Determination of reducing power and metal chelating ability of antioxidant peptides: revisited methods.

Abstract:

:The purpose of this study was to improve two common tests used for antioxidant capacity measurements, i.e. the reducing power and chelating ability measurements, for appropriate comparisons between the molecules tested and chosen references, as the usual methods are often performed in a qualitative way rather than a quantitative way. After revision, it was then possible to determine an AERC indice (Ascorbate Equivalent Reducing Capacity) and a CECC (Carnosine Equivalent Chelating Capacity) or EECC (EDTA Equivalent Chelating Capacity) indice according to the standard chosen, by analogy to the TEAC indice (Trolox Equivalent Antioxidant Capacity) already used in many reported works to determine the free radical scavenging activity. Thus, the determination of these relative indices enables the comparison of antioxidative capacities obtained in various studies. The adaptation of these two tests to micro-scales and the calculation of AERC, EECC and CECC were performed on model peptides.

journal_name

Food Chem

journal_title

Food chemistry

authors

Canabady-Rochelle LL,Harscoat-Schiavo C,Kessler V,Aymes A,Fournier F,Girardet JM

doi

10.1016/j.foodchem.2015.02.147

subject

Has Abstract

pub_date

2015-09-15 00:00:00

pages

129-35

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(15)00352-0

journal_volume

183

pub_type

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