Construction of a new fusion anti-caries DNA vaccine.

Abstract:

:Mutans streptococci (MS) are generally considered to be the principal etiological agent of dental caries. MS have two important virulence factors: cell- surface protein PAc and glucosyltransferases (GTFs). GTFs have two functional domains: an N-terminal catalytic sucrose-binding domain (CAT) and a C-terminal glucan-binding domain (GLU). A fusion anti-caries DNA vaccine, pGJA-P/VAX, encoding two important antigenic domains, PAc and GLU, of S. mutans, was successful in reducing the levels of dental caries caused by S. mutans in gnotobiotic animals. However, its protective effect against S. sobrinus infection proved to be weak. Does the DNA vaccine need an antigen of S. sobrinus to enhance its ability to inhibit infection? To answer this question, in this study, we cloned the catalytic (cat) fragment of S. sobrinus gtf-I, which demonstrated its ability to inhibit water-insoluble glucan synthesis by S. sobrinus, into pGJA-P/VAX to produce a new anti-caries DNA vaccine.

journal_name

J Dent Res

authors

Niu Y,Sun J,Fan M,Xu QA,Guo J,Jia R,Li Y

doi

10.1177/0022034509336727

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

455-60

issue

5

eissn

0022-0345

issn

1544-0591

pii

0022034509336727

journal_volume

88

pub_type

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