Abstract：
BACKGROUND:The 'attached cultivation' technique for microalgae production, combining the immobilized biofilm technology with proper light dilution strategies, has shown improved biomass production and photosynthetic efficiency over conventional open-pond suspended cultures. However, how light is transferred and distributed inside the biofilm has not been clearly defined yet. RESULTS:In this research, the growth, photosynthetic oxygen evolution, and specific growth rate for microalgal cells in both open-pond and attached cultivation were studied to determine the effective light penetration at different phases of the cultivation. As a result, the light conditions inside the culture broth as well as the biofilm were revealed for the first time. Results showed that outdoor, in a conventional 20-cm deep open pond, all of the algal cells were fully illuminated in the first 3 days of cultivation. As the biomass concentration increased from day 4 to day 10, the light could only effectively penetrate 45.5% of the open-pond depth, and then effective light penetration gradually decreased to 31.1% at day 31, when the biomass density reached a maximum value of 0.45 g L(-1) or 90 g m(-2). In the attached cultivation system, under nitrogen-replete condition, almost 100% of the immobilized algal cells inside the biofilm were effectively illuminated from day 0 through day 10 when the biomass density increased from 8.8 g m(-2) to 107.6 g m(-2). CONCLUSION:Higher light penetration efficiency might be the reason why, using attached cultivation, observed values for photosynthetic efficiency were higher than those recorded in conventional open-pond suspended cultures.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Wang J,Liu J,Liu Tdoi
10.1186/s13068-015-0240-0subject
Has Abstractpub_date
2015-03-26 00:00:00pages
49issn
1754-6834pii
240journal_volume
8pub_type
杂志文章abstract：Background:Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by converting it to xylulose. For this, the type II xylose isomerase ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1643-0
更新日期：2020-01-11 00:00:00
abstract：Background:To increase the production of bioethanol, a two-step process based on hydrothermal and dilute alkaline treatment was applied to reduce the natural resistance of biomass. However, the process required a large amount of water and a long operation time due to the solid/liquid separation before the alkaline trea...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1140-x
更新日期：2018-05-12 00:00:00
abstract：BACKGROUND:Low- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental glucose fluctuation for their growth and competitiveness. In Neurospora crassa, the dual-affinity glucose transport system consists of a low-affinity glucose transporter GLT-1 and two high-affi...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-021-01877-2
更新日期：2021-01-28 00:00:00
abstract：Background:Acetylated galactoglucomannan (AcGGM) is a complex hemicellulose found in softwoods such as Norway spruce (Picea abies). AcGGM has a large potential as a biorefinery feedstock and source of oligosaccharides for high-value industrial applications. Steam explosion is an effective method for extraction of carbo...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1300-z
更新日期：2018-11-09 00:00:00
abstract：Background:Cyanobacteria can be metabolically engineered to convert CO2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules. Results:The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobac...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-1653-y
更新日期：2020-01-28 00:00:00
abstract：Background:Understanding the extracellular electron transport pathways in cyanobacteria is a major factor towards developing biophotovoltaics. Stressing cyanobacteria cells environmentally and then probing changes in physiology or metabolism following a significant change in electron transfer rates is a common approach...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01788-8
更新日期：2020-08-26 00:00:00
abstract：BACKGROUND:Thermochemical pretreatment of lignocellulose is crucial to bioconversion in the fields of biorefinery and biofuels. However, the enzyme inhibitors in pretreatment hydrolysate make solid substrate washing and hydrolysate detoxification indispensable prior to enzymatic hydrolysis. Sulfite pretreatment to over...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-156
更新日期：2013-11-05 00:00:00
abstract：Background:Cyanobacteria are potential sources for third generation biofuels. Their capacity for biofuel production has been widely improved using metabolically engineered strains. In this study, we employed metabolic engineering design with target genes involved in selected processes including the fatty acid synthesis...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1349-8
更新日期：2019-01-04 00:00:00
abstract：BACKGROUND:Solid acid catalyzed inexpensive ionic liquid (IL) pretreatment is promising because of its effectiveness at decreasing biomass recalcitrance to subsequent enzymatic hydrolysis or in situ hydrolysis of carbohydrate oligomers. However, the conventional strategy was limited by the complex non-isothermal proces...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0589-8
更新日期：2016-08-24 00:00:00
abstract：Background:As a renewable carbon source, biomass energy not only helps in resolving the management problems of lignocellulosic wastes, but also helps to alleviate the global climate change by controlling environmental pollution raised by their generation on a large scale. However, the bottleneck problem of extensive pr...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-1648-8
更新日期：2020-01-17 00:00:00
abstract：BACKGROUND:Plant hemicellulose (largely xylan) is an excellent feedstock for renewable energy production and second only to cellulose in abundance. Beyond a source of fermentable sugars, xylan constitutes a critical polymer in the plant cell wall, where its precise role in wall assembly, maturation, and deconstruction ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0669-9
更新日期：2016-11-22 00:00:00
abstract：BACKGROUND:In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofuel from biomass is the enzymatic saccharification. Many of the commercial cellulase enzyme cocktails...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0587-x
更新日期：2016-08-22 00:00:00
abstract：BACKGROUND:When producing biofuels from dedicated feedstock, agronomic factors such as harvest time and location can impact the downstream production. Thus, this paper studies the effectiveness of ammonia fibre expansion (AFEX) pretreatment on two harvest times (July and October) and ecotypes/locations (Cave-in-Rock (C...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-3-1
更新日期：2010-01-04 00:00:00
abstract：BACKGROUND:The production of biofuels and biochemicals from grass-type plant biomass requires a complete utilisation of the plant cellulose and hemicellulosic xylan via enzymatic degradation to their constituent monosaccharides. Generally, physical and/or thermochemical pretreatments are performed to enable access for ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0519-9
更新日期：2016-05-18 00:00:00
abstract：BACKGROUND:Recently developed iron cocatalyst enhancement of dilute acid pretreatment of biomass is a promising approach for enhancing sugar release from recalcitrant lignocellulosic biomass. However, very little is known about the underlying mechanisms of this enhancement. In the current study, our aim was to identify...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-4-48
更新日期：2011-11-10 00:00:00
abstract：Background:Sustainable biofuels, which are widely considered as an attractive alternative to fossil fuels, can be generated by utilizing various biomass from the environment. Marine biomass, such as red algal biomass, is regarded as one potential renewable substrate source for biofuels conversion due to its abundance o...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1044-9
更新日期：2018-02-15 00:00:00
abstract：Background:Saccharification is the most crucial and cost-intensive process in second generation biofuel production. The deficiency of β-glucosidase in commercial enzyme leads to incomplete biomass hydrolysis. The decomposition of biomass at high temperature environments leads us to isolate thermotolerant microbes with ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0932-8
更新日期：2017-10-30 00:00:00
abstract：BACKGROUND:Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic biomass. The commercial enzyme mixtures produced at industrial scales are not well characterized, and ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-1-18
更新日期：2008-12-23 00:00:00
abstract：BACKGROUND:Brown algae are promising feedstocks for biofuel production with inherent advantages of no structural lignin, high growth rate, and no competition for land and fresh water. However, it is difficult for one microorganism to convert all components of brown algae with different oxidoreduction potentials to etha...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0494-1
更新日期：2016-04-01 00:00:00
abstract：BACKGROUND:Starch is the second most abundant plant-derived biomass and a major feedstock in non-food industrial applications and first generation biofuel production. In contrast to lignocellulose, detailed insight into fungal degradation of starch is currently lacking. This study explores the secretomes of Aspergillus...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0604-0
更新日期：2016-09-01 00:00:00
abstract：Background:A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solution of renewable routes to chemicals and fuels. Members of the genus Geobacillus are able to grow at...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1540-6
更新日期：2019-08-20 00:00:00
abstract：Background:Beer is the most popular alcoholic beverage worldwide. In the manufacture of beer, various by-products and residues are generated, and the most abundant (85% of total by-products) are spent grains. Thanks to its high (hemi)cellulose content (about 50% w/w dry weight), this secondary raw material is attractiv...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1383-1
更新日期：2019-03-05 00:00:00
abstract：Background:Black soldier fly (BSF, Hermetia illucens L.) can efficiently degrade organic wastes and transform into a high fat containing insect biomass that could be used as feedstock for biodiesel production. Meanwhile, the molecular regulatory basis of fat accumulation by BSF is still unclear; it is necessary to iden...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1531-7
更新日期：2019-08-09 00:00:00
abstract：BACKGROUND:Crude glycerol in the waste stream of the biodiesel production process is an abundant and renewable resource. However, the glycerol-based industry is usually afflicted by the cost for refinement of crude glycerol. This issue can be addressed by developing a microbial process to convert crude glycerol to valu...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0857-2
更新日期：2017-07-04 00:00:00
abstract：BACKGROUND:While microbial electrolysis cells (MECs) can simultaneously produce bioelectrochemical hydrogen and treat wastewater, they consume considerable energy to overcome the unfavorable thermodynamics, which is not sustainable and economically feasible in practical applications. This study presents a proof-of-conc...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0305-0
更新日期：2015-08-14 00:00:00
abstract：Background:Functional sugar alcohols have been widely used in the food, medicine, and pharmaceutical industries for their unique properties. Among these, erythritol is a zero calories sweetener produced by the yeast Yarrowia lipolytica. However, in wild-type strains, erythritol is produced with low productivity and yie...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-01815-8
更新日期：2020-10-20 00:00:00
abstract：BACKGROUND:Cyanobacteria are an attractive candidate for the production of biofuel because of their ability to capture carbon dioxide by photosynthesis and grow on non-arable land. However, because huge quantities of water are required for cultivation, strict water management is one of the greatest issues in algae- and...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-7-56
更新日期：2014-04-09 00:00:00
abstract：BACKGROUND:Investigations on a few eukaryotic model organisms showed that many genes are non-randomly distributed on chromosomes. In addition, chromosome ends frequently possess genes that are important for the fitness of the organisms. Trichoderma reesei is an industrial producer of enzymes for food, feed and biorefin...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0488-z
更新日期：2016-03-29 00:00:00
abstract：BACKGROUND:Solid-state anaerobic digestion (SS-AD) has been increasingly used for lignocellulosic biomass treatment. However, the separate reactor required for pretreatment prior digestion, poor treatment capacity, and process stability inhibit further development of the SS-AD. In this study, a novel method called SS-A...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0530-1
更新日期：2016-06-01 00:00:00
abstract：Background:Populus (poplar) tree species including hybrid varieties are considered as promising biomass feedstock for biofuels and biochemicals production due to their fast growing, short vegetative cycle, and widely distribution. In this work, poplar was pretreated with acetic acid (AC) to produce xylooligosaccharides...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1423-x
更新日期：2019-04-15 00:00:00
