当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > A G-quadruplex DNA-based, label-free and ultrasensitive strategy for microRNA detection.

A G-quadruplex DNA-based, label-free and ultrasensitive strategy for microRNA detection.

Abstract：

:MicroRNAs (miRNAs) have been considered to be potent biomarkers for early disease diagnosis and for cancer therapy. The rapid and selective detection of miRNAs without reverse transcription and labelling is highly desired. Herein, we report a simple and label-free miRNA detection method that is based on the Duplex-Specific Nuclease (DSN)-Assisted simple target miRNA recycling procedure. The interaction of the G-quadruplex DNA structure with N-methyl mesoporphyrin IX (NMM) led to a label-free signal output. Under the optimised conditions, this method allowed for simple, rapid, and sequence-specific detection of miR-141 over a dynamic range from 1 fM to 100 nM with a linear range from 1 pM to 100 nM. Moreover, our method offered an excellent capacity to discriminate between miRNA family members with just one mismatched nucleotide. This simple and label-free strategy holds great potential in applications in biomedical research and in early clinical diagnostics.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Yan L,Yan Y,Pei L,Wei W,Zhao J

doi

10.1038/srep07400

subject

Has Abstract

pub_date

2014-12-10 00:00:00

pages

7400

issn

2045-2322

pii

srep07400

journal_volume

4

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • HARDI-ZOOMit protocol improves specificity to microstructural changes in presymptomatic myelopathy.

    abstract：:Diffusion magnetic resonance imaging (dMRI) proved promising in patients with non-myelopathic degenerative cervical cord compression (NMDCCC), i.e., without clinically manifested myelopathy. Aim of the study is to present a fast multi-shell HARDI-ZOOMit dMRI protocol and validate its usability to detect microstructura...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-70297-3

    authors： Labounek R,Valošek J,Horák T,Svátková A,Bednařík P,Vojtíšek L,Horáková M,Nestrašil I,Lenglet C,Cohen-Adad J,Bednařík J,Hluštík P

    更新日期：2020-10-16 00:00:00

  • Interconnecting Carbon Fibers with the In-situ Electrochemically Exfoliated Graphene as Advanced Binder-free Electrode Materials for Flexible Supercapacitor.

    abstract：:Flexible energy storage devices are highly demanded for various applications. Carbon cloth (CC) woven by carbon fibers (CFs) is typically used as electrode or current collector for flexible devices. The low surface area of CC and the presence of big gaps (ca. micro-size) between individual CFs lead to poor performance...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep11792

    authors： Zou Y,Wang S

    更新日期：2015-07-07 00:00:00

  • A new hand-held microfluidic cytometer for evaluating irradiation damage by analysis of the damaged cells distribution.

    abstract：:Space radiation brings uneven damages to cells. The detection of the distribution of cell damage plays a very important role in radiation medicine and the related research. In this paper, a new hand-held microfluidic flow cytometer was developed to evaluate the degree of radiation damage of cells. The device we propos...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep23165

    authors： Wang J,Fan Z,Zhao Y,Song Y,Chu H,Song W,Song Y,Pan X,Sun Y,Li D

    更新日期：2016-03-17 00:00:00

  • First detection of white spot syndrome virus (WSSV) in the mud shrimp Austinogebia edulis in Taiwan.

    abstract：:The white spot syndrome virus (WSSV) causes mass mortalities in the aquaculture of shrimps worldwide. The mud shrimp Austinogebia edulis (Ngoc-Ho & Chan, 1992) is an economically important sea food item occurring along the west coast of Taiwan. While the population of A. edulis began to decrease with some fluctuations...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-54837-0

    authors： Zhu F,Twan WH,Tseng LC,Peng SH,Hwang JS

    更新日期：2019-12-09 00:00:00

  • Previous flight facilitates partner finding in female crickets.

    abstract：:In the cricket Gryllus bimaculatus, flying occurs soon after the last imaginal molt and precedes the mating behavior in natural conditions. Here, we tested the hypothesis that flying may improve subsequent behavioral performance in a novel environment in female crickets. We developed a behavioral set-up to test female...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-78969-w

    authors： Mezheritskiy M,Vorontsov D,Lapshin D,Dyakonova V

    更新日期：2020-12-18 00:00:00

  • Transcriptome profiling of spike provides expression features of genes related to terpene biosynthesis in lavender.

    abstract：:Lavender (Lavandula angustifolia) is an important economic plant because of the value of its essential oil (EO). The Yili Valley in Xinjiang has become the largest lavender planting base in China. However, there is a lack of research on the gene expression regulation of EO biosynthesis and metabolism in local varietie...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-63950-4

    authors： Guo D,Kang K,Wang P,Li M,Huang X

    更新日期：2020-04-24 00:00:00

  • Alleviation of depression-like behavior in a cystic fibrosis mouse model by Hdac6 depletion.

    abstract：:Cystic fibrosis (CF) patients experience heightened levels of anxiety and depression. Stress from dealing with chronic disease and rigorous treatment regimens certainly are primary contributors to these outcomes. We previously have demonstrated that microtubule alterations in CF are linked to a number of CF phenotypes...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-73298-4

    authors： Corey DA,Rymut SM,Kelley TJ

    更新日期：2020-10-01 00:00:00

  • Differential levels of Neurofilament Light protein in cerebrospinal fluid in patients with a wide range of neurodegenerative disorders.

    abstract：:Cerebrospinal fluid (CSF) biomarkers are useful in the diagnosis and the prediction of progression of several neurodegenerative diseases. Among them, CSF neurofilament light (NfL) protein has particular interest, as its levels reflect neuroaxonal degeneration, a common feature in various neurodegenerative diseases. In...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66090-x

    authors： Delaby C,Alcolea D,Carmona-Iragui M,Illán-Gala I,Morenas-Rodríguez E,Barroeta I,Altuna M,Estellés T,Santos-Santos M,Turon-Sans J,Muñoz L,Ribosa-Nogué R,Sala-Matavera I,Sánchez-Saudinos B,Subirana A,Videla L,Benejam B,Si

    更新日期：2020-06-08 00:00:00

  • Optimal SSSC-based power damping inter-area oscillations using firefly and harmony search algorithms.

    abstract：:The static synchronous series compensator (SSSC) can add a series reactance to the transmission line, and when it is fed using auxiliary signals, it can participate in damping inter-area oscillations by changing the series reactance. In this paper, the effect of the SSSC on small-signal stability is investigated. The ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-69123-7

    authors： Naderipour A,Abdul-Malek Z,Ramachandaramurthy VK,Miveh MR,Moghaddam MJH,Guerrero JM

    更新日期：2020-07-22 00:00:00

  • Field cress genome mapping: Integrating linkage and comparative maps with cytogenetic analysis for rDNA carrying chromosomes.

    abstract：:Field cress (Lepidium campestre L.), despite its potential as a sustainable alternative oilseed plant, has been underutilized, and no prior attempts to characterize the genome at the genetic or molecular cytogenetic level have been conducted. Genetic maps are the foundation for anchoring and orienting annotated genome...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-53320-0

    authors： Desta ZA,Kolano B,Shamim Z,Armstrong SJ,Rewers M,Sliwinska E,Kushwaha SK,Parkin IAP,Ortiz R,de Koning DJ

    更新日期：2019-11-19 00:00:00

  • Waterborne aripiprazole blunts the stress response in zebrafish.

    abstract：:Here we provide, at least to our knowledge, the first evidence that aripiprazole (APPZ) in the water blunts the stress response of exposed fish in a concentration ten times lower than the concentration detected in the environment. Although the mechanism of APPZ in the neuroendocrine axis is not yet determined, our res...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep37612

    authors： Barcellos HH,Kalichak F,da Rosa JG,Oliveira TA,Koakoski G,Idalencio R,de Abreu MS,Giacomini AC,Fagundes M,Variani C,Rossini M,Piato AL,Barcellos LJ

    更新日期：2016-11-22 00:00:00

  • Modified recombinant human erythropoietin with potentially reduced immunogenicity.

    abstract：:Recombinant human erythropoietin (rHuEPO) is a biopharmaceutical drug given to patients who have a low hemoglobin related to chronic kidney disease, cancer or anemia. However, some patients repeatedly receiving rHuEPO develop anti-rHuEPO neutralizing antibodies leading to the development of pure red cell aplasia (PRCA...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-80402-1

    authors： Susantad T,Fuangthong M,Tharakaraman K,Tit-Oon P,Ruchirawat M,Sasisekharan R

    更新日期：2021-01-15 00:00:00

  • Clostridium difficile: New Insights into the Evolution of the Pathogenicity Locus.

    abstract：:The major virulence factors of Clostridium difficile are toxins A and B. These toxins are encoded by tcdA and tcdB genes, which form a pathogenicity locus (PaLoc) together with three additional genes that have been implicated in regulation (tcdR and tcdC) and secretion (tcdE). To date, the PaLoc has always been found ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep15023

    authors： Monot M,Eckert C,Lemire A,Hamiot A,Dubois T,Tessier C,Dumoulard B,Hamel B,Petit A,Lalande V,Ma L,Bouchier C,Barbut F,Dupuy B

    更新日期：2015-10-08 00:00:00

  • Sarcodia suieae acetyl-xylogalactan regulate RAW 264.7 macrophage NF-kappa B activation and IL-1 beta cytokine production in macrophage polarization.

    abstract：:In this study, the effects of acetyl-xylogalactan extracted from Sarcodia suieae on RAW 264.7 macrophage polarisation were evaluated. This extracted acetyl-xylogalactan had a monosaccharide composition of 91% galactose and 9% xylose, with polysaccharide and acetyl contents of 80.6% and 19.3%, respectively. MALDI-TOF m...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56246-9

    authors： Wu TM,Nan FH,Chen KC,Wu YS

    更新日期：2019-12-23 00:00:00

  • Expression of NOTCH3 exon 16 differentiates Diffuse Large B-cell Lymphoma into molecular subtypes and is associated with prognosis.

    abstract：:Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease with diverse clinical presentation and outcome. Bio-clinical prognostic models including oncogene expression and cell-of-origin phenotyping has been developed, however, approximately 30% of all patients still die from their disease, illustrating the need...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36680-x

    authors： Jespersen DS,Schönherz AA,Due H,Bøgsted M,Sondergaard TE,Dybkær K

    更新日期：2019-01-23 00:00:00

  • Nanocomposite Metamaterials Based on Self-assembled Titanium Dioxide Rolls with Embedded Gold Nanoparticles.

    abstract：:An experimental method for fabrication of a nanocomposite metamaterial based on a self-assembly of titanium dioxide microtubes with encapsulated gold nanoparticles (NPs) is proposed. The formation of microtubes is induced by laser irradiation in the presence of an external magnetic field. It is shown that the variatio...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43588-7

    authors： Kutrovskaya S,Kucherik A,Osipov A,Samyshkin V,Istratov A,Kavokin AV

    更新日期：2019-05-07 00:00:00

  • Vitamin D deficiency serves as a precursor to stunted growth and central adiposity in zebrafish.

    abstract：:Emerging evidence demonstrates the importance of sufficient vitamin D (1α, 25-dihydroxyvitamin D3) levels during early life stage development with deficiencies associated with long-term effects into adulthood. While vitamin D has traditionally been associated with mineral ion homeostasis, accumulating evidence suggest...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-72622-2

    authors： Knuth MM,Mahapatra D,Jima D,Wan D,Hammock BD,Law M,Kullman SW

    更新日期：2020-09-29 00:00:00

  • A prospective, randomised study of the effect of fixation sutures during phacotrabeculectomy on intraocular pressure and incidence of ptosis.

    abstract：:We investigated the effects of different intraoperative eyeball fixation techniques (superior rectus muscle suture [MS] and traction suture at the corneal limbus [CS]), on intraocular pressure (IOP) and the incidence of ptosis after phacotrabeculectomy. Forty-one eyes with different glaucoma types which qualified for ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-79635-x

    authors： Konopińska J,Lisowski Ł,Mariak Z,Wojnar M,Obuchowska I,Rękas M

    更新日期：2021-01-12 00:00:00

  • Ecosystem responses to warming and watering in typical and desert steppes.

    abstract：:Global warming is projected to continue, leading to intense fluctuations in precipitation and heat waves and thereby affecting the productivity and the relevant biological processes of grassland ecosystems. Here, we determined the functional responses to warming and altered precipitation in both typical and desert ste...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep34801

    authors： Xu Z,Hou Y,Zhang L,Liu T,Zhou G

    更新日期：2016-10-10 00:00:00

  • Increased systolic blood pressure reactivity to acute stress is related with better self-reported health.

    abstract：:The stress reactivity hypothesis posits that the magnitude of cardiovascular reactions to acute stress tasks is related with future blood pressure status, heart hypertrophy, and atherosclerosis. We assessed the stress reactivity hypothesis and aimed to identify which physiological indices (blood pressure, heart-rate, ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep06882

    authors： Wright BJ,O'Brien S,Hazi A,Kent S

    更新日期：2014-11-13 00:00:00

  • Maternal lipid levels across pregnancy impact the umbilical cord blood lipidome and infant birth weight.

    abstract：:Major alterations in metabolism occur during pregnancy enabling the mother to provide adequate nutrients to support infant development, affecting birth weight (BW) and potentially long-term risk of obesity and cardiometabolic disease. We classified dynamic changes in the maternal lipidome during pregnancy and identifi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71081-z

    authors： LaBarre JL,Puttabyatappa M,Song PXK,Goodrich JM,Zhou L,Rajendiran TM,Soni T,Domino SE,Treadwell MC,Dolinoy DC,Padmanabhan V,Burant CF

    更新日期：2020-08-26 00:00:00

  • Complementary Role of Fibroblast Growth Factor 21 and Cytokeratin 18 in Monitoring the Different Stages of Nonalcoholic Fatty Liver Disease.

    abstract：:Fibroblast growth factor 21 (FGF21) and cytokeratin 18 (CK18) were previously reported to be elevated in nonalcoholic fatty liver disease (NAFLD). We aim to analyze the differential roles of FGF21, cell apoptosis marker CK18 fragment M30 and total cell death marker CK18 M65ED in monitoring the different stages of NAFL...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-05257-5

    authors： Wu G,Li H,Fang Q,Zhang J,Zhang M,Zhang L,Wu L,Hou X,Lu J,Bao Y,Jia W

    更新日期：2017-07-11 00:00:00

  • Carbon: nitrogen stoichiometry following afforestation: a global synthesis.

    abstract：:Though carbon (C): nitrogen (N) stoichiometry has been widely studied in terrestrial ecosystems, little is known about its variation following afforestation. By synthesizing the results of 53 studies, we examined temporal and spatial variation in C: N ratios and in N-C scaling relationships of both the organic and the...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep19117

    authors： Xu X,Li D,Cheng X,Ruan H,Luo Y

    更新日期：2016-01-08 00:00:00

  • Human oral isolate Lactobacillus fermentum AGR1487 induces a pro-inflammatory response in germ-free rat colons.

    abstract：:Lactobacilli are thought to be beneficial for human health, with lactobacilli-associated infections being confined to immune-compromised individuals. However, Lactobacillus fermentum AGR1487 negatively affects barrier integrity in vitro so we hypothesized that it caused a pro-inflammatory response in the host. We comp...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20318

    authors： Anderson RC,Ulluwishewa D,Young W,Ryan LJ,Henderson G,Meijerink M,Maier E,Wells JM,Roy NC

    更新日期：2016-02-04 00:00:00

  • Experimental study on the location of an acoustic emission source considering refraction in different media.

    abstract：:The existing acoustic emission (AE) source location methods assume that acoustic waves propagate along straight lines, and the source location is determined by average wave velocity. Because of the heterogeneity of materials, location results often fail to meet the accuracy requirement. For this reason, an AE source l...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07371-w

    authors： Zhou ZL,Zhou J,Dong LJ,Cai X,Rui YC,Ke CT

    更新日期：2017-08-07 00:00:00

  • A 64-pin Nanowire Surface Fastener Like a Ball Grid Array Applied for Room-temperature Electrical Bonding.

    abstract：:Surface-mount techniques primarily depend on soldering. However, soldering techniques have encountered some challenges in recent years. These challenges include rare metal recycling, thermal problems, and Pb toxicity. We recently developed a metallic nanowire surface fastener (NSF) to resolve the abovementioned proble...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37693-2

    authors： Toku Y,Ichioka K,Morita Y,Ju Y

    更新日期：2019-01-31 00:00:00

  • A multi-scale model for determining the effects of pathophysiology and metabolic disorders on tumor growth.

    abstract：:The search for efficient chemotherapy drugs and other anti-cancer treatments would benefit from a deeper understanding of the tumor microenvironment (TME) and its role in tumor progression. Because in vivo experimental methods are unable to isolate or control individual factors of the TME and in vitro models often do ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-59658-0

    authors： Nikmaneshi MR,Firoozabadi B,Mozafari A,Munn LL

    更新日期：2020-02-20 00:00:00

  • Differential evolution algorithm for performance optimization of the micro plasma actuator as a microelectromechanical system.

    abstract：:Dielectric Discharge Barrier (DBD) plasma actuators are considered as one of the best active electro-hydrodynamic control devices, and are considered by many contemporary researchers. Here a simple electrostatic model, which is improved by authors, and uses the Maxwell's and the Navier-Stokes equations, is proposed fo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-75419-5

    authors： Omidi J,Mazaheri K

    更新日期：2020-11-02 00:00:00

  • Low-Dose Tacrolimus Prevents Dysregulated Peri-Conceptional Ovarian and Systemic Immune Cellular Homeostasis in Subjects with PCOS.

    abstract：:Polycystic ovary syndrome (PCOS) is characterized by failure of ovulation and is associated with obesity and chronic inflammation. Recent evidence suggests that anomalous activation of ovarian macrophages and numerical and functional deficits in the Th17 (CD4+IL17A+) and the CD4+CD25+CD127low Tregs plays crucial role ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-42960-x

    authors： Albaghdadi AJH,Feeley CA,Kan FWK

    更新日期：2019-04-25 00:00:00

  • Visualization of synaptic domains in the Drosophila brain by magnetic resonance microscopy at 10 micron isotropic resolution.

    abstract：:Understanding the complex architecture, connectivity, and pathology of the human brain is a major application of magnetic resonance imaging (MRI). However, the cellular basis of MR signal is still poorly understood. The advent of MR microscopy (MRM) enables imaging biological samples at cellular resolution, helping to...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep08920

    authors： Lee CH,Blackband SJ,Fernandez-Funez P

    更新日期：2015-03-10 00:00:00