Abstract:
:A complex feedback mechanism between parathyroid hormone (PTH), 1,25(OH)2D3 (1,25D), and fibroblast growth factor 23 (FGF-23) maintains mineral homeostasis, in part by regulating calcium and phosphate absorption/reabsorption. Previously, we showed that 1,25D regulates mineral homeostasis by repressing dentin matrix protein 1 (DMP1) via the vitamin D receptor pathway. Similar to 1,25D, PTH may modulate DMP1, but the underlying mechanism remains unknown. Immortalized murine cementoblasts (OCCM.30), similar to osteoblasts and known to express DMP1, were treated with PTH (1-34). Real-time quantitative polymerase chain reaction (PCR) and Western blot revealed that PTH decreased DMP1 gene transcription (85%) and protein expression (30%), respectively. PTH mediated the downregulation of DMP1 via the cAMP/protein kinase A (PKA) pathway. Immunohistochemistry confirmed the decreased localization of DMP1 in vivo in cellular cementum and alveolar bone of mice treated with a single dose (50 µg/kg) of PTH (1-34). RNA-seq was employed to further identify patterns of gene expression shared by PTH and 1,25D in regulating DMP1, as well as other factors involved in mineral homeostasis. PTH and 1,25D mutually upregulated 36 genes and mutually downregulated 27 genes by ≥2-fold expression (P ≤ 0.05). Many identified genes were linked with the regulation of bone/tooth homeostasis, cell growth and differentiation, calcium signaling, and DMP1 transcription. Validation of RNA-seq results via PCR array confirmed a similar gene expression pattern in response to PTH and 1,25D treatment. Collectively, these results suggest that PTH and 1,25D share complementary effects in maintaining mineral homeostasis by mutual regulation of genes/proteins associated with calcium and phosphate metabolism while also exerting distinct roles on factors modulating mineral metabolism. Furthermore, PTH may modulate phosphate homeostasis by downregulating DMP1 expression via the cAMP/PKA pathway. Targeting genes/proteins mutually governed by PTH and 1,25D may be a viable approach for designing new therapies for preserving mineralized tissue health.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Wang L,Tran AB,Nociti FH Jr,Thumbigere-Math V,Foster BL,Krieger CC,Kantovitz KR,Novince CM,Koh AJ,McCauley LK,Somerman MJdoi
10.1177/0022034515599726subject
Has Abstractpub_date
2015-10-01 00:00:00pages
1408-16issue
10eissn
0022-0345issn
1544-0591pii
0022034515599726journal_volume
94pub_type
杂志文章abstract::Mandibular prognathism (MP) is a recognizable phenotype associated with dentoskeletal class III malocclusion. MP is a complex genetic trait, although familial recurrence also suggests the contribution of single inherited variations. To date, the genetic causes of MP have been investigated using linkage analysis or ass...
journal_title:Journal of dental research
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abstract::The main purpose of this paper is to review the various methods used for evaluation of fluoride retention in saliva, plaque, and enamel following application of topical anti-caries treatments such as F dentifrices and F mouthwashes. Such methods monitor delivery of fluoride to the site of action, the mouth, and so can...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/002203459207100S29
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abstract::Since the exposure of mutans streptococci to xylitol is known to select for xylitol-resistant (XR) natural mutants, the occurrence and long-term survival of such xylitol-resistant strains was evaluated in a cross-sectional sampling of participants of the Ylivieska xylitol study four years after the original two-year e...
journal_title:Journal of dental research
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abstract::Recent studies have shown that the extent of hydrolysis by plaque of the trypsin substrate, N-benzoyl-DL-arginine-2-naphthylamide (BANA), correlates with the numbers and proportions of spirochetes in subgingival plaque samples, and appears to be an indicator of clinical disease. In this study, BANA hydrolysis by subgi...
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abstract::The aim of this study was to investigate the diffusion dynamics of 25% hydrogen peroxide (H2O2) through enamel-dentin layers and to correlate it with dentin's structural alterations. Micro-Raman Spectroscopy (MRS) and Fourier Transform Infrared Photoacoustic Spectroscopy (FTIR-PAS) were used to measure the spectra of ...
journal_title:Journal of dental research
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abstract::The lack of a reliable bond test has hindered the elucidation of the mechanism for porcelain-metal bonding in dental systems, because a test capable of detecting differences among porcelain-metal bonds of various qualities is required before the reasons for these differences may be ascertained. A method was developed ...
journal_title:Journal of dental research
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abstract::The ability of human oral bacteria to inhibit Streptococcus mutans was studied under a variety of in vitro conditions using an agar overlay assay. Extensive inherent variability in microbial antagonism by certain organisms was demonstrated. Antagonistic activity was also significantly influenced by changes in the assa...
journal_title:Journal of dental research
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abstract::Eighteen different Streptococcus strains including S mutans, sanguis, mitis, salivarius, bovis, and 2F2, were assayed for their relative adsorption to synthetic hydroxyapatite and for quantities of lactic acid produced while in the adsorbed state. The results indicated that all S mutans and some S sanguis have an enha...
journal_title:Journal of dental research
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doi:10.1177/00220345770560101401
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abstract::Previous studies have demonstrated that antibodies against cholinoreceptors of exocrine glands correlate with dry mouth in persons with primary Sjögren syndrome (pSS). The aim of the present investigation was to establish if serum IgG antibodies (pSS IgG) were able to interact with cholinoreceptors in rat submandibula...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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doi:10.1177/00220345830620081801
更新日期:1983-08-01 00:00:00
abstract::In an attempt to determine the degree of co-activation present in selected cervical muscles during clenching, we instructed 12 male subjects to produce four brief maximum voluntary contraction (MVC) efforts (clenching) in a position of maximum intercuspation. Surface EMG activity was recorded bilaterally from the mass...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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abstract::Little is known about the oral health of the frail and home-living elderly. The effects of dentogenic infections on the general condition of the elderly are also unknown. We therefore set out to investigate 191 elderly patients referred to an acute geriatric hospital due to sudden worsening of their general health. Th...
journal_title:Journal of dental research
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更新日期:1997-06-01 00:00:00
abstract::Van der Woude syndrome (VWS) is an autosomal-dominant oral facial disorder. To find a gene mutation in a Japanese family using fingernail DNA samples, we performed this study. We hypothesized that a gene mutation in IRF6 might be involved in VWS, and that fingernail DNA samples may be valuable for detecting such mutat...
journal_title:Journal of dental research
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更新日期:2006-12-01 00:00:00
abstract::Dietary components rich in polyphenols-for example, tea and red wine-are thought to cause tooth staining. In the present study, hydroxyapatite was used as a model of enamel for study of the influence of salivary proteins on the binding of different polyphenols to hydroxyapatite in vitro. Neither salivary protein pelli...
journal_title:Journal of dental research
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更新日期:2005-01-01 00:00:00
abstract::Fibroblast growth factor receptor 2 (FGFR2) in craniofacial bones mediates osteoprogenitor proliferation, differentiation, and apoptosis. The distortion of proper craniofacial bone growth may cause class II and class III skeletal malocclusion and result in compromised function and aesthetics. Here, we investigated the...
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abstract::As the use of adhesive restorative materials has increased during the last several years, interest in adhesive materials that release fluoride has also grown. The purpose of this study was to measure fluoride release from several adhesive restorative materials and to evaluate its effect on dentin resistance to deminer...
journal_title:Journal of dental research
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更新日期:1999-10-01 00:00:00
abstract::Resin composites currently available are not suitable for use as large stress-bearing posterior restorations involving cusps due to their tendencies toward excessive fracture and wear. The glass fillers in composites provide only limited reinforcement because of the brittleness and low strength of glass. The aim of th...
journal_title:Journal of dental research
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abstract::Previous studies suggest that salivary and pulp-derived host enzymes, matrix metalloproteinases (MMPs), may be involved in dentin caries pathogenesis. To study the inhibition of acid-activated human salivary MMPs by non-antimicrobial chemically modified tetracyclines (CMTs), we used a functional activity assay with 12...
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abstract::Seven oral doses of demethylchlortetracycline were administered to monkeys that received serial injections of lead acetate to intravitally stain calcification sites. Bone growth was greatly inhibited, whereas dentin apposition was spared from the cumulative toxicity of demethylchlortetracycline. Cessation of bone grow...
journal_title:Journal of dental research
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更新日期:1975-03-01 00:00:00
abstract::Direct FA staining for S mutans serotypes may be performed on smears made from plaque or strain isolates and on colonies attached to agar plates of black membrane filters. Staining with single conjugates directed to S mutans serotypes (a to e) as well as with polyvalent a-, b-, d-, f-fluorescein label and c-, e-rhodam...
journal_title:Journal of dental research
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更新日期:1976-01-01 00:00:00
abstract::The purpose of the study was to determine the exposure times needed to harden a light-activated composite cement through various thicknesses of porcelain. A negative exponential model was developed with which the relative hardness of a composite could be predicted from the product of the exposure time and the square r...
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abstract::The differential diagnosis of dental wear lesions affects their clinical management. We hypothesized that surface texture parameters can differentiate simulated erosion, abrasion, and erosion-abrasion lesions on human enamel and dentin. This in vitro study comprised 2 parts (both factorial 4 × 2), with 4 lesion types ...
journal_title:Journal of dental research
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abstract::The acquired enamel pellicle is an oral, fluid-derived protein layer that forms on the tooth surface. It is a biologically and clinically important integument that protects teeth against enamel demineralization, and abrasion. Tooth surfaces are exposed to different proteinaceous microenvironments depending on the enam...
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abstract::Bone resorption beneath a maxillary complete denture was simulated by the finite element method, assuming that a threshold of compressive strain exists in the alveolar bone above which the resorption occurred. The pattern of predicted resorption was observed when 100 N of force was applied to three positions occlusall...
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abstract::Dental porcelains rely on the high-thermal-expansion mineral leucite to elevate their bulk thermal expansion to levels compatible with dental PFM alloys. The microcracks that form around these leucite particles when cooled during porcelain manufacture are a potential source of change in bulk porcelain thermal expansio...
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