

  • Wireless bioresorbable electronic system enables sustained nonpharmacological neuroregenerative therapy.

    abstract：:Peripheral nerve injuries represent a significant problem in public health, constituting 2-5% of all trauma cases1. For severe nerve injuries, even advanced forms of clinical intervention often lead to incomplete and unsatisfactory motor and/or sensory function2. Numerous studies report the potential of pharmacologica...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/s41591-018-0196-2

    authors： Koo J,MacEwan MR,Kang SK,Won SM,Stephen M,Gamble P,Xie Z,Yan Y,Chen YY,Shin J,Birenbaum N,Chung S,Kim SB,Khalifeh J,Harburg DV,Bean K,Paskett M,Kim J,Zohny ZS,Lee SM,Zhang R,Luo K,Ji B,Banks A,Lee HM,Hua

    更新日期：2018-12-01 00:00:00

  • The long noncoding RNA Chaer defines an epigenetic checkpoint in cardiac hypertrophy.

    abstract：:Epigenetic reprogramming is a critical process of pathological gene induction during cardiac hypertrophy and remodeling, but the underlying regulatory mechanisms remain to be elucidated. Here we identified a heart-enriched long noncoding (lnc)RNA, named cardiac-hypertrophy-associated epigenetic regulator (Chaer), whic...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.4179

    authors： Wang Z,Zhang XJ,Ji YX,Zhang P,Deng KQ,Gong J,Ren S,Wang X,Chen I,Wang H,Gao C,Yokota T,Ang YS,Li S,Cass A,Vondriska TM,Li G,Deb A,Srivastava D,Yang HT,Xiao X,Li H,Wang Y

    更新日期：2016-10-01 00:00:00

  • Activators of the nuclear receptor PPARgamma enhance colon polyp formation.

    abstract：:A high-fat diet increases the risk of colon, breast and prostate cancer. The molecular mechanism by which dietary lipids promote tumorigenesis is unknown. Their effects may be mediated at least in part by the peroxisome proliferator-activated receptors (PPARs). These ligand-activated nuclear receptors modulate gene ex...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/2042

    authors： Saez E,Tontonoz P,Nelson MC,Alvarez JG,Ming UT,Baird SM,Thomazy VA,Evans RM

    更新日期：1998-09-01 00:00:00

  • Intravenous immunoglobulin ameliorates ITP via activating Fc gamma receptors on dendritic cells.

    abstract：:Despite a more than 20-year experience of therapeutic benefit, the relevant molecular and cellular targets of intravenous immunoglobulin (IVIg) in autoimmune disease remain unclear. Contrary to the prevailing theories of IVIg action in autoimmunity, we show that IVIg drives signaling through activating Fc gamma recept...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1416

    authors： Siragam V,Crow AR,Brinc D,Song S,Freedman J,Lazarus AH

    更新日期：2006-06-01 00:00:00

  • Genomic signatures to guide the use of chemotherapeutics.

    abstract：:Using in vitro drug sensitivity data coupled with Affymetrix microarray data, we developed gene expression signatures that predict sensitivity to individual chemotherapeutic drugs. Each signature was validated with response data from an independent set of cell line studies. We further show that many of these signature...

    journal_title：Nature medicine

    pub_type： 杂志文章,收录出版

    doi：10.1038/nm1491

    authors： Potti A,Dressman HK,Bild A,Riedel RF,Chan G,Sayer R,Cragun J,Cottrill H,Kelley MJ,Petersen R,Harpole D,Marks J,Berchuck A,Ginsburg GS,Febbo P,Lancaster J,Nevins JR

    更新日期：2006-11-01 00:00:00

  • VEGF: a modifier of the del22q11 (DiGeorge) syndrome?

    abstract：:Hemizygous deletion of chromosome 22q11 (del22q11) causes thymic, parathyroid, craniofacial and life-threatening cardiovascular birth defects in 1 in 4,000 infants. The del22q11 syndrome is likely caused by haploinsufficiency of TBX1, but its variable expressivity indicates the involvement of additional modifiers. Her...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm819

    authors： Stalmans I,Lambrechts D,De Smet F,Jansen S,Wang J,Maity S,Kneer P,von der Ohe M,Swillen A,Maes C,Gewillig M,Molin DG,Hellings P,Boetel T,Haardt M,Compernolle V,Dewerchin M,Plaisance S,Vlietinck R,Emanuel B,Gittenb

    更新日期：2003-02-01 00:00:00

  • A framework for understanding and targeting residual disease in oncogene-driven solid cancers.

    abstract：:Molecular targeted therapy has the potential to dramatically improve survival in patients with cancer. However, complete and durable responses to targeted therapy are rare in individuals with advanced-stage solid cancers. Even the most effective targeted therapies generally do not induce a complete tumor response, res...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm.4091

    authors： Bivona TG,Doebele RC

    更新日期：2016-05-05 00:00:00

  • Publisher Correction: Neuromodulation of lumbosacral spinal networks enables independent stepping after complete paraplegia.

    abstract：:In the version of this article originally published, Dimitry G. Sayenko's affiliations were not correct. The following affiliation for this author was missing: Department of Neurosurgery, Center for Neuroregeneration, Houston Methodist Research Institute, Houston, TX, USA. This affiliation has been added for the autho...

    journal_title：Nature medicine

    pub_type： 已发布勘误

    doi：10.1038/s41591-018-0248-7

    authors： Gill ML,Grahn PJ,Calvert JS,Linde MB,Lavrov IA,Strommen JA,Beck LA,Sayenko DG,Van Straaten MG,Drubach DI,Veith DD,Thoreson AR,Lopez C,Gerasimenko YP,Edgerton VR,Lee KH,Zhao KD

    更新日期：2018-12-01 00:00:00

  • TLR-dependent and TLR-independent pathways of type I interferon induction in systemic autoimmunity.

    abstract：:We formulate a two-phase paradigm of autoimmunity associated with systemic lupus erythematosus, the archetypal autoimmune disease. The initial Toll-like receptor (TLR)-independent phase is mediated by dendritic cell uptake of apoptotic cell debris and associated nucleic acids, whereas the subsequent TLR-dependent phas...

    journal_title：Nature medicine

    pub_type： 杂志文章,评审

    doi：10.1038/nm1590

    authors： Baccala R,Hoebe K,Kono DH,Beutler B,Theofilopoulos AN

    更新日期：2007-05-01 00:00:00

  • Somatic overgrowth associated with overexpression of insulin-like growth factor II.

    abstract：:Overexpression of the normally imprinted fetal insulin-like growth factor II (IGF2) has been implicated in the pathogenesis of the cancer-predisposing Beckwith-Wiedemann syndrome (BWS). We have detected constitutional relaxation of imprinting of IGF2 in four children with somatic overgrowth who do not show diagnostic ...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm0396-311

    authors： Morison IM,Becroft DM,Taniguchi T,Woods CG,Reeve AE

    更新日期：1996-03-01 00:00:00

  • TH17 cells contribute to uveitis and scleritis and are expanded by IL-2 and inhibited by IL-27/STAT1.

    abstract：:T-helper type 17 cells (T(H)17) are implicated in rodent models of immune-mediated diseases. Here we report their involvement in human uveitis and scleritis, and validate our findings in experimental autoimmune uveoretinitis (EAU), a model of uveitis. T(H)17 cells were present in human peripheral blood mononuclear cel...

    journal_title：Nature medicine

    pub_type： 杂志文章

    doi：10.1038/nm1585

    authors： Amadi-Obi A,Yu CR,Liu X,Mahdi RM,Clarke GL,Nussenblatt RB,Gery I,Lee YS,Egwuagu CE

    更新日期：2007-06-01 00:00:00