Elevated dynorphin in the hippocampal formation of aged rats: relation to cognitive impairment on a spatial learning task.

abstract：:The effect of cytoplasm upon the expression of tumorigenicity was examined with a pair of mouse and a pair of Chinese hamster cell lines, in intraspecies cybrids formed by reciprocal fusions between either tumorigenic or nontumorigenic cells and cytoplasms derived from them. With the mouse cells, 3T3 and the simian vi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Howell AN,Sager R

更新日期：1978-05-01 00:00:00

abstract：:HeLa cells grown in the presence of [methyl-3H]thymidine contained large amounts of 5-hydroxymethyl-2'-deoxyuridine (HMdU) in their DNA. When the cells were grown in [6-3H]thymidine and their DNA was labeled to the same specific activity, no HMdU was present. When such [6-3H]thymidine-labeled cells were exposed to inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teebor GW,Frenkel K,Goldstein MS

更新日期：1984-01-01 00:00:00

abstract：:BSC-1 cells, epithelial cells of African green monkey kidney origin, show pronounced density-dependent regulation of growth in cell culture. Growth of the cells is rapid to a density of approximately 1.5 x 10(5) cells/per cm(2) in Dulbecco-modified Eagle's medium supplemented with 10% calf serum. Above this "saturatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holley RW,Armour R,Baldwin JH

更新日期：1978-01-01 00:00:00

abstract：:A system for studying Z-DNA formation in the Escherichia coli chromosome was developed. Prior investigations in recombinant plasmids showed that alternating (Pur-Pyr) sequences can adopt a left-handed Z-DNA conformation both in vitro and in vivo. We constructed mobile, transposon-based cassettes carrying cloned (Pur-P...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lukomski S,Wells RD

更新日期：1994-10-11 00:00:00

abstract：:Night-shift workers are at high risk of drowsiness-related motor vehicle crashes as a result of circadian disruption and sleep restriction. However, the impact of actual night-shift work on measures of drowsiness and driving performance while operating a real motor vehicle remains unknown. Sixteen night-shift workers ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee ML,Howard ME,Horrey WJ,Liang Y,Anderson C,Shreeve MS,O'Brien CS,Czeisler CA

更新日期：2016-01-05 00:00:00

abstract：:Enkephalins, a group of small peptides with opiate-like activity, have been defined originally as neuropeptides. Recent reports showed, using in situ hybridization, that the enkephalin-encoding gene, proenkephalin A (pEnkA), is expressed in nondifferentiated cells of diverse mesodermal lineages. The transient expressi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosen H,Polakiewicz RD,Benzakine S,Bar-Shavit Z

更新日期：1991-05-01 00:00:00

abstract：:Early acquisition of mate preferences or mate-preference learning is associated with signal diversity and speciation in a wide variety of animal species. However, the diversity of mechanisms of mate-preference learning across taxa remains poorly understood. Using the butterfly Bicyclus anynana we uncover a mechanism t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Westerman EL,Hodgins-Davis A,Dinwiddie A,Monteiro A

更新日期：2012-07-03 00:00:00

abstract：:The matrix 2 (M2) protein from influenza A virus is a proton channel that uses His37 as a selectivity filter. Here we report high-resolution (1.10 Å) cryogenic crystallographic structures of the transmembrane domain of M2 at low and high pH. These structures reveal that waters within the pore form hydrogen-bonded netw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomaston JL,Alfonso-Prieto M,Woldeyes RA,Fraser JS,Klein ML,Fiorin G,DeGrado WF

更新日期：2015-11-17 00:00:00

abstract：:The P23T mutant of human gammaD-crystallin (HGD) is associated with cataract. We have previously investigated the solution properties of this mutant, as well as those of the closely related P23V and P23S mutants, and shown that although mutations at site 23 of HGD do not produce a significant structural change in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McManus JJ,Lomakin A,Ogun O,Pande A,Basan M,Pande J,Benedek GB

更新日期：2007-10-23 00:00:00

abstract：:Lipoproteins are emulsion particles that consist of lipids and apolipoproteins. Their natural function is to transport lipids and/or cholesterol to different tissues. We have taken advantage of the hydrophobic interior of these natural emulsions to solubilize DNA. Negatively charged DNA was first complexed with cation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hara T,Tan Y,Huang L

更新日期：1997-12-23 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) represents a serious threat to the health of hospitalized patients. Attempts to reduce the spread of MRSA have largely depended on hospital hygiene and patient isolation. These measures have met with mixed success: although some countries have almost eliminated MRSA o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cooper BS,Medley GF,Stone SP,Kibbler CC,Cookson BD,Roberts JA,Duckworth G,Lai R,Ebrahim S

更新日期：2004-07-06 00:00:00

abstract：:Matching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP:NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin-Benson-Bassham (CBB) cycle. Several different mechanisms to increase ATP availabi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voon CP,Guan X,Sun Y,Sahu A,Chan MN,Gardeström P,Wagner S,Fuchs P,Nietzel T,Versaw WK,Schwarzländer M,Lim BL

更新日期：2018-11-06 00:00:00

abstract：:Hematin administered intravenously in a patient with congenital erythropoietic porphyria evidently entered erythrocyte precursors in the bone marrow, producing the well-known negative feedback repression of porphyrin biosynthesis with marked decline of porphyrin concentrations in urine, circulating plasma, and erythro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watson CJ,Bossenmaier I,Cardinal R,Petryka ZJ

更新日期：1974-02-01 00:00:00

abstract：:Restriction endonucleases such as EcoRI bind and cleave DNA with great specificity and represent a paradigm for protein-DNA interactions and molecular recognition. Using osmotic pressure to induce water release, we demonstrate the participation of bound waters in the sequence discrimination of substrate DNA by EcoRI. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson CR,Sligar SG

更新日期：1998-03-03 00:00:00

abstract：:A recombinant plasmid containing a DNA segment complementary to rat liver albumin mRNA has been constructed, cloned, and used to examine the organization of albumin gene. The 18S fraction of total liver poly(A)-containing RNA was copied into a double-stranded cDNA by avian myeloblastosis virus reverse transcriptase an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kioussis D,Hamilton R,Hanson RW,Tilghman SM,Taylor JM

更新日期：1979-09-01 00:00:00

abstract：:Hypothalamic malonyl-CoA has been shown to function in global energy homeostasis by modulating food intake and energy expenditure. Little is known, however, about the regulation of malonyl-CoA concentration in the central nervous system. To address this issue we investigated the response of putative intermediates in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolfgang MJ,Cha SH,Sidhaye A,Chohnan S,Cline G,Shulman GI,Lane MD

更新日期：2007-12-04 00:00:00

abstract：:Lipoxin B (LXB) was prepared by incubation of (15S)-15-hydroperoxy-5,8,11-cis-13-trans-icosatetraenoic acid (15-HPETE) with human leukocytes. Comparison with a number of trihydroxyicosatetraenes prepared by total synthesis showed that biologically derived LXB is (5S,14R,15S)-5,14,15-trihydroxy-6,10,12-trans-8-cis-icos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Serhan CN,Hamberg M,Samuelsson B,Morris J,Wishka DG

更新日期：1986-04-01 00:00:00

abstract：:Wildfire can influence climate directly and indirectly, but little is known about the relationships between wildfire and climate during the Quaternary, especially how wildfire patterns varied over glacial-interglacial cycles. Here, we present a high-resolution soot record from the Chinese Loess Plateau; this is a reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han Y,An Z,Marlon JR,Bradley RS,Zhan C,Arimoto R,Sun Y,Zhou W,Wu F,Wang Q,Burr GS,Cao J

更新日期：2020-03-10 00:00:00

abstract：:Productive T cell recognition of antigen-presenting cells (APCs) is normally accompanied by the formation of a cell-cell contact called the "immunological synapse." Our understanding of the steps leading up to this formation has been limited by the absence of tools for analyzing 3D surfaces and surface distributions a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moss WC,Irvine DJ,Davis MM,Krummel MF

更新日期：2002-11-12 00:00:00

abstract：:The crystal structure of the decapeptide Boc-Aib-Glu(OBzl)-Leu-Aib-Ala-Leu-Aib-Ala-Lys(Z)-Aib-OMe (where Aib is alpha-aminoisobutyryl, Boc is t-butoxycarbonyl, OBzl is benzyl ester, and Z is benzyloxycarbonyl) illustrates a parallel zipper arrangement of interacting helical peptide columns. Head-to-tail NH...OC hydrog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karle IL,Flippen-Anderson JL,Uma K,Balaram P

更新日期：1990-10-01 00:00:00

abstract：:A sensitive, labor-saving, and easily automatable nonradioactive procedure named APEX-FCS (amplified probe extension detected by fluorescence correlation spectroscopy) has been established to detect specific in vitro amplification of pathogen genomic sequences. As an example, Mycobacterium tuberculosis genomic DNA was...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walter NG,Schwille P,Eigen M

更新日期：1996-11-12 00:00:00

abstract：:(2'-5')Oligoadenylate synthetase [(2'-5')A synthetase], which synthesizes a series of oligoadenylates ppp-(A2'p)n5'A [collectively referred to as (2'-5')A], has been described previously in rat liver cells, where its concentration varied with the growth status of this organ--i.e., it decreased during the early phase o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Etienne-Smekens M,Vandenbussche P,Content J,Dumont JE

更新日期：1983-08-01 00:00:00

abstract：:The RNA-directed DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase EC 2.7.7.7) of avian oncornavirus requires a tryptophan tRNA (tRNATrp) primer molecule located close to the 5' end of the viral RNA genome for the initiation of DNA synthesis in vitro. In this communication we demonstrate that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collett MS,Faras AJ

更新日期：1976-04-01 00:00:00

abstract：:A search for potent toxins that inhibit neuronal calcium channels in Drosophila melanogaster has resulted in the identification of a presynaptic neurotoxin from the venom of the hunting spider Hololena curta. Using Drosophila neuromuscular junction as an assay, presynaptic inhibitory activity was purified using gel fi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowers CW,Phillips HS,Lee P,Jan YN,Jan LY

更新日期：1987-05-01 00:00:00

abstract：:The thymidine analog fialuridine deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil (FIAU) was toxic in trials for chronic hepatitis B infection. One mechanism postulated that defective mtDNA replication was mediated through inhibition of DNA polymerase-gamma (DNA pol-gamma), by FIAU triphosphate (FIALTP) or by trip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis W,Levine ES,Griniuviene B,Tankersley KO,Colacino JM,Sommadossi JP,Watanabe KA,Perrino FW

更新日期：1996-04-16 00:00:00

abstract：:Electron microscopy is arguably the most powerful tool for spatial imaging of structures. As such, 2D and 3D microscopies provide static structures with subnanometer and increasingly with angstrom-scale spatial resolution. Here we report the development of 4D ultrafast electron microscopy, whose capability imparts ano...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lobastov VA,Srinivasan R,Zewail AH

更新日期：2005-05-17 00:00:00

abstract：:Novel organismal structures in metazoans are often undergirded by complex gene regulatory networks; as such, understanding the emergence of new structures through evolution requires reconstructing the series of evolutionary steps leading to these underlying networks. Here, we reconstruct the step-by-step assembly of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irimia M,Denuc A,Burguera D,Somorjai I,Martín-Durán JM,Genikhovich G,Jimenez-Delgado S,Technau U,Roy SW,Marfany G,Garcia-Fernàndez J

更新日期：2011-03-29 00:00:00

abstract：:Mosquito repellency data on acylpiperidines derived from the U.S. Department of Agriculture archives were modeled by using molecular descriptors calculated by CODESSA PRO software. An artificial neural network model was developed for the correlation of these archival results and used to predict the repellent activity ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katritzky AR,Wang Z,Slavov S,Tsikolia M,Dobchev D,Akhmedov NG,Hall CD,Bernier UR,Clark GG,Linthicum KJ

更新日期：2008-05-27 00:00:00

abstract：:Myosin-binding protein C (MyBP-C) is an accessory protein of striated muscle thick filaments and a modulator of cardiac muscle contraction. Defects in the cardiac isoform, cMyBP-C, cause heart disease. cMyBP-C includes 11 Ig- and fibronectin-like domains and a cMyBP-C-specific motif. In vitro studies show that in addi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mun JY,Previs MJ,Yu HY,Gulick J,Tobacman LS,Beck Previs S,Robbins J,Warshaw DM,Craig R

更新日期：2014-02-11 00:00:00

abstract：:Guanosine tetraphosphate, recently discovered to mediate the regulatory relationship between protein synthesis and RNA accumulation in various bacteria, has been synthesized in vitro in large quantities and analyzed by natural-abundance (13)C nuclear magnetic resonance spectroscopy in order to confirm its structure an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Que L Jr,Willie GR,Cashel M,Bodley JW,Gray GR

更新日期：1973-09-01 00:00:00