Abstract：
:Brain networks contain a large diversity of functionally distinct neuronal elements, each with unique properties, enabling computational capacities and supporting brain functions. Understanding their functional implications for behavior requires the precise identification of the cell types of a network and in vivo monitoring of their activity profiles. Here, we developed a channelrhodopsin-assisted patching method allowing the efficient in vivo targeted recording of neurons identified by their molecular, electrophysiological, and morphological features. The method has a high yield, does not require visual guidance, and thus can be applied at any depth in the brain. This approach overcomes limitations of present technologies. We validate this strategy with in vivo recordings of identified subtypes of GABAergic and glutamatergic neurons in deep cortical layers, subcortical cholinergic neurons, and neurons in the thalamic reticular nucleus in anesthetized and awake mice. We propose this method as an important complement to existing technologies to relate specific cell-type activity to brain circuitry, function, and behavior.
journal_name
Cell Repjournal_title
Cell reportsauthors
Muñoz W,Tremblay R,Rudy Bdoi
10.1016/j.celrep.2014.11.042subject
Has Abstractpub_date
2014-12-24 00:00:00pages
2304-16issue
6issn
2211-1247pii
S2211-1247(14)01008-0journal_volume
9pub_type
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